• Applied Biological Chemistry
• /
• v.50 no.4
• /
• pp.316-320
• /
• 2007

Three phytotoxic compounds were isolated from the culture broth of Chaetomium sp. through silica gel column chromatography and HPLC (RP-18). Their chemical structures were elucidated as chaetoglobosin F, chaetoglobosin C and chaetoglobosin E on the basis of instrumental analyses such as $^1H-NMR,\;^{13}C-NMR$, and HMQC. They inhibited the root growth of barnyard grass with the $IC_{50}$ values of 66, 65, and $67{\mu}g/ml$, respectively.

• Korean Institute of Interior Design Journal
• /
• no.7
• /
• pp.31-37
• /
• 1996

The purpose of this study is to grasp the characteristics by image type in interior color. This experiment is carried out by the evaluation method of color simulation with the color image processor. And the result of this experimental evaluation is analyzed quantitatively by HAYASI 1 Program. The results of this analysis are as follows : 1) In casual and clear images, the most main colors are GY, PB, Y , and N, Casual image has high chroma and the most arrangement of colors is hue-contrast or contrast -harmony with white. Also the main colors of clear image are in identical or similar harmony with the hues of floor. 2) In romantic, elegant, pretty, and gorgeous images, the most main colors are GY, RP, R , YR and Y and the most arrangement of colors is identical or similar harmony. The romantic image of pastel tone is wholly lighter than the pretty image of bright tone. And elegant image is lower in chroma than romantic images, so generally dark. Also gorgeous image is the vivid tone with high chroma. 3) In chic and modern images, the main colors are the hues of B, PB, high value and low chroma with bright tone. Also, the main colors are in identical or similar harmony with the hues of floor : BG , B, PB and P. 4) In natural and semiclassic images, the main color is the warm color of Y, YR and the most arrangement of colors is identical or similar harmony. Also Semiclassic images is the dull tone with middle value and middle chroma and darker tone than natural image. 5) In dynamic image, the main color is the hue of N, Y, PB and GY and most of color is high chroma. And the most arrangement of colors is value-contrast.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.37 no.7
• /
• pp.984-999
• /
• 2013

This research examines the interrelation of clothes, colors and sentiments based on clothes and colors that stimulate sentiment. This study provides data that is useful to color therapy by means of clothes as medium. The survey for this study targeted 200 Pusan National University students who analyzed the colors of association and clothes colors for nine positive vocabularies (passion, love, warmth, happiness, interest, softness, comfortable, freshness, and coolness) and six negative vocabularies (anger, fear, despair, nervous, gloomy, and loneliness). The data collection process used 120 standard colors as represented by Munsell's basic 10 colors (R, YR, Y, GY, G, BG, B, PB, P, RP) as chromatic colors classified into eleven tones of colors (V, S, B, P, VP, LGR, GR, L, DL, DP, DK) and achromatic colors divided into ten steps of brightness N1-N10. The results of the research are as follows. First, the warm class of colors were significant in the colors of association with positive sentiment and the cold class of colors were significant in the sentiment of refreshment and coolness. In addition, bright and clear colors (like V, S, VP, P) were associated with color tones. Second, the low bright achromatic colors were generally high for the colors of association with negative sentiment; in addition, the color of R, PB, P (as achromatic colors) were also significant. In addition, sober and dark tones (like GR, DL, DK, DP) were significant in color tones. Third, the interrelation between positive sentiment and clothes colors shows that colors of association were mainly used for upper garment colors. Similar colors against upper garments were used together for bottom garment achromatic colors and complementary colors; therefore, bottom garments play a subsidiary role in the concept of coordination with upper garments.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
• /
• v.23 no.1
• /
• pp.103-114
• /
• 1993

The purpose of this study was to determine whether any difference existed in craniofacial morphology between parents of children with cleft lip and/or palate and parents of children without cleft lip and/or palate as well as the characteristics of craniofacial morphology in parents of children with cleft lip and/or palate. Thirty three measurements of the various regions of cranium and face were obtained from lateral cephalometric radiograms in parents of 28 children with cleft lip and palate, 18 children with cleft lip, and 22 children with cleft palate. There were 28 couples and 40 single parents in this sample. There were 92 individuals including 41 males and 51 females. The measurements were compared with those in control subjects, including 40 adult males and 40 adult females, who had no history of craniofacial abnormalities. The total sample was compared for the sex independently. The obtained results were as follows. 1. In the cranium, both parents of cleft children had significantly shorter posterior cranial base length(S-Ba). 2. In the upper face, a significantly shorter anteroposterior length of maxilla(A'-Ptm'), particularly in the anterior region (A'-K), anterior facial depth(A-SBaL), posterior facia! height(Ptm'-SNL) and relation of subnasale to the cranial base (∠BaN'Sn) were noted in fathers of cleft children. But, all measurements were not found to be significantly different between experimental group and control group in all mothers. 3. In the lower face, both parents of cleft children showed a significantly greater Y axis angle(∠NSGn) and ramal plane angle(∠SNL-RP) in fathers of cleft children. Thus both patents showed a posteriorly rotation of mandible. The thickness of the lower lip(B-B') was significantly thicker in fathers of cleft children. 4. In the facial profile, a significantly shorter posterior facial height(S-Go) and greater angle of soft tissue facial convexity (∠BaN'Pog') were noted in the fathers of cleft children. But, all measurements were not found to be significantly different between experimental group and control group in all mothers.

• Korean Journal of Veterinary Service
• /
• v.44 no.3
• /
• pp.163-168
• /
• 2021

The rapid and reliable detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a key role in isolating infected patients and preventing further viral transmission. In this study, we evaluated the clinical diagnostic performances of a commercial real-time reverse transcription loop-mediated isothermal amplification (RRT-LAMP) assay (Isopollo^® COVID-2 assay, M-monitor, Daegu, Korea) using eighty COVID-19 suspected clinical samples and compared these with the results of a commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) assay (Allplex^TM 2019-nCoV rRT-QPCR Assay, SeeGene, Seoul, Korea). The results of the RRT-LAMP assay targeting the N or RdRp gene of SARS-CoV-2 showed perfect agreement with the RT-qPCR assay results in terms of detection. Furthermore, the RRT-LAMP assay was completed in just within a 20-min reaction time, which is significantly faster than about the 2 h currently required for the RT-qPCR assay, thus enabling prompt decision making regarding the isolation of infected patients. The RRT-LAMP assay will be a valuable tool for rapid, sensitive, and specific detection of SARS-CoV-2 in human or unexpected animal clinical cases.

• Analytical Science and Technology
• /
• v.36 no.1
• /
• pp.32-43
• /
• 2023

This study reports for the first time about a stability indicating RP-HPLC method for qualitative and quantitative determination of acalabrutinib in bulk and dosage form and in presence its impurities 1, 2 and 3. The chromatographic separation was carried on Zorbax XDB-C18 (250×4.6 mm; 5 µ id) as stationary phase, Phosphate buffer pH 6.4 and methanol 80:20 (v/v) as mobile phase at a flow rate of 1.0 mL/min, UV detection was carried at wavelength of 238 nm and the analysis was completed with a run time of 15 min. In these conditions the retention time of acalabrutinib and its impurities 1, 2 and 3 was observed to be 3.50, 4.83, 8.40 and 9.93 min respectively. The method was validated for system suitability, range of analysis, precision, specificity, stability and robustness. Spiked recovery at 50 %, 100 % and 150 % was carried for both standard and impurities and the acceptable % recovery of 98-102 was observed for acalabrutinib and both impurities studied and the % RSD in each spiked level was found to be less than 2. Stability tests were done through exposure of the analyte solution to five different stress conditions i.e expose to 1N hydrochloric acid, 1 N sodium hydroxide, 3 % peroxide, 80 ℃ temperature and UV radiation at 254 nm. In all the degradation condition, standard drug acalabrutinib was detected along with both the impurities studied and the degradation products were successfully separated. In the formulation analysis there is no other chromatographic detection of other impurities and formulation excipients. Hence the developed method was found to be suitable for the quantification of acalabrutinib and can separate and analyse impurities 1 and 2.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.33 no.2
• /
• pp.153-157
• /
• 2000

Hydrolysate which inhibit the Angiotensin I-converting enzyme (ACE) was prepared from mackerel muscle by pretense. The ACE inhibitory activity of mackerel muscle hvdrolysate (MMH) was $967 {\mu}g of IC_(50)$. ACE inhibitory peptides were isolated by ultrafiltration, gel permeation column chromatography (GPC), reversed phase column chromatography(RPC), reversed phasehigh performance liquid chromatography (RP-HPLC), and gel permeation high performance liquid chromatography (GP-HPLC) from the MMH. The amino acid sequence of the ACE inhibitory peptides was Tyr-Val-Ala. The $IC_(50)$ of this peptide for ACE from rabbit lung was $1.4 {\mu}M$.

• Korean Journal of Soil Science and Fertilizer
• /
• v.31 no.2
• /
• pp.128-136
• /
• 1998

Most of total nitrogen in the surface soil exists in organic forms, of which amino acid-N is the major fraction. By-product fertilizers provide soil with humic substances, and humic acid is an essential component of humus. Amino acids(AAs) are easily converted to inorganic-N forms and thus play an important role in N fertility. This experiment was conducted to investigate the contents and distributions of AAs in humic acids which were extracted from the commercial by-product fertilizers of different composting materials. Total contents of AAs in humic acids ranged from 1.2 to 5.6%, of which neutral AAs were the highest with ranges of 0.8~4.5%. AAs contents in fertilizers composted from the plant residues such as leaf litter, sawdust and bark were in an order of neutral>acidic>basic AAs. In contrast, those from animal wastes, such as poultry and pig manures, were in an order of neutral>basic>acidic AAs. Distributions of total, acidic and neutral AAs were in the respective order of leaf litter>sawdust>pig manure>poultry manure>peat, bark>sawdust>leaf litter>peat and leaf litter>sawdust>bark>peat. Distributions of the basic AAs were in the reversed order of the acidic AAs. In bark fertilizer with increasing compost maturity, contents of the acidic AAs were increased in compensation for the decreases in those of neutral and basic AAs. Results demonstrated that distributions of amino acids in humic acid of by-product fertilizers were different from composting raw materials and degrees of humification.

• Journal of Food Hygiene and Safety
• /
• v.20 no.4
• /
• pp.244-252
• /
• 2005

This study was performed far development of new analytical method of beet red in foods. In this study, analysis of beet red in foods has been carried out by detection of betanine and isobetanine, the main color component of beet red as indicator compounds. The qualitative analysis technique consisted of clean-up of the colors with a $C_{18}$ cartridge, separation of the colors by cellulose TLC plate using acetone:3-methyl-1-butanol:distilled water (7:7:6) as a solvent system. Also, the quantitative analysis was performed using X-terra RP at wavelength 538 nm and $0.1\%$ phosphoric acid : methanol (90:10) as a solvent. The quantitative results of beet .ed were as follows:900.22$\∼$27701.60 $\mu$g/g for 60 item in nutrient supplement food, $21.95\∼713.40{\mu}g/g$ for 30 items and N.D. for 18 items in cindy, and $155.85{\∼}505.37{\mu}g/g$ for 12 items in ice creams, $43.52\∼64.75{\mu}g/g$ for 18 items and N.D. for 54 item in sauce, N.D. for 12 items in retort food.

• Journal of Life Science
• /
• v.28 no.11
• /
• pp.1301-1315
• /
• 2018

We purified an antimicrobial peptide from the acidified hemocyte extract of Mytilus coruscus by $C_{18}$ reversed-phase high-performance liquid chromatography (RP-HPLC). The peptide was 4041.866 Da based on matrix-assisted laser desorption ionization time-of-flight mass spectrophotometer (MALDI-TOF/MS) and the 25 amino acids of the N-terminus sequence were identified. Comparison of this sequence of the purified peptide with the N-terminus sequences of other antimicrobial peptides revealed 100% identity with the mytilin B precursor of Mytilus coruscus. We also identified a 312 bp open-reading frame (ORF) encoding 103 amino acids based on the obtained amino acid residues. The nucleotide sequence of this ORF and the amino acid sequence also revealed 100% identity with the mytilin B precursor of Mytilus coruscus. We synthesized two antimicrobial peptides with an alanine residue in the C-terminus, and designated them mytilin B1 and B2. These two antimicrobial peptides showed antimicrobial activity against gram-positive bacteria, including Bacillus cereus and Streptococcus parauberis (minimal effective concentration, MECs $41.6-89.7{\mu}g/ml$), gram-negative bacteria, including Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Providencia stuartii, Pseudomonas aeruginosa, and Vibrio ichthyoenteri (MECs $7.4-39.5{\mu}g/ml$), and the fungus Candida albicans (MECs $26.0-31.8{\mu}g/ml$). This antimicrobial activity was stable under heat and salt conditions. Furthermore, the peptides did not exhibit significant hemolytic activity or cytotoxic effects. These results suggest that mytilin B could be applied as alternative antibiotic agent, and they add to the understanding of the innate immunity of hard-shelled mussels.