Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Phytotoxin Isolated from the Culture Broth of Chaetomium sp.

Chaetomium sp. 배양액에서 분리한 논피의 유근 저해물질

  • Lim, Chi-Hwan (Department of Bioenvironmental Chemistry, College of Agricultural & Life Sciences, Chungnam National University) ;
  • Kim, Mi-Young (Department of Bioenvironmental Chemistry, College of Agricultural & Life Sciences, Chungnam National University) ;
  • Lee, Jae-Won (Department of Bioenvironmental Chemistry, College of Agricultural & Life Sciences, Chungnam National University) ;
  • Yun, Bong-Sik (Korea Research Institute of Bioscience and Biotechnology) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 임치환 (충남대학교 농업생명과학대학 생물환경화학) ;
  • 김미영 (충남대학교 농업생명과학대학 생물환경화학) ;
  • 이재원 (충남대학교 농업생명과학대학 생물환경화학) ;
  • 윤봉식 (한국생명공학연구원 항산화소재연구실) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2007.12.31
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Abstract

Three phytotoxic compounds were isolated from the culture broth of Chaetomium sp. through silica gel column chromatography and HPLC (RP-18). Their chemical structures were elucidated as chaetoglobosin F, chaetoglobosin C and chaetoglobosin E on the basis of instrumental analyses such as $^1H-NMR,\;^{13}C-NMR$, and HMQC. They inhibited the root growth of barnyard grass with the $IC_{50}$ values of 66, 65, and $67{\mu}g/ml$, respectively.

Chaetomium sp.배양체를 acetone에 침지하여 추출한 후 n-hexane, $CHCl_3$, EtOAc, n-BuOH 및 물로 분획하였다. 이중 EtOAc 분획을 silica gel 및 HPLC(ODS)를 이용하여 3종의 활성물질 A.C-3, A.C-4 및 A.C-5를 분리하였다. 각 화합물의 화학구조를 NMR 및 MS등의 스펙트럼 데이터와 문헌검색을 통하여 chaetoglobosin 유도체인 chaetoglobosin F, chaetoglobosin C 및 chaetoglobosin E로 동정하였다. A.C-3, A.C-4 및 A.C-5의 논피에 대한 유근신장 저해활성 검정 결과, $IC_{50}$값은 각각 66, 65, $67{\mu}g/ml$이었다.

Keywords

References

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