• Journal of the Korean Society of Costume
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• v.50 no.8
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• pp.5-16
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• 2000

This study attempts to examine compound weave among silk fabrics of Cho-Sun Dynasty. These silk fabrics have colorful patterns. They can be classified into two types by their texture structure one is called polychrome woven silk of which structure is plain or twill fabric : another is called satan damask of which structure is stain fabric. Their texture structure consist of warp and weft. In addition. there are other types of warp and weft for pattern structure ; various twill structures were used for patterns. A variety of colorful threads, including gold and silver ones. are used fur patterns in them. The brocaded-type fabric was primarily developed during Won Era of China. It was most developed in Ko-Rye Kingdom. During that era flat strip was used the most but its length was usually not long enough to finish one pattern. However, wrapped thread was increasingly used during the middle and later period of Cho-Sun Dynasty due to the development of weaving technology for gold thread. The brocaded-type fabric was produced in Korea and it was also Imported from China according to some record. There are some domestic empirical documents that show the names of imported Satin Samite, Brocaded satin and these are good evidence to prove the nationwide use of the cloth during Cho-Sun Dynasty. The density ratio of warp and weft is almost same except Samite in the third and Satin with flower motif in the second period is most density among them. C·F of Brocaded satin with Mang motif in the third period values the highest price. Brocaded satin, used with both gold and silver threads, is thickest.

• Biomolecules & Therapeutics
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• v.8 no.2
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• pp.153-159
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• 2000

A novel serine/threonine protein phosphatase with EF-hand motif, which belongs to PPEF family was partially cloned from rat brain cDNA by employing RT-PCR method. The size of the amplified clone was 1.6kbp. The amplified DNA was subcloned into pGEM-T-Easy vector and the resulting plasmid was maned as pGEM-rPPEF2. The nucleuotide sequence is shared by 88% with that of mouse PPEF-2 cDNA, and the deduced amino acid sequence reveal 92% homology with that of mouse PPEF-2 cDNA. The N-terminal region of the cloned rat brain PPEF contains a putative phosphatase catalytic domain (PP domain) and the C-terminal region contains multiple $Ca^{2+}$ binding sites (EF region). The putative catalytic domin (PP) and the EF-hand motif (EF) regions were subcloned into pGEX4T-1 and were overexpressed in E. coli DH5 as glutathione-S-transferase (GST) fusion proteins. Expression of the desired fusion protein was identified by SDS-PAGE and also by immunoblot analysis using monoclonal antibody against GST. The recombinant proteins were purified by glutathione-agarose chromatography. This report is first to demonstrate the cloning of PPEF family from rat brain tissues. The clone reported here would be invaluable for the investigation of the role of this new type-phosphatase in rat brain.

• Journal of Life Science
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• v.15 no.2 s.69
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• pp.192-196
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• 2005

The SNF2/SW12 family comprises proteins from a variety of species with in vivo functions, such as transcriptional regulation, maintenance of chromosome stability during mitosis, and various types of DNA repair. This study was shown the characterization of hrp2+ gene which was isolated by PCR amplification using the conserved domain of SNF2 motifs. Sequence analysis of hrp2+ gene showed striking evolutionary conservation among the SNF2 family of proteins. The transcript of hrp2+ gene was found to be a 4.7 kb as identified by Northern hybridization. To investigate the inducibility of hrp2+ gene, transcript levels were examined after treating the cells to various DNA damaging agents. The transcripts of hrp2+ were induced by UV-irradiation. But the transcripts were not induced by treatment of $ 0.25\%$ Methylmethane sulfonate (MMS). These results implied that the effects of damaging agents are complex and different regulatory pathways exist for the induction of this gene. Hrp2 protein was purified near homogeneity by combination of affinity chromatography. We tested the purified Hrp2 protein for the helicase activity in an oligonucleotide release assay. However we were unable to detect any helicase activity associated with the Hrp2 protein, indicating that the helicase motifs in Hrp2 are merely indicators of a broader DNA-dependent ATPase activity.

• Korean Journal of Human Ecology
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• v.8 no.3
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• pp.565-577
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• 1999

In this world of globalism, achieving the winning position in the competitive international market is essential in the area of fashion industry. For this reason, we should accomplish this task by adapting our own traditional beauty to the modern fashion design through the modernization of the Korean traditional style. Therefore, this study attempted to analyze the fashion design works of the active Korean fashion designer, Sul Yoon-Hyung, focusing on the subject how she incorporated the modern fashion design with the traditional motives. In order to study this research subject, both quantitative and qualitative research methods were used to collect and analyse the textile motives used in the designer's works found from the major fashion magazines, Bazaar, Vogue, Marie Claire, ELLE, WWD, and Fashion Today during the last seven years(1993-1999). The collected research data, 68 works, was counted and analyzed in terms of the categories of Korean traditional motives they were adapted from, the application techniques, and the aesthetic characteristics. As a result of the study, it was found that Sul Yoon-Hyung has adapted different kinds of Korean traditional motives including motives borrowed from the nature such as flowers and animals, geometrical forms, and Chinese letters with different specific symbolic meanings for each motif and many different techniques were utilized to apply these motives. It was apparent to note that the Korean traditional motives were successfully incorporated into her fashion design works expressing the beauty of oriental grace, naturalism, sophistication, and above all, the modern sense.

• Journal of the Korean Society of Clothing and Textiles
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• v.26 no.3_4
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• pp.402-411
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• 2002

The objective of this study was to investigate sensibility images of Korean traditional motifs cognized by college students in U.S.A. The subjects consisted of 217 male and 351 female undergraduate students. The experimental materials used in this study were 48 stimuli and questionnaires, composed of 7-point semantic differential scales of 15 bipolar adjectives. Twelve motifs selected from 3 groups of Korean motifs were used as motif stimuli. Twelve repeated patterns were constructed from them to be applied on a CAD-simulated dress. The data were analyzed by factor analysis, ANOVA, Duncan's multiple range test. The major finding were as follows: 1. Four dimensions were emerged accounting for the dimensional structure of the images of Korean traditional motifs. These dimensions were ‘quality’, ‘simplicity’, ‘cheerfulnees’, and ‘modernity’. Among them, ‘quality’and ‘simplicity’were the major dimensions. 2. Category, interpretation type, composition type, and application object had significant effects on the images of above-mentioned dimensions. The interpretation type had a significant effect on ‘quality’image, the composition type on ‘cheerful’image, and the application object on ‘modernity’image.

• Journal of Microbiology
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• v.45 no.2
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• pp.139-145
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• 2007

The Bombyx mori parvo-like virus (China isolate) DNA polymerase (BmDNV-3 dnapol) gene has been tentatively identified based on the presence of conserved motifs. In the present study, we perform a transcriptional analysis of the BmDNV-3 dnapol gene using the total RNA isolated from BmDNV-3 infected silkworm at different times. Northern blot analysis with a BmDNV-3 dnapol-specific riboprobe showed a major transcript of 3.3 kb. 5'-RACE revealed that the major transcription start point was located 20 nucleotides downstream of the TATA box. In a temporal expression analysis using differential RT-PCR, BmDNV-3 dnapol transcript was detected at low levels at 6 h.p.i., increased from 6 to 36 h.p.i., and remained fairly constant thereafter. Analysis of the predicted DNA polymerase sequence using neighborjoining and protein parsimony algorithms indicated that the predicted 1115-residue polypeptide contained five motifs associated with DNA polymerases synthetic activities and three additional motifs associated with polymerases possessing 3' to 5' exonuclease activity. The molecular phylogenetic analysis of this gene supported the placement of Bombyx mori parvo-like virus in a separate virus family.

• Journal of Life Science
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• v.12 no.2
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• pp.173-181
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• 2002

This study was conducted to do the comparative analysis of mating type locus controlling the direct formation of fruiting body in Schizophyllum commune which is indigenous to North America with that of other identified mating locus. The 3120 bp Y-region nucleotide of A $\alpha$ 3 mating locus activating a developmental pathway in S. commune was determined, and appeared to have about 96% homology to S. commune 1-71 $A\alpha$3 allele indigenous to South America, showing strongly a conservative feature. This nucleotide analysis also showed above 96% homology highly in the seven presumed exons, and about 97% in the acidic rich region (AR), about 99% in homeodomain (H7), about 97% in the basic rich region (BR), about 95% in the serine rich region (Ser) respectively. In the comparative analysis to the translated polypeptide sequence, S. commune A $\alpha$ 3 mating locus containing Y-region also showed about 97% homology to the region of S. commune indigenous to North America, but the identity ratio to Y1 including Y4, Y5, Y6 different allele types was declined to about 41~49%. In the analysis of functional loci controlling mating activity, it is assumed to have a highly conservative feature showing about 98% homology in homeodomain polypeptide. Especially, it is notable that the homology ratio of above 85% in homeodomain motif between mating type alleles was higher than in the AR, BR, Ser showing about 10~50% homology.

• Journal of the Korean Society of Costume
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• v.48
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• pp.5-24
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• 1999

The globalization of Korean beauty comes partly from the succession of korean traditional culture and its modernization will be the one of very important issues in 21st century. This paper was done in order to analyze the process of Korean design development in modern Korean fashion in terms of the succession and modernization of Koreantraditional Beauty and based on this analysis also suggest new possibilities for successful globalization of Korean traditional beauty andbased on this analysis also suggest new possibilities for successful globalization of Korean fashion in 21st century. 227 fashion designs which represent typical elements of korean traditional costume were selected from fashion magazines books SFAA collection books and company brochures published from 1986, to 1997. Content analysis was used with 8 variables including 7 design elements(line shape, color, textile, motif, detail, accessories) and year. Results of analysis showed that the process of Korean Design development in modern Korean fashion has in the use of straight lines and simple silhouettes. Diversity could be found in the application of various kinds of outwear items as well as in the variety of materials and details. Symbolic tradtional motifs and accessories were used to enliven the Korean folklore image, These trends became more salient with the turning point of 1993 when a few designers began to participate in Paris Collection. For successful globalization designers should have more active attitudes toward exploiting the essence of Korean beauty and developing them as modern and global styles.

• Korean Institute of Interior Design Journal
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• v.25 no.4
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• pp.3-12
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• 2016

The purpose of this study was to analyze City squares in Europe according to Biophilic Design elements to apply domestic Indoor Squares in Mixed-use building for relaxation. This study seeks to present a direction for designing an indoor square that uses the aspects of European city square. The following details consist the purpose of the study. First, Perspective from the Biophilic design and analyzing criteria of Europe city square. Second, European city square is analyzed to establish the criteria to be applied to indoor square. Third, based on European city square analysis, the planning direction for domestic indoor square is suggested. This result of paper proceeds as follows. First, it describes the analysis of the Indoor Squares of the domestic Mixed-use building. The results were derived of the fact that not applying the water elements and Facade greening. Second, In Europe Square, It is accounted for the highest proportion (33%) of the spaciousness. Third, Through a comparative analysis domestic indoor square was derived that the higher the need to improve the spaciousness and botanical motif. It is the ultimate goal of this study based on the analysis of the city square to set the criteria for the biophilic design of the indoor square to present the orientation of the indoor square.

• Archives of Pharmacal Research
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• v.22 no.6
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• pp.542-548
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• 1999

The UL47 gene (b 60390-b 60388) located in the unique long region of the human cytomegalovirus (HCMV) AD169 strain genome was analyzed RNA mapping. Northern blot analysis showed that the UL47 gene was expressed at late times after infection (72 h postinfection). The 9.7-kb transcript was expressed in the infected cells but not in phosphonoformate-treated cells at 72 hpi, indicating that the UL47 gene was only expressed at late times after infection. To map the 5'-end and 3'-end of UL47 transcripts, primer at late times after infection. To map the 5'-end and 3'-end of UL47 transcripts, primer extension and RNase protection analysis were performed. Primer extension analysis revealed that the transcription initiation site of UL47 was located in 27 bp downstream (b 60323) of the TATA box motif. The sizes of UL47 ORF (approximately 2.9-kb) and UL48 ORF (approximately 6.7-kb) deduced from computer sequence analysis suggest that the expressed 9.7-kb transcript of UL47 uses the 3'-end polyadenylation signal of Ul48. The result of RNase protection determined that the 3'-end of UL47 RNA utilized the 3'-end polyadenylation signal of UL48, which is located in HCMV genome b 70082.