• 제목/요약/키워드: mortem analysis

검색결과 36건 처리시간 0.019초

차량용 스택 고출력 내구성능 (High Durability of Stack for Automobile)

  • 김영민;이종현;윤종진;조장호
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2007년도 춘계학술대회
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    • pp.557-560
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    • 2007
  • The durability of 80 kW class stack module was tested in hydrogen recirculation and non-recirculation systems with the condition of 300Amps (constant current mode) and hydrogen pulse purging (10 seconds close/0.8 seconds open). A localized membrane failure in the interfacial area between membrane and sub-gasket, carbon corrosion in cathode electrode, and Pt dissolution/extraction have been found through the post mortem analysis such as CV, Impedance, SEM, and so on. The main reason of these mechanisms will be discussed in this study.

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[a] 음성파형과 사상체질과의 상관성연구 - 사상체질음성분석기(四象體質音聲分析機)(PSSC-2004)를 이용하여 (A Study on the Relationship of [a] Sound Wave to Sasang Constitution - by Sasang Constitution Analysed with PSSC-2004)

  • 윤우영;최민기;정운기;이상준;김달래;유준상
    • 사상체질의학회지
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    • 제18권2호
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    • pp.83-95
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    • 2006
  • 1. Objectives and Methods The purpose of this study was to objectify the diagnosis of Sasang constitution. It analyzed the adult male and female voices into 23factors using PSSC-2004. The study was conducted by subjects inputting 2.5-3 sec of [a] in 586 men and women's voices to PSSC-2004. The statistical analyses are applied to three groups: total group, male group, female group. The group with a total of 586 was composed of 155 Soyangin, 230 Taeumin and 201 Soeumin. The male group was composed with 61 Soyangin, 127 Taeumin and 87 Soeumin. The female group was composed with 94 Soyangin, 103 Taeumin and 114 Soeumin. Taeyangin were excluded for a more accurate statistical analysis. 2. Results (I) In total group, Taeumin's peak sum was significantly high compared with the others(P=0.021). It was significant for distinction between Taeumin and Soyangin at the result of post mortem. (2) In total group, the Soyangin's rank6, rank7 and rank8 were significantly high compared with the others (P=0.050)(0.043)(0.034). (3) In total group, the Soyangin's rank9 was significantly high compared with the others(P=0.025). It was significant for distinction between Taeumin and Soyangin at the result of post mortem. (4) In male group, the Soyangin's code3 was significantly high compared with the others(P=0.050). (5) In male group, the Soyangin's code1 was significantly low compared with the others(P=0.004). It was significant for distinction between Taeumin and Soyangin at the result of post mortem. (6) In male group, the Soyangin's rank1, rank2, rank3 and rank4 were significantly high compared with the others(P=0.017)(0.008) (0.005)(0.002). They were significant for distinction between Taeumin and Soyangin at the result of post mortem. (7) In female group, the Taeumin's code1 was significantly low compared with the others(P=0.016). It was significant for distinction between Taeumin and Soeumin at the result of post mortem. 3. Conclusion From above result, there is the possibility of efficiency standard gide for constitutional diagnosis by analyzation of the voices.

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Metabolomic profiling of postmortem aged muscle in Japanese Brown beef cattle revealed an interbreed difference from Japanese Black beef

  • Susumu Muroya;Riko Nomura;Hirotaka Nagai;Koichi Ojima;Kazutsugu Matsukawa
    • Animal Bioscience
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    • 제36권3호
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    • pp.506-520
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    • 2023
  • Objective: Japanese Brown (JBR) cattle, especially the Kochi (Tosa) pedigree (JBRT), is a local breed of moderately marbled beef. Despite the increasing demand, the interbreed differences in muscle metabolites from the highly marbled Japanese Black (JBL) beef remain poorly understood. We aimed to determine flavor-related metabolites and postmortem metabolisms characteristic to JBRT beef in comparison with JBL beef. Methods: Lean portions of the longissimus thoracis (loin) muscle from four JBRT cattle were collected at 0, 1, and 14 d postmortem. The muscle metabolomic profiles were analyzed using capillary electrophoresis time-of-flight mass spectrometry. The difference in post-mortem metabolisms and aged muscle metabolites were analyzed by statistical and bioinformatic analyses between JBRT (n = 12) and JBL cattle (n = 6). Results: A total of 240 metabolite annotations were obtained from the detected signals of the JBRT muscle samples. Principal component analysis separated the beef samples into three different aging point groups. According to metabolite set enrichment analysis, post-mortem metabolic changes were associated with the metabolism of pyrimidine, nicotinate and nicotinamide, purine, pyruvate, thiamine, amino sugar, and fatty acid; citric acid cycle; and pentose phosphate pathway as well as various amino acids and mitochondrial fatty acid metabolism. The aged JBRT beef showed higher ultimate pH and lower lactate content than aged JBL beef, suggesting the lower glycolytic activity in postmortem JBRT muscle. JBRT beef was distinguished from JBL beef by significantly different compounds, including choline, amino acids, uridine monophosphate, inosine 5'-monophosphate, fructose 1,6-diphosphate, and betaine, suggesting interbreed differences in the accumulation of nucleotide monophosphate, glutathione metabolism, and phospholipid metabolism. Conclusion: Glycolysis, purine metabolism, fatty acid catabolism, and protein degradation were the most common pathways in beef during postmortem aging. The differentially expressed metabolites and the relevant metabolisms in JBRT beef may contribute to the development of a characteristic flavor.

Ante-Mortem Diagnosis of an Atypical Mixed Form of Feline Infectious Peritonitis through Pericardial Effusion Analysis in a Cat

  • Junyoung Kim;Yebeen Kim;Jihye Choi;Junghee Yoon
    • 한국임상수의학회지
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    • 제40권4호
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    • pp.308-313
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    • 2023
  • A 1-year-old castrated male Korean Shorthair cat presented with dyspnea, anorexia, lethargy, and seizures. Physical examination revealed salivation, right forelimb hemiparesis, and rapid breathing. No abnormalities were detected on auscultation. Laboratory findings revealed increased levels of bilirubin, aspartate aminotransferase (AST), globulin, glucose, and a decreased albumin-to-globulin (A:G) ratio. Both N-terminal pro-B-type natriuretic peptide (NT-proBNP) and feline serum amyloid A (fSAA) levels were significantly elevated. Thoracic radiography revealed mild cardiomegaly and diffuse increased interstitial infiltration with soft tissue opacity in the periphery of the right caudal pleural space. Echocardiography and lung ultrasonography were performed to investigate the cause of mild cardiomegaly and soft tissue opacity in the pleural space. Echocardiography revealed a mild amount of echogenic pericardial effusion, and lung ultrasonography showed an echogenic soft tissue mass with no blood signal in the right caudal pleural space, suggestive of a granulomatous lesion. After obtaining 5 mL of pericardial fluid through pericardiocentesis, cytology of the pericardial effusion sample revealed marked neutrophils and macrophages with no bacteria. IDEXX feline infectious peritonitis (FIP) virus real-time reverse transcriptase polymerase chain reaction (RT-PCR) confirmed the presence of the FIP virus biotype in the sample. This case presents a rarely reported atypical mixed form of FIP in a cat diagnosed ante-mortem using pericardial effusion analysis. In this case, ultrasound examination played a crucial role in the definitive diagnosis of FIP by PCR biotyping through pericardiocentesis. Ultrasonography can be highly beneficial in guiding the diagnosis and evaluation of cats with suspected FIP.

Occult Gastric Cancer Presenting as Hypoxia from Pulmonary Tumor Thrombotic Microangiopathy

  • Mandaliya, Rohan;Farhat, Salman;Uprety, Dipesh;Balla, Mamtha;Gandhi, Apurva;Goldhahn, Richard;Auerbach, Herbert;Christensen, Chris;Reed, Conrad;Cohen, Sidney
    • Journal of Gastric Cancer
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    • 제14권2호
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    • pp.142-146
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    • 2014
  • Pulmonary tumor thrombotic microangiopathy (PTTM) causing fatal pulmonary hypertension is a rare presentation of malignancy. In general, patients with PTTM rapidly succumb to death due to severe hypoxia. To date, very few cases of PTTM have been reported in the literature; and most of these cases were from gastric cancer and were diagnosed on post mortem autopsy, as it is extremely challenging to make an ante mortem diagnosis. We here report on a case of undiagnosed diffuse gastric cancer, presenting as worsening hypoxia. The clinical, radiographic, and echocardiographic features, and laboratory and pathological results were consistent with PTTM from gastric cancer. The patient was started on anticoagulation therapy, corticosteroids, and high-flow oxygen. However, her hypoxia worsened to the extent that she required ventilator support, and she died soon after intubation due to cardiac arrest. Since diffuse gastric cancer is associated with hereditary diffuse gastric cancer syndrome, cadherin 1 gene mutation analysis was performed to estimate the risk to her daughters. The test came back negative.

비장 조직에서 에탄올 대사체(EtG, EtS)를 검출하는 방법과 유효성 확인 (Method validation of detecting ethanol metabolites (EtG, EtS) in post-mortem spleen)

  • 김수민;조영훈;안성희;이우재;권미아
    • 분석과학
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    • 제34권3호
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    • pp.115-121
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    • 2021
  • 에탄올 대사체인 에틸글루쿠로나이드(ethyl glucuronide, EtG)와 에틸설페이트(ethyl sulfate, EtS)는 에탄올 섭취의 직접적인 지표물질로서 여러 생체 시료에서 법과학적으로 다양하게 연구되고 있다. 부검 변사자에게서 검출된 에탄올이 사망 전 음주에 의한 것인지 사후 부패에 의해 생성된 에탄올인지 판단하기 위해서는 에탄올 대사체를 분석할 필요가 있다. 일반적으로 혈액에서 에탄올과 함께 EtG, EtS를 검출하고 있지만 부패 상황에 따라 혈액을 확보하는 것이 어려우므로 조직 생체시료를 이용해 에탄올 대사체를 검출함으로써 이를 대체해야 한다. 본 연구에서는 조직 중에서 비장을 선택하여 LC-MS/MS를 이용해 EtG, EtS를 검출하였으며 시험법이 적합한 방법인지를 증명하기 위해 밸리데이션을 수행하여 법과학적 감정에 사용할 수 있는 분석법을 완성하고 실제 부검 사례에 적용하였다.

군용 침낭 사용자 요구에 맞춘 설문조사와 결과 분석 (A Study on Result Analysis and Survey of User Requirements for Military Sleeping Bag)

  • 이정문
    • 품질경영학회지
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    • 제42권3호
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    • pp.339-360
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    • 2014
  • Purpose: The objective of this paper was to analyze the requirements of the end-users by class after carrying out a survey on the user requirements for sleeping bags and utilize the result for effective product development. Methods: The test for difference in means by class was conducted through one sample analysis and one-way ANOVA after the survey result has gone through a reliability verification, and an LSD (Least Significant Difference) test was conducted as a post-mortem method. Results: A significant result was derived from the survey on the user requirements for sleeping bags by method of use, use of combination of sleeping bag components or a sleeping bag alone. Conclusion: The significant result derived from the survey of this paper on the user requirements for sleeping bags can be utilized for effective development of sleeping bags.

자동 개인식별을 위한 안면삼각법과 히스토그램분석 (Facial Triangle and Histogram Analysis for Automatic Super-impose Individual Recognition)

  • 이진행;송현교;강민구
    • 한국정보통신학회논문지
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    • 제3권2호
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    • pp.321-327
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    • 1999
  • 본 연구는 스캐너로 입력한 사진을 법의학을 응용한 안면삼각법(facial triangle)과 사진분석법을 이용하여 수직각, 수평각 등을 측정한 각도에서 CCD 카메라로 두개골 영상을 입력받아 중첩시키기 위한 슈퍼임포즈 개인식별 영상시스템의 성능향상과 다양한 영상처리 응용 프로그램을 활용함으로서 자동식별을 위한 개인식별 능력을 향상하였다.

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[i]음성파형과 사상체질과의 상관성연구 - 사상체질음성분석기(四象體質音聲分析機)(PSSC-2004)를 이용하여 (A Study on the Relationship of [i] Sound Wave to Sasang Constitution - by Sasang Constitution Analysed with PSSC-2004)

  • 송학수;정운기;최민기;김종채;김달래;유준상
    • 사상체질의학회지
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    • 제18권2호
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    • pp.68-82
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    • 2006
  • 1. Objectives and Methods The purpose of this study was to objectify the diagnosis of Sasang constitution. It was analyzed the adult men and women's [i] sound into 23 factors with PSSC-2004. The study was conducted by subjects inputting 2.5-3 sec of [i] of 586 adult men and women's voices to PSSC-2004. The statistical analysis are applied to three groups : total group, male group, female group. The group of total 586 was composed with 155 Soyangin, 230 Taeumin and 201 Soeumin. The male group was composed with 61 Soyangin, 127 Taeumin and 87 Soeumin. The female group was composed with 94 Soyangin, 103 Taeumin and 114 Soeumin. 2. Results (1) In total group, the Soyangin's code3 was significantly low compared with the others(P=0.011). In total group, the Taeumin's code2 and the Soyangin's code1 were significantly high compared with the others(p=0.007)(P=0.030). (2) In total group, the Soyangin's peak, 50 up in peak and under 3 in peak were significantly low compared with the others(P=0.003) (P=0.005)(P=0.023). (3) In total group, the Taeumin's rank7, rank8, rank9 and rank10 were significantly high compared with the others (P=0.013)(P=0.015) (P=0.016)(P=0.003). (4) In male group, the Soeumin's code3 was significantly high compared with the others(P=0.002). (5) In male group, the Soeumin's peak sum was significantly high compared with the others(P=0.009). It was significant for distinction between Soeumin and Soyangin at the result of post mortem. In male group, the Soeumin's 50 up in peak, 50 down in peak were significantly high compared with the others(P=0.049)(p=0.037). In male group, the Soeumin's under 3 in peak was significantly high compared with the others(P=0.016). It was significant for distinction between Soeumin and Soyangin at the result of post mortem. (6) In male group, the Soeumin's rank 2, rank 3 and rank 4 were significantly low compared with the others(P=0.011) (0.011)(0.024). (7) In female group, the Taeumin's code2 was significantly high compared with the others(P=0.023). In female group, the Soyangin's code1 was significantly low compared with the others (P=0.046). In female group, Soyangin's code-1 was significantly high compared with the others(P=0.024). It was significant for distinction between Taeumin and Soyangin at the result of post mortem. (8) In female group, the Taeumin's peak sum was significantly high compared with the others(P=0.024). It was significant for distinction between Taeumin and Soyangin at the result of post mortem. In female group, the Taeumin's 50 up in peak was significantly high compared with the others(P=0.012). (9) In female group, the Taeumin's rank 7, rank 8, rank 9 and rank 10 were significantly high compared with the others (P=0.009) (P=0.013)(P=0.016)(P=0.023). 3. Conclusion From above result, there is the possibility of efficiency standardguide for constitutional diagnosis by analyzation of the voices.

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말쥐치육의 사후경과에 따른 단백질조성의 변화 (Change in Protein Composition of Filefish Muscle during Post-Mortem Lapse)

  • 변재흥;남택정
    • 한국수산과학회지
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    • 제14권1호
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    • pp.15-23
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    • 1981
  • 말쥐치 등육 골낙근을 $0^{\circ}C$로 유지하면서 사후경과중 선도의 변화에 따라 단백질의 조성과 단백질의 아미노산 조성 및 유리아미노산의 조성 등을 분석 검토하였다. 말쥐치의 육은 약 $20\%$의 단백질을 함유하고 있었으며, 이 단백질은 근원질단백질 $31\%$, 근원섬유단백질 $55\%$, 세포내잔사단백질 $10\%$, 그리고 기질단백질 $4\%$로 구성되어 있었다. 근원질단백질과 근원섬유단백질은 사후선도의 변화와 더불어 감소하여 갔고, 세포내잔사단백질은 상대적으로 증가하는 관계를 보였다. 근원섬유단백질과 세포내잔사단백질은 전기영동과 densitometry에 의하여 분석한 결과, 근원섬유단백질의 약 $70\%$가 액틴과 미요신, 약 $20\%$가 조절단백질, 그리고 $10\%$전후의 미지단백질로 구성되어 있었다. 세포내잔사단백질은 그 대부분이 근원섬유단백질을 구성하는 단백질로 이루어져 있었다. 한편, 사후경과와 더불어 근원섬유단백질을 구성하는 단백질중 트로포닌-T, 트로포닌-C 및 미요신의 light chain 2는 각각 감소하는 추세를 보였다. 죽인 직후의 말쥐치 육단백질의 아미노산 조성은 다른 어육의 그것과 비슷하였으나, 트?토판과 함황아미노산은 보다 부족하였다. 휘발성 염기질소양에비추어 부패초기에 해당하는 사후 18일이 경과했을 때는 프롤린과 시스테인이 두드러지게 줄었으며, 로이신과 이소로이신 및 페닐알라닌은 조금 증가하였다. 말쥐치육의 유리아미노산중에는 타우린이 특히 많이 함유되어 있었으며, 알라닌과 글리신 및 리신도 비교적 많았다. 사후 18일이 경과했을 때는 타우린, 히스티딘, 발린 및 메치오닌은 증가하였으나 리신, 아르기닌, 아스파르트산, 스레오닌, 세린, 로이신 및 이소로이신은 반대로 감소하였다.

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