• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.213-225
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• 2016

To reduce attrition in drug development, it is crucial to consider the development and implementation of translational phenotypic assays as well as decipher diverse molecular mechanisms of action for new molecular entities. High-throughput fluorescence and confocal microscopes with advanced analysis software have simplified the simultaneous identification and quantification of various cellular processes through what is now referred to as high-content screening (HCS). HCS permits automated identification of modifiers of accessible and biologically relevant targets and can thus be used to detect gene interactions or identify toxic pathways of drug candidates to improve drug discovery and development processes. In this review, we summarize several HCS-compatible, biochemical, and molecular biology-driven assays, including immunohistochemistry, RNAi, reporter gene assay, CRISPR-Cas9 system, and protein-protein interactions to assess a variety of cellular processes, including proliferation, morphological changes, protein expression, localization, post-translational modifications, and protein-protein interactions. These cell-based assay methods can be applied to not only 2D cell culture but also 3D cell culture systems in a high-throughput manner.

• Elastomers and Composites
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• v.38 no.2
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• pp.128-138
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• 2003

It goes to be serious with environmental pollution because of great number of waste tires scrapped each you. That is why there are lots of studies for efficient recycle. We tried to reduce particle size of the waste tire powder using a new technology of self-designed grinding machine (SDGM) and ultrasonic treatment. The purpose of this study is to improve the physical properties of reduced waste tire powder. We investigated the fine powder by particle size distribution(PSD) analysis. And also we examined the physical and mechanical properties and cross-link density at various particle sire. Also we carried out morphological studies after making the products by SEM.

• Journal of Ocean Engineering and Technology
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• v.30 no.6
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• pp.484-497
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• 2016

In order to understand the characteristics of the topography change in front of an impermeable breakwater, a coupled model for a two-way analysis of the existing LES-WASS-2D and newly developed morphodynamic model was suggested. A comparison to existing experimental results revealed that the results computed using the 2-D hydro-morphodynamic model were in good agreement with the experimental results for the wave form, pore water pressure in the seabed, and topographical change in front of a submerged breakwater. It was shown that the two-way model suggested in this study is applicable to a morphological change in the seabed around a submerged breakwater. Then, using the numerical results, the topographical changes in front of an impermeable submerged breakwater were examined in relation to partial standing waves. Moreover, the characteristics of the local scour depths in front of them are also discussed in relation to incident wave conditions, sediment qualities, and submerged breakwater shapes.

• Macromolecular Research
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• v.17 no.8
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• pp.597-602
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• 2009

Although the existing design principle of full-sequence ionic complement is convenient for the development of peptides, it greatly constrains the exploration of peptides with other possible assembly mechanisms and different yet essential functions. Herein, a novel designed half-sequence ionic complementary peptide (referred to as P9), AC-Pro-Ser-Phe-Asn-Phe-Lys-Phe-Glu-Pro-$NH_2$, is reported. When transferred from pure water to sodium chloride solution, P9 underwent a dramatic morphological transformation from globular aggregations to nanofibers. Moreover, the rheological experiment showed that the P9 could form a hydrogel with a storage modulus of about 30 Pa even at very low peptide concentration (0.5% (wt/vol)). The P9 hydrogel formed in salt solution could recover in a period of about 1,800 sec, which is faster than that in the pure water. The data suggestcd that the half-sequence, ionic complementary peptide might be worthy of further research for its special properties.

• Journal of Fashion Business
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• v.24 no.6
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• pp.46-62
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• 2020

This study analyzed contextual expression types in modern fashion show space derived from the architecture based on Pepper's Contextualism. Pepper's Contextualism theory was applied to fashion showcases of the world's four largest collections from 2010 to 2020 by analyzing contextual expression in architecture, resulting in six contextual expression types. The first type was natural environmental contextual expression in which a modern fashion was fused with the natural environment using the natural environment's context. The second type was a temporal contextual expression expressed by blending the theme of a fashion show and an artificial environment that could express past, present, and future issues. The third type was a spatial contextual expression that extended in a flat or three-dimensional direction. This expression could connect with customers in the field. The fourth type was an associative contextual expression that could formally present the theme of a fashion show or a designer's inspiration by borrowing a specific place. The fifth type was an ideological contextual expression that could express a designer's concept or value through a fashion show's design. The sixth type was a contextual communication expression that could allow viewers to immerse themselves in the space of a fashion show venue mentally through a morphological or virtual technique and experience a fashion show.

• The Research Journal of the Costume Culture
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• v.29 no.4
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• pp.603-615
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• 2021

The aim of this study was to analyze the semantic network structure of keywords and the visual composition of images extracted from Instagram in relation to the multi-persona phenomenon with in fashion imagery, which has recently been attracting attention. To this end, the concept of a 'secondary character', which forms a separate identity from a 'main character' on various social media platforms as well as on the airwaves, was considered as the spread of multi-persona and #SecondaryCharacter on Instagram was investigated. 3,801 keywords were collected after crawling the data using Python and morphological analysis was undertaken using KoNLP. The semantic network structure was then examined by conducting a CONCOR analysis using UCINET and Netdraw to determine the top 50 keywords. The results were then classified into a total of 6 clusters. In accordance with the meaning and context of the keywords included in each cluster, group names were assigned : virtual characters, relationship with the main character, hobbies, daily record, N-job person, media and marketing. Image analysis considered the technical, compositional, and social styles of the media based on Gillian Rose's visual analysis method. The results determined that Instagram uses fashion images that virtualize one's face to produce multi-persona representation s that show various occupations, describe different types of hobbies, and depict situations pertaining to various social roles.

• Animal Bioscience
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• v.35 no.6
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• pp.938-947
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• 2022

Objective: The beneficial effects of glycine were tested in piglets with diquat-induced hepatic injury. Methods: Thirty-two piglets were assigned by a 2×2 factorial experimental design including glycine supplementation and diquat challenge. After 3 weeks of feeding with a basic diet or a 1% glycine supplemented diet, piglets were challenged with diquat or saline. After 1 week later, the piglets were slaughtered and samples were collected. Results: Our results indicated that glycine alleviated diquat induced morphological hepatic injury, decreased the activities of plasma alanine aminotransferase, aspartate aminotransferase and glutamyl transpeptidase in the piglets under diquat challenge, and increased total antioxidant capacity and antioxidative enzyme activity significantly. Adding glycine enhanced the concentrations of hepatic adenosine triphosphate and adenosine diphosphate. Transmission electron microscope observation showed that diquat induced clear hepatocytes ferroptosis and its effect could be alleviated by glycine to a certain degree. Moreover, glycine significantly affected mRNA and protein expression of ferroptosis-related signals in the liver. Conclusion: These results demonstrated that glycine attenuated liver damage via inhibiting ferroptosis.

• Physical Therapy Rehabilitation Science
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• v.12 no.1
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• pp.33-42
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• 2023

Objective: This study aims to analyze the effect of regular exercise through the combined walking-oriented aerobic and resistance exercises using EX1 in young adults. Design: Experimental one group pre and post test Methods: Participants comprised17 healthy young adults. All subjects performed a combined exercise program for 10 times using EX1. We measured quadriceps muscle thickness using ultrasound. Additionally, the hand grip strength test, and sit and reach test were performed before and after the exercise. Through paired t-test, we investigated whether there was a statistically significant difference in the measurement results after exercise program. Results: The rectus femoris muscle contraction ratio showed significant difference after exercise(P< 0.01). In the sit and reach test, flexibility showed significant difference after exercise(P < 0.01). The hand grip strength test also showed significant difference after exercise(P < 0.05). Conclusions: Healthy young adults can effectively perform various exercises commonly performed in daily life using EX1.

• Journal of the Society of Naval Architects of Korea
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• v.60 no.4
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• pp.240-247
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• 2023

In this study, morphological and hydrodynamic characteristics of the non-axisymmetric supercavity generated behind a disk-shaped cavitator were examined. By extending the previous study on axisymmetric supercavitating flow based on a boundary element method, hydrodynamic forces acting under the angle of attack condition of 0 to 30 ° and shape characteristics of the supercavity were analyzed. The results revealed that increasing the angle of attack by 30 ° reduced the length and width of the cavity by about 15% and the volume by about 40 %. An empirical formula that can quantitatively estimate the geometrical characteristics and change of the cavity was derived. It is expected that this method can be used to evaluate the shape information and force characteristics of the supercavity for the control of the vehicle in a very short time compared to the viscous analysis in the initial design stage of the supercavity underwater vehicle.

• Design & Manufacturing
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• v.18 no.3
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• pp.30-36
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• 2024

As the demand for biomaterials and medical devices increases due to advancements in medical technology and the rising average lifespan of the population, the importance of surface treatment technology for biometallic materials used in orthopedic implants is highlighted. Achieving stable mechanical attachment between the implant and human bone, specifically bone cell adhesion, is crucial. Without proper adhesion, issues such as inflammation and reduced load-bearing capacity can occur, leading to the need for implant reimplantation. Therefore, this paper focuses on creating a micro-groove pattern using a pulsed nanosecond laser on the surface of a titanium alloy (Ti6Al4V), a biometallic material, to promote cell adhesion. To evaluate the effectiveness of the pattern in enhancing cell adhesion, MG-63 osteoblasts were cultured on the micro-groove patterned surface, and their adhesion and morphological changes were analyzed. This study confirms the potential of laser processing as a surface treatment method for biometallic materials.