• Journal of Mushroom
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• v.6 no.2
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• pp.43-46
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• 2008

This study was carried out to investigate the utilization of platanus for Pleurotus ostreatus culture medium. The results are as follows ; The growth of mycelial was the highest in the mixture medium of platanus sawdust 90% with rice bran 10% as of 12.8cm after 30days culture. The mycelia density of most of media were high exception of mixture medium, platanus tree sawdust 60% with rice bran 40%. The total nitrogen, phosphate, and potassium contents in all mushroom media were tended to decrease after oyster mushroom growth. In platanus sawdust 90% with rice bran 10%, total nitrogen, potassium, calcium and magnesium content of fruit body were higher than other treatments, but phosphate content was the highest in Douglas-fir sawdust 80% with rice bran 20% treatment as of 0.92%. The mycelial culture period was ranged 26~37 days according to different media, the mushroom yield of culture medium with platanus tree sawdust 80% with rice bran 20% was increased 18% compare to control Douglas-fir sawdust 80% with rice bran 20%.

• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.219-223
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• 1998

Anthers contanining uninucleate microspores of eight cultivars of pepper were cultured on MS medium supplemented with 0.004mg/L 2,4-D and 0.1mg/L kinetin, 3% sucrose and 0.2% Gelrite, kept at $35^{\circ}C$ for 24 h, and then cultured at $25^{\circ}C$ with a photoperiod of 16 h daylight for 40 days. Frequency of embryoid and callus formation was varied with cultivars. Embryoid formation was found in Cheongyang and Fushimi Amanaga, while callus formation was in California Wonder, Fushimi Amanaga and Geoseong. In anther culture medium supplemented with 1% activated charcoal, embryoid formation was found with 0.5% of frequency only in Cheongyang, while no callus formation was found. In 1/2MS medium, frequency of embryoid formation in Shishitou, Yatsufusa and Taka no Tsume was 1.2%, 0.4% and 0.4%, respectively. On the other hand, in 1/2 B5 medium, frequency of callus formation in Yatsufusa and Taka no Tsume was 2.8% and 2.7%, respectively. Embryoids transferred to hormone-free MS medium were developed to plantlets and acclimatized. The number of chromosomes in the root tip cells of the haploid plant was 2n=$\times$=12 in cv. Cheongyang.

• Journal of Bio-Environment Control
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• v.22 no.1
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• pp.1-6
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• 2013

This study was conducted to examine the effects of coir, peatmoss, and red clay (20%) + peatmoss (80%) media on quality and drainage solution in hydroponics of Solanum lycopersicum 'Mascara'. The tomato seedlings were planted in media on 29 April, and supplied with Yamazaki's tomato solution of EC $2.0dS{\cdot}m^{-1}$ and pH 6.5. Tomato fruits were harvested from 13 June to 24 August. Drainage amount decresed when solar radiation and air temperature were high. However, drainage amount were not different among coir, peatmoss, and red clay + peatmoss media. The EC of drainage in red clay + peatmoss medium was higher than that in other media during the cultivation period. Also, soild state and available moisture content was more in red clay + peatmoss mediun than in coir or peatmoss media. The soluble solids of tomato fruits increased by 10~17% at $5.5^{\circ}Brix$ in red clay+peatmoss medium compared with $5.0{\sim}4.7^{\circ}Brix$ in coir or peatmoss media. Also, the acidity of fruits was the highest to 0.66% in red clay + peatmoss medium than the others. The total yield of fruits in red clay + peatmoss medium increased significantly by 9.1% at 8,428 kg than at 7,725 kg in peatmoss medium, and ratio of marketable yield was higher than the other media. Therefore, red clay (20%) + peat moss (80%) medium is recommend for growth and quality of fruits in hydroponics of Solanum lycopersicum 'Mascara'.

• Journal of Mushroom
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• v.16 no.2
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• pp.96-102
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• 2018

Sparassis latifolia is one of the most expensive mushrooms in Korean market owing to its high ${\beta}$-glucan content and immunoactivity. However, because of the long cultivation period and high contamination rates, it has low production efficiency. Therefore, we first need to establish the optimum conditions for liquid spawn production to increase its production efficiency. As a result of experiments, molasses culture medium was selected for mycelial growth. Also, the optimum sugar content for molasses and amount of aeration used were approximately 8 Brix% and 0.3~0.6 vvm, respectively. Mycelial dry weight increases, while the medium decreases, as the incubation period increases. Therefore, to achieve maximum production efficiency, the incubation period of 9 to 11 days is appropriate.

• Food Science and Preservation
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• v.10 no.4
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• pp.493-497
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• 2003

In order to prevent a markdown and improve a value added of apricot, this study was conducted to research the optimum condition for manufacture of wine using apricot. On fermentation of aprirot wine, the best yeasts were Saccharomyces cerevisiae JBS 15 and JBS 30, and optimum composition or medium was apricot juice of 50％, sugar of 24 。brix, (NH$_4$)$_2$SO$_4$ of 0.2％, Na$_2$SO$_3$ of 0.02%. The content of alcohol in this medium after fermentation of 10 days at 25$^{\circ}C$ was 12.3∼12.5%. sensory evaluation showed that color, taste and odor or apricot wine were good, and saccharomyces cerevisiae JBS 15 was not difference JBS 30.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2003.04a
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• pp.104-111
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• 2003

우리나라의 가지는 2001넌 866ha에서 재배하여 28.4천톤이 생산되었다(농촌진흥청. 2001). 또한 1994년부터 일본에 수출하기 시작하여 2001년에는 1,948톤에 3,541천불을 기록하고 있다. 이러한 물량은 일본 전체 유통물량 448천톤의 0.5%에 불과하여 앞으로 일본수출 물량을 늘려나갈 수 있는 여지가 많다고 할 수 있다. 그러나 일본에서 요구하는 안전하고 고품질의 가지를 저비용으로 생산하기 위해서는 시설 내에서 수경재배, 격리상 재배가 필요하다고 생각한다. (중략)

• Korean Journal of Plant Resources
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• v.20 no.3
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• pp.251-254
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• 2007

This study was conducted to investigate the optimal plant growth regulator level for the shoot formation of Lycium chinense Mill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants were cultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regenerated through in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shoot formation had developed from the leaf explants. The shoot formation rate of 'Jangmyeong' was highest followed by 'Myeongan', ;Cheondae', and 'Bullo'. The use of 0.2mg/L BA was critical for enhanced production of shoot formation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots were developed and successfully acclimatized to greenhouse.

• The Korean Journal of Pain
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• v.35 no.2
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• pp.160-172
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• 2022

Background: The authors established an in vitro model of diabetic neuropathy based on the culture system of primary neurons and Schwann cells (SCs) to mimic similar symptoms observed in in vivo models of this complication, such as impaired neurite extension and impaired myelination. The model was then utilized to investigate the effects of insulin on enhancing neurite extension and myelination of diabetic neurons. Methods: SCs and primary neurons were cultured under conditions mimicking hyperglycemia prepared by adding glucose to the basal culture medium. In a single culture, the proliferation and maturation of SCs and the neurite extension of neurons were evaluated. In a co-culture, the percentage of myelination of diabetic neurons was investigated. Insulin at different concentrations was supplemented to culture media to examine its effects on neurite extension and myelination. Results: The cells showed similar symptoms observed in in vivo models of this complication. In a single culture, hyperglycemia attenuated the proliferation and maturation of SCs, induced apoptosis, and impaired neurite extension of both sensory and motor neurons. In a co-culture of SCs and neurons, the percentage of myelinated neurites in the hyperglycemia-treated group was significantly lower than that in the control group. This impaired neurite extension and myelination was reversed by the introduction of insulin to the hyperglycemic culture media. Conclusions: Insulin may be a potential candidate for improving diabetic neuropathy. Insulin can function as a neurotrophic factor to support both neurons and SCs. Further research is needed to discover the potential of insulin in improving diabetic neuropathy.

• Journal of Mushroom
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• v.18 no.4
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• pp.317-322
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• 2020

This experiment was conducted to solve the failure of fruiting body production in the bottle cultivation of the oyster mushroom cultivar 'Heuktari'. The effects of incubation temperature on primordium formation and fruiting body yield of the oyster mushroom cultivar 'Heuktari' were investigated. The proper temperature for mycelium growth of 'Heuktari' on potato dextrose agar (PDA) medium is 23-26℃. The mycelial growth of 'Heuktari' was faster than that of Chunchu 2ho. During mycelial culture in sawdust medium, the temperature of the medium in the bottle initially increased, reached the highest point in the middle of the culture, and then decreased. The higher the set temperature, the shorter the incubation period. When the incubation temperatures were 20℃ and 24℃, respectively, the undeveloped primordium formation rates were low (1.8% and 4.2%, respectively). However, the rate of undeveloped primordium formation increased, and the yield decreased at incubation temperatures of 16℃ and 28℃. Mushroom farms that set incubation temperatures to 18℃ and maintained the medium temperature at less than 28℃ showed undeveloped primordium formation rates ranging between 0.3-0.8%. The rate of undeveloped primordium formation increased and the yield decreased in the farms with high incubation temperatures (above 28℃). We found that in order to reduce undeveloped primordium formation, the air inside the incubation room should be circulated continuously so that the temperature of the medium does not rise above 28℃, and dense incubation conditions should be avoided.

• Journal of Mushroom
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• v.21 no.3
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• pp.160-166
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• 2023

The effects of punching treatment on mycelial culture and fruiting body productivity were investigated in a new Lentinula edodes cultivar, "Jadam", in sawdust medium for the stable production of oak mushroom. As the punching volume and number increased, the weight loss rate and color difference increased and the L value decreased. After spawn inoculation, the sawdust medium temperature and CO₂ concentration reached their highest values at 33 and 19 days of incubation, respectively. The O₂ concentration showed the lowest value on the 14^th day of incubation, which was the opposite pattern to the CO₂ concentration. As the punching volume and the number increased, the medium temperature and O₂ concentration increased, and the CO₂ concentration decreased. Higher punching volumes and numbers resulted in higher temperatures and lower CO₂ concentrations. The best fruiting body yield was 5 × 70 mm - 30 (punching diameter × depth - number), and the total yield after three cycles was 644.7 g.