Plant Regeneration through Leaf Explant Culture of Boxthorn (Lycium chinense Mill.)

  • Ham, In-Ki (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Park, Sang-Kyu (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Lee, Bong-Chun (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Lee, Mi-Ae (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Kwon, Kyeong-Hak (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Lee, Eun-Mo (Chungcheongnam-do Agricultural Research & Extension Services) ;
  • Jo, Man-Hyun (Chungcheongnam-do Agricultural Research & Extension Services)
  • Published : 2007.06.30

Abstract

This study was conducted to investigate the optimal plant growth regulator level for the shoot formation of Lycium chinense Mill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants were cultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regenerated through in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shoot formation had developed from the leaf explants. The shoot formation rate of 'Jangmyeong' was highest followed by 'Myeongan', ;Cheondae', and 'Bullo'. The use of 0.2mg/L BA was critical for enhanced production of shoot formation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots were developed and successfully acclimatized to greenhouse.

Keywords

References

  1. Chungcheongnam-do Agricultural Research & Extension Services. 1997. Research report. pp. 773-776
  2. Chungcheongnam-do Agricultural Research & Extension Services. 2000a. Research report. pp. 417-424
  3. Chungcheongnam-do Agricultural Research & Extension Services. 2000b. Research report. pp. 425-432
  4. Chungcheongnam-do Agricultural Research & Extension Services. 2004. Research report. pp. 373-380
  5. Hotta, M., K. Ogata, A. Nitta, K. Hosikawa, M. Yanagi and K. Yamazaki. 1989. Sekai yuhyou-shokubutu jiten (useful plant of the world). Heibonsha, Tokyo. pp. 640-641
  6. Jo, M.H., I.K. Ham, B.C. Lee, J.W. Kim, W.S. Lee, S.Y. Kwon, H.S. Lee and S.S. Kwak. 2004. High frequency shoot formation and plant regeneration from cotyledonary hypocotyl explants of boxthorn (Lycium chinense Mill.) seedlings. Korean J. Plant Biotechnol. 31: 203-207 https://doi.org/10.5010/JPB.2004.31.3.203
  7. Kim, D.C., H.J. Chung, B.H. Min and D.C. Yang. 2001. Plant regeneration from leaf and internode segment cultures of boxthorn (Lycium chinense Mill.). Korean J. Plant Biotechnol. 28: 329-333
  8. Kim S.M. 1997. Agronomic characteristics and changes in chemical components as affected by harvest time in Lycium chinense Mill. Department of Agronomy Graduate School, Dankook University Ph.D. Thesis
  9. Kurokawa S. 1962. General pharmacological studies of the water and various organic solvent soluble components derived from Lycium chinense. Shikoku I gkuzasshi. 18: 127-136
  10. Lee, B.C., S.D. Kim, T.S. Yun, B.H. Lee, S.W. Ra, Y.C. Park, I.S. Woo, D.H. Kim and T.S. Jeon. 2000. Production of new cultivar in boxthorn (Lycium chinense Mill.). Research report of Chungcheongnam-do Agricultural Research & Extension Services, Yeasan, Korea. pp. 417-424
  11. Lee, B.C., J.S. Park, T.S. Kwak and C.S. Moon. 1998. Variation of chemical properties in collected boxthorn varieties. Korean J. Breed. 30: 267-272
  12. Lee, M.S., D.C. Kim, J.H. Kim and W.J. Lim. 1984. Studies on the tissue culture of Lycium chinense Mill. Bul. Agr. Wonkwang Univ. 7: 261-275
  13. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  14. Park, Y.G., B.W. Kim, M. S. Choi and K.S. Noh. 1993. In vitro organogenesis from leaf callus of Lycium chinense Mill. Korean J. Plant Tissue Culture 20: 85-89
  15. Tang, W. and G. Eisenbrand. 1986. Chinense drugs of plant origin. Springer-Verlag. pp. 633-638