• 제목/요약/키워드: mediator-less

검색결과 58건 처리시간 0.022초

공공건설공사 발주방식에 따른 비용성과 실증비교 - 설계변경에 대한 낙찰률 매개효과를 중심으로 - (Cost Performance Comparison of Project Delivery Methods in Public Sector - Focusing on Mediator Effect of Biddropping on Change Orders -)

  • 문효수;이현수;박문서;이보경;주선우;손보식
    • 한국건설관리학회논문집
    • /
    • 제16권5호
    • /
    • pp.86-96
    • /
    • 2015
  • 기존연구에서 Design-Build(이하 "DB")가 Design-Bid-Build(이하 "DBB")발주방식보다 비용측면에서 우월하다고 하는 이유는 설계변경이 적기 때문이다. 그러나 만약 DBB가 낙찰률이 낮아 이것을 만회하기 위해 설계변경을 발생시켰다면, DB가 설계변경이 적기 때문에 우월하다는 것은 잘못된 판단이다. 따라서 이러한 판단의 오류를 확인하기 위해 발주방식과 설계변경, 혹은 낙찰률과 설계변경의 영향관계만 분석하던 두 종류의 연구들을 종합적으로 분석하는 것을 시도했다. 또한 경로분석기법을 통해 프로젝트 타입 별로 발주방식과 낙찰률이 설계변경에 미치는 효과크기를 동시에 비교함으로써 낙찰률의 매개효과를 검증하였다. 그 결과 특정 프로젝트 타입에서는 발주방식과 설계변경 사이에 낙찰률이 개입하여 매개효과를 발생시키고 있으므로, DB가 DBB보다 설계변경이 적기 때문에 우월하다고 판단할 수 없음을 검증하였다. 이로써 발주방식이 낙찰률을 통해 설계변경에 작용하는 원리를 밝히고, 낙찰률의 정체성을 기존 연구와는 다른 관점에서 규명하였다. 이를 바탕으로 프로젝트 특성에 따른 매개효과를 고려한 발주방식 선정과 성과측정에 도움을 줄 수 있을 것이라고 기대한다.

A Microbial Fuel Cell Type Lactate Biosensor Using a Metal-Reducing Bacterium, Shewanella putrefaciens

  • KIM, HYUNG JOO;MOON SIK HYUN;IN SEOP CHANG;BYUNG HONG KIM
    • Journal of Microbiology and Biotechnology
    • /
    • 제9권3호
    • /
    • pp.365-367
    • /
    • 1999
  • A fuel cell type biosensor for lactate was developed using a metal-reducing bacterium, Shewanella putrefaciens IR-1. Under the operational conditions, the bacterial cell suspension generated the current without an electrochemical mediator in the presence of lactate. The current was proportional to the lactate concentration up to 30 mM.

  • PDF

Microbiology of a Mediator-less Microbial Fuel Cell

  • Kim, Byung-Hong;Chang, In-Seop;Park, Hyung-Soo;Lee, Ji-Young;Thu Nguyet Phung
    • 한국미생물생명공학회:학술대회논문집
    • /
    • 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
    • /
    • pp.75-79
    • /
    • 2004
  • PDF

Disposable Strip-Type Biosensors for Amperometric Determination of Galactose

  • Gwon, Kihak;Lee, Seonhwa;Nam, Hakhyun;Shin, Jae Ho
    • Journal of Electrochemical Science and Technology
    • /
    • 제11권3호
    • /
    • pp.310-317
    • /
    • 2020
  • A development of disposable strip-type galactose sensor for point-of-care testing (POCT) was studied, which was constructed using screen-printed carbon electrodes. Galactose levels were determined by the redox reaction of galactose oxidase in the presence of potassium ferricyanide as an electron transfer mediator in a small sample volume (i.e., less than 1 µL). The optimal performance of biosensor was systematically designated by varying applied potential, operating pH, mediator concentration, and amount of enzyme on the electrode. The sensor system was identified as a highly active for the galactose measurement in terms of the sensitivity (slope = 4.76 ± 0.05 nA/µM) with high sensor-to-sensor reproducibility, the linearity (R2 = 0.9915 in galactose concentration range from 0 to 400 µM), and response time (t95% = <17 s). A lower applied potential (i.e., 0.25 V vs. Ag/AgCl) allowed to minimize interference from readily oxidizable metabolites such as ascorbic acid, acetaminophen, uric acid, and acetoacetic acid. The proposed galactose sensor represents a promising system with advantage for use in POCT.

게임 이용자의 동기부여와 몰입이 충성도에 미치는 영향 (The Influence of Game Players' Motivation and Flow on Loyalty)

  • 이상종;변현수
    • 디지털융복합연구
    • /
    • 제17권5호
    • /
    • pp.207-215
    • /
    • 2019
  • 본 연구에서는 비디오게임 이용자가 인식하는 내적 및 외적 동기부여와 몰입이 게임 충성도에 미치는 영향을 알아보고자 수행되었다. 또한 이러한 관계에서 몰입이 실제로 매개역할을 수행하는지도 확인해 보도록 하였다. 기존 연구를 토대로 설문지를 작성하여 분석에 필요한 설문결과를 취합하여 이를 기반으로 연구결과를 확인해 보았다. 실증분석 결과 게임 이용자의 내적 동기부여 및 외적 동기부여는 게임 충성도에 유의한 영향을 미치고 있는 것으로 나타났다. 여기에 몰입이라는 매개변수가 매개효과를 발휘하고 있음도 확인하였다. 다만 외적 동기부여는 내적 동기부여보다 영향력이 높지 않음을 아울러 확인할 수 있었다. 향후 가상현실 게임이나 기능성 게임 등의 발전에 본 연구에서 제시한 사용자의 내재적 가치변화에 관한 부분을 적용해 볼 수 있을 것이다.

미생물 연료전지에서 Fe[III] 환원 미생물 Geobacter sulfurreducens를 이용한 전기 생산 (Electricity Production from Fe[III]-reducing Bacterium Geobacter sulfurreducens in Microbial Fuel Cell)

  • 이유진;오유관;김미선
    • 한국수소및신에너지학회논문집
    • /
    • 제19권6호
    • /
    • pp.498-504
    • /
    • 2008
  • Metal-reducing bacterium, Geobacter sulfurreducens is available for mediator-less microbial fuel cell (MFC) because it has biological nanowires(pili) which transfer electrons to outside the cell. In this study, in the anode chamber of the MFC system using G. sulfurreducens, the concentrations of NaCl, sodium phosphate and sodium bicarbonate as electrolytes were mainly optimized for the generation of electricity from acetate. 0.4%(w/v) NaClO and 0.5M $H_2SO_4$ could be utilized for the sterilization of acrylic plates and proton exchange membrane (major construction materials of the MFC reactor), respectively. When NaCl concentration in anode phosphate buffer increased from 5 to 50 mM, power density increased from 6 to $20\;mW/m^2$. However, with increasing sodium phosphate buffer concentration from 5 to 50 mM, power density significantly decreased from 18 to $1\;mW/m^2$. Twenty-four mM sodium bicarbonate did not affect electricity generation as well as pH under 50 mM phosphate buffer condition. Optimized anode chamber of MFC using G. sulfurreducens generated relatively high power density ($20\;mW/m^2$) with the maximum coulombic efficiency (41.3%).

Effects of electron donors and acceptors in generating bioelectrical energy using microbial fuel cells

  • Gurung, Anup;Oh, Sang-Eun
    • 한국환경농학회지
    • /
    • 제31권1호
    • /
    • pp.24-29
    • /
    • 2012
  • BACKGROUND: In recent years, microbial fuel cells (MFCs) have emerged as a promising technology for recovering renewable energy from waste biomass, especially wastewater. In this study, the possibility of bioelectricity generation in two chambered mediator-less microbial fuel cells (MFCs) was successfully demonstrated using fermentable and non-fermentable substrates. METHODS AND RESULTS: Two different electron acceptors have been tested in the cathode chamber for the effects of reducing agent on the power generation in MFCs. The average voltages of $0.26{\pm}0.014$ V and $0.36{\pm}0.02$ V were achieved with acetate using oxygen and potassium ferricyanide as reducing agent, respectively. Similarly, with glucose the average voltages of $0.256{\pm}0.05$ V and $0.340{\pm}0.04$ V were obtained using oxygen and ferricyanide, respectively. Using potassium ferricyanide as the reducing agent, the power output increases by 39 and 43% with acetate and glucose, respectively, as compared to the dissolved oxygen. Slightly higher coulombic efficiency (CE%) was obtained in acetate as compared to MFCs operated with glucose. The maximum power densities of 124 mW/$m^2$ and 204 mW/$m^2$ were obtained using dissolved oxygen and $K_3Fe(CN)_6$, respectively. CONCLUSION(s): This study demonstrates that power generation from the MFCs can be influenced significantly by the different types of catholyte. Relatively higher CE was obtained with $K_3Fe(CN)_6$. Thus, application of $K_3Fe(CN)_6$ as the catholyte can be vital for scaling uppower generation from the MFCs forreal time applications.