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Comparison of Loop-mediated Isothermal Amplification and Korea Standard Food Codex (KFSC) Method for Detection of Salmonella Typhimurium, Listeria monocytogenes Artificially Inoculated in Yuk-hwe and Yuk-sashimi (육회와 육사시미에 접종된 Salmonella Typhimurium와 Listeria monocytogenes 검출을 위한 Loop-mediated isothermal amplification와 식품공전의 배지 시험법, real-time PCR의 검출 성능 비교)

  • Gwak, Seung-Hae;Lee, So-Young;Kim, Jin-Hee;Oh, Se-Wook
    • Journal of Food Hygiene and Safety
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    • v.34 no.3
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    • pp.277-282
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    • 2019
  • The object of this study is to compare the performance of the 3M Molecular Detection Assay 2 (3M MDA 2) and the Korea Standard Food Codex (KSFC) Method (i.e., isolation media and real-time PCR) in detecting Salmonella Typhimurium and Listeria monocytogenes in traditional Korean foods. Yuk-hwe and Yuk-sashimi (types of raw beef dishes) were artificially inoculated with $10^0-10^4CFU/25g$ of L. monocytogenes and S. Typhimurium. Citrobacter freundii and Listeria innocua were used as competitive microflora. After enrichment, the samples were analyzed using 3M MDA 2 and real-time PCR. All samples inoculated at concentrations of $10^0-10^4CFU/25g$ without competitive microflora were positive for S. Typhimurium and L. monocytogenes, as detected by 3M MDA 2 and Korea Standard Food Codex (KFSC) Method. In addition, part of the samples were positive for the presence of C. freundii and L. innocua. The 3M MDA 2 - Salmonella and Korea Standard Food Codex (KFSC) Method showed similar detection performances in Yuk-hwe and Yuk-sashimi. The 3M MDA 2 method for Salmonella and Listeria, which is a LAMP-based technology, can be used for rapid detection of S. Typhimurium and L. monocytogenes in raw beef. LAMP bioluminescence assays provide results on the subsequent day and are simple to use compared with the Korea Standard Food Codex (KFSC) Method, particularly in terms of DNA preparation.

Comparative assessment of urban stormwater low impact strategies equipped with pre-treatment zones (침강지 시설이 조성된 LID 시설의 환경적 영향평가)

  • Yano, K.A.V.;Reyes, N.J.D.G.;Jeon, M.S.;Kim, L.H.
    • Journal of Wetlands Research
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    • v.21 no.2
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    • pp.181-190
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    • 2019
  • Recently, Low impact development techniques, a form of nature-based solutions (NBS), were seen cost-efficient alternatives that can be utilized as alternatives for conventional stormwater management practices. This study evaluated the effectiveness of an infiltration trench (IT) and a small constructed wetland (SCW) in treating urban stormwater runoff. Long-term monitoring data were observed to assess the seasonal performance and cite the advantages and disadvantages of utilizing the facilities. Analyses revealed that the IT has reduced performance during the summer season due to higher runoff volumes that exceeded the facility's storage volume capacity and caused the facility to overflow. On the other hand, the pollutant removal efficiency of the SCW was impacted by the winter season as a result of dormant biological activities. Sediment data also indicated that fine and medium sand particles mostly constituted the trapped sediments in the pretreatment and media zones. Sediments in SCW exhibited a lower COD and TN load due to the phytoremediation and microbiological degradation capabilities of the system. This study presented brief comparison LID facilities equipped with pre-treatment zones. The identified factors that can potentially affect the performance of the systems were also beneficial in establishing metrics on the utilization of similar types of nature-based stormwater management practices.

A Study on Personal Everyday Records in On Kawara's Works (온 카와라(On Kawara) 작품에 나타난 개인의 일상기록 연구)

  • Lee, Hye Rin;Park, Ju Seok
    • The Korean Journal of Archival Studies
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    • no.49
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    • pp.213-246
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    • 2016
  • On Kawara's works are not only artworks but are also his personal daily records. He produced many artworks whose theme centered on his everyday life, a plan that he had precisely made. He selected several specific themes from his daily life: when and where he met someone, what he read, and where he traveled. Kawara constantly recorded his daily life using various types of unconventional medium: maps, postcards, and telegrams. Although he was occupied with recording his everyday life, he was also active in forming public opinions on various issues through social media. Kawara's works, which exhibit a distinctive characteristic of himself, could be classified as individual records because it includes an individual's daily life and selected issues. His artworks express concern for or expose the irrationality of his society, which encourages ordinary people to let their voices be heard and take part in public affairs. Thus, his works function not just as art but also as precious historical records.

A study on the risk factors of the fishermen's in offshore large powered purse seine fishery using the accident compensation insurance proceeds payment data of NFFC (수협의 어선원 재해보상보험 자료를 이용한 대형선망어업 선원의 위험요인 연구)

  • CHOI, Jun-Ik;KIM, Hyung-Seok;LEE, Chun-Woo;OH, Taeg-Yun;SEO, Young-Il;LEE, Yoo-Won;RYU, Kyung-Jin
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.55 no.1
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    • pp.82-93
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    • 2019
  • The International Labor Organization (ILO) selected fishing as the most dangerous group of jobs in the world, and it is well known in Korea as a 4D industry. Offshore large purse seine accounts for the largest portion of the fishing industry in the coastal region with high death rate and the accident rate. The repeated disaster rate survey by the Korea Maritime Institute (KMI) shows offshore large purse seiner is the highest at 22.3 percent and coastal gill nets and offshore stow net are following at 12.4 percent and 11.9 percent in order. Therefore, risk factors occurring in offshore large purse seiners were analyzed based on data from the National Federation of Fisheries Cooperatives (NFFC) for three years from 2015 to 2017 and 4M (Man, Machine, Media, Management) 3E (Engineering, Education, Enforcement) techniques were used to provide a safe fishing environment. The number of accidents on offshore large purse seiners each year was more than 150, and the number of accidents on every fishing boats was as high as 17 percent in 2015. If the accident rate and the risk level were divided by insurance, the accident resulting from contact with machinery was the highest, and the risk of a contact with gear was low but frequently occurring. This was caused by collisions and contact with gear in situations where death and disappearance risk are not high, and accident types in situations where death and loss risk are considered to be contact with machinery, falls or other accidents. Through these analysis techniques, the frequency and risk of each type of accident on a offshore large purse seiners can be demonstrated, and it is expected to raise awareness of a safer fishing environment and contribute to the reduction of accidents.

Performance Analysis of Super-Resolution based Video Coding for HEVC (HEVC 기반 초해상화를 이용한 비디오 부호화 효율 성능 분석)

  • Ki, Sehwan;Kim, Dae-Eun;Jun, Ki Nam;Baek, Seung Ho;Choi, Jeung Won;Kim, Dong Hyun;Kim, Munchurl
    • Journal of Broadcast Engineering
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    • v.24 no.2
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    • pp.306-314
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    • 2019
  • Since the resolutions of videos increase rapidly, there are continuing needs for effective video compression methods despite an increase in the transmission bandwidth. In order to satisfy such a demand, a reconstructive video coding (RVC) method by using a super resolution has been proposed. Since RVC reduces the resolution of the input video, when frames are compressed to the same size, the number of bits per pixel increases, thereby reducing coding artifacts caused by video coding. However, RVC method using super resolution is not effective in all target bitrates. Comparing the size of the loss generated while downsizing the resolution and the size of the loss caused by the video compression, only when the size of loss generated in the video compression is larger, RVC method can perform the improved compression performance compared to direct video coding. In particular, since HEVC has considerably higher compression performance than the previous standard video codec, it can be experimentally confirmed that the compression distortions become larger than the distortions of downsizing the resolution only in the very low-bitrate conditions. In this paper, we applied RVC based HEVC in various video types and measured the target bitrates that RVC method can be effectively applied.

Examining a Case Study of Participation of Science-Related Activities and Personal Characteristics of Two Primary Students on Developed Individual Interest in Science (발달된 개인적 흥미 단계의 두 초등학생들의 과학과 관련된 활동의 참여와 개인적 특성에 대한 사례 탐구)

  • Choi, Yoon-Sung
    • Journal of Science Education
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    • v.45 no.1
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    • pp.69-89
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    • 2021
  • The purpose of this study is to examine personal characteristics of two elementary students who developed individual interest in science. 201 students of two elementary schools in Seoul participated in questionnaire survey engaged in to investigate students' interest in science, engagements and preference in science-related-activities three times a year. This case study was conducted with seven students who showed typical types of interest in science. Students wrote a photo-journal for 12 weeks. They were interviewed every other week by a researcher. We analyzed two students who developed individual interest. It turns out that RF participated in doing experiments at home, and disassembling and assembling things. Participation of activities was a process of solving curiosity. When he was unable to solve problems, he searches the internet or books. Sometimes he would ask for help from his teacher. JW engaged in activities: drawing, doing experiments at home, and going to informal education center. She communicated with others by using online-offline media. Curious questions were solved by herself. The two students have a tendency to solve problems by themselves. Also, positive science-related activities were going on at home and in school. This study not only give insights and understanding of students who developed individual interest in science but also provide implication for educators to examine personal characteristics of students.

Deep Learning Algorithm and Prediction Model Associated with Data Transmission of User-Participating Wearable Devices (사용자 참여형 웨어러블 디바이스 데이터 전송 연계 및 딥러닝 대사증후군 예측 모델)

  • Lee, Hyunsik;Lee, Woongjae;Jeong, Taikyeong
    • Journal of Korea Society of Industrial Information Systems
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    • v.25 no.6
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    • pp.33-45
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    • 2020
  • This paper aims to look at the perspective that the latest cutting-edge technologies are predicting individual diseases in the actual medical environment in a situation where various types of wearable devices are rapidly increasing and used in the healthcare domain. Through the process of collecting, processing, and transmitting data by merging clinical data, genetic data, and life log data through a user-participating wearable device, it presents the process of connecting the learning model and the feedback model in the environment of the Deep Neural Network. In the case of the actual field that has undergone clinical trial procedures of medical IT occurring in such a high-tech medical field, the effect of a specific gene caused by metabolic syndrome on the disease is measured, and clinical information and life log data are merged to process different heterogeneous data. That is, it proves the objective suitability and certainty of the deep neural network of heterogeneous data, and through this, the performance evaluation according to the noise in the actual deep learning environment is performed. In the case of the automatic encoder, we proved that the accuracy and predicted value varying per 1,000 EPOCH are linearly changed several times with the increasing value of the variable.

Analysis of news bigdata on 'Gather Town' using the Bigkinds system

  • Choi, Sui
    • Journal of the Korea Society of Computer and Information
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    • v.27 no.3
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    • pp.53-61
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    • 2022
  • Recent years have drawn a great attention to generation MZ and Metaverse, due to 4th industrial revolution and the development of digital environment that blurs the boundary between reality and virtual reality. Generation MZ approaches the information very differently from the existing generations and uses distinguished communication methods. In terms of learning, they have different motivations, types, skills and build relationships differently. Meanwhile, Metaverse is drawing a great attention as a teaching method that fits traits of gen MZ. Thus, the current research aimed to investigate how to increase the use of Metaverse in Educational Technology. Specifically, this research examined the antecedents of popularity of Gather Town, a platform of Metaverse. Big data of news articles have been collected and analyzed using the Bigkinds system provided by Korea Press Foundation. The analysis revealed, first, a rapid increasing trend of media exposure of Gather Town since July 2021. This suggests a greater utilization of Gather Town in the field of education after the COVID-19 pandemic. Second, Word Association Analysis and Word Cloud Analysis showed high weights on education related words such as 'remote', 'university', and 'freshman', while words like 'Metaverse', 'Metaverse platform', 'Covid19', and 'Avatar' were also emphasized. Third, Network Analysis extracted 'COVID19', 'Avatar', 'University student', 'career', 'YouTube' as keywords. The findings also suggest potential value of Gather Town as an educational tool under COVID19 pandemic. Therefore, this research will contribute to the application and utilization of Gather Town in the field of education.

A Study on High-acidity Rubus coreanus Concentrated Vinegar Production Using Freeze Concentration Method (동결농축법을 이용한 고산도 복분자 농축식초 제조에 관한 연구)

  • Sung, Ji-Youn;Lee, Ikheui
    • Journal of the Korea Convergence Society
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    • v.12 no.11
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    • pp.419-426
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    • 2021
  • Until recently, various studies for the production of high-acidity vinegar have been conducted, but there have been few studies on the concentration process of vinegar. In this study, the possibility of introducing freeze-concentration method was investigated for the production of high-quality, high-acidity vinegar. Acidity, pH, specific gravity, and glucose concentration were measured for frozen concentrated vinegar fractions obtained by freezing and thawing three types of Rubus coreanus vinegar with different acidy (7.53, 5.43, and 3.72). Acidity, specific gravity, and glucose concentration were all highest when 15% of the original vinegar was thawed. pH was the lowest when 15% of the original vinegar was melted. As a result of the measurement of cumulative acidity, when 20% of the original vinegar was melted, it was the highest at 9.32, which was 3.89 higher than that of the original vinegar. In this study, it was confirmed that vinegar can be effectively concentrated using the freeze concentration method and at the same time, the thawing ratio can be controlled to effectively obtain vinegar with the desired acidity. However, it is considered that studies such as organic acid and amino acid analysis are needed to determine whether the freeze-concentration method is introduced into the high-acidity concentrated vinegar manufacturing process.

Analysis of Use Behavior of Urban Park Users Expressing Depression on Social Media Using Text Mining Technique (텍스트 마이닝 기법을 활용한 SNS 상에서 우울감을 언급한 도시공원 이용자의 이용행태 분석)

  • Oh, Jiyeon;Nam, Seongwoo;Lee, Peter Sang-Hoon
    • The Journal of the Korea Contents Association
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    • v.22 no.6
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    • pp.319-328
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    • 2022
  • The purpose of this study was to investigate the relationship between depression due to the COVID-19 pandemic and park use behaviors using on line posts. During the period of the pandemic prevention activities, text data containing both 'park' and 'depression' were collected from blogs and cafes in the search engine of Naver and Daum, then analyzed using Text Mining and Social Network techniques. As a result, the main usage behaviors of park users who mentioned depression were 'look', 'stroll(walk)' and 'eat'. Other types of behaviors were connected centering around 'look', one of the communication behaviors. Also, from CONCOR analysis, as the cluster referred from communication behavior and dynamic behavior was formed as a single behavior type, it was considered park users with depression perceived the park as the space for communication and physical activities. As the spread of COVID-19 caused the restriction of communication activities, the users might consider parks as one of the solutions. In addition, it was considered that passive usage behaviors have prevailed rather than active ones due to the depression. Resulting outcomes would be useful to plan helpful urban park for citizens. It is necessary to further analyze the park use behavior of users in relation to the period of before/after the COVID-19 pandemic and the existence/nonexistence of depression.