• 문화기술의 융합
• /
• 제6권2호
• /
• pp.85-90
• /
• 2020

최근 한류의 영향으로 인하여 한국 육아 예능 프로그램이 중국에서 선풍적 인기를 끌었고 동시에 일부 현지화한 프로그램도 중국에서 큰 인기를 얻었다. 본 연구의 목적은 문화 계발 이론에 근거하여 한국 육아 예능 프로그램 시청이 중국 시청자의 육아 태도와 의식에 미치는 영향에 대해 살펴보고자 하였다. 온라인 설문조사를 통해 총 544명의 중국 시청자를 조사한 결과 한국의 육아 예능 프로그램은 중국 시청자들로부터 많은 사랑을 받았을 뿐만 아니라 그들의 육아태도 및 의식 그리고 행위에 영향을 미친 것으로 나타났다.

• Journal of Microbiology and Biotechnology
• /
• 제23권1호
• /
• pp.47-55
• /
• 2013

Metabolic alterations of Cordyceps bassiana mycelium were investigated under the following culture medium and light conditions: dextrose agar supplemented with 0.5% yeast extract (SDAY) medium with light (SL), SDAY medium without light (SD), nut medium without light (ND), and iron-supplemented SDAY medium without light (FD). The levels of asparagine, aspartic acid, glutamic acid, glutamine, histidine, lysine, ornithine, and proline were significantly higher under SD and SL conditions. The levels of most of the alcohols, saturated fatty acids, unsaturated fatty acids, fatty acid esters, sterols, and terpenes were higher under the ND condition than in the other conditions, but beauvericin was not detectable under the ND condition. The FD condition was favorable for the enhanced production of aminomalonic acid, malic acid, mannonic acid, and erythritol. Thus, the metabolic characteristics of C. bassiana can be manipulated by varying the cultivation conditions, rendering this fungus potentially favorable as a nutraceutical and medicinal resource.

• 한국약용작물학회지
• /
• 제22권3호
• /
• pp.188-195
• /
• 2014

This study was carried to investigate the effect of illite addition on the growth, bioactive components and volatile compounds of leaf on Saururus chinensis Baill cultivation in greenhouse. Illite addition on Saururus chinensis Baill cultivation resulted no significant effects on the aerial part. However, the root part was highest in 5% illite addition group. Crude oil and ash amount increased as illite additives was increased. Ca of inorganics and free sugars contents were highest in 5% and 20% illite addition, respectively. Hyperoside and isoquercetin of available component on leaf were highest in 5% illite addition group, 4.02mg/g and 4.31mg/g, respectively. The volatile compounds in Saururus chinensis Baill leaf cultured with illite addition amounts were isolated by solid-phase microextraction fiber (polydimethysiloxane $65{\mu}m$) and identifed by gas chromatogtaphy mass spectrometry. As the results, the 22 volatile compounds were identified from in Saururus chinensis Baill leaf and major volatile compounds were the ${\alpha}$-cadinol (18.50%), myristicin (16.46%), methyl-9-methyl-tetradecanoate (10.22%), and ${\gamma}$-muurolene (9.75%). Especially, the content of ${\alpha}$-cadinol was highest in 5% illite addition group and ${\gamma}$-muurolene on overall illite addition group was lower than no addition group.

• 미생물학회지
• /
• 제34권1_2호
• /
• pp.37-42
• /
• 1998

토양으로부터 levan을 분해하여 단일종의 fructooligosaccharide를 생산하는 새로운 미생물을 분리 선별하여 본 연구의 공시균주로 선택하였다. Levanase 생산을 위한 최적 배지조성은 0.5% levan, 0.3% yeast extract 0.3% $NaNO_3$, 0.1% $K_2HPO_4$, 0.05% NaCl(pH 8.0)이었으며, 500ml용 shaking flask에 배지 50ml를 넣어 $30^{\circ}C$에서 54시간 배양시켰을 때 목적효소의 생산이 최대에 도달하였다. Levanase에 의해 생성되는 생성물은 단일종의 oligo당임이 확인되었으며 측쇄가 많은 levan으로부터는 소량의 측쇄구조를 가진 oligosaccharide로 추정되는 미지의 물질이 부산물로 생성되었다. 생성 oligo당을 순수하게 정제하여 HPLC 및 ESI-MASS로 중합도를 조사한 결과 DP가 7인 levanheptaose임이 판명되었다.

• 한국관광식음료학회지:관광식음료경영연구
• /
• 제13권1호
• /
• pp.69-80
• /
• 2002

약용버섯의 균사체(Ganoderma lucidum 7094, Fomitella fraxinea 81003, Phellinus igniarius 26005)를 곡류와 같은 고체재료에 배양하고 발효된 곡류의 유리당 조성 변화를 관찰함으로써 기능성식품 개발의 가능성을 검토하였다. 담자균사체의 대량배양 조건 실험을 실시한 결과, 냉침에 의해 최대수화에 도달한 곡물을 배양용기에 담고, 액체배양 후 균질화한 담자균사체를 곡물에 접종함으로써 접종초기 짧은 시간에 균사가 완전히 활착되도록 할 수 있었다. 또한 균사 배양중기에 멸균 증류수를 첨가함으로써 균사의 활력을 유지시킬수 있었다. 담자균사체는 종류에 따라 유리당 총량 및 구성비에 있어서 큰 차이를 나타내었다. 또한 담자균사체가 배양된 곡물의 유리당 함량을 측정한 결과, 유리당 총량 및 대부분의 유리당 함량이 곡물 자체에서 보다 증가하였으며, 담자균과 곡물배지의 종류에 따라 당의 이용성에 큰 차이를 나타내었다.

• The Plant Pathology Journal
• /
• 제35권6호
• /
• pp.674-683
• /
• 2019

Some species of the Trichoderma genus are reported as the major problem in oak wood mushroom production in Korea. In spite of economic loss by the fungi, scientific information on airborne Trichoderma species is not much available. To generate information for disease management development we analyzed airborne Trichoderma. A total of 1,063 fungal isolates were purely obtained from indoor air sampling of cultivation houses used for oak wood mushroom using sawdust media. Among the obtained isolates, 248 isolates were identified as Trichoderma fungi including T. harzianum, T. atroviride, T. citrinoviride, and T. pseudokoningii, by morphological and molecular analysis. T. harzianum was dominant among the four identified species. All the four Trichoderma species grew fast on solid nutrient media tested (potato dextrose agar [PDA], malt extract agar [MEA], Czapek's Dox + yeast extract agar [CYA] and cornmeal dextrose agar). Compact mycelia growth and mass spore production were better on PDA and CYA. In addition, T. harzianum and T. citrinoviride formed greenish and yellowish mycelium and spores on PDA and CYA. Greenish and yellowish pigment was saturated into PDA only by T. pseudokoningii. These four Trichoderma species could produce extracellular enzymes of sawdust substrate degradation such as β-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease. Their mycelia inhibited the growth of oak wood mushroom mycelia of two tested cultivars on dual culture assay. Among of eleven antifungal agents tested, benomyl was the best to inhibit the growth of the four Trichoderma species. Our results demonstrate that the airborne Trichoderma fungi need to be properly managed in the cultivation houses for safe mushroom production.

• 디자인학연구
• /
• 제16권1호
• /
• pp.93-102
• /
• 2003

이 논문은 영농환경 개선을 위한 인삼재배사(일명 해가림 시설물) 디자인 표준화를 위한 연구이다. 연구배경의 핵심은 현 WTO 농산물 개방 체제하에서 한국의 세계적 농특산물인 고려인삼의 생산과 품질 가치를 더욱 확고히 하고 인삼 종주국으로서 위상에 걸맞는 현대식 인삼재배 시설을 최초로 도입하기 위한 기초연구를 제안함에 있다. 디자인 개념으로서는, 현재 목재 볏짚 비닐 등 일회성 또는 비표준화된 재료를 사용하므로서 매년 반복 재설치 작업 및 환경오염이 되고 있는 우리나라 인삼경작사의 노동 및 자원 낭비적 요소를 개선하고자 하였다. 구체적 개선 요소로서는 첫째, 양산체제에 의한 형태 및 구조의 표준화. 둘째, 반영구적 재료 표준화를 위한 수지 등 신소재 도입, 위 두 가지 내용을 한국적 이미지를 바탕으로 형상화 하고자 하였다. 디자인 결과로서, 인삼재배사의 기준 단위모델을 디자인하여 재배면적의 크기 형상에 따라 가변적으로 연결 조립하여 사용할 수 있도록 배려하였다.

• 한국양식학회지
• /
• 제16권1호
• /
• pp.44-50
• /
• 2003

가시파래의 자연 서식지에서 자연채묘 방법과 실내에서 포자방출에 의한 인공종묘생산 기술을 개발하고, 양성시험에 의한 완전한 양식방법을 개발하여 대량생산 가능성을 조사하였다. 가시파래 자연채묘는 자연 서식지의 사니질로 조성된 해역이 적합하였으며, 하계의 착생밀도는 채묘틀이 지면에서 20cm 높이에 시설하는 것이 높았고, 추계에는 40cm 높이에 시설하는 것이 효과적이었다. 가시파래 인공종묘 생산은 성숙한 모조를 12~24시간 음건하여 포자를 방출하고, 포자액이 들어있는 수조에 채묘망을 넣고 24시간 암흑처리하면 포자 착생률이 높게 나타났다. 가시파래 양성은 수심이 낮고 저질이 니질 또는 사니질로 조성된 해역은 지주식으로 시설하며, 수심이 깊고 유속이 빠른 해역은 부류식으로 시설하는 것이 좋았다. 양성장소는 영양염이 풍부하고 파랑에 의한 엽체의 탈락과 시설물 파손이 적은 해역이 양호하였으며, 광합성률을 높이기 위하여 표층노출식으로 시설하는 것이 생산량이 많았다.

• 한국패류학회지
• /
• 제31권1호
• /
• pp.61-67
• /
• 2015

The occurrence of 'algal bloom', caused by the mass proliferation of phytoplankton, causes serious problems in streams and lakes in Korea. Therefore, in this study, the phytoplankton filter-feeding trait of Unio douglasiae, a type of freshwater clam, was used to reduce the algal bloom in outdoor water tanks during the summer. This involved the construction of a U. douglasiae cultivation apparatus, wherein 1,000 clams were divided into 8 rectangular baskets arranged in the shape of an empty square. The control tank was manufactured in exactly the same shape within the water tank, but without the addition of clams. The algal bloom-reducing effect of U. douglasiae was confirmed by the measurement of (and comparing between) the water quality at the center and periphery of the test and control cultivation apparatus. Water quality measurements included the measurement of water temperature, pH, turbidity, dissolved oxygen (DO) content, and chlorophyll-${\alpha}$ concentrations; the water quality was measured twice a month between June and November 2014.The results of these analyses did not show a significant difference in water quality (temperature, pH, turbidity, DO) between the center and periphery of the test and control tanks. However, the chlorophyll-${\alpha}$ concentration was observed to be much lower at the center of the test tank compared to that at the center and periphery of the control tank, as well as at the periphery of the test tank. This was believed to be a result of the U. douglasiae surrounding the center of the test tank, which prevented the influx of plankton from the periphery. Accordingly, the results of these analyses suggest the possibility that U. douglasiae cultivation could reduce the proliferation of algal blooms in lakes and streams during the summer. In particular, these results indicate possible improvements in U. douglasiae activity (reduction in algal blooms) by their effective arrangement in the water bodies.

• 한국약용작물학회지
• /
• 제24권2호
• /
• pp.93-100
• /
• 2016

Background: A set of logical criteria that can accurately identify and verify the cultivation region of raw materials is a critical tool for the scientific management of traditional herbal medicine. Methods and Results: Volatile compounds were obtained from 19 and 32 samples of Angelica gigas Nakai cultivated in Korea and China, respectively, by using steam distillation extraction. The metabolites were identified using GC/MS by querying against the NIST reference library. Data binning was performed to normalize the number of variables used in statistical analysis. Multivariate statistical analyses, such as Principal Component Analysis (PCA), Partial Least Squares-Discriminant Analysis (PLS-DA), and Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA) were performed using the SIMCA-P software. Significant variables with a Variable Importance in the Projection (VIP) score higher than 1.0 as obtained through OPLS-DA and those that resulted in p-values less than 0.05 through one-way ANOVA were selected to verify the marker compounds. Among the 19 variables extracted, styrene, ${\alpha}$-pinene, and ${\beta}$-terpinene were selected as markers to indicate the origin of A. gigas. Conclusions: The statistical model developed was suitable for determination of the geographical origin of A. gigas. The cultivation regions of six Korean and eight Chinese A. gigas. samples were predicted using the established OPLS-DA model and it was confirmed that 13 of the 14 samples were accurately classified.