• 대한기계학회논문집
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• 제18권9호
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• pp.2349-2357
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• 1994

This paper presents a facial expression transformation algorithm and drawing rule generation algolithm for a portrait drawing robot which was developed for the '93 Taejeon EXPO. The developed algorithm was mainly focused on the robust automatic generation of robot programs with the consideration that the drawing robot should work without any limitation of the age, sex or race for the persons. In order to give more demonstratin effects, the facial expression change of the pictured person was performed.

• 사회복지연구
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• 제49권1호
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• pp.163-190
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• 2018

서울시에서 추진하고 있는 복지생태계조성사업, 마을공동체지향복지관 변화지원 사업과 최근 발의된 <마을공동체기본법> 등 주민주도성은 지역복지활동의 방향성으로 중요하게 부상하고 있는 개념이다. 그러나 주민주도성 개념이 무엇인지에 대한 합의 없이 사용되고 있어, 현장에서 활용되는데 한계가 지적되고 있다. 본 연구는 개념도 연구법(Concept mapping)을 적용하여 서울시내 복지관의 실무자 10명을 대상으로 주민주도성에 대한 59개의 진술문을 생성하여 개념지도를 그리고 다차원척도분석과 위계적 군집분석을 실시하여 총 6개의 범주를 도출하였다. 6개의 범주는 "참여유도", "실천", "절차", "인식과 관심 확장", "의견표출", "태도와 정서"로 명명할 수 있었으며, 주민주도성 개념에서 "실천"이 핵심범주로 드러났다. 본 연구는 주민주도성의 개념에 대해 실제 사회복지실천현장의 실무자들은 어떻게 정의하고 있는지, 또 주민주도성을 실현하기 위해 사회복지사의 역할은 어떠해야 하는지에 대한 고민을 논의하고 향후 합의된 정의를 만들어가는 첫 시도로서 그 의의가 있다.

• 인지과학
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• 제14권1호
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• pp.10-10
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• 2003

본 논문은 Gabor 웨이브렛 변환을 이용하여 무표정을 포함한 표정영상에서 얼굴의 주요 요소들의 경계선을 추출한 후, FCM 군집화 알고리즘을 적용하여 무표정 영상에서 저차원의 대표적인 특징점을 추출한다. 무표정 영상의 특징점들은 표정영상의 특징점들을 추출하기 위한 템플릿으로 사용되어지며, 표정영상의 특징점 추출은 무표정 영상의 특징점과 동적 연결모형을 이용하여 개략적인 정합과 정밀한 정합 과정의 두단계로 이루어진다. 본 논문에서는 Gabor 웨이브렛과 FCM 군집화 알고리즘을 기반으로 동적 연결모형을 이용하여 표정영상에서 특징점들을 자동으로 추출할 수 있음을 제시한다. 본 연구결과는 자동 특징추출을 이용한 차원모형기반 얼굴 표정인식[1]에서 얼굴표정의 특징점을 자동으로 추출하는 데 적용되었다.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1241-1245
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• 2015

The aim of the present work was to examine the putative promoter region of the operon ansPAB and to determine the general elements required for the regulation of transcriptional activity. The transcriptional start site of the ansPAB promoter was determined by using highresolution S1-nuclease mapping. Sequence analysis of this region showed -10 and -35 elements, which were consistent with consensus sequences for R. etli promoters that are recognized by the major form of RNA polymerase containing the σ⁷⁰ transcription factor. Mutation studies affecting several regions located upstream of the transcriptional start site confirmed the importance of these elements on transcriptional expression.

• 한국도서관정보학회지
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• 제51권1호
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• pp.1-20
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• 2020

본고는 2019년 RDA Toolkit Beta가 개발됨에 따라 BIBFRAME에서 RDA Toolkit Beta 대표표현형 요소의 적용 방안을 모색하기 위해 연구방법으로 문헌연구와 RDA Toolkit Beta와 BIBFRAME 속성의 매핑방법을 사용하였다. BIBFRAME에서 RDA Toolkit Beta의 대표표현형 요소를 적용하기 위한 방안으로 다음의 사항을 제안하였다. 첫째, RDA Toolkit Beta에서는 표현형의 속성과 저작으로 전가된 대표표현형 요소를 별도로 구분하고 있으므로 BIBFRAME에서도 저작으로 전가된 대표표현형 요소를 구분하여 정의할 필요가 있다. 이를 위해 (1) 대표표현형을 위한 요소를 완전히 새로 개발하는 것 (2) RepresentativeExpression이라는 한정어를 정의하고 이를 기존 속성에 결합하여 대표표현형 속성으로 사용하는 것 (3) 클래스를 기존 속성에 결합해 대표표현형 속성으로 구분해 사용하는 것의 3가지 방안을 제시하였다. 둘째, BIBFRAME에서 저작과 저작에 대표표현형 속성을 제공한 대표표현형을 상호 연계시킬 수 있는 관계 요소로 를 추가할 것을 제시하였다. 본고는 LRM을 반영한 RDA를 BIBFRAME에서 적용하는 방안을 모색하였다는 점에서 추후 BIBFRAME의 개정에도 기여할 수 있을 것이다.

• Genomics & Informatics
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• 제13권2호
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• pp.40-44
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• 2015

DNA microarray and next-generation sequencing provide data that can be used for the genetic analysis of multiple quantitative traits such as gene expression levels, transcription factor binding profiles, and epigenetic signatures. In particular, chromatin opening is tightly coupled with gene transcription. To understand how these two processes are genetically regulated and associated with each other, we examined the changes of chromatin accessibility and gene expression in response to genetic variation by means of quantitative trait loci mapping. Regulatory patterns commonly observed in yeast and human across different technical platforms and experimental designs suggest a higher genetic complexity of transcription regulation in contrast to a more robust genetic architecture of chromatin regulation.

• 한국발생생물학회지:발생과생식
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• 제21권4호
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• pp.449-456
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• 2017

The germline stem cells of the Drosophila ovary continuously produce eggs throughout the life-span. Intricate regulation of stemness and differentiation is critical to this continuous production. The translational regulator Nos is an intrinsic factor that is required for maintenance of stemness in germline stem cells. Nos expression is reduced in differentiating cells at the post-transcriptional level by diverse translational regulators. However, molecular mechanisms underlying Nos repression are not completely understood. Through three distinct protein-protein interaction experiments, we identified specific molecular interactions between translational regulators involved in Nos repression. Our findings suggest a model in which protein complexes assemble on the 3' untranslated region of Nos mRNA in order to regulate Nos expression at the post-transcriptional level.

• Molecules and Cells
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• 제42권5호
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• pp.418-425
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• 2019

Multicistronic elements, such as the internal ribosome entry site (IRES) and 2A-like cleavage sequence, serve crucial roles in the eukaryotic ectopic expression of exogenous genes. For utilization of multicistronic elements, the cleavage efficiency and order of elements in multicistronic vectors have been investigated; however, the dynamics of multicistronic element-mediated expression remains unclear. Here, we investigated the dynamics of encephalomyocarditis virus (EMCV) IRES- and porcine teschovirus-1 2A (p2A)-mediated expression. By utilizing real-time fluorescent imaging at a minute-level resolution, we monitored the expression of fluorescent reporters bridged by either EMCV IRES or p2A in two independent cultured cell lines, HEK293 and Neuro2a. We observed significant correlations for the two fluorescent reporters in both multicistronic elements, with a higher correlation coefficient for p2A in HEK293 but similar coefficients for IRES-mediated expression and p2A-mediated expression in Neuro2a. We further analyzed the causal relationship of multicistronic elements by convergent cross mapping (CCM). CCM revealed that in all four conditions examined, the expression of the preceding gene causally affected the dynamics of the subsequent gene. As with the cross correlation, the predictive skill of p2A was higher than that of IRES in HEK293, while the predictive skills of the two multicistronic elements were indistinguishable in Neuro2a. To summarize, we report a significant temporal correlation in both EMCV IRES- and p2A-mediated expression based on the simple bicistronic vector and real-time fluorescent monitoring. The current system also provides a valuable platform to examine the dynamic aspects of expression mediated by diverse multicistronic elements under various physiological conditions.

• Asian-Australasian Journal of Animal Sciences
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• 제19권2호
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• pp.165-170
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• 2006

Using the somatic cell hybrid panel (SCHP) and radiation hybrid (IMpRH) panel, porcine SOCS2 gene was mapped at SSC5 (1/2) q21-q24 and closely linked with SW1383 marker (47 cR in distance), while SOCS3 gene was assigned to SSC12p11-(2/3p13) and closely linked with SW2490 (43 cR). The reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect the expression of these two genes in the different tissues and the results showed that both SOCS2 and SOCS3 genes were widely expressed in tissues investigated (heart, liver, spleen, lung, kidney skeletal muscle, fat and brain), although some tissues showed lower gene expression. Moreover, SOCS2 and SOCS3 genes had different expression levels at different stages, in different tissues and in different breeds. A G/A substitution, which can be recognized by restriction enzyme of Cfr421, was observed in 5' untranslated region (5'-UTR) of SOCS2 gene. The allele frequencies was investigated by PCR-restriction fragment length polymorphism (PCR-RFLP) method and it showed that the allele frequency among Dahuabai, Erhualian, Yushan, Qingping, Large white and Landrace tested were different. Association analysis in a cross experimental populations revealed no significant association between the SOCS2 gene polymorphism and the economic traits investigated. The full-length coding regions (CDs) of porcine SOCS3 gene was obtained by RT-PCR.

• Molecules and Cells
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• 제43권7호
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• pp.591-599
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• 2020

Complex cell-to-cell communication underlies the basic processes essential for homeostasis in the given tissue architecture. Obtaining quantitative gene-expression of cells in their native context has significantly advanced through single-cell RNA sequencing technologies along with mechanical and enzymatic tissue manipulation. This approach, however, is largely reliant on the physical dissociation of individual cells from the tissue, thus, resulting in a library with unaccounted positional information. To overcome this, positional information can be obtained by integrating imaging and positional barcoding. Collectively, spatial transcriptomics strategies provide tissue architecture-dependent as well as position-dependent cellular functions. This review discusses the current technologies for spatial transcriptomics ranging from the methods combining mechanical dissociation and single-cell RNA sequencing to computational spatial re-mapping.