Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of the σ70-Dependent Promoter Controlling Expression of the ansPAB Operon of the Nitrogen-Fixing Bacterium Rhizobium etli

  • Angelica, Moreno-Enriquez (Escuela de Medicina, Universidad Marista de Merida) ;
  • Zahaed, Evangelista-Martinez (Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco AC, Unidad Sureste, Parque Cientifico de Yucatan) ;
  • Luis, Servin-Gonzalez (Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, D.F.) ;
  • Maria Elena, Flores-Carrasco (Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, D.F.)
  • Received : 2015.03.04
  • Accepted : 2015.04.02
  • Published : 2015.09.28
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Abstract

The aim of the present work was to examine the putative promoter region of the operon ansPAB and to determine the general elements required for the regulation of transcriptional activity. The transcriptional start site of the ansPAB promoter was determined by using highresolution S1-nuclease mapping. Sequence analysis of this region showed -10 and -35 elements, which were consistent with consensus sequences for R. etli promoters that are recognized by the major form of RNA polymerase containing the σ70 transcription factor. Mutation studies affecting several regions located upstream of the transcriptional start site confirmed the importance of these elements on transcriptional expression.

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References

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