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Analysis of the Adsorbed Plasma Proteins in the Moving Actuator type Total Artificial Heart

  • Gyu Ha Ryu;Jon
    • Journal of Biomedical Engineering Research
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    • v.14 no.4
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    • pp.307-314
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    • 1993
  • Plasma protein adsorption is the first event in the blood-material interaction and influenc- es subsequent platelet adhesion towards thlㅈombus formation. Thiㅈomboembolic events are strongly influenced by surface characteristics of materials and fluid dynamics inside the blood pump. In vitro flow visualizaion and an amimal experiment with the moving actuator type TAH were Performed in order to investigate fluid dynamic effects on the protein adsorption. The diffel'encl level, j of shear rate inside the ventricle Lvere determined by consid- ering the direction of the major opening of four healt valves in the implanted TAH and the visualized flow patterns as well. Each ventricle of the explanted TAH was sectionalized into 12 segments according to the shear rate level. The adsorbed protein on each segment was quantified using the ELISA method after soaking in 2% (wye)SDS/PBS for two days. Adsorbed protein layer thicknesses Itvere measured by the Immunogotd method under TEM. The SEM observation show that right ventricle (RV) , immobilized with albumin, displayed different degrees of platelet adhesion on each segment, whereas the left ventricle (LV), grafted by PEO-sulronate, indicated nearly , iame platelet adhesion behavior, regardless of shear rates. The surface concentrations of adsorbed proteins in the low shear rate region are hlghel'than those in the high region, which was confirmed statistically. A modified adsorption model of plasma protein onto polyurethane surface was suggested by considering the effect of the fluid dynamic characteristics.

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New Cryptand Complexes of Lanthanides(Ⅲ) and Dioxouranium(Ⅵ) Nitrates

  • Oh-Jin Jung;Chil-Nam Choi;Hak-Jin Jung
    • Bulletin of the Korean Chemical Society
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    • v.12 no.2
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    • pp.130-137
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    • 1991
  • The following new cryptand 221 complexes of lanthanides(Ⅲ) and dioxouranium(Ⅵ) nitrate have been synthesized: $(Ln(C_{16}H_{32}N_2O_5)(H_2O)_2(NO_3)_3\ and \((UO_2)_2(C_{16}H_{32}N_2O_5)(H_2O)_4(NO_3)_4$. These complexes have been identified by elemental analysis, moisture titration, conductivity measurements and various spectroscopic techniques. The proton and carbon-13 NMR as well as calorimetric measurements were used to study the interaction of cryptand 221 with La(Ⅲ), Pr(Ⅲ ), Ho(Ⅲ) and $UO_2(Ⅱ)$ ions in nonaqueous solvents. The bands of metal-oxygen atoms, metal-nitrogen atoms and O-U-O in the IR spectra shift upon complexation to lower frequencies, and the vibrational spectra ({\delta}NMN$) of metal-amide complexes in the crystalline state exhibit lattice vibrations below 300 $cm^{-1}$. The NMR spectra of the lanthanides(Ⅲ) and dioxouranium(Ⅵ) nitrate complexes in nonaqueous solvents are quite different, indicating that the ligand exists in different conformation, and also the $^1H$ and $^{13}C-NMR$ studies indicated that the nitrogen atom of the ring has greater affinity to metal ions than does the oxygen atom, and the planalities of the ring are lost by complexation with metal ions. Calorimetric measurements show that cryptand 221 forms more stable complexes with $La^{3+}$ and $Pr^{3+}$ ions than with $UO^{22+}$ ion, and $La^{3+}/Pr^{3+}$ and $UO^{22+}/Pr^{3+}$ selectivity depends on the solvents. These changes on the stabilities are dependent on the basicity of the ligand and the size of the metal ions. The absorption band (230-260 nm) of the complex which arises from the direct interaction of macrocyclic donor atoms with the metal ion is due to n-{\delta}*$ transition and also that (640-675 nm) of $UO^{22+}$-cryptand 221 complex, which arises from interaction between two-dioxouranium(Ⅵ) ions in being out of cavity of the ligand ring is due to d-d* transition.

The Impacts of Physical Health on Health Behaviors of The Korean Rural Elderly (농촌노인의 건강증진행위 관련 요인 - 신체적 건강의 매개효과를 중심으로 -)

  • Lee, Jeong-Hwa;Kim, Kyoung-Shin;Kim, Kyoung-Myoung
    • Journal of the Korean Home Economics Association
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    • v.45 no.3
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    • pp.57-69
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    • 2007
  • The purpose of this study is to examine the impacts of the physical health on health behaviors of the rural elderly. In this study, physical health is measured self-rated health and farmer symptoms. The specific questions addressed in this study are: What are the general tendencies of health behavior, self-rated health and farmer symptoms? What are the direct impacts and indirect impacts of respondents' characteristics, self-rated health and farmer symptoms on health behaviors of the rural elderly? For this purpose, survey data was gathered from 881 rural elderly who live in a village. The statistical methods used for data analysis were descriptive statistics, correlations, and path analysis with spsswin 12.0 program. The major findings of this study are as follows: The level of health behaviors and self-rated health is low and the level of farmer symptoms is high. In general, it can be said that respondents of this study have the low level of physical health and health behaviors. A path analysis shows the relation of variables, which influence on health behaviors. Economic status, education status and farmer symptoms play direct and positive effects on health behaviors. Especially, the effect of farmer symptoms is more important than the others. Sex and age are indirectly significant on health behavior. The results obtained from the study confirm that objective physical health, like as farmer symptoms, plays important roles in health behavior of the rural elderly. It means that the Korean rural elderly need many facilities and services to promote their physical health.

Effect of Macro-nutrients for the Salidroside Production from Callus Cultures of Rhodiola sachalinesis A. Bor (참돌꽃의 캘러스로부터 salidroside 생산에 미치는 배지성분의 영향)

  • Lee, Jae-Seung;Choi, Hye-Jin;Hwang, Baik;Park, Hyoung-Jae;Ahn, Jun-Cheul;Hwang, Sung-Jin
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.4
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    • pp.246-250
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    • 2007
  • Rhodiola sachalinensis A. Bor is a Chinese herb containing the natural compound salidroside, which has been known to possess medical properties such as enhancing bodies' ability to survive in adverse environments and extending human life. To improve a metabolite production from Rhodiola sachalinensis callus cultures, the concentrations of macro nutrients were investigated. To investigate the salidroside production in Rhodiola sachalinensis callus cultures, we analyzed salidroside content in each callus which was cultured in 30 $m{\ell}$ of $2\;{\times}\;B_5$ liquid medium for 4 weeks. The optimal concentrations of macro nutrients for salidroside production ($67.96\;{\pm}\;3.41\;mg/{\ell}$) were found to be 99 mM $KNO_3$, 1 mM $(NH_4)_2SO_4$, 2 mM $NaH_2PO_4{\cdot}2H_2O$, 2 mM $CaCl_2{\cdot}2H_2O$ and 1 mM $MgSO_4{\cdot}7H_2O$. From these results, we determined the more upgraded culture condition for industrial production of salidroside.

A verification on the physical effectiveness of therapeutic horseback riding exercise: Focused on the EMG analysis

  • Kim, You-Sin;Yang, Jae-Young;Lee, Namju
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.2
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    • pp.317-324
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    • 2018
  • Various studies related to therapeutic horseback riding have been reported to be positive for the therapeutic effect of patients with cerebral palsy; however, most of the previous studies focused on to muscle development with training period related to the physical effects of therapeutic horseback riding. To identify the causes and phenomena of muscular activation of the body through actual therapeutic horseback riding exercise and to promote the excellence of physical effects of therapeutic horseback riding. This study was a nonrandomized prospective positive-controlled trial design. Twelve teenaged males with cerebral palsy were selected who had experienced riding exercise for 8-12 months. This study measured 8 muscle activities of the pectoralis major muscle (PM), biceps brachii (BB), rectus abdominis muscle (RA), latissimus dorsi muscle (LD), spinal erector muscle (SE), rectus femoris muscle (RF), anterior tibial muscle (AT), and external gastrocnemius muscle (EG) by using electromyography (EMG). Muscle activity was significantly higher in horse riding position than sitting on the common chair in all muscles (PM, BB, RA, LD, SE, RF, AT, and EG). The activity of the body muscles according to the difference of horse walking method (walk: WA; sitting trot: ST; and riding trot: RT) of therapeutic horse riding showed the highest muscle activity in the PM muscle at ST, and the highest activity at BB, RA, LD, SE, and AT muscles at ST and RT, and showed the highest muscle activity in RF and EG muscle at RT. The results of this study suggest that intervention for the treatment of cerebral palsy patients can use therapeutic riding exercise as a rehabilitation method.

Health and Safety Management Issues of Migrant Workers in Manufacturing Industries and Workplace Enhancement (제조업사업장 외국인근로자 안전관리 실태와 개선방안연구)

  • Kawshalya, Mailan Arachchige Don Rajitha;Weerasinghe, S.D.N.A.M. Amila Madhushanka;Kim, Sang-Hoon;Lim, Hyung-Duk
    • Journal of the Korean Society of Industry Convergence
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    • v.25 no.2_2
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    • pp.257-262
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    • 2022
  • With the shortage of labor force in small and medium-size industries, the Korean Employment Permit System (EPS) continues to bring migrant workers. However, the question remains of the health and safety management of their migrant workers. Considering that a significant part of safety and health problems are caused by migrant workers due to a lack of information on identifying risk and inexperience in the field. Telephone interviews were conducted to investigate the migrant workers' perspectives on workplace safety practices. The survey focused on workers' accident histories, understanding of safety practices, and responding to industrial accidents. A total of 66 accidents occurred among 30 participants. 10 accidents were not reported to the employer (company), which the foreign workers judged to be minor injuries that should not be reported as accidents. 80.0% of the participants expressed dissatisfaction with the PPE they were using. Among various reasons lack of understanding due to language barriers, lack of awareness of the importance of safety, communication difficulties were major reasons for the caused accidents and potential health issues. It is necessary to improve the safety and health support system by providing practical support through specialized educational institutes with experts in the field. A legal framework and a proper safety management system need to be maintained. This study suggests changing the current policy to improve the health and safety of migrant workers by, establishing specialized educational institutes with locally grown foreign experts in the field.

Conditioning-induced cardioprotection: Aging as a confounding factor

  • Randhawa, Puneet Kaur;Bali, Anjana;Virdi, Jasleen Kaur;Jaggi, Amteshwar Singh
    • The Korean Journal of Physiology and Pharmacology
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    • v.22 no.5
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    • pp.467-479
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    • 2018
  • The aging process induces a plethora of changes in the body including alterations in hormonal regulation and metabolism in various organs including the heart. Aging is associated with marked increase in the vulnerability of the heart to ischemia-reperfusion injury. Furthermore, it significantly hampers the development of adaptive response to various forms of conditioning stimuli (pre/post/remote conditioning). Aging significantly impairs the activation of signaling pathways that mediate preconditioning-induced cardioprotection. It possibly impairs the uptake and release of adenosine, decreases the number of adenosine transporter sites and down-regulates the transcription of adenosine receptors in the myocardium to attenuate adenosine-mediated cardioprotection. Furthermore, aging decreases the expression of peroxisome proliferator-activated receptor gamma co-activator 1-alpha ($PGC-1{\alpha}$) and subsequent transcription of catalase enzyme which subsequently increases the oxidative stress and decreases the responsiveness to preconditioning stimuli in the senescent diabetic hearts. In addition, in the aged rat hearts, the conditioning stimulus fails to phosphorylate Akt kinase that is required for mediating cardioprotective signaling in the heart. Moreover, aging increases the concentration of $Na^+$ and $K^+$, connexin expression and caveolin abundance in the myocardium and increases the susceptibility to ischemia-reperfusion injury. In addition, aging also reduces the responsiveness to conditioning stimuli possibly due to reduced kinase signaling and reduced STAT-3 phosphorylation. However, aging is associated with an increase in MKP-1 phosphorylation, which dephosphorylates (deactivates) mitogen activated protein kinase that is involved in cardioprotective signaling. The present review describes aging as one of the major confounding factors in attenuating remote ischemic preconditioning-induced cardioprotection along with the possible mechanisms.

An Analysis of Reliability by Factors for Development of Job Stress Measurement Tools of Radiological Technologists (방사선사의 직무 스트레스 측정도구 개발을 위한 요인별 신뢰도 분석)

  • Jung, Hong-Ryang;Son, Bu-Soon;Lim, Cheong-Hwan
    • Journal of Environmental Health Sciences
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    • v.32 no.2 s.89
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    • pp.103-110
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    • 2006
  • The present study is designed to analyze reliability and validity of the instruments used to measure job stress of radiological technologists mainly by means of the questionnaires from 'Korean edition of NIOSH check list'. The subjects of this study are 890 radiological technologists active at 44 general hospitals in 16 provinces and cities across the country. The item-total reliability revealed that 10 factors in 6 are the major causes of stress in job life of radiological technologists. The measuring instrument proved to be valid with reliability coefficient of internal consistency by factors being more than 0.7. The factors found to be applicable herewith include limit and authority of job (0.73), cohesion within departments (0.86), resources control (0.81), mental requirements (0.85), job load (0.82), and job stability (0.72). When job autonomy item of causes are modified for proper use to radiological technologists, the measuring instruments are expected to show high reliability. It seems, however, necessary to develop a measuring instrument to analyze and use the validity of related measuring instruments since the reliability here was rated low, with statistical coefficients lower than 0.7 in such factors as job management (0.57), conflicts within departments (-1.13), sense of responsibility for patients (0.66) and usage of education (0.26). As this study was intended to examine reliability and validity of the concepts related to measurement of job stress on the part of radiological technologists, it nay not be proper to apply the results of this study to general use, but is advised to utilize them as basis for developing instruments to verify reliability and validity by comparing with and analyzing other measuring instruments.

Comparison of the Chemical Compositions and Biogenic Amine Contents of Salt-fermented Fish Sauces Produced in Korea to Evaluate the Quality Characteristics (시판멸치액젓 및 까나리액젓의 품질특성 평가를 위한 이화학적 성분 및 Biogenic amine 함량 비교)

  • Kim, Bo-Kyoung;Kim, Yong-Hoon;Lee, Hong-Hee;Cho, Young-Je;Kim, Dae-Sik;Oh, Sang-Min;Shim, Kil-Bo
    • Journal of Fisheries and Marine Sciences Education
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    • v.23 no.4
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    • pp.607-614
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    • 2011
  • This study was conducted to understand the quality characteristics of 20 kinds of commercial salt-fermented anchovy & sand lance sauces by measuring their the chemical compositions and 9 biogenic amines (tryptamine, 2-phenylethylamine, putrescin, cadaverine, histamine, tyramine, serotonin, noradrenaline, spermine) contents. The commercial salt-fermented anchovy sauces contained $65.84{\pm}0.11{\sim}70.60{\pm}0.21%$ of moisture, $20.50{\pm}0.41{\sim}25.60{\pm}0.42%$ of salinity, $0.98{\pm}0.01{\sim}2.05{\pm}0.05%$ total nitrogen, and $1,011.77{\pm}0.00{\sim}1,724.56{\pm}9.72mg/100mL$ of amino nitrogen. Histamine was the major amine detected in salt-fermented fish sauces and it was varied from 421.27 to 1,507.18 mg/kg in salt-fermented anchovy sauces, whereas commercial salt-fermented sand lance sauces contained $67.87{\pm}0.28{\sim}69.63{\pm}0.17%$ moisture, $22.46{\pm}0.28{\sim}26.11{\pm}0.00%$ salinity, $0.92{\pm}0.01{\sim}1.71{\pm}0.05%$ total nitrogen, $878.20{\pm}0.00{\sim}1430.09{\pm}9.77mg/100mL$ amino nitrogen and 419.10~1,025.50 mg/kg histamine, respectively. These findings suggest that the products of salt-fermented fish sauces have pretty much the same in ingredient composition and meet domestic criteria but most those had high biogenic amine contents. Therefore, ingredient composition and biogenic amine content of commercial salt-fermented fish sauce products were practicable evaluation of the quality characteristics.

The Effects of Variables on gender equality consciousness by Middle School Students (중학생의 양성평등의식에 영향을 미치는 변인)

  • Lee, Jeong-Dae;Lee, Seon-Jeong;Shin, Hyo-Shick
    • Journal of Korean Home Economics Education Association
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    • v.22 no.2
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    • pp.15-30
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    • 2010
  • The purposes of this study were intended to examine by variables influencing gender equality consciousness in middle school students and provide basic materials useful for their education of gender equality consciousness by finding out variables influencing equality consciousness. The subjects were the 1,201 students who studied at middle schools in Jeonllabuk-do. Using structured questionnaire surveyed. Data were analyzed with Frequency, Percentage, Mean, Std, Cronbach's ${\alpha}$, t-test, and Multiple Regression using SPSS/PC WIN 14.0 program. The major findings were as follows: 1. The mean value of gender equality consciousness in middle school students was 3.89 in the whole, 3.88 in home life, 3.92 in school life, 4.04 in the workplace, and 3.70 in social and cultural life, suggesting high value in all areas. While the workplace and the school life showed higher gender equality consciousness than the whole, the home life and social and cultural life showed lower consciousness. 2. The whole gender equality consciousness were influenced by sex, mother's gender equality consciousness, homeroom teacher's gender equality consciousness, an enough schooling, desirable school types, perception of abolition of family-head system, and social gender equality consciousness. And the Middle school students' whole gender equality consciousness were explained about 32% by these variables.

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