Effect of Macro-nutrients for the Salidroside Production from Callus Cultures of Rhodiola sachalinesis A. Bor

참돌꽃의 캘러스로부터 salidroside 생산에 미치는 배지성분의 영향

  • Lee, Jae-Seung (Major of Biological Education, Graduate School of Education, Sunchon National University) ;
  • Choi, Hye-Jin (Department of Biology, Chonnam National University) ;
  • Hwang, Baik (Department of Biology, Chonnam National University) ;
  • Park, Hyoung-Jae (Plant Biotechnology, School of Bioscience & Biotechnology, Kangwon Natl. Univ.) ;
  • Ahn, Jun-Cheul (Plant Biotechnology, School of Bioscience & Biotechnology, Kangwon Natl. Univ.) ;
  • Hwang, Sung-Jin (Department of Biology, Chonnam National University)
  • 이재승 (순천대학교 교육대학원 생물교육) ;
  • 최혜진 (전남대학교 생물학과) ;
  • 황백 (전남대학교 생물학과) ;
  • 박형재 (강원대학교 BT특성화학부대학) ;
  • 안준철 (강원대학교 BT특성화학부대학) ;
  • 황성진 (전남대학교 생물학과)
  • Published : 2007.08.30

Abstract

Rhodiola sachalinensis A. Bor is a Chinese herb containing the natural compound salidroside, which has been known to possess medical properties such as enhancing bodies' ability to survive in adverse environments and extending human life. To improve a metabolite production from Rhodiola sachalinensis callus cultures, the concentrations of macro nutrients were investigated. To investigate the salidroside production in Rhodiola sachalinensis callus cultures, we analyzed salidroside content in each callus which was cultured in 30 $m{\ell}$ of $2\;{\times}\;B_5$ liquid medium for 4 weeks. The optimal concentrations of macro nutrients for salidroside production ($67.96\;{\pm}\;3.41\;mg/{\ell}$) were found to be 99 mM $KNO_3$, 1 mM $(NH_4)_2SO_4$, 2 mM $NaH_2PO_4{\cdot}2H_2O$, 2 mM $CaCl_2{\cdot}2H_2O$ and 1 mM $MgSO_4{\cdot}7H_2O$. From these results, we determined the more upgraded culture condition for industrial production of salidroside.

대량 원소의 종류 및 적정 농도의 규명은 식물의 생장뿐 아니라 유용한 2차 대사산물의 생합성량 증가를 위해 필수적이다. 참돌꽃 배양조직에서 salidroside의 생산성을 높이기 위해 기본 배지의 대량 원소의 농도를 달리하였을 때 $KNO_3$는 98.92 mM, $(NH_4)_2SO_4$는 1.01 mM, $NaH_2PO_4{\cdot}2H_2O$는 2.18 mM, $CaCl_2{\cdot}2H_2O$는 2.04 mM, 그리고 $MgSO_4{\cdot}7H_2O$는 1.01 mM 일 때, salidroside의 생산량이 가장 높았다. 또한 이와 같은 무기이온의 농도를 조합한 $S4B_5$배지에서 기본배지인 $2XB_5$배지에 비해 2.5배 salidroside 함량 증가를 확인할 수 있었다.

Keywords

References

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