Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
권호 표지

Analysis of the Adsorbed Plasma Proteins in the Moving Actuator type Total Artificial Heart

  • Gyu Ha Ryu (Department of Biomedical Engineering and Institute of Biomedical Engineering, Seoul National University) ;
  • Jon (Department of Biomedical Engineering and Institute of Biomedical Engineering, Seoul National University, Department of Educational Mathematics, Korea National University of Education, Polymer Chemistry Laboratory, Korea Institute of Science and Technology)
  • Published : 1993.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Plasma protein adsorption is the first event in the blood-material interaction and influenc- es subsequent platelet adhesion towards thlㅈombus formation. Thiㅈomboembolic events are strongly influenced by surface characteristics of materials and fluid dynamics inside the blood pump. In vitro flow visualizaion and an amimal experiment with the moving actuator type TAH were Performed in order to investigate fluid dynamic effects on the protein adsorption. The diffel'encl level, j of shear rate inside the ventricle Lvere determined by consid- ering the direction of the major opening of four healt valves in the implanted TAH and the visualized flow patterns as well. Each ventricle of the explanted TAH was sectionalized into 12 segments according to the shear rate level. The adsorbed protein on each segment was quantified using the ELISA method after soaking in 2% (wye)SDS/PBS for two days. Adsorbed protein layer thicknesses Itvere measured by the Immunogotd method under TEM. The SEM observation show that right ventricle (RV) , immobilized with albumin, displayed different degrees of platelet adhesion on each segment, whereas the left ventricle (LV), grafted by PEO-sulronate, indicated nearly , iame platelet adhesion behavior, regardless of shear rates. The surface concentrations of adsorbed proteins in the low shear rate region are hlghel'than those in the high region, which was confirmed statistically. A modified adsorption model of plasma protein onto polyurethane surface was suggested by considering the effect of the fluid dynamic characteristics.

Keywords

References

  1. J Biomed Mater Res v.9 Surface and bulk characteristics of a polyether urethane for artificial hearts Boretos,J.W.;Pierce,W.S.;Baiier;R.E.;Leroy,A.F.;Donachy,H.J.
  2. Trans Am Soc Artif Intern Organs v.XXX The blood interface with segmented polyurethanes: Multilayered protein passivation mechanism Matsuda,T.;Takano,H.;Hayashi,K.;Taenaka,Y.;Takaichi,S.;Umezu,M.;Nakamura,T.;Iwata,H.;Nakatain,T.;Takatain,S.;Akutsu,T.
  3. Trans Am Soc Artif Intern Organs v.XXXV no.3 Scanning and transmission electron microscopic evaluation of the U-100 total artificial heart blood contacting surface Nojiri,C.;Kim,S.W.;Jacobs,H.A.;Pantalos,G.M.;Olsen,D.B.
  4. XVIII ESAO Congress A quantitative study of adsorbed plasa proteins on artificial heart blood pumps- The effect of pumping duration and coating materials Mabuchi,K.;Nakamura,H.;Imachi,K.;Chinzei,T.;Abe,Y.;Yonezawa,T.;Sugawara,I.;Fujimasa,I.
  5. IEEE trans of BME v.37 no.12 A moving actuator type electromechanical total artificial heart - Part Ⅰ: Linear type and mock circulation experiments Min,B.G.;Kim,H.C.;Lee,S.H.;Kim,J.;Kim,J.T.;Kim,I.Y.;Kim,S.W.;Diegel,P.D.;Olsen,D.B.
  6. IEEE trans of BME v.37 no.12 A moving actuator type electro-mechanical total artificial heart - Part Ⅱ: Circular type and animal experiment Min,B.G.;Kim,H.C.;Choi,J.W.;Ryu,G.H.;Seo,K.P.;Rho,J.R.;Ahn,H.;Kim,S.W.;Diegel,P.D.;Olsen,D.B.
  7. J Biomed Mater Res v.25 Negative cilia concept for thrombore-sistance: synergistic effect of PEO and sulfonate groups grafted onto polyurethanes Han,D.K.;Jeong,S.Y.;Min,B.G.;Cho,H.I.;Kim,Y.H.
  8. Trans Am Soc Artif Intern Organs, this issue Albumin-immobilized polyurethane and its blood compatibility Ryu,G.H.;Han,D.K.;Kim,Y.H.;Min,B.G.
  9. J Biomed mater Res v.24 Blood compatibility of PEO grafted polyurethane and HEMA/styrene block copolymer surfaces Nojiri,C.;Okano,T.;Jacobs,H.A.;Park,K.D.;Mohammad,S.F.;Olsen,D.B.;Kim,S.W.
  10. Scanning Microsc v.1 Surface characterization of biomaterials by immunogold staining-quantitative analysis Park,K.;Simmoons,S.R.;Albrecht,R.M.
  11. Biochem et biophys acta v.251 Enzyme-linked immunosorbent assay Engvall,E.;Jonsson,K.;Perlmann
  12. J Biomech. v.17 no.4 The hemodynamic forces acting on thrombi, from incipient attachment of single cells to mayutory and embo-lization Basmaddjin,D.
  13. Bio-materials v.7 FTIR-ATR studies of the effect of shear rate upon albumin adsorption onto polyurethaneurea Pitt,W.G.;Cooper,S.L.