• 제목/요약/키워드: light-limiting conditions

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Role of OrfQ in Formation of Light-Harvesting Complex of Rhodobacter sphaeroides under Light-Limiting Photoheterotrophic Conditions

  • LIM, SOO-KYONG;IL HAN LEE;KUN-SOO KIM;JEONG KUG LEE
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.604-612
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    • 1999
  • A puc-deleted cell of Rhodobacter sphaeroides grows with a doubling time longer than 160 h under light-limiting photoheterotrophic (3 Watts [W]/㎡) conditions due to an absence of the peripheral light-harvesting B800-850 complex. A spontaneous fast-growing mutant, R. sphaeroides SK101, was isolated from the puc-deleted cells cultured photoheterotrophically at 3 W/㎡. This mutant grew with an approximately 40-h doubling time. The growth of the mutant, however, was indistinguishable from its parental strain during photoheterotrophic growth at 10 W/㎡ as well as during aerobic growth. The membrane of SK101 grown aerobically did not reveal the presence of any spectral complex, while the amounts of the B875 complex and photosynthetic pigments of SK101 grown anaerobiclly in the dark with dimethylsulfoxide (DMSO) were the same as those of the parental cell. These results indicate that the oxygen control of the photosynthetic complex formation remained unaltered in the mutant. The B875 complex of SK101 under light-limiting conditions was elevated by 20% to 30% compared with that of the parental cell, which reflected the parallel increase of the bacteriochlorophyll and carotenoid contents of the mutant. When the puc was restored in SK101, the B875 complex level remained unchanged, but that of the B800-850 complex increased. The mutated phenotype of SK101 was complemented with orfQ encoding a putative bacteriochlorophyll-mobilizing protein. Accordingly, it is proposed that the mutated OrfQ of SK101 should have an altered affinity towards the assembly factor specific to the most peripheral light-harvesting complex, which could be either the B875 or the B800-850 complex.

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Characteristics of a Rhodobacter sphaeroides mutant selected by increased growth rate under light-limiting photoheterotrophic conditions

  • Lim, Soo-Kyoung;Lee, Il-Han;Lee, Jeong K.
    • Proceedings of the Botanical Society of Korea Conference
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    • 1999.08a
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    • pp.55-67
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    • 1999
  • A puc -deleted cell of Rhodobacter sphaeroides grows with a doubling time longer than 160 h under the light-limiting photoheterotrophic ( 3 Watts [W]/㎡) conditions due to an absence of the peripheral light-harvesting B800-850 complex. A spontaneous fast-growing mutant, R.sphaeroides SK101 was ioslate dto have∼40-h doubling at 3 Watts/㎡, while the growth of the mutant was not distinguished from its parental strain during both aerobic and light-saturating photoheterotrphic (10W/㎡) growth. The B875 complex of SK101 under the light-limiting conditions was elevated by 20 to 30% compared with that of the puc -deleted cell, reflecting parallel increase of bacteriochlorophyll and carotenoid contents of the mutant. The formation of B875 complex of SK101 under the anaerobic dark conditions with dimethylsulfoxide was the same as that of the puc-deleted cell. suggesting that the mutation of SK101 result in the altered control of B875 complex formation by light. When puc is restored in SK101 , it is not B875 complex but B800-850 complex which formation is elevated. The mutation of SK101 affected the bchF transcription most drastically to show two to tenfold increase during both aerobic and photoheterotrophic growth. The mutated phenotype of SK101 was complemented with pW2, which contains approximately 100-kb HNA of the photosynthetic gene clusters. The complementing DNA was narrowed down to a 1.1-kb DNA containing orfQ and pufKBA . The mutation of SK101 appeared to be exerted through the mutation of the orfQ gene encoding a putative bacteriochlorophyll -mobilizing protein.

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Simple Monodimensional Model for Linear Growth Rate of Photosynthetic Microorganisms in Flat-Plate Photobioreactors

  • Kim, Nag-Jong;Suh, In-Soo;Hur, Byung-Ki;Lee, Choul-Gyun
    • Journal of Microbiology and Biotechnology
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    • v.12 no.6
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    • pp.962-971
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    • 2002
  • The current study proposes a simple monodimensional model to estimate the linear growth rate of photosynthetic microorganisms in flat-plate photobioreactors (FPPBRs) during batch cultivation. As a model microorganism, Chlorella kessleri was cultivated photoautotrophically in FPPBRs using light-emitting diodes (LEDs) as the light sources to provide unidirectional irradiation in the photobioreactors. Various conditions were simulated by adjusting both the intensity of the light and the height of the culture. The validity of the proposed model was examined by comparing the linear growth rates measured with the predicted ones obtained from the proposed model. Accordingly, the value of $\frac{K\cdot\mu m}{\alpha\cdot L}log(I_0\cdot{I_s}^{\varepsilon 1)\cdot {I_c}^{-\varepsilon})$ was proposed as an approximate index for strategies to obtain the maximal lightn yield under light-limiting conditions for high-density algal cultures and as a control parameter to improve the photosynthetic productivity and efficiency.

Effect of Light, Temperature, and Shaking Speed on Production of Capsaicin in Suspension-Cultured Jalapeno Pepper (Capsicum annuum L.)

  • Lee, Kwon-Bok;Engler, Cady;Yang, Jae E.;Lee, Shin-Woo;Park, Yong-Ha
    • Journal of Applied Biological Chemistry
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    • v.44 no.2
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    • pp.84-86
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    • 2001
  • Capsaicin synthesis by suspension cultured cells of Jalapeno pepper (Capcicum annuum L.) was assessed in vitro under various conditions including temperature (23 and $30^{\circ}C$), light intensity (with light and without light), and shaking speed (110 and 200 rpm). Capsaicin production increased, while the cell biomass growth decreased possibly due to the production of a secondary metabolite. Capsaicin synthesis was primarily affected by light condition. Cells cultivated at 110 rpm and $23^{\circ}C$ under light condition yielded the highest fresh weight, while those cultivated under the same condition, but without light resulted in the lowest cell mass. Capsaicin content in cells of 18-day-old pepper grown at 110 rpm and $23^{\circ}C$ under light was 0.125% of the cell mass. However, without light treatment, the capsaicin content in cells at the same shaking speed and temperature increased up to 169%, indicating no light is favored in the capsaicin synthesis by Jalapeno pepper. Increasing the shaking speed from 110 to 200 rpm without light enhanced the capsaicin synthesis. Results of this study demonstrate that light condition is the limiting factor in the synthesis of capsaicin in tissue-cultured Jalapeno pepper cells.

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A Study on Expression Characteristics of Light in Interior Space based on Perception and Cognition (지각과 인지를 바탕으로 한 실내공간의 빛 표현특성에 관한 연구)

  • Oh, Seung-Nam;Kim, Jong-Jin
    • Korean Institute of Interior Design Journal
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    • v.15 no.6 s.59
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    • pp.247-254
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    • 2006
  • Apparently light does exist even if it does not have certain form that an object might have. The history of architecture can be told as the history of light. As various master architects as well as scholars mentioned, light has been recognized as one of the most important aspects in perceiving space. Although there are complex multi dimensions in body sense and perception, experiencing space through light is preliminary formed by the outline to the degree of brightness. Light gives space specific and unique character by limiting or enlarging the space by wrapping an object or by reflection of material surfaces or by the intensity of light itself. It is fundamentally important to recognize the essential relationship between light and perception of space through various medias such as material, form, movement and so on. Three types of space perception and cognition by light that have been categorized after studying the most significant writings on light become the core method to further analyze the selected case projects. In this research, we will study how interior space is expressed through different light conditions with above mentioned characteristics and how such expressions can be applicable to the design of interior space.

Electrochemically Fabricated Alloys and Semiconductors Containing Indium

  • Chung, Yonghwa;Lee, Chi-Woo
    • Journal of Electrochemical Science and Technology
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    • v.3 no.3
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    • pp.95-115
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    • 2012
  • Although indium (In) is not an abundant element, the use of indium is expected to grow, especially as applied to copper-indium-(gallium)-selenide (CI(G)S) solar cells. In future when CIGS solar cells will be used extensively, the available amount of indium could be a limiting factor, unless a synthetic technique of efficiently utilizing the element is developed. Current vacuum techniques inherently produce a significant loss of In during the synthetic process, while electrodeposition exploits nearly 100% of the In, with little loss of the material. Thus, an electrochemical process will be the method of choice to produce alloys of In once the proper conditions are designed. In this review, we examine the electrochemical processes of electrodeposition in the synthesis of indium alloys. We focus on the conditions under which alloys are electrodeposited and on the factors that can affect the composition or properties of alloys. The knowledge is to facilitate the development of electrochemical means of efficiently using this relatively rare element to synthesize valuable materials, for applications such as solar cells and light-emitting devices.

Compensatory growth under leaf damage of herbal vine Aristolochia contorta depends on the light availability

  • Si-Hyun Park;Bo Eun Nam;Jae Geun Kim
    • Journal of Ecology and Environment
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    • v.48 no.3
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    • pp.374-381
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    • 2024
  • Background: There is a wide range of phenotypic plasticity in plants that respond to tissue damage. Compensatory growth after physical damage may function as a part of tolerance to herbivory, which is affected by resource limitations and/or damage properties. Results: Under different light availability (unshaded and shaded) and damaged leaf ontogeny (control, young leaf- and mature leaf-damaged), compensatory growth was examined for the herbal vine Aristolochia contorta. Under the unshaded treatment, compensatory growth on leaf and branch emergence was strongly induced compared to the shaded treatment. Damage to young leaves induced leaf emergence more strongly than damage to old leaves. Conclusions: It appears that light availability acted as a limiting factor in the compensatory growth of A. contorta after the damage despite its vigorous growth under the shade treatment. Under the shade, leaf damage led to altered biomass allocation as indicated by a decrease in specific leaf area and an increase in root mass fraction. The present study contributes to the understanding of the phenotypic plasticity of vine species under different environmental conditions and damaged tissue, which may differ depending on the species' habitat range.

A study on Electromagnetic Propertie of the Ring-shaped electrodeless fluorescent lamp (동근형 무전극 형광램프의 전자계 특성)

  • Lee, Seong-Jin;Kim, Nam-Kun;Park, No-Jun;Lee, Jong-Chan;Jung, Young-II;Park, Dae-Hee
    • Proceedings of the KIEE Conference
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    • 2006.10a
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    • pp.220-221
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    • 2006
  • In recent, there have been several developments in lamp technology that promise savings in electrical power consumption and improved Quality of the lighting space. Above all, the advantages of ring-shaped electrodeless fluorescent lamp are the removal of internal electrodes and heating filaments that are a light-limiting factor of conventional fluorescent lamps. Therefore, the life time of ring-shaped electrodeless fluorescent lamps is substantially higher than that of conventional fluorescent lamps last up to 60,000 hours, There are intended as a highly efficient replacement for the incandescent reflector lamp in many applications. In this paper, maxwell 3D finite element analysis program (Ansoft) was used to obtain electromagnetic properties associated with the coil and nearby structures. The electromagnetic emitting properties were presented by 3D simulation software operated at 250 kHz and some specific conditions. In addition, the optical characteristics of ring-shaped electrodeless fluorescent lamp were measured including light flux, efficiency and color temperature for each case.

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Electromagnetic Radiation Properties of Electrodeless Fluorescent Lamps (무전극 형광램프의 전자계 방사 특성)

  • Lee, Jong-Chan;Park, Dae-Hee;Kim, Kwang-Soo;Ham, Hum;Park, Sung-Mok
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.07b
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    • pp.931-934
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    • 2002
  • In recent year there have been several developments in lamp technology that promise savings in electrical power consumption and improved quality of the lighting space. Above all, the advantage of Electrodeless fluorescent lamp is the removal of internal electrodes and heating filaments that are a light-limiting factor of conventional fluorescent lamps. Therefore, the life of Electrodeless fluorescent lamps is substantially higher than that of conventional fluorescent lamps and last up to 100,000 hours. In this paper, the Electromagnetic emitting properties were presented by simulation software operated at 2.65MHz and some specific conditions.

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Analysis of Optical and Electromagnetic Distribution of Ring-shaped Electrodeless Fluorescent Lamps (환형 무전극 램프의 광학적, 전자계적 해석)

  • 조주웅;최용성;김용갑;박대희
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.17 no.4
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    • pp.460-464
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    • 2004
  • In recent, there have been several developments in lamp technology that promise savings in electrical power consumption and improved quality of the lighting space. Above all, the advantage of ring-shaped electrodeless fluorescent lamp is the removal of internal electrodes and heating filaments that are a light-limiting factor of conventional fluorescent lamps. Therefore, the life time of ring-shaped electrodeless fluorescent lamps is substantially higher than that of conventional fluorescent lamps and last up to 60,000 hours and is intended as a high efficacy replacement for the incandescent reflector lamp in many applications. In this paper, maxwell 3D finite element analysis program (Ansoft) was used to obtain electromagnetic properties associated with the coil and nearby structures. The electromagnetic emitting properties were presented by D simulation software operated at 250KHz and some specific conditions. Photometric characteristic of the ring-shaped electrodeless fluorescent lamp were investigated using LS-100 lightmeter and TA-0510 thermometer respectively.