• Food Science and Biotechnology
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• v.17 no.3
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• pp.569-572
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• 2008

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-FIR producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

• Journal of Oriental Neuropsychiatry
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• v.11 no.1
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• pp.47-57
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• 2000

This study was designed and performed to identify the effects of oriental medical therapy on hwabyung patients by investigating the life events as the cause of Hwabyung, recent stress perception which influences the clinical appearances and change of symptoms by means of objective scales. 1. Through life events rating scale, life events are reported as follows, in the order of majority. Health, discord within family, martial life, friendship and interest, religion etc. Hwabyung patients experienced negative stress much more than positive stress. 2. Through the GARS (Global Assessment of Recent Stress), there were some significant decreases of stress perception in stress4(stress of sickness or injury), stress7(stress of change or no change in routine) and stress8(overall global stress)(p<0.05, respectively), but there were no significant decreases in five items out of eight items. 3. There were significant decreases of all rated symptoms(feeling of chest oppressed, stuffy in the chest or epigastric mass, feeling of something pushing-up, flushing of body or face, abrupt impulsion of anger, resentment), which were checked weekly for 4 times (p<0.05, respectively). In this study the symptoms of hwabyung were improved regardless of stress perception. It means that hwabyung can be improved significantly with oriental medical therapy.

• Food Science and Biotechnology
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• v.15 no.1
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• pp.148-151
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• 2006

Multiplex PCR was performed to simultaneously detect eight different events of genetically modified (GM) maize. Specific primers were constructed from GA21, T25, TC1507, Mon810, Mon863, Event176, Bt11, and NK603 events of GM maize. Using this PCR method, specific GM maize was monitored in commercialized foods and feed.

• Food Science and Biotechnology
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• v.18 no.3
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• pp.694-699
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• 2009

A multiplex polymerase chain reaction (PCR) method was developed for the detection of 4 events of genetically modified (GM) soybean. The event-specific primers were designed from 4 events of GM soybean (RRS, A2704-12, DP356043-5, and MON89788). The lectin was used as an endogenous reference gene of soybean in the PCR detection. The primer pair YjLec-4-F/R producing 100 bp amplicon was used to amplify the lectin gene and no amplified product was observed in any of the 9 different plants used as templates. This multiplex PCR method allowed for the detection of event-specific targets in a genomic DNA mixture of up to 1% GM soybean mixture containing RRS, A2704-12, DP356043-5, and MON89788. In this study, 20 soybean products obtained from commercial food markets were analyzed by the multiplex PCR. As a result, 6 samples contained RRS. These results indicate that this multiplex PCR method could be a useful tool for monitoring GM soybean.

• The Korean Nurse
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• v.25 no.1 s.134
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• pp.85-101
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• 1986

This study on stressful life events and coping methods of peptic ulcer patients and non-peptic ulcer peoples was applied to people who was divided into two groups from Oct. 28 to Nov. 16, 1985. One is patients who were conformed by gastroscopy in medical

• Journal of Korean Academy of Nursing
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• v.34 no.6
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• pp.903-912
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• 2004

Purpose: A structural equation model was analysed to explore the determinants of health behaviors of university students in Korea. Method: Nine hundred sixty nine university students were selected by random cluster sampling from five universities located in the central area of Korea. Data collection: The data was collected by questionnaires about demographic characteristics, stressful life events, perceived social support, perceived health status and health behaviors. Results: 1. Gender showed indirect effect on health behaviors. 2. Living together with(out) family had a direct effecton health behaviors: students living with family showed more positive health behaviors. 3. Stressful life events had an indirect effect on health behaviors via perceived health status; a higher score of stressful life events was the predictor for negative health behaviors. 4. A higher score of perceived health status predicted positive health behaviors. Recommendation: Each university should be encouraged to develop a health behavior control program and health promotion program for their own university students. It would be more effective to develop health programs separately according to the demographic or social characteristics of the students. It is also necessary for the Ministry of Education to reform the School Health Act and school health policy to strengthen a health promotion program for university students. In conclusion, following studies should identify and promote the validity and reliability of perceived health status and health behaviors measurements.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.34 no.4
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• pp.5-27
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• 2023

Gagné proposed nine instructional events, gain attention of the students, inform students of the objectives, stimulate recall of prior learning, present the content, provide learning guidance, elicit performance(practice), provide feedback, assess performance, enhance retention and transfer for effective teaching & learning practice. Gagné's theory is widely applied in various subjects because it increases student participation and allows classes to be developed systematically. The purpose of this study is to develop a lesson plans for the 'library and information life' subjects in middle school using Gagné's nine instructional events theory, conduct actual instructions, and verify its effectiveness. The research procedure was conducted as follows. Development of lesson plan that using Gagné's theory to Section III (Information Analysis and Interpretation) of 'Library and Information Life' in middle school. Actual instruction were conducted and student survey, peer teacher assessment, and instructor self-assessment were conducted. Based on the evaluation results, a elements to revise and improve the lesson plan was presented.

• Journal of Korean Academy of Nursing
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• v.23 no.2
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• pp.299-315
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• 1993

Koreans are one of the fastest growing immigrant groups in America. Stress responses and stressors among this large cultural minority has been rarely been studied by nursing researchers. Adjusting to life in foreign country produces a great deal of stress. Differences in culture, language, expectations and social behavior can lead to misunderstandings between health care providers and clients. These misunderstandings are not well accounted for in health assessment. This study investigated the relationship between life events or / and daily activities as a stressor and the symptoms of stress among a sample of Korean immigrants in America. The symptoms of stress scale (SOS) was used to identify stress responses and open-ended questions were used to identify life events and daily activities considered by the respondents to be stressful. A simple random sample of 283 subjects was selected from the Directory of the Korean Society of Chicago, New York, Los Angeles, Philadelphia and Seattle. Demographically, the subjects ranged in age from 20 to 69 years, and the percentage of women and men was approximately 50% each. Almost ninety percent of the subjects were highly educated, 17% owners of business, 19% white collar professionals, 14% employed in sales or as skilled /unskilled labor, 27% as housewives and students and 3％ had no occupation. The total group SOS mean was 0.8042 ; the SOS men for man was 0.7371, and for women was 0.8713. The stress response of this subject group was high, -the stress response of women higher than that for men. In an earlier study(June, 1992) with another sample, the total mean SOS score was similar to this one. The main stressful life events or / and daily activities were, in order, economic problems (N=97), interpersonal problems (N=68), children care problems (N=258), health problems (N=49), communication problems (N=42), family problems (N=38), worry about future career (N=36), and religious problems (N=25). There was a significant difference in the SOS means between the group that expressed life events or / and daily activities to be stressful and the group that did not. Interpersonal relationships and economic and family problems were stressors for those who complained about peripheral manifestations. cardiopulmonary symptoms, central-neurological symptoms, gastrointestinal symptoms, muscle ten-sion, habitual patterns, depression, anxiety, emotional irritability and cognitive disorganization. In summary, interpersonal relationships and economic and family problems influenced stress response manifestations. Income, the number of people in the family, the year of immigration. the level of education, and marital status were related to physiological and psychosocial stress responses.

• Journal of Korean Academy of Nursing
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• v.38 no.2
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• pp.217-228
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• 2008

Purpose: This study was conducted to develop and to determine the effects of an integrated symptom management program for prevention of recurrent cardiac events after percutaneous coronary intervention. Methods: Subjects consisted of 58 CAD patients (experimental group: 30, control group: 28). The experimental group participated in an integrated symptom management program for 6 months which was composed of tailored education, stress management, exercise, diet, deep breathing, music therapy, periodical telephone monitoring and a daily log. The control group received the usual care. Results: The experimental group significantly decreased symptom experiences and the level of LDL compared to the control group. The experimental group significantly increased self care activity and quality of life compared to the control group. Although no significant difference was found in cardiac recurrence, the experimental group had fewer recurrences. Conclusion: These results suggest that an integrated symptom management program for prevention of recurrent cardiac events after percutaneous coronary intervention can improve symptom aggravation, recurrent rate, self care activity and quality of life. Nursing interventions are needed to maintain and further enhance the quality of life of these patients and the interventions should be implemented in the overall transition period.

• Journal of the Korean housing association
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• v.14 no.2
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• pp.121-132
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• 2003

This study has been performed to explore main reasons for staying in paid elderly residential care facilities among the residents. In this study, five males and seven females aged 65 to 82 were asked about main reasons for staying in the facilities. The answers of the qualitative interview were drawn as follows; first, some residents had suddenly decided to move into the facility due to critical life events such as bereavement, serious illness, or supporting problems. Second, some residents had taken a long period of considerations on whether staying home or in the facilities, because their health and family supports gradually diminished over the long life span. On the other hand, a few of them voluntarily chose to stay at the facilities, because they recognized supervised group activities, nutritionally well-planned meals, and health care as major inconveniences. In this study, suggestions were made as follows: first, guiding and settlement programs should be developed for the residents of the sudden events, particularly at early stage of admission. Second, intensive nursing care units should be in separation from healthy independent groups, so that the residents are relieved from stressful contacts with extremely ill patients in the residential area.