Detection of Eight Different Events of Genetically Modified Maize by Multiplex PCR Method

  • Kim, Jae-Hwan (Institute of Life Sciences and Resources and Department of Food Science & Biotechnology, Kyung Hee University) ;
  • Song, Hee-Sung (Institute of Life Sciences and Resources and Department of Food Science & Biotechnology, Kyung Hee University) ;
  • Heo, Mun-Seok (Institute of Life Sciences and Resources and Department of Food Science & Biotechnology, Kyung Hee University) ;
  • Lee, Woo-Young (Nutrition Evaluation Team, Korea Food and Drug Administration) ;
  • Lee, Soon-Ho (Nutrition Evaluation Team, Korea Food and Drug Administration) ;
  • Park, Sun-Hee (Nutrition Evaluation Team, Korea Food and Drug Administration) ;
  • Park, Hye-Kyung (Nutrition Evaluation Team, Korea Food and Drug Administration) ;
  • Kim, Myung-Chul (Nutrition Evaluation Team, Korea Food and Drug Administration) ;
  • Kim, Hae-Yeong (Institute of Life Sciences and Resources and Department of Food Science & Biotechnology, Kyung Hee University)
  • Published : 2006.02.28

Abstract

Multiplex PCR was performed to simultaneously detect eight different events of genetically modified (GM) maize. Specific primers were constructed from GA21, T25, TC1507, Mon810, Mon863, Event176, Bt11, and NK603 events of GM maize. Using this PCR method, specific GM maize was monitored in commercialized foods and feed.

Keywords

References

  1. Clive J. Preview: Global status of commercialized biotech/GM crops. ISAAA briefs no. 32, ISAAA: Ithaca, NY, USA (2004)
  2. Heo MS, Kim JH, Shin WS, Park SH, Park HK, Kim MC, Kim HY. Limits of detection for genetically modified soybean in Doenjang (Korean fermented soypaste). Food Sci. Biotechnol. 13: 657-661 (2004)
  3. Vollenhofer S, Burg K, Schmidt J, Kroath H. Genetically modified organisms in food-screening and specific detection by polymerase chain reaction. J. Agric. Food Chem. 47: 5038-5043 (1999) https://doi.org/10.1021/jf990353l
  4. Permingeat, HR, Reggiardo MI, Valejos RH. Detection and quantification of transgenes in grains by multiplex and real-time PCR. J. Agric. Food Chem. 50: 4431-4436 (2002) https://doi.org/10.1021/jf020081d
  5. Yamaguchi H, Sasaki K, Umetsu H, Kamada H Two detection methods of genetically modified maize and the state of its import into Japan. Food Control 14: 201-206 (2003) https://doi.org/10.1016/S0956-7135(02)00062-2
  6. Duijn G, Biert R, Bleeker-Marcelis H, Boeijen I, Adan AJ, Jhakrie S, Hessing M. Detection of genetically modified organisms in foods by protein- and DNA-based techniques: bridging the methods. J. AOAC Int. 85: 787-791 (2002)
  7. Matsuoka T, Kuribara H, Akiyama H, Miura H, Goda Y, Kusakabe Y, Kenji I, Toyoda M, Hino A. A multiplex PCR method of detecting recombinant DNAs from five lines of genetically modified maize. J. Food Hyg. Soc. Japan 42: 24-32 (2001) https://doi.org/10.3358/shokueishi.42.24
  8. Kim YM, Sohn SH, Jeong SI, Yoon MS, Kim TS, Park YH. Detection methods for genetically modified soybeans. J. Korean Soc. Agric. Chem. Biotechnol. 45: 185-189 (2002)
  9. Seo HW, Yi JY, Cho HM, Kim SY. Primer for the potato specific internal control DNA and screening method for the genetically modified potatoes by competitive duplex-PCR. Korean J. Plant Biotechnol. 29: 235-240 (2002)
  10. Shin DW, Park SH, Woo GJ, Kim HY, Park CS. Case study for natural gene transfer from genetically modified food to food microorganisms. Food Sci. Biotechnol. 13: 342-346 (2004)
  11. Ceron J, Covarrubias L, Quintero R, Ortiz A, Ortiz M, Aranda E, Lina L, Bravo A. PCR analysis of the cryl insecticidal crystal family genes from Bacillus thuringiensis. Appl. Environ. Microbiol. 60: 353-356 (1994)
  12. Matsuoka M, Minami E. Complete structure of the gene for phosphoenolpyruvate carboxylase from maize. Eur. J. Biochem. 181: 593-598 (1989) https://doi.org/10.1111/j.1432-1033.1989.tb14765.x
  13. Klee HJ, Muskopf YM, Gasser CS. Cloning of an Arabidopsis thaliana gene encoding 5-enolpyruvylshikimate-3-phosphate synthase: sequence analysis and manipulation to obtain glyphosate-tolerant plants. Mol. Gen. Genet. 210: 437-432 (1987) https://doi.org/10.1007/BF00327194
  14. Matsuoka T, Kawashima Y, Akiyama H, Miura H, Goda Y, Kusakabe Y, Toyoda M, Hino A. A method of detecting recombinant DNAs from four lines of genetically modified maize. J. Food Hyg. Soc. Japan 41: 137-143 (2000) https://doi.org/10.3358/shokueishi.41.137
  15. Kuribara H, Shindo Y, Matsuoka T, Takubo K, Futo S, Aoki N, Hirao T, Akiyama H, Goda Y, Toyoda M, Hino A. A novel reference molecules for quantitation of genetically modified maize and soybean. J. AOAC Int. 85: 1077-1089 (2002)
  16. Kirihara JA, Petri JB, Messing J. Isolation and sequence of a gene encoding a methionine-rich 10-kDa zein protein from maize. Gene 71: 359-370 (1988) https://doi.org/10.1016/0378-1119(88)90053-4
  17. Bertheau Y, Diolez A, Kobilinsky A, Magin K. Detection methods and performance criteria for genetically modified organisms. J. AOAC lnt. 85: 801-808 (2002)
  18. Heo MS, Kim JH, Park SH, Woo GJ, Kim HY. Detection of genetically modified maize by multiplex PCR method. J. Microbiol. Biotechnol. 14: 1150-1156 (2004)