• Title/Summary/Keyword: laboratory activities

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Epidemiological Observation on the Current Epidemic of Human Trypanosomiasis in Uganda (우간다의 Human Trypanosomiasis 대유행(大流行)에 관한 역학적(疫學的) 관찰(觀察))

  • Chu, He-Len;Rim, Hang-Jong;Chu, I.H.;Ongom, V.L.
    • Journal of agricultural medicine and community health
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    • v.5 no.1
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    • pp.5-15
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    • 1980
  • The first recorded trypanosomiasis epidemic in Uganda took place at the beginning of this century in the islands and in a strip along the northern shores of Lake Victoria, which resulted in deaths of 1/3 million people. The disease was partly controlled by early 1930's and continued to occur sporadically in certain localized foci. The disease has however flared up in an explosive outbreak in Busoga district along Lake Victoria since 1977. The incidence of disease in northern district adjacent to Southern Sudan is also increasing lately. This paper describes the three month observation on the surveillance and control activities in the epidemic areas and of various health units including the Vector Control Division, the Tsetse fly Control Division, Tororo Trypanosomiasis Research Institute, medical units in Busoga, and Acholi districts. Data analysis and review were made of disease information so far collected by various health units in the Ministry of Health and district health offices. The findings may be summarized in the following: 1) A total of 12, 100 patients and 38 deaths: have occured in Busoga district since 1977 onward, and over 100 cases of diseases arc occuring in the Northern region bordering Southern Sudan. 2) the distribution of trypanosomiasis is characterized with two district patterns. The disease caused by Trypanosoma rhodesiense occurs in Busoga and is transmitted by Glossina palpalis, G. fuscipes infested in the islands and in the northern shore of forests of Lake Victoria. Another type caused by Trypanosoma gambiense occurs in Madi and Acholi in the north and is transmitted by Glossina morsitans in Savannah. 3) The house survey in Rusoga indicated that most of patients keep domestic animals in their house premises, and are engaging in either farming or fishing. Practically all the patients remembered that they had been bitten by tsetse in the field. 4) The routine diagnostic methods in the hospital laboratory is carried out through the microscopic examination of trypanosome with Giemsa stain of blood and cerebro-spinal fluid, The measurement of ESR and IgM has been used by Tororo Tryponosomiasis Research Institute for field screening.

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Pre Service Chemistry Teachers' Understanding of Science Practices During Open-inquiry Chemistry Laboratory Activities (탐구화학실험 수행에서 나타나는 예비화학교사의 탐구특성 및 변화 분석)

  • Shim, Heontae;Ryu, Suna
    • Journal of the Korean Chemical Society
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    • v.62 no.1
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    • pp.52-63
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    • 2018
  • The purpose of this study was to observe and analyze how pre-service secondary chemistry teachers conduct open-ended, free style, exploratory chemistry experiments. The study examined common difficulties and tendencies that teachers showed in the chemistry labs. The study also investigated how these teachers deal with their difficulties and obstacles, such as unexpected results and device errors. Next, the study explored how students' epistemic goals and considerations have been shaped and changed as they interacted with one another and participated in their experiments over time. The study suggests that it could be more effective to gradually decrease guided-features of the experiment by providing appropriate scaffolding, than to start with completely open-ended inquiry labs. The study also suggests that the establishment of epistemic agency among group members influences the way they conduct experiments. Consequently, it is necessary to consider how instructional design for open-ended inquiry labs may provide students with scaffolds to encourage their active participation and to build responsibility in group discussions.

Temporal Change in Radiological Environments on Land after the Fukushima Daiichi Nuclear Power Plant Accident

  • Saito, Kimiaki;Mikami, Satoshi;Andoh, Masaki;Matsuda, Norihiro;Kinase, Sakae;Tsuda, Shuichi;Sato, Tetsuro;Seki, Akiyuki;Sanada, Yukihisa;Wainwright-Murakami, Haruko;Yoshimura, Kazuya;Takemiya, Hiroshi;Takahashi, Junko;Kato, Hiroaki;Onda, Yuichi
    • Journal of Radiation Protection and Research
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    • v.44 no.4
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    • pp.128-148
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    • 2019
  • Massive environmental monitoring has been conducted continuously since the Fukushima Daiichi Nuclear Power accident in March of 2011 by different monitoring methods that have different features together with migration studies of radiocesium in diverse environments. These results have clarified the characteristics of radiological environments and their temporal change around the Fukushima site. At three months after the accident, multiple radionuclides including radiostrontium and plutonium were detected in many locations; and it was confirmed that radiocesium was most important from the viewpoint of long-term exposure. Radiation levels around the Fukushima site have decreased greatly over time. The decreasing trend was found to change variously according to local conditions. The air dose rates in environments related to human living have decreased faster than expected from radioactive decay by a factor of 2-3 on average; those in pure forest have decreased more closely to physical decay. The main causes of air dose rate reduction were judged to be radioactive decay, movement of radiocesium in vertical and horizontal directions, and decontamination. Land-use categories and human activities have significantly affected the reduction tendency. Difference in the air dose rate reduction trends can be explained qualitatively according to the knowledge obtained in radiocesium migration studies; whereas, the quantitative explanation for individual sites is an important future challenge. The ecological half-lives of air dose rates have been evaluated by several researchers, and a short-term half-life within 1 year was commonly observed in the studies. An empirical model for predicting air dose rate distribution was developed based on statistical analysis of an extensive car-borne survey dataset, which enabled the prediction with confidence intervals. Different types of contamination maps were integrated to better quantify the spatial data. The obtained data were used for extended studies such as for identifying the main reactor that caused the contamination of arbitrary regions and developing standard procedures for environmental measurement and sampling. Annual external exposure doses for residents who intended to return to their homes were estimated as within a few millisieverts. Different forms of environmental data and knowledge have been provided for wide spectrum of people. Diverse aspects of lessons learned from the Fukushima accident, including practical ones, must be passed on to future generations.

KOREAN PARTICIPATION ON THE COSMIC INFRARED BACKGROUND EXPERIMENT 2 (CIBER2) (적외선 우주배경복사 관측 실험 2(CIBER2) 국제 공동 연구)

  • Lee, D.H.;Park, W.K.;Moon, B.G.;Park, S.J.;Kim, M.G.;Kim, G.H.;Nam, U.W.;Pyo, J.;Jeong, W.S.;Park, Y.S.;Kim, I.J.;Han, W.
    • Publications of The Korean Astronomical Society
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    • v.30 no.1
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    • pp.11-16
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    • 2015
  • First light galaxies have predictable linear clustering, and are expected to produce fluctuations with a characteristic spatial power spectrum, which peaks at an angular scale of ~ 10 arcminutes and in the $1-2{\mu}m$ spectral regions. The Cosmic Infrared Background ExpeRiment 2 (CIBER2) is a dedicated sounding rocket mission for measuring the fluctuations in the extragalactic infrared background light, following up the previous successful measurements of CIBER1. With a 28.5 cm telescope accompanied with three arms of camera barrels and a dual broadband filter on each H2RG (${\lambda}_c=2.5{\mu}m$) array, CIBER2 can measure 6 bands of wide field ($1.1{\times}2.2$ degrees) up to 3 AB magnitudes deeper than CIBER1. This project is leaded by California Institute of Technology/Jet Propulsion Laboratory, collaborating internationally with Institute of Space and Astronautical Science in Japan, Korea Astronomy and Space Science Institute, Korea Basic Science Institute, and Seoul National University. The Korean team is in charge of 1) one H2RG scientific array, 2) ground station hardware and software, 3) telescope lenses, and 4) flight and test bed electronics fabrication. In this paper, we describe the detailed activities of the Korean participation as well as the current status of the CIBER2 project.

A Study on the Factors which influenced the Performance of Community Health Practitioners' Function -Around the CHPs in Kyonggi-province Area- (보건진료원 직무수행에 영향을 미치는 요인에 관한 연구 - 경기도 관내 보건진료원을 중심으로 -)

  • Lee Myoung-sook
    • Journal of Korean Public Health Nursing
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    • v.3 no.1
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    • pp.18-37
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    • 1989
  • This study was done in order to analyze the factors which influenced the performance level of community health practitioners' task. Interview survey was done during the period from August to October, 1986. Interviewee were 166 CHPs among total of 217 CHPs in Kyonggi province area. Multiple stepwise regression and canonical correlation analysis were used to identify major factors influenced to perform community health practitioners' task. The results of this study were summarized as follows: 1. General characteristics of CHPs 1) Personal characteristics The average age of CHPs was 37.8 years and their marital status was $77.6\%$ of married, educational back-ground was $65.3\%$ of junior college graduation. Their job career was $38.6\%$ of between 1-3 years, $33.3\%$ of between 3-5 years, $22.2\%$ of less than 1 years. Most of CHPs$(62.8\%)$ were fully satisfied with their job, $33.3\%$ were moderately, and $3.8\%$ were not satisfied. 2) Working environmental condition Only $31.7%$ of CHPs were satisfied with their working condition of primary health post, $26.6\%$ were not satisfied. Half of CHPs$(52.5\%)$ replied having good cooperation with health center, $10.1\%$ replied bad. Cooperation with health subcenter was good in $32.9\%$, and bad in $21.9%$. Cooperation with private health institutions was good in $34.2\%$, bad in $21.6%$. 2. Performance level of community health practitioners' task Among a total of 52 contents of their functions medical history taking. physical examination, referral of diagnostic laboratory work-up($(86.4\%)$, health assessment of pregnant women$(82.1\%)$, development of health information system$(79.4\%)$, supervision of health workers $(78.4\%)$, follow-up of family planning acceptors$(77.3\%)$, and follow-up of family planning acceptors' side effects$(77.3\%)$ were actively performed. Diagnosis of pregnancy$(62.1\%)$, sampling of drinking water for quality test$(52.5\%)$, making list of equipment' & supplies $(51.5\%)$, evaluation of primary health post activities $(37.6\%)$, organization of village health workers$(32.4\%)$ and management of village health workers $(30.1\%)$ were poorly performed. 3. Stepwise multiple regression analysis of job function The factors which influenced the performance level of community health practitioners' function were age, marital status, educational level, job career, job satisfaction, satisfaction of working environment of primary health post, cooperation of health center, cooperation of health center, cooperation of private health instiutions in orders. These 9 variables were able to explain job function from $25.7\%$ of program planning to $6.7\%$ of management of common disease. 4. Canonical correlation analysis between the performance of function and general characteristics of CHPs. Cooperation of private health institutions was found to be the factor influencing task performance of community organization, management of primary health post, technical supervision of health personnels. Job satisfaction of CHPs was also found to be the factor influencing task performance of family planning, management of common disease and maintenance of health information system.

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Selection of Kimchi Starters Based on the Microbial Composition of Kimchi and Their Effects (김치 미생물 조성을 바탕으로 한 김치 스타터의 선정 및 효과)

  • Jin, Hyo-Sang;Kim, Jong-Bum;Yun, Yeong-Ju;Lee, Kyung-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.5
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    • pp.671-675
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    • 2008
  • Based on information about the major microbial composition of kimchi and its relation to the taste, Leuconostoc mesenteroides K2M5 and Lactobacillus sakei K5M3 were selected as kimchi starter candidates. These two strains were found to be safe for industrial use because they showed neither harmful characteristics like ${\beta}$-hemolysis, ammonia and indole formation, and gelatin liquefaction, nor enzymatic activities like phenylalanine deaminase, ${\beta}$-glucuronidase, ${\beta}$-glucosidase, 7${\alpha}$-dehydroxylase and nitroreductase. Starter kimchi made with these strains were better in taste than the conventional kimchi when they are evaluated both by laboratory personnel and the public. Also microbial analysis of starter kimchi showed only starter bacteria after they were fermented to have the optimum acidity. Starter kimchi prepared with these two strains were not much different in physicochemical properties to the conventional kimchi except in that the starter kimchi were much higher in volatile organic acid content such as lactic acid. These results suggest that kimchi quality can be controlled to have consistent properties, both in taste and microbial composition, by using bacterial starters.

Studies on the fungicidal action and its physico-chemical properties of phenylmercuric 8-oxyquinolinate (Phenylmercuric 8-oxyquinolinate의 살균작용 및 이의 이화학적 성질에 관한 연구)

  • Sohn C. Y.;Kang I. M.;Lee S. H.
    • Korean journal of applied entomology
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    • v.4
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    • pp.11-18
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    • 1965
  • In order to investigate the fungicidal activities against various plant pathogenes, diminishing effect of plant transpiration, phytotoxicities, vapor effect and the rate of reduction by ultraviolet rays of phenylmercuric 8-oxyquinolinate(P.M.Q), this experiments were undertaken under various laboratory conditions. 1. Inhibitory activity on the spore germination of this chemical was shown less effective than that of P.M.A..(Table 2, Table 3, Table 4, Table 5 and Table 6) Also, P.M.Q. was resulted a somewhat higher inhibitory activity on the hyphae growth than P.M.A. (Table 7). 2. In the diminishing effect of plant transpiration, 8-hydroxyquinoline sulfate(oxine sulfate) was more strong inhibitory at first than P.M.Q., while, at last, P.M.Q. was more strong inhibitory in comparison with oxine sulfate(Table 8, Fig. 1 and Table 9). 3. P.M.Q. was shown less injury on the germination of rice plant seeds and the emergence of their roots than P.M. A.(Table 10). Injuries was not observed on the rice seedlings and soy-bean seedlings sprayed with 40 ppm of this chemical. 4. P.M.A. had more inhibitory effects on the mycelial growth of phytopathogenes than P.M.Q. on the vapor effect (Table 11, Fig. 2). 5. Biological activity and chemical decomposition rate of P.M.A. were greatly reduced by exposure of this compound to ultraviolet rays. But, P.M.Q. was only slightly affected by similar treatment(Table 12, Fig. 3, Table 13 and Fig. 4). From the above results, this chemical will be a promising fungicide adding fungitoxicities against various phytopatho genes, diminishing effect of plant transpiration and physico-stability.

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Pesticidal Activities and Effect on Its Biological Characteristics of Bacillus thuringiensis Strains from Soil against Rice Pests, Cnaphalocrosis medinalis and Maranga aenescens (토양에서 분리한 곤충병원성세균 Bacillus thuringiensis 의 혹명나방과 벼애나방에 대한 실내살충효과검정 및 생물학적 특성에 미치는 영향)

  • Seo, Mi-Ja;Paik, Chae-Hoon;Kang, Mi-Hyung;Lee, Geon-Hwi;Lee, Du-Ku;Lee, Kyu-Seong;Youn, Young-Nam;Yu, Yong-Man
    • Korean journal of applied entomology
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    • v.48 no.1
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    • pp.101-108
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    • 2009
  • For biological control of larva of Cnaphalocrocis medinalis and Naranga aenescens do considerable damage to crops by folding and scraping the leaf tissue of rice, a large number of Bacillus thuringiensis isolates have been obtained from soil samples in Korea and the pesticidal activity was assayed against two insect pest species described above. Among 53 Bt isolates tested in bioassay, 18 and 13 isolates showed over 90% mortality against C. medinalis and N. aenescens, respectively. Some isolates (11 isolates including CAB141) presented dual activity against C. medinalis and N. aenescens. These isolates showed over 96% control effect in pest control in laboratory against larvae of C. medinalis. Also, it was investigated that pupation, pupal length, and adult emergence of larvae exposed to Bt suspension decreased. Especially, the pupal length of C. medinalis after being fed corn seedling leaves treated Bt suspension for 10 days, were much smaller than that of control.

Effects of Long-Term Weight Training Exercise on Aging Heart in Rat (장기간 체중부하 운동훈련이 흰쥐 노화 심근에 미치는 영향)

  • 조현국;이영실;정형재;이용덕;박원학
    • Biomedical Science Letters
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    • v.4 no.2
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    • pp.87-101
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    • 1998
  • There is considerable interests in the effect of regular, vigorous exercise, in particular weight training as a possible measure in improving myocardial function. The present investigation aimed to examine possible effect of a long-term weight training program on the heart in aging rats. Male rats aged 3-,10-, and 20-month-old were divided at random into a control (sedentary) and the exercise group. The training group was exercised for 5 days a week by 1 RM of ten times with weight training apparatus. This investigation examined the changes of the heart muscle relative to histological, ultrastructural, cytochemical, and stereological studies in rats. Quantity of lipofuscin pigments was clearly increased in the weight training group of 15-month-old rats, and mitochondrial degeneration, vacuolization, and interstitial proliferation were observed as well. In the weight training group of 25-month-old rats the frequency as separated intercalated discs, fraying myofibrils and hypercontraction band increased in number compared with the same 15-mon1h-old group. From the experimental result of glucose-6-phosphatase activity, the enzyme activities decreased in the weight training group of 15-month-old rats, and more decreased in the same 25-month-old group. In stereological study, both 15- and 25-month-old training groups, mitochondrial and myofibrilar volume densities significantly decreased, whereas interstitial volume density significantly increased. From the experimental results obtained in the present study, it is suggested that long-term weight training exercise do not cause any significantly qualitative and quantitative ultrastructural change of the heart muscle in the young. On the contrary, long-term weight training exercise stress may actually induce degenerative changes in the heart muscle in the old age.

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A Study on Factors Related to Stroke Patients in Taegu Area (한방병원 내원 뇌졸중 환자에 대한 역학적 조사)

  • 김웅각
    • Biomedical Science Letters
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    • v.4 no.2
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    • pp.153-164
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    • 1998
  • A study on factors related to stroke patients in three oriental medical hospitals in Taegu area was conducted from October 1997 through December 31, 1997. One hundred and sixty three hospitalized and ambulatory patients were interviewed by traind interviewers according to the questionnaires prepared in advance. The results are summarized as follows; male and female ratio of the subjects was 1 to 1.36, and of the subjects 36.8% were 60's of the age and 25.2% were 50's and 19.6% were 70's. 74.7% of the patients were married and 41.5% of them had only six year education. 20% of the subjects became stroke victims while they were engaged in physical activities, 16.2% while in sleep, 15.5% because of psycological shock, and 7.2% while drinking. Of 157 precisely diagnosed cases, cerebral infarction was the most frequent cause (47.7%), cerebral hemorrhage was the second (38.2%), and subarachnoid hemorrhage (5.1%) respectly, Of 161 cases, 64% were normal in body weight, 26.7% were obese and 9.3% were light. Of 163 cases, 65.6% of them did not drink alcohol. Of 162 cases, 63% did not smoke cigarettes.

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