• Title/Summary/Keyword: k-mer analysis

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Secrecy Analysis of Amplify-and-Forward Relay Networks with Beamforming

  • Chen, Pu;Ouyang, Jian;Zhu, Wei-Ping
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.10 no.10
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    • pp.5049-5062
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    • 2016
  • This paper analyzes the secrecy performance of an amplify-and-forward (AF) relay network, where a multi-antenna eavesdropper attempts to overhear the transmitted message from a multi-antenna source to a multi-antenna destination with a single antenna relay. Firstly, we derive the approximate analytical expressions for the secrecy outage probability (SOP) and average secrecy rate (ASR) of the relay network. Then, asymptotic expressions of SOP and ASR at high main-to-eavesdropper ratio (MER) are also provided to reveal the diversity gain of the secure communication. Finally, numerical results are given to verify the theoretical analysis and show the effect of the number of antennas in the considered relay network.

Reducing Transmit Power and Extending Network Lifetime via User Cooperation in the Next Generation Wireless Multihop Networks

  • Catovic, Amer;Tekinay, Sirin;Otsu, Toru
    • Journal of Communications and Networks
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    • v.4 no.4
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    • pp.351-362
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    • 2002
  • In this paper, we introduce a new approach to the minimum energy routing (MER) for next generation (NG) multihop wireless networks. We remove the widely used assumption of deterministic, distance-based channel model is removed, and analyze the potentials of MER within the context of the realistic channel model, accounting for shadowing and fading. Rather than adopting the conventional unrealistic assumption of perfect power control in a distributed multihop environment, we propose to exploit inherent spatial diversity of mobile terminals (MT) in NG multihop networks and to combat fading using transmit diversity. We propose the cooperation among MTs, whereby couples of MTs cooperate with each other in order to transmit the signal using two MTs as two transmit antennas. We provide the analytical framework for the performance analysis of this scheme in terms of the feasibility and achievable transmit power reduction. Our simulation result indicate that significant gains can be achieved in terms of the reduction of total transmit power and extension of network lifetime. These gains are in the range of 20-100% for the total transmit power, and 25-90% for the network lifetime, depending on the desired error probability. We show that our analytical results provide excellent match with our simulation results. The messaging load generated by our scheme is moderate, and can be further optimized. Our approach opens the way to a new family of channel-aware routing schemes for multihopNG wireless networks in fading channels. It is particularly suitable for delivering multicast/ geocast services in these networks.

Genetic Diversity of Paecilomyces japonica and Cordypces militaris Strains by URP-PCR Fingerprinting (URP-PCR핵산지문에 의한 눈꽃동충하초 (Paecilomyces japonica.)와 번데기동충하초(Cordypces militaris) 유전적 다양성분석)

  • Kim, Jong-Kun;Kang, Hee-Wan
    • The Korean Journal of Mycology
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    • v.39 no.3
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    • pp.180-184
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    • 2011
  • This study was carried out to identify the genetic characteristics among isolates of Paecilomyces spp.and Cordyceps spp. by URP-PCR analysis. Twenty URP (universal rice primer) primers of 20 mer which were designed from repetitive sequence of rice, were used for producing PCR DNA fingerprints of the mushrooms. Of them, 5 URP primers, URP2F, URP2R, URP9F, URP4R, and URP17R amplified genomic DNA of the mushrooms with polymorphic PCR patterns. On isolates of Cordyceps militaris, primers URP1F, URP2R, URP6R and URP17R produced PCR polymorphic bands of 4 types. Isolates of Cordypces sp. that are isolated from different area of Korea were identical to isolate of C. militaris, while other species of Cordypces were different to the PCR profiles. However, the URP primers did not identify the polymorphism of PCR profile on isolates of P. japonica.

Molecular discrimination of Panax ginseng species

  • Um, Jae-Young;Chung, Hwan-Suck;Kim, Hyun-Ju;Kim, Dae-Ki;Shim, Kyung-Shik;Lee, Kang-Yong;Kim, Jeong-Sook;Choi, Tae-Jin;Kim, Nam-Song;An, Nyeon-Hyoung;Lee, Kang-Min;Lee, Young-Mi;Kim, Jeong-Joong
    • Advances in Traditional Medicine
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    • v.1 no.2
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    • pp.52-58
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    • 2000
  • In order to develop convenient and reproducible methods for identification of ginseng drugs at a DNA level, RAPD (randomly amplified polymorphic DNA) and PCR-RFLP (PCR-Restriction fragment length polymorphism) analysis were applied within Panax species. To authenticate Panax ginseng betvyeen Chinese and Korean ginseng population, RAPD analysis were carried out using 20 mer-random primer. The similarity coefficients among the DNA of ginseng plants analyzed were low, ranging from 0.197 to 0.491. In addition, using PCR-RFLP analysis, very different fingerprints were obtained within Korean ginseng plants. These results suggest that these methods are able to authenticate the concerned Panax species. Broader application of this approach to authenticate other morphologically similar medicinal materials is rationalized.

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Use of RAPD Fingerprinting for Discriminating Two Populations of Hilsa shad (Tenualosa ilisha Ham.) from Inland Rivers of Bangladesh

  • Shifat, Rehnuma;Begum, Anwara;Khan, Haseena
    • BMB Reports
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    • v.36 no.5
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    • pp.462-467
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    • 2003
  • The Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) was applied to analyze the genetic variation of the Hilsa shad, Tenualosa ilisha Ham., from the two major inland rivers (Padma and Meghna) in Bangladesh. Twenty-eight random 10-mer primers were primarily scored in 8 individuals from each of the two locations. Fifteen primers, which gave polymorphism, were selected and used in the final analysis of 34 individuals from the two sites. Using these primers, 480 scorable DNA fragments were found, of which 98 (20.41%) were polymorphic. By comparing the RAPD banding patterns, variations were found between and within the populations. A dendrogram was constructed with the polymorphic fragments to analyze the genetic distances between the Hilsa shad populations. The results show two major clusters of Padma and Meghna, assuming different spawning populations with different stocks or races of Hilsa shad in the major Bangladesh rivers.

Random Amplified Polymorphic DNA (RAPD) Variation in Porhyra yezoensis and P. tenera

  • Beom-Kyu Kim;Gyu-Hwa Chung;Yuji Fujita
    • Journal of Aquaculture
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    • v.10 no.3
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    • pp.321-326
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    • 1997
  • The random amplified polymorphic DNA (RAPD) technique was used to anlayze six isolates of two species of Porphyra, P. yezoensis and P. tenera. Four 21-mer prrmers were combined randomly into six groups of double primers and screened for DNA amplification using nuclear and chloroplast tempate DNA. The RAPD patterns resulting from RnRc and CnCc primers provided evidence for both genetically homo-and heterogeneous populations of P. yezoensis and P. tenera. Similarity values obtained by RnRc primer analysis of nuclear DNA varied from 0.364 to 0.714 and those of chloroplast DNA were high, ranging from 0.727 to 1.000, except for P. yezoensis (Enoura).

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A Genetic Marker for the Korean Native Cattle (Hanwoo) Found by an Arbitrarily Primed-Polymerase Chain Reaction (AP-PCR)

  • Lee, Ji-Seon;Lee, Chang-Hee;Nam, Doo-Hyun;Jung, Young-Ja;Yeo, Jung-Sou
    • BMB Reports
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    • v.33 no.3
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    • pp.208-212
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    • 2000
  • In order to develop a specific genetic marker for the Korean native cattle (Hanwoo), an arbitrarily-primed polymerase chain reaction (AP-PCR) analysis of 6 different cattle breeds was attempted. Eight different arbitrary primers, each longer than 20-mer nucleotides, were used. In comparison to the AP-PCR patterns, several distinctive DNA bands that are specific for a certain breed were detected. When the primer Kpn-X was employed, a 280bp DNA fragment was found to be specific only for Hanwoo. In an individual analysis of Hanwoo, this AP-PCR marker was observed in 123 head of cattle among the 153 that were tested (80.4%). Nucleotide sequencing revealed that this fragment has a short microsatellite sequence of tandem repeat, $A(G)_{1-2}\;(C)_{1-3}AGAG$. According to the analysis of AP-PCR band patterns, Hanwoo was discovered to be genetically most closely-related with Holstein among the various cattle breeds.

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Isolation and Characterization of Cryptococcus neoformans from Environmental Sources in Busan

  • Oh, Kwang-Seok;Hwang, Soo-Myung
    • Mycobiology
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    • v.33 no.4
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    • pp.188-193
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    • 2005
  • Twenty nine samples of pigeon droppings (n = 12) and soil contaminated with avian excreta (n = 19), collected from different sites in Busan, were examined for isolation and characterization of Cryptococcus neoformans. Of these samples, 5 strains of C. neoformans were recovered from pigeon droppings (5/12 : 41.7%). All isolates were belonged to C. neoformans var. grubii (serotype A). The extracellular enzyme activities of the strains by using the API-ZYM system showed two different enzymatic patterns. The genetic variability among C. neoformans isolates was analyzed by random amplified polymorphic DNA (RAPD) using three 10-mer primers. Two different RAPD patterns, which clearly distinguished the isolates, were identified. Analysis of RAPD patterns provided a good characterization of environmental strains of C. neoformans serotype A as a heterogeneous group and were in good agreement with enzymatic profiles.

Genetic relationships among penicillium species by characterizing RAPD markers

  • Yoon, Cheol-Sik;Bae, Kyung-Sook
    • Journal of Microbiology
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    • v.33 no.3
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    • pp.171-177
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    • 1995
  • Random amplified polymorphic DAN markers were characterized for three taxonomically problematic Penicillium species : P. aurantiogriseum var. Aurantiogriseum, P. verrucosum and P. puberulum, as well as for 25 species of mono, bi-, and terverticillate Penicillia. The relationships among mono, bi-, and terverticillate Penicillium species were determined from these RAPD markers. Eight species from mono-, eight from bi-, and nine from terverticilate Penicillia were examined. With 14 randomly chosen 10-mer primes, a 310 character by 25 species matrix was generated. Phenetic analysis separated the 25 species into three genetically distinct groups that correspond to the different arrangements of penicilli (mono-, bi-, and terverticillate). The results of this study suggest that P. aurantiogriseum var. aurantiogriseum, P. VERRUCOSUM, AND P. puberulum represent genetically distinct species, and that P. vulpinum should be included in terverticilate Penicillia. Phenogram branching patterns indicated that biverticillate species are genetically more similar to monoverticilate species than they are to terverticillate species.

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Whole Genome Sequencing and Gene Prediction of Cynodon transvaalensis

  • Sol Ji Lee;Chang soo Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.237-237
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    • 2022
  • Cynodon transvaalensis belongs to the warm-season grasses and is one of the economically and ecologically important crops. Cynodon species with high heterozygosity are difficult to assemble, so genome research has not been actively conducted. In this study, hybrid assembly was performed by sequencing with Illumina and PacBio. As a result of the assembly, the number of scaffolds and the length of N50 were 1,392, 928 kb, respectively. The completeness of the assembly was confirmed by BSUCO at 98.3%. In addition, as a result of estimating the size of the assembled genome by K-mer analysis (k=25), it was approximately ~413 Mb. A total of 37,060 cds sequences were annotated in the assembled genome, and their functions were identified through blast. After that, we try to complete the assembled genome into a pseudochromosome-level genome through Hi-C technology. These results will not only help to understand the complex genome composition of african bermudagrass, but also provide a resource for genomic and evolutionary studies of grass and other plant species.

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