BMB Reports
- Volume 33 Issue 3
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- Pages.208-212
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- 2000
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- 1976-670X(eISSN)
A Genetic Marker for the Korean Native Cattle (Hanwoo) Found by an Arbitrarily Primed-Polymerase Chain Reaction (AP-PCR)
- Lee, Ji-Seon (Division of Phannacy, College of Pharmacy, Yeungnam University) ;
- Lee, Chang-Hee (Division of Phannacy, College of Pharmacy, Yeungnam University) ;
- Nam, Doo-Hyun (Division of Phannacy, College of Pharmacy, Yeungnam University) ;
- Jung, Young-Ja (Division of National Medicine Evalution, Korea Food and Drug Administration) ;
- Yeo, Jung-Sou (Department of Animal Sciences, College of Natural Resources, Yeungnam University)
- Received : 1999.12.21
- Accepted : 2000.03.06
- Published : 2000.05.31
Abstract
In order to develop a specific genetic marker for the Korean native cattle (Hanwoo), an arbitrarily-primed polymerase chain reaction (AP-PCR) analysis of 6 different cattle breeds was attempted. Eight different arbitrary primers, each longer than 20-mer nucleotides, were used. In comparison to the AP-PCR patterns, several distinctive DNA bands that are specific for a certain breed were detected. When the primer Kpn-X was employed, a 280bp DNA fragment was found to be specific only for Hanwoo. In an individual analysis of Hanwoo, this AP-PCR marker was observed in 123 head of cattle among the 153 that were tested (80.4%). Nucleotide sequencing revealed that this fragment has a short microsatellite sequence of tandem repeat,