The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Genetic Diversity of Paecilomyces japonica and Cordypces militaris Strains by URP-PCR Fingerprinting

URP-PCR핵산지문에 의한 눈꽃동충하초 (Paecilomyces japonica.)와 번데기동충하초(Cordypces militaris) 유전적 다양성분석

  • Kim, Jong-Kun (JK BioTech Co Ltd.) ;
  • Kang, Hee-Wan (Graduate School of Biotechnology and Information Technology, Hankyong National University)
  • Received : 2011.11.16
  • Accepted : 2011.11.21
  • Published : 2011.12.01
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Abstract

This study was carried out to identify the genetic characteristics among isolates of Paecilomyces spp.and Cordyceps spp. by URP-PCR analysis. Twenty URP (universal rice primer) primers of 20 mer which were designed from repetitive sequence of rice, were used for producing PCR DNA fingerprints of the mushrooms. Of them, 5 URP primers, URP2F, URP2R, URP9F, URP4R, and URP17R amplified genomic DNA of the mushrooms with polymorphic PCR patterns. On isolates of Cordyceps militaris, primers URP1F, URP2R, URP6R and URP17R produced PCR polymorphic bands of 4 types. Isolates of Cordypces sp. that are isolated from different area of Korea were identical to isolate of C. militaris, while other species of Cordypces were different to the PCR profiles. However, the URP primers did not identify the polymorphism of PCR profile on isolates of P. japonica.

본 연구는 URP-PCR 핵산지문법을 이용한 Paecilomyces spp.와 Cordyceps spp.의 종간, 종내 유전적 다양성분석을 실시 하였다. 12종류의 20mer의 URP primer가적용된 바 URP2F, URP2R, URP9F, URP4R, URP17R는 종간에 특이적인 PCR다형성밴드를 형성하였으며 Cordypces militaris 균주간에는 4가지 type의 PCR 다형성이 관찰되었다. 그러나 Paecilomyces japonica의 균주내에는 동일한 PCR 다형성을 나타내었다. URP-PCR profile을 이용하여 경동시장에서 수집한 미 동정 동충하초를 동정 할 수 있었다.

Keywords

References

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