Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Random Amplified Polymorphic DNA (RAPD) Variation in Porhyra yezoensis and P. tenera

  • Beom-Kyu Kim (Department of Biological Engineering Yosu National Fisheries University) ;
  • Gyu-Hwa Chung (Department of Biological Engineering Yosu National Fisheries University) ;
  • Yuji Fujita (Algal Cultivation Laboratory, Faculty of Fisheries, Nagasaki University)
  • Published : 1997.08.01
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Abstract

The random amplified polymorphic DNA (RAPD) technique was used to anlayze six isolates of two species of Porphyra, P. yezoensis and P. tenera. Four 21-mer prrmers were combined randomly into six groups of double primers and screened for DNA amplification using nuclear and chloroplast tempate DNA. The RAPD patterns resulting from RnRc and CnCc primers provided evidence for both genetically homo-and heterogeneous populations of P. yezoensis and P. tenera. Similarity values obtained by RnRc primer analysis of nuclear DNA varied from 0.364 to 0.714 and those of chloroplast DNA were high, ranging from 0.727 to 1.000, except for P. yezoensis (Enoura).

Keywords

References

  1. Nippon Suisan Gakkaishi v.58 no.3 Comparative restriction endonuclease analysis of rhodoplast DNA from different species of Porphyra Araki, S.;T. Sakurai;T. Oohusa;N. Sato
  2. J. Phycol. v.26 Nucleotide sequence of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from Cryptomonas ${\varphi}$ : Evidence supporting the polyphletic origin of plastids Douglas, S. E.;D. G. Durnford;C. W. Morden
  3. J. Appl. Phycol. v.6 Random amplified polymorphic DNA (RAPD) identification of genetic variation in three species of Porphyra (Bangiales, Rhodophyta) Dutcher, J. A.;D. Kapraun
  4. Nippon Suisan Gakkaishi v.54 Electrophoretic variants characteristic of heterozygotes in haploid laver Porphyra sp. Fujio, Y.;P.L.G. Kodaka;M. Hara;K. Akiyama
  5. Hydrobiologia v.204/205 Protoplast isolation and fusion in Porphyra (Bangiales, Rhodophyra) Fujita, Y.;M. Saito
  6. J. Phycol. v.24 The use of plastid DNA restriction endonuclease patterns in delineating red algal species and populations Goff, L. J.;A. W. Coleman
  7. Bot. Mag. Tokyo v.105 Phylogenetic relationships in Gnetophyta deduced from rbc L gene sequences Hasebe, M.;M. Ito;R. Kofuji;K. Iwatsuki;K. Ueda
  8. J. Tokyo. Univ. Fish. v.79 Interspecific cross experiments between Porphyra yezoensis Ueda and P. tenera Kjellman (Bangiales, Rhodophyta) by using pigmentation variants Miura, A.;P. F. Fu;J. A. Shin
  9. Aquaculture v.132 Culture and development of electrically fused protoplasts from red marine algae, Porphyra yezoensis and P. suborbiculata Mizukami, Y.;M. Okauchi;H. Kito;S. Ishimoto;T. Ishida;M. Fuseya
  10. Proc. Natl. Acad. Sci. USA v.74 Mathematical model for study genetic variation in terms of restriction endonucleases Nei, M.;W.H. Li
  11. Plant Mol. Biol. v.15 Localization of β-phycoerythrin to the thylakoid lumen of Cryptomonas ${\varphi}$ does not involve a signal peptide Reith, M.;S. Douglas
  12. Plant Cell v.5 A highresolution gene map of the chloroplast genome of the red alga Porphyra purpurea Reith, M.;J. Munholland
  13. J. Phycol. v.27 Fraction of nuclear, chloroplast, and mitochondrial DNA from Polysiphonia boldii (Rhodophyta) using a rapid and simple method for the simultaneous isolation of RNA and DNA Roell, M. K.;D. E. Morse
  14. A Laboratory manual(2nd ed.) Molecular clonin Sambrook, J.;E. F. Fritsch;T. Maniatis
  15. Curr. Genet. v.19 Organization of the chloroplast genome in the red alga Porphyra yezoensis Shivji, M. S.
  16. Mar. Ecol. Prog. Ser. v.84 Rapid isolation of high molecular weight DNA from marine macroalgae Shivji, M. S.;S. O. Rogers;M. J. Stanhope
  17. Plant genetic enginering manual Uchimiya, H.;K. Tanaka;M. Sugiura
  18. Curr. Genet. v.16 The genes of both subunits of ribulose-1,5-bis-phosphate carboxylase constitute an operon on the plastome of a red algae Valentin, A. K.;K. Zetsche
  19. Nucleic Acids Res. v.18 Fingerprinting genomes using PCR with arbitrary primers Welsh, J.;M. McClelland