• Title/Summary/Keyword: Porphyra isolates

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A Study on the Relationships Between the Epiphytic Microbes and the Blight of Porphyra Species from the Coastal Waters of the Yellow Sea, Korea -III. Seasonal Variation of Heterotrophic Bacteria on the Blades of Porphyra Species Near Mokp'o- (서해산 김 엽체상의 미소생물과 김의 병해와의 관계에 대한 연구 -III. 김 엽체상에 부착된 종속영양세균의 계절적 변화-)

  • KIM Jeong-Hee;LEE Geon-Hyoung;LEE Won-Ho;KIM Joong-Rae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.25 no.4
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    • pp.314-321
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    • 1992
  • To study the seasonal variation of heterotrophic bacteria on the blades of Porphyra species, samples were collected In the intertidal zone near Mokp'o of the Yellow Sea from February to December, 1990 Annual distribution of heterotrophic bacteria on the blades of Porphyra species ranged from $6.9\times10^2\;to\;1.04\times10^5\;cfu/cm^2$ at site 1, and from $9.4\times10^3\;to\;2.93\times10^5\;cfu/cm^2$ at site 2. Average sixty-five percent of isolates from the blades of Porphyra species were rods and $52.1-82.7\%$ of isolates were Gram-negative. Most isolates showed starch hydrolysis$(68\%)$ on March, but showed Poor utilization of carbon sources and no $H_2S$ production. Dominant genus isolated from the baldes of Porphyra species were Alcaligenes spp., Moraxella spp., on March and Micrococcus spp., Moraxella spp., Corynebacterium spp., on November.

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Random Amplified Polymorphic DNA (RAPD) Variation in Porhyra yezoensis and P. tenera

  • Beom-Kyu Kim;Gyu-Hwa Chung;Yuji Fujita
    • Journal of Aquaculture
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    • v.10 no.3
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    • pp.321-326
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    • 1997
  • The random amplified polymorphic DNA (RAPD) technique was used to anlayze six isolates of two species of Porphyra, P. yezoensis and P. tenera. Four 21-mer prrmers were combined randomly into six groups of double primers and screened for DNA amplification using nuclear and chloroplast tempate DNA. The RAPD patterns resulting from RnRc and CnCc primers provided evidence for both genetically homo-and heterogeneous populations of P. yezoensis and P. tenera. Similarity values obtained by RnRc primer analysis of nuclear DNA varied from 0.364 to 0.714 and those of chloroplast DNA were high, ranging from 0.727 to 1.000, except for P. yezoensis (Enoura).

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A Study on the Relationships Between the Epiphytic Microbes and the Blight of Porphyra Species from the Coastal Waters of the Yellow Sea, Korea -II. Seasonal Variation of Heterotrophic Bacteria in the Surrounding Seawater of Porphyra Farming Area Near Mokp'o- (서해산 김 엽체상의 미소생물과 김의 병해와의 관계에 대한 연구 -II. 목포 인근 김 양식장 주변해수에서의 종속영양세균의 계절적 변화-)

  • KIM Jeong-Hee;LEE Geon-Hyoung;SHIN Yoon-Keun;KIM Joong-Rae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.25 no.4
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    • pp.307-313
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    • 1992
  • To study the seasonal variation of heterotrophic bacteria near the surrounding seawater of Porphyra forming area, samples were collected in the intertidal waters of Mokp'o of the Yellow Sea from February to December, 1990. Annual distribution of heterotrophic bacteria ranged from $7.5\times10^2\;to\;1.1\times10^5\;cfu/ml,$ annual distribution of physiological characteristic bacteria ranged from $5.0\times10\;to\;4.34\times10^4\;cfu/ml$ for proteolytic bacteria, from 0 to $1.35\times10^4\;cfu/ml$ for lipolytic bacteria and from 0 to $1.2\times10^4\;cfu/ml$ for amylolytic bacteria. Sixty-five percent of isolates from seawater were rods, and $76.7\%$ of isolates were Gram-negative. Most isolates were mesophiles and showed utilization of various carbon sources such as glucose, maltose, lactose, sucrose and arabinose. Most isolates also showed tolerance to a broad range of salt concentration. Dominant genus in seawater were Flavobacterium spp., in February, Moraxella spp., Acinetobacter spp. in March, Bacillus spp., Chromobacterium spp., Micrococcus spp., Vibrio spp. in July and Chromobacterium spp., Micrococcus spp., Bacillus spp. in November.

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Biology of Porphyra pulchella sp. nov. from Australia and New Zealand

  • Ackland, Jillian C.;West, John A.;Scott, Joseph;Zuccarello, Giuseppe C.;Broom, Judy
    • ALGAE
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    • v.21 no.2
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    • pp.193-208
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    • 2006
  • Porphyra pulchella sp. nov. Ackland, West, Scott and Zuccarello was obtained at Mimosa Rock National Park, New South Wales; Westgate Bridge, Victoria, Australia; and Waihau Bay, North Island, New Zealand. It occurs mainly in mangrove habitats and is very small (± 1 mm) in field collections. In laboratory culture at 21 ± 2°C tiny blades (0.5-3.0 mm) reproduced exclusively by archeospores liberated from vegetative cells of the upper sector of the blades. The archeospores displayed amoeboid and gliding motility once discharged. At 14 ± 2°C the blades grew to 25 mm and produced longitudinal spermatangial streaks mixed with ‘phyllosporangial’ streaks. The discharged ‘phyllospores’ showed amoeboid motility and germinated forming asexual blades. A conchocelis phase with typical bangiophycidean pit connections was observed in blade cultures after 8-10 weeks at 14 ± 2°C. Conchocelis filaments produced conchosporangia and these released amoeboid conchospores that developed into archeosporangiate blades. Molecular data indicate that all 3 isolates are genetically identical.