• Title/Summary/Keyword: japonica L.

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A taxonomic study of Korean Artemisia L. using somatic chromosome numbers (한국산 쑥속의 체세포 염색체수에 의한 분류학적 연구)

  • Park, Myung Soon;Jang, Jin;Chung, Gyu Young
    • Korean Journal of Plant Taxonomy
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    • v.39 no.4
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    • pp.247-253
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    • 2009
  • Somatic chromosome numbers for 20 taxa of Korean Artemisia L. were investigated for the purpose of classification. Somatic chromosome numbers of treated taxa were 2n = 16, 18, 34, 36, 50, 52, 54, and therefore their basic chromosome numbers were x = 8, 9, 10, 13, 17. The chromosome number of A. japonica var. angustissima is being reported for the first time in this study. The chromosome numbers of 13 taxa were the same as in previous reports; A. capillaris (2n = 18), A. japonica var. hallaisanensis (2n = 36), A. japonica subsp. littoricola (2n = 36), A. annua (2n = 18), A. carvifolia (2n = 18), A. fukudo (2n = 16), A. keiskeana (2n = 18), A. stolonifera (2n = 36), A. sylvatica(2n = 16), A. selengensis (2n = 36), A. montana (2n = 52), A. lancea (2n = 16), A. sieversiana (2n = 18); however, the chromosome numbers of 6 taxa were different; A. japonica var. japonica (2n = 18, 36 vs 2n = 36), A. sacrorum (2n = 18, 54 vs 2n = 54), A. rubripes (2n = 16, 34 vs 2n = 16), A. indica (2n = 34, 36 vs 2n = 34), A. codonocephala (2n = 18, 50, 54 vs 2n = 50), A. argyi (2n = 34, 36, 50 vs 2n =34). The somatic chromosome numbers of Korean Artemisia are thought to be good characteristics for classifying some taxa such as A. japonica var. japonica, A. sacrorum, A. codonocephala, A. argyi, A. montana, A. sylvatica.

Studies on the Duration and Rate of Grain Filling in Rice (Oryza sativa L.) I. Varietal Difference and Effects of Nitrogen (수도의 등숙기간 및 등숙속도 연구 I. 품종간 차이 및 질소의 영향)

  • 조동삼;정승근;박연규;손석용
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.1
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    • pp.103-111
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    • 1987
  • Understanding grain filling characteristics represented by grain filling duration and grain filling rate is import-ant in improving higher yielding varieties and developing better cultural methods of rice (Oryza sativa L.). Recently developed 6 Japonica and 6 Japonica/Indica varieties were grown under 3 nitrogen levels at Experimental Farm of Chungbuk National University in 1986. The range of grain filling duration of 12 varieties was 20.9-39.0 days, while grain filling rate ranged from 53.8 to 136.6 mg. panicle$^{-1}$. day$^{-1}$. Although the difference of the average grain filling duration between Japonica varieties and Japonica/Indica was less than 4 days, the average grain filling rate of Japonica/lndica varieties was greater than Japonica more than 30%. Samgangbyeo showed the shortest grain filling duration of 21.0-24.2 days and the greatest grain filling rate of 119.3-143.8 mgㆍpanicle$^{-1}$. day$^{-1}$ under 3 nitrogen levels, while Seomjinbyeo and Milyang 23 showed the quite opposite grain filling characteristics. Nitrogen levels did not show any significant effects on grain filling characteristics. Negative correlation was round between grain filling duration and grain filling rate, and significant positive correlations of grain filling rate with grains/panicle, grain weight and panicle weight indicated that grain filling rate is more important characteristics of grain filling. Pathway analysis revealed that contribution of grain filling rate to panicle weight is rather indirect through grain weight.

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Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites

  • Park, Hana;Yoon, Chang Young;Kim, Jin Sook;Kim, Joo-Hwan
    • Korean Journal of Plant Resources
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    • v.28 no.6
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    • pp.743-751
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    • 2015
  • Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica- specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.

Quantitative Analysis of Flavonoid Glycosides in Sophora japonica and Sophora flavescens by HPLC-DAD

  • Kim, Soo Sung;Park, SeonJu;Kim, Nanyoung;Kim, Seung Hyun
    • Natural Product Sciences
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    • v.27 no.4
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    • pp.284-292
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    • 2021
  • Recently, a phytoestrogenic functional food has been developed using the fruits of Sophora japonica. Phytochemical investigation of fruits of S. japonica led to the isolation of eight flavonoid glycosides using various chromatographic techniques. The isolated compounds were identified as genistin (1), sophoricoside (2), genistein 7,4'-di-O-β-D-glucopyransoide (3), sophorabioside (4), genistein-7-O-β-D-glucopyranoside-4'-O-[(α-L-rhamnopyranosyl)-(1→2)-β-D-glucopyranoside] (5), sophoraflavonoloside (6), nicotiflorin (7) and kaempferol-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranosyl-(1→3)-β-D-glucopyranoside (8), respectively, by comparison of their spectroscopic data with those reported in the literature. In addition, a new HPLC-DAD method for simultaneous determination of the isolated compounds was developed to quantitate the contents of flavonoids in S. japonica and S. flavescens. The method was validated in terms of limit of detection, limit of quantitation, specificity, linearity, precision and accuracy. The validated method was successfully applied to determine eight flavonoids in two Sophora species. The contents of eight flavonoids varied according to the parts and species. Particularly, it was found that only the fruits of S. japonica contained sophoricoside, a phytoestrogenic isoflavone.

Quality Characteristics of Maejakgwa Containing Various Levels of Eriobotrya japonica Leaf Powder (비파 분말을 첨가한 매작과의 품질 특성)

  • Cho, Hee-Sook;Kim, Kyung-Hee
    • Journal of the East Asian Society of Dietary Life
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    • v.22 no.4
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    • pp.550-557
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    • 2012
  • The purpose of this study was to investigate the quality characteristics of Maejakgwa prepared with Eriobotrya japonica leaf powder (0, 1, 3, 5, 7%) substituted for flour. The pH and density of Maejakgwa dough significantly decreased in response to addition of all levels of E. japonica leaf powder. Furthermore, when spread factor values were compared among the groups, they were found to be inversely proportional to the E. japonica leaf powder concentration. With increasing amounts of E. japonica leaf powder, L and a values decreased, whereas b value increased. In terms of textural characteristics, addition of E. japonica leaf powder increased hardness, cohesiveness, springiness, chewiness, and brittleness compared to control. Finally, the results of an acceptance test showed that Maejakgwa containing 5% E. japonica leaf powder had the highest scores.

Lichen Flora of Ullung Island(I) -Graphis and Pyrenula Genera- (울릉도의 지의류 (I) -Graphis와 Pyrenula속 종의 분류-)

  • Ka, Kang-Hyeon;Park, Hyun;Ryoo, Cheon-In
    • The Korean Journal of Mycology
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    • v.25 no.2 s.81
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    • pp.77-84
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    • 1997
  • The samples of 26 lichens were collected during two field trips to Ullung Island in May and October, 1995. From the samples collected, three species in two genera were identified; Graphis rikuziensis (Vain.) Nak., G. scripta (L.) Ach. and Pyrenula japonica Kurok. Among them, P. japonica was identified for the first time in Korea and is described here in detail.

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Effects of Hot Aqueous and Ethanol Extract from $Lonicera$ $japonica$ $Flos$ on NO and $PGE_2$ in Macrophage (금은화 추출액이 RAW 264.7 Macrophage에서의 NO와 $PGE_2$ 생성에 미치는 영향)

  • Yun, Kyung-Jin;Lee, Eun-Yong
    • Journal of Acupuncture Research
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    • v.29 no.1
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    • pp.67-74
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    • 2012
  • Objectives : The objective of this study is to study the effects of hot aqueous extract and ethanol extract from $Lonicera$ $japonica$ $Flos$ on nitric oxide(NO) and prostaglandin $E_2(PGE_2)$ production in macrophage. Methods : $Lonicera$ $japonica$ $Flos$ was extracted in two ways. One was extracted with distilled water(2L) for 4 h and the other one was extracted with 70% ethanol (2L) for 4h. The RAW 264.7 macrophage was subclutured. In order to evaluate cytotoxicity, MTT assay was performed. The concentrations of NO were measured by Griess assay. The concentrations of $PGE_2$ were measured by enzyme immunoassay. Results : 25, $125{\mu}g/m{\ell}$ hot aqueous extract from $Lonicera$ $japonica$ $Flos$ inhibited NO production in LPS-stimulated RAW 264.7 macrophages significantly. 25, 125, $625{\mu}g/m{\ell}$ ethanol extract from $Lonicera$ $japonica$ $Flos$ inhibited NO production in LPS-stimulated RAW 264.7 macrophages significantly. 150, $200{\mu}g/m{\ell}$ hot aqueous extract and ethanol extract from $Lonicera$ $japonica$ $Flos$ inhibited $PGE_2$production in LPS-stimulated RAW 264.7 macrophages significantly. Conclusions : This study suggests that hot aqueous extract and ethanol extract from $Lonicera$ $japonica$ $Flos$ suppress NO and $PGE_2$ production. So hot aqueous extract and ethanol extract from $Lonicera$ $japonica$ $Flos$ may have an anti-inflammation effect.

Anti-Obesity and Lipid Lowering Effect of Discorea japonica Thunb. Fermented with Monascus in High-Fat Diet Induced Obese C57BL/6J Mice Model (고지방식이로 유도된 C57BL/6J 마우스 비만모델에서 참마홍국발효의 비만 억제 및 지질 저하 효과)

  • Oh, Deuk Chang;Kang, Soon Ah
    • The Korean Journal of Food And Nutrition
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    • v.34 no.5
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    • pp.526-536
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    • 2021
  • This study was conducted to investigate the anti-obesity effect of Discorea Japonica Thunb. fermented with Monascus After inducing obesity by feeding hight fat diet (diet induced obesity model: DIO) for four weeks, each 8 rats were assigned to normal (Nor), high fat diet (HF), high fat diet containing orlistat (PC), high fat diet containing different concentration of Discorea Japonica Thunb. fermented with Monascus (UPDM_L, UPDM_H) and Discorea Japonica Thunb. (UPD) extract. Although the UPD, UPDM_L (ultrafine pulverized Discorea Japonica Thunb. fermented with Monascus: 400 mg/kg) and UPDM_H (DIO oral administration ultrafine pulverized Discorea Japonica Thunb. fermented with Monascus: 800 mg/kg) showed weight gain inhibition effects, the results of poor obesity inhibition rather than PC were confirmed. However, it showed a more effective weight loss effect in UPDM_H than UPD, and significantly reduced the weight of epididymal fat and subcutaneous fat. Furthermore, the possibility of anti-obesity effects of Discorea Japonica Thunb. fermented with Monascus can be confirmed by observing the effects of reducing serum total cholesterol, triglyceride and LDL concentrations, reducing ALT and AST levels, and inhibiting fat build-up in liver tissue. It is believed that Discorea Japonica Thunb. fermented with Monascus can be expected to utilize as a functional material that is important to improve anti-obesity and metabolic syndrome.

Study of Mantispa japonica Maclachlan (Neuroptera : Mantispidae), Parasitoid of Nephila clavata L. Koch (무당거미 (Nephila clavata)의 알집에 기생하는 애사마귀붙이 (Mantispa japonica)에 관한 연구)

  • 김주필;이영보;장승종;김미애
    • The Korean Journal of Soil Zoology
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    • v.2 no.1
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    • pp.10-15
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    • 1997
  • Out of Nephila clavata cocoon collected from pine tree in Hwasung-Gun, Kyungi-Do in 1996 and 1997 Adults of Mantispa japonica were emerged and found to feed on both eggs and newly hatching Nephila clavata nymphs.

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The Occurrence of Griffithsia okiensis (Ceramiaceae, Rhodophyta) from Korea on the Basis of Morphology and Molecular Data

  • Kim, Hyung-Seop;Yang, Eun Chan;Boo, Sung Min
    • ALGAE
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    • v.21 no.1
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    • pp.91-101
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    • 2006
  • Despite continued studies on red algal flora in Korea, the taxonomy of the tiny ceramiaceous algae has received little attention. We report for the first time Griffithsia okiensis from Korea on the basis of morphology and molecular data. The species is small in thalli height (0.3-1.5 cm), and in diameter of vegetative cells (50-500 μm), and the ratio of cell length/breadth is 2-3 times. It has two carpogonial branches from the supporting cell of procarp. We generated psbA and rbcL sequences from ten specimens of G. okiensis isolated from Korea and Japan and from one G. japonica species isolated Japan. Eight specimens of G. okiensis from Korea were almost identical in both psbA and rbcL regions, nevertheless they differed from Japanese specimens by 4 ucleotides in psbA and 7 in rbcL. In all analyses of psbA, rbcL, and psbA + rbcL data sets, G. okiensis was determined to be a different species from G. japonica isolated from Japan, although both species showed a sister relationship. For all that extensive collection trips, we found no evidence for the occurrence of G. japonica in Korea.