• The Korean Journal of Physiology and Pharmacology
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• v.20 no.1
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• pp.63-67
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• 2016

Severe graft-versus-host disease (GVHD) is an often lethal complication of allogeneic hematopoietic stem cell transplantation (HSCT). The safety of clinical-grade mesenchymal stem cells (MSCs) has been validated, but mixed results have been obtained due to heterogeneity of the MSCs. In this phase I study, the safety of bone marrow-derived homogeneous clonal MSCs (cMSCs) isolated by a new subfractionation culturing method was evaluated. cMSCs were produced in a GMP facility and intravenously administered to patients who had refractory GVHD to standard treatment resulting after allogeneic HSCT for hematologic malignancies. After administration of a single dose ($1{\times}10^6cells/kg$), 11 patients were evaluated for cMSC treatment safety and efficacy. During the trial, nine patients had 85 total adverse events and the rate of serious adverse events was 27.3% (3/11 patients). The only one adverse drug reaction related to cMSC administration was grade 2 myalgia in one patient. Treatment response was observed in four patients: one with acute GVHD (partial response) and three with chronic GVHD. The other chronic patients maintained stable disease during the observation period. This study demonstrates single cMSC infusion to have an acceptable safety profile and promising efficacy, suggesting that we can proceed with the next stage of the clinical trial.

• Proceedings of the KIEE Conference
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• 2003.07b
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• pp.909-911
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• 2003

The almost pole transformers are constructed with tank, cover, clamp etc., that contains the insulation oil, core, coil, terminals, bus and the other accessories. If some fault current will be flown by some trouble or accident, interior pressure of the transformer shall be very quickly rise, and mechanical components or insulation oil from the transformer enclosure shall be propelled or dropped from the tank. For the prevention of the above accident, recently the pole transformers should be done 'the Design Tests for Fault Current Capability' according to ANSI C57.12.20(1997) There are two tests method in this standard, Test Number I with a high current arcing fault, without internal fusible elements, shall be conducted on rack enclosure with its minimum designed air space. Test Number II with an internal fusible element, shall be conducted on each enclosure diameter utilizing the internal fusible elements. KEPCO recently request to be done the 'Design Tests for Fault Current Capability' for pole transformers according to KEPCO's standard ES141-$533{\sim}545$, PS141-$482{\sim}518$ and RS141-$611{\sim}628$ that is same with Test Number I of ANSI C57.12.20.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.19 no.3
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• pp.240-249
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• 2009

HRSG(Heat Recovery Steam Generator) building is large enclosed structure included various heat sources. This building needs to appropriately keep internal air temperature for worker's safety and operability of control devices. In this study, ventilation analysis is performed to find proper ventilation method for temperature control. Ventilation analysis is applied to entire internal space of the building with standard $k-{\varepsilon}$ model and enhanced wall treatment because of large size of the structure. And the ventilation method is considered natural and forced convection with two louver structures which has damper or not. Louver structure affect directly air circulation in near HRSG and lower region of the building. Forced ventilation provides strong inertial force which cause upward airflow. From the analysis, it is found that design requirement for internal air temperature can be satisfied by forced ventilation method with louver structure without damper.

• Archives of Pharmacal Research
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• v.27 no.12
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• pp.1202-1206
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• 2004

A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/ MS) method for the determination of lithospermic acid B (LSB) in rat serum was developed. LSB and internal standard, 7-hydroxy-3-phenyl-chromen-4-one (HPC) were extracted from rat serum with methyl-tert-butyl ether at acidic pH and analyzed on a Luna $C_8$ column with the mobile phase of acetonitrile-ammonium formate (10 mM, pH 6.5) (50:50, v/v). The analytes were detected using a negative electrospray ionization tandem mass spectrometry in the multiple- reaction-monitoring mode. The standard curve was linear $(r^2 = 0.997)$ over the concentration range of 10.0-500 ng/mL. The coefficient of variation and relative error for intra- and interassay at three QC levels were 1.1~6.2% and -10.3~-2.7%, respectively. The recovery of LSB from serum sample ranged from 73.2 to 79.5%, with that of HPC (internal standard) being 75.1 %. The lower limit of quantification for LSB was 10 ng/mL using 50 ${\mu}L$ of serum sample.

• Analytical Science and Technology
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• v.15 no.4
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• pp.335-340
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• 2002

An analytical method has been developed to determine multi-elements in human hair samples by inductively coupled plasma mass spectrometry (ICP-MS). 0.05 g of hair sample was added to the Teflon digestion bomb together with 1.5 mL of nitric acid and an appropriate amount of In as an internal standard. The sample was then decomposed in the microwave digestion system. The hair certified reference material, GBW 09101, was analyzed for the validation of the analytical method. The determined values were in good agreement with the certified values within the uncertainty range.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.05a
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• pp.507-509
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• 2022

In order to collect vehicle internal diagnostic data, it is necessary to collect diagnostic data of ECU (Electronic Control Unit) included in various automotive parts. Diagnostic communication can be used to collect diagnostic data of ECU. In this paper, the method of collecting diagnostic data according to ECU function through standard diagnostic communication and the diagnostic communication module were analyzed. Among the standard modules of AUTOSAR, a standard for automotive electronics used by many automobile manufacturers, the diagnostic communication module was studied, and the process of diagnostic data processing through ECU was studied.

• The Journal of Advanced Prosthodontics
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• v.9 no.4
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• pp.265-270
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• 2017

PURPOSE. This study is to evaluate the internal fit of the crown manufactured by CAD/CAM milling method and 3D printing method. MATERIALS AND METHODS. The master model was fabricated with stainless steel by using CNC machine and the work model was created from the vinyl-polysiloxane impression. After scanning the working model, the design software is used to design the crown. The saved STL file is used on the CAD/CAM milling method and two types of 3D printing method to produce 10 interim crowns per group. Internal discrepancy measurement uses the silicon replica method and the measured data are analyzed with One-way ANOVA to verify the statistic significance. RESULTS. The discrepancy means (standard deviation) of the 3 groups are $171.6\;(97.4){\mu}m$ for the crown manufactured by the milling system and 149.1 (65.9) and $91.1\;(36.4){\mu}m$, respectively, for the crowns manufactured with the two types of 3D printing system. There was a statistically significant difference and the 3D printing system group showed more outstanding value than the milling system group. CONCLUSION. The marginal and internal fit of the interim restoration has more outstanding 3D printing method than the CAD/CAM milling method. Therefore, the 3D printing method is considered as applicable for not only the interim restoration production, but also in the dental prosthesis production with a higher level of completion.

• Archives of Pharmacal Research
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• v.29 no.4
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• pp.339-342
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• 2006

A simple high performance liquid chromatographic (HPLC) method was developed for the determination of tolperisone in human plasma. Tolperisone and internal standard (chlorphenesin) were isolated from 1 mL of plasma using 8 mL of dichlormethane. The organic phase was collected and evaporated under nitrogen gas. The residue was then reconstituted with 300 mL aliquot of mobile phase and a 100 mL aliquot was injected onto the $C_{18}$ reverse-phased column. The mobile phase, $45\%$ methanol containing $1\%$ glacial acetic acid and $0.05\%$ 1-hexanesulfonic acid was run at a flow rate of 1 mL/min. The column effluent was monitored using UV detector at 260 nm. The retention times for tolperisone and the internal standard were approximately 7.1 and 8.4 min, respectively. The standard curve was linear with minimal intra-day and inter-day variability. The quantification limit of tolperisone in human plasma was 10 ng/ mL. The proposed method has been applied to the determination of pharmacokinetic profile of tolperisone in Koreans. The T max of tolperisone in Koreans $(0.94{\pm}0.42\;h)$ was not significantly differ from that reported in Europeans (0.5-1 h), but the mean half-life in Koreans $(1.14{\pm}0.27\;h)$ was shorter than that in Europeans $(2.56{\pm}0.2\;h)$. The proposed HPLC method is simple, accurate, reproducible and suitable for pharmacokinetic study of tolperisone.

• Bulletin of the Korean Chemical Society
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• v.33 no.5
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• pp.1693-1698
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• 2012

A headspace gas chromatographic mass spectrometric (GC-MS) assay method was developed for the simultaneous determination of methyl tertiary butyl ether (MTBE), $tert$-butyl alcohol (TBA) and benzene, toluene, ethyl benzene and xylene (BTEX) in soil contaminated with gasoline. 2 g of soil sample were placed in a 10 mL headspace vial filled with 5 mL of phosphoric acid solution (pH 3) saturated with NaCl, and the solution was spiked with fluorobenzene as an internal standard and sealed with a cap. The vial was heated in a heating block for 40 min at $80^{\circ}C$. The detection limits of the assay were 0.08-0.12 ${\mu}g$/kg for the analytes. For five independent determinations at 10 and 50 ${\mu}g$/kg, the relative standard deviations were less than 10%. The method was used to analyze fifty six soil samples collected from various regions contaminated with gasoline in Korea. The developed method may be valuable for the monitoring of the analytes in soil.

• Proceedings of the Korea Concrete Institute Conference
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• 2000.04a
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• pp.159-162
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• 2000

The purpose of this study is to develope an accurate procedure for measuring the glass content of fly ash produced by bituminous coal to assist in predicting their behavior in concrete. Quantitative mineralogical compositions of fly ashes were obtained by internal standard method using powder X-ray diffraction analysis. In the X-ray diffraction method, the specimen and standard fluorite were cautiously mixed and pulverized to 22$\mu$m in wet process so as to avoid the prefered orientation of the crystal and microabsorption effect of X-ray. calibration curve were fitted for several references peaks of four phases:$\alpha$-quartz. mullite, magnetite, hematite. The amount of glass was calculated by subtracting the amount of crystal phase and loss on ignition from the total amount. Glass content determined with this method ranged from 66.7 to 75.wt%.