• 한국식품영양학회지
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• 제29권1호
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• pp.1-11
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• 2016

To enhance the physiological activities of roasted coffee (RC), 30 kinds of green coffee beans (GCB) with different cultivating areas and varieties were fermented with Monascus ruber mycelium (MR) by solid-state culture. After the dried MR-fermented GCB was subjected medium roasting, each RC was extracted with hot-water. Among the hot-water extracts, the highest yield was the hot-water extract of RC from MR-fermented Indonesia Mandheling GCB (15.5%). However, the hot-water extract of RC from MR-fermented Ethiopia Sidamo GCB showed significantly higher polyphenolic contents (3.08 mg GAE/100 mg) and ABTS free radical scavenging activity (25.41 mg AEAC/100 mg). Meanwhile, the hot-water extract of RC from MR-fermented Vietnam Robusta GCB showed not only the effective inhibition of $TNF-{\alpha}$ level (73.7% inhibition of LPS-stimulated control) from LPS-stimulated RAW 264.7 cells but also significant inhibition of lipogenesis (63.5% inhibition of lipid differentiation control) in 3T3-L1 pre-adipose cells. In conclusion, these results suggest that roasted coffees from Ethiopia Sidamo and Vietnam Robusta green coffee beans fermented with Monascus ruber mycelium using solid-state culture could have industrial applications as functional coffee beverages.

• Journal of Microbiology and Biotechnology
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• 제28권6호
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• pp.883-892
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• 2018

Probiotics, including Enterococcus faecium, confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faecium by 16S rDNA gene sequence analysis, and designated as E. faecium L11. To evaluate the potential of E. faecium L11 as a probiotic, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E. faecium L11 showed >66% and >62% survival in artificial gastric juice (0.3% pepsin, pH 2.5) and simulated small intestinal juice (0.5% bile salt and 0.1% pancreatin), respectively. Heat-killed E. faecium L11 significantly (p < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines (IL-6 and $TNF-{\alpha}$) by activated macrophages obtained from ICR mice. In addition, E. faecium L11 showed a protective effect against Salmonella Typhimurium infection in Caenorhabditis elegans. In addition, feeding E. faecium L11 significantly (p < 0.05) extended the lifespan of C. elegans compared with the control. Furthermore, genes related to aging and host defense were upregulated in E. faecium L11-fed worms. In conclusion, E. faecium L11, which prolongs the lifespan of C. elegans, may be a potent probiotic supplement for livestock.

• Journal of Ginseng Research
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• 제44권1호
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• pp.145-153
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• 2020

Background: Panax ginseng Meyer (Araliaceae) is a highly valued medicinal plant in Asian regions, especially in Korea, China, and Japan. Chemical and biological studies on P. ginseng have focused primarily on its roots, whereas the seeds remain poorly understood. This study explores the phytochemical and biological properties of compounds from P. ginseng seeds. Methods: P. ginseng seeds were extracted with methanol, and 16 compounds were isolated using various chromatographic methods. The chemical structures of the isolates were determined by spectroscopic data. Antiinflammatory activities were evaluated for triterpene and steroidal saponins using lipopolysaccharide-stimulated RAW264.7 macrophages and THP-1 monocyte leukemia cells. Results: Phytochemical investigation of P. ginseng seeds led to the isolation of a novel triterpene saponin, pseudoginsenoside RT₈, along with 15 known compounds. Pseudoginsenoside RT₈ exhibited more potent antiinflammatory activity than the other saponins, attenuating lipopolysaccharide-mediated induction of proinflammatory genes such as interleukin-1β, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2, and matrix metalloproteinase-9, and suppressed reactive oxygen species and nitric oxide generation in a dose-dependent manner. Conclusion: These findings indicate that pseudoginsenoside RT₈ has a pharmaceutical potential as an antiinflammatory agent and that P. ginseng seeds are a good natural source for discovering novel bioactive molecules.

• 대한안전경영과학회지
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• 제11권4호
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• pp.221-228
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• 2009

Electronic Product Code Information Services(EPCIS) is an EPCglobal standard for sharing EPC related information between trading partners. EPCIS provides a new important capability to improve efficiency, security, and visibility in the global supply chain. EPCIS data are classified into two categories, master data (static data) and event data (dynamic data). Master data are static and constant for objects, for example, the name and code of product and the manufacturer, etc. Event data refer to things that happen dynamically with the passing of time, for example, the date of manufacture, the period and the route of circulation, the date of storage in warehouse, etc. There are four kinds of event data which are Object Event data, Aggregation Event data, Quantity Event data, and Transaction Event data. This thesis we propose an event-based data model for EPC Information Service repository in RFID based integrated logistics center. This data model can reduce the data volume and handle well all kinds of entity relationships. From the point of aspect of data quantity, we propose a formula model that can explain how many EPCIS events data are created per one business activity. Using this formula model, we can estimate the size of EPCIS events data of RFID based integrated logistics center for a one day under the assumed scenario.

• Fisheries and Aquatic Sciences
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• 제21권5호
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• pp.14.1-14.8
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• 2018

The objective of this study was to investigate the anti-inflammatory effects of the pepsinolytic hydrolysate from the fish frame, Johnius belengerii, on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The J. belengerii frame hydrolysate (JFH) significantly suppressed nitric oxide (NO) secretion on LPS-stimulated RAW264.7 macrophages. Moreover, the JFH markedly inhibited the levels of protein and mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Furthermore, the LPS-stimulated mRNA expression of pro-inflammatory cytokines, including tumor necrosis factor $(TNF)-{\alpha}$, interleukin $(IL)-1{\beta}$, and IL-6 was downregulated when cells were cultured with the JFH. The JFH significantly reduced the phosphorylation of c-Jun N-terminal kinase (JNK) and the translocation of nuclear factor-kappa B ($NF-{\kappa}B$) in macrophages. As the result, the JFH has the potential anti-inflammatory activity via blocking the JNK and $NF-{\kappa}B$ signal pathways.

• Journal of Electrochemical Science and Technology
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• 제9권3호
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• pp.169-175
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• 2018

Cu-Salen complex was prepared and attached into chitosan (Cs) polymer backbone. Nanocomposite of the synthesized polymer was prepared with functionalized carbon nano-particles (Cs-Cu-sal/C) to modify the electrode surface. The surface morphology of (Cs-Cu-sal/C) nanocomposite film showed a homogeneous distribution of carbon nanoparticles within the polymeric matrix. The cyclic voltammogram of the modified electrode exhibited a redox behavior at -0.1 V vs. Ag/AgCl (3 M KCl) in 0.1 M PB (pH 7) and showed an excellent hydrogen peroxide reduction activity. The Cs-Cu-sal/C electrode displays a linear response from $5{\times}10^{-6}$ to $5{\times}10^{-4}M$, with a correlation coefficient of 0.993 and detection limit of $0.9{\mu}M$ (at S/N = 3). The sensitivity of the electrode was found to be $0.356{\mu}A\;{\mu}M^{-1}\;cm^{-2}$.

• 산업공학
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• 제22권3호
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• pp.243-251
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• 2009

The driving force of the manufacturing field is members' mind-set and activity. However, there is no appropriate program of change management for workers who work in the manufacturing field. Most of the small group activities were focused on the fixed tools and processes to make improvement only, not the change itself. Therefore, it was difficult to have self generating energy without actually motivating people of change. Study of IFI(Integrated Field Innovation) means change of management. This study will introduces IFI which is a new methodology of change and innovation of workers, and will describe how workers participated in the activities voluntarily. The principle of IFI is that letting employees to find the problems of the sites -anything that makes employees considering as the problem while they are working- and solve those problems through any tools own their own. Workers do not have to waste their time on bounding by unnecessary tools and processes. There are several cases that company "D" has been actually applied the IFI methodology in 2008. Through these cases, IFI proved that it was a optimized methodology for workers who work in the manufacturing field.

• 한국CDE학회논문집
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• 제4권4호
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• pp.327-338
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• 1999

In this paper, we propose the framework of an integrated design system as a CAD system of a new concept that can incorporate the design information. Current CAD systems are powerful in representing the shape of a product with solid models but cannot provide the design information that is used or created in designing the product. Therefore an integrated design system is proposed to enable the storage and usage of the design information in addition to the shape manipulation. In order to represent the design information, we define element object that represents design element such as a part, a sub-assembly or an assembly by using object-oriented methodology. The element object carries various design information such as design parameters, documents, catalogues, functions, and behaviors as well as shapes. By using the stored information, the system can provide a designer with some functions to assist design activity such as interrogating necessary information, setting design parameters and goals, and constructing constraints among design elements. A mailing system is also constructed among element objects to assist a collaborative design. In addition, an evolutionary design method using genetic algorithms is realized within the proposed framework.

• Journal of Photoscience
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• 제5권2호
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• pp.73-81
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• 1998

Light signals constitute major factors in regulating gene expression and morphogenesis in plants and fungi. Phytochrome A and B were well characterized red and far-red light receptors in plants. Red light signals increased the phosphorylation of 18 kDa protein, which was identified to be nucleoside diphosphate (NDP) kinase. The NDP kinase catalyzed autophosphorylation and had a protein kinase activity similar to MAP (mitogen activated protein) kinase. As candidates for blue light photoreceptors, cDNAs for CRY1 and CRY2 were isolated. The N-teminal regions of these proteins showed a high hornology to DNA photolyase. The 120 kDa protein first detected in Pisurn sativurn, which showed blue light induced phosphorylation was also detected in Arabidopsis thaliana. The 120 kDa protein was encoded by the nphl gene, which regulated positive phototropism of the plant. In Neurospora crassa, blue light irradiation of the membrane fraction prepared from roycelia stimulated the phosphorylation of the 15 kDa protein, which was also identifmd to be an NDP kinase. Recent progress in understanding early events in light signal transduction mainly in Pisum sativum Alaska, Arabidopsis thaliana and Neurospora crassa was summarized.

• 한국과학교육학회지
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• 제14권1호
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• pp.45-57
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• 1994

The purpose of this study is to verify whether the FAST program is the Inquiry Science Curricular Materials, through the Comparison and Analysis of Inquiry Activities between the FAST program and our Secondary Science Books. The results of this study are as follows ; 1. FAST has 226 tasks of the Inquiry Activities, which is analyzed over two times than our text. 2. In level one, FAST holds the parts of Synthesizing Results and Evaluation, Hypothesizing and Designing an Experiment but u.ese aren't found in our text. 3. In level two, our text is analyzed No Discussion 72.2%, Demonstrating or Verifying the Content of the Text 82%, but FAST has Discussion Guided 81.8%, and isn't found any tesk of Demonstrating or Verifying the Content of the text. 4. In level three, our text is exposed a typical type I and analyzed Inquiry Index 15-25 ( Middle ), but FAST is found type IV, excepting Manipulating Apparatus and Observation and analyzed Inquiry Index over 35 ( Very - High ). Therefore, FAST Program is proved to be the desirable Inquiry Science Curricular Materials. In future, this worker is to arrange the results of the following paper as follows ; 1. The verification of the FAST Program by means of the Integrated Science Curricular Materials. 2. The development of the Inquiring, Integrated Science Curricular Materials through the results of the preceding study.