Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Probiotic Potential of Enterococcus faecium Isolated from Chicken Cecum with Immunomodulating Activity and Promoting Longevity in Caenorhabditis elegans

  • Sim, Insuk (Department of Integrated Biomedical and Life Sciences, College of Health Science, Korea University) ;
  • Park, Keun-Tae (Department of Research and Development Center, Milae Resources ML Co. Ltd.) ;
  • Kwon, Gayeung (Department of Public Health Science (BK21 PLUS Program), Graduate School, Korea University) ;
  • Koh, Jong-Ho (Department of Bio-Food Analysis and Processing, Bio-Campus Korea Polytechnic College) ;
  • Lim, Young-Hee (Department of Integrated Biomedical and Life Sciences, College of Health Science, Korea University)
  • Received : 2018.02.13
  • Accepted : 2018.04.05
  • Published : 2018.06.28
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Abstract

Probiotics, including Enterococcus faecium, confer a health benefit on the host. An Enterococcus strain was isolated from healthy chicken cecum, identified as E. faecium by 16S rDNA gene sequence analysis, and designated as E. faecium L11. To evaluate the potential of E. faecium L11 as a probiotic, the gastrointestinal tolerance, immunomodulatory activity, and lifespan extension properties of the strain were assayed. E. faecium L11 showed >66% and >62% survival in artificial gastric juice (0.3% pepsin, pH 2.5) and simulated small intestinal juice (0.5% bile salt and 0.1% pancreatin), respectively. Heat-killed E. faecium L11 significantly (p < 0.05) increased immune cell proliferation compared with controls, and stimulated the production of cytokines (IL-6 and $TNF-{\alpha}$) by activated macrophages obtained from ICR mice. In addition, E. faecium L11 showed a protective effect against Salmonella Typhimurium infection in Caenorhabditis elegans. In addition, feeding E. faecium L11 significantly (p < 0.05) extended the lifespan of C. elegans compared with the control. Furthermore, genes related to aging and host defense were upregulated in E. faecium L11-fed worms. In conclusion, E. faecium L11, which prolongs the lifespan of C. elegans, may be a potent probiotic supplement for livestock.

Keywords

References

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