• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.6
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• pp.984-991
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• 1997

New tumor suppressor gene, snm23, homologous to human nm23/NDP kinase (human nucleoside diphosphate kinase) gene whose product has a tumor metastasis inhibitory activity, was first cloned from Korean tiger shark (Scyliorhinus forazame) skin cDNA library constructed by using a $\lambda$ ZAP-II cDNA synthesis kit. About $1\times10^5$ plaques were screened and several positive plaques were isolated and confirmed by second screening. The phagemid containing a positive clone from the Uni-Zap XR vector was excised in vivo and the gene containing the tumor metastasis suppressor protein was named as snm23. Cloned gene, snm23, was sequenced with ABI-PRISM 310 Genetic Analyzer. The nucleotide and deduced amino acid sequences of snm23 have shown an open reading frame consisting of 450 base pairs that correspond to a protein of 150 amino acid residues, with a calculated molecular mass of 16.8 kDa. Sequence comparison of snm23 with human nm23/NDP kinase was performed by using Blast protein data base of National Center for Biotechnology Information. In order to determine tissue specificity, reverse transcription-polymerase chain reaction (RT-PCR) was used. Good expression level of snm23/NDP kinase was detected at the tissues from skin, cartilage, and liver of Korean tiger shark.

• Journal of the Korean Solar Energy Society
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• v.31 no.1
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• pp.51-58
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• 2011

The energy makes the basic element which improves the quality of life with motive power of industry and life. However, using the fossil fuel resources was restricted through it's abuse and exhaustion, and that cause a global warming resultingly. According to the reason, the world increased the interest that are stability and use of new and renewable energy which is clean energy with environment. Therefore, the property data of new and renewable is needed for developing and supplying the energy. In other words, the data of new and renewable energy becomes the standards for supply and evaluation of new and renewable energy with development of industry and technology. Also, the necessity came to the fore as the reference and standards of new and renewable energy data. Therefore, in this study, we evaluate and collect the solar radiation data as the new and renewable data and process the collected data through the standards for valuation. We evaluate uncertainty with standards which are NREL, WMO, and GUM. Whereby the data becomes reference standards data and gains the credibility. For the reliability data, we correct the measuring instrument with correction period. Using the DQMS and SERI QC, we efficiently manage and evaluate the solar radiation data. As a result, we evaluate uncertainty as 1,120 case about 16 area. we achieve credibility of data from evaluated solar radiation data and provide an accurate information to user. The annual average of horizontal radiation presents between 1,484 and 4,577, then the uncertainty evaluates from 163 to 453. The error of uncertainty presents smaller than the measurement values. So, we judge a credibility of data by expression of reliability quantitatively. In additional, the reference standards data which is possible to approach anywhere will be used for the supporting related industry and policy making.

• The Journal of the Korea Contents Association
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• v.8 no.9
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• pp.84-93
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• 2008

Since 2005, the production of feature 3D animation has been growing up worldwide and technology of hardware and software has been advanced fully enough to get over the limitation of visual expression, so that rendering weight of the whole work process is getting more important. A final file in the state right before rendering increases. the data size gets bigger. Therefore, if such final file is rendered as it is, the production schedule may not be met. For, some needless time shall be spent to call and save a high-capacity file in amendment work and to execute the rendering entirely again in addition to amendment. In this study, Maya API [1]was used to study the efficient information amendment method of extracting the rendering layers' files in order to develop a tool that executes rendering by applying such method. As the result, compliance to production schedule that is important in animation production can be done through the independent rendering tool based layer rendering that can maximize the efficiency of amendment work.

• Journal of Microbiology and Biotechnology
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• v.16 no.9
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• pp.1399-1404
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• 2006

The roots of Aralia continentalis (AC) have been used traditionally in Korean as a folk medicine for anti-inflammation and as an anti-rheumatic. In this study, we report that the ethyl acetate-soluble traction (ACE) of the methanolic extract of AC root inhibited the cell growth of various human cancer cell lines and induced apoptosis of HL-60, human promyelocytic leukemia cells. Its $IC_{50}$ values on growth inhibition were estimated to be $56.3{\mu}g/ml$ on HL-60, $87.2{\mu}g/ml$ on HepG2, $93.2{\mu}g/ml$ on HeLa, $135.5{\mu}g/ml$ on DU-145, and $135.8{\mu}g/ml$ on HT-29 cells. Interestingly, ACE showed no antiproliferative effect on normal lymphocyte cells used as control. Furthermore, nuclear DAPI staining revealed the typical nuclear features of apoptosis in the HL-60 cells exposed to $80{\mu}g/ml$ ACE, and a flow cytometric analysis of the HL-60 cells using propidium iodide showed that the apoptotic cell population increased gradually from 5% at 0 h to 16% at 12 h and 20% at 24 h after treated with $50{\mu}g/ml$ of ACE. TUNEL assay also revealed the apoptotic induction of the HL-60 cells treated with ACE. To obtain further information on the ACE-induced apoptosis, the expression level of certain apoptosis-associated proteins was examined using a Western blot analysis. Treatment of the HL-60 cells with ACE resulted in the activation of caspase-3, and subsequent proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). The above results confirmed that the apoptosis in the HL-60 cells was induced by ACE, and that caspase-3-mediated PARP cleavage was involved in the process.

• Journal of Ginseng Research
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• v.37 no.2
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• pp.227-247
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• 2013

MicroRNAs (miRNAs) are a class of recently discovered non-coding small RNA molecules, on average approximately 21 nucleotides in length, which underlie numerous important biological roles in gene regulation in various organisms. The miRNA database (release 18) has 18,226 miRNAs, which have been deposited from different species. Although miRNAs have been identified and validated in many plant species, no studies have been reported on discovering miRNAs in Panax ginseng Meyer, which is a traditionally known medicinal plant in oriental medicine, also known as Korean ginseng. It has triterpene ginseng saponins called ginsenosides, which are responsible for its various pharmacological activities. Predicting conserved miRNAs by homology-based analysis with available expressed sequence tag (EST) sequences can be powerful, if the species lacks whole genome sequence information. In this study by using the EST based computational approach, 69 conserved miRNAs belonging to 44 miRNA families were identified in Korean ginseng. The digital gene expression patterns of predicted conserved miRNAs were analyzed by deep sequencing using small RNA sequences of flower buds, leaves, and lateral roots. We have found that many of the identified miRNAs showed tissue specific expressions. Using the insilico method, 346 potential targets were identified for the predicted 69 conserved miRNAs by searching the ginseng EST database, and the predicted targets were mainly involved in secondary metabolic processes, responses to biotic and abiotic stress, and transcription regulator activities, as well as a variety of other metabolic processes.

• Journal of Convergence for Information Technology
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• v.10 no.7
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• pp.249-256
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• 2020

To investigate the natural cosmetic ingredients of Benincasa hispida seed extract on skin care, we measured anti-oxidant and anti-inflammatory, and whitening effect. DPPH free radical scavenging activity was increased in a dose-dependent manner. The total phenolic content was higher in methanol extract (22.42±0.002 mgGAE/g) than water extract (9.77±0.002 mgGAE/g). MTT assay was demonstrated that the seed extract did not have a cytotoxic effect in RAW264.7 and B16F10 cell lines. We also examined to find out the inhibitory activity on NO production and secretion of TNF-α cytokine in LPS-induced RAW264.7 cells. In B16F10 melanoma cells, the seed extract significantly suppressed α-MSH induced melanin synthesis. Furthermore, westernblot analysis revealed that methanol extract dramatically downregulated the expression level of MITF, TRP-1 and TRP-2. Taken together, the B. hispida seed extract posses anti-oxidant, anti-inflammatory and skin whitening activities, which might provided its functional efficacy in cosmetic materials.

• Journal of Convergence for Information Technology
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• v.10 no.11
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• pp.272-278
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• 2020

This study was conducted to confirm the component and efficacy evaluation of Buxus Koreana by different part. Leaf, stem, root, and fruit were extracted with 70% ethanol, and the contents of polyphenols and flavonoids were analyzed, and the efficacy against anti-inflammatory, moisturizing and wrinkles were confirmed. The contents of polyphenols and flavonoids of B. Koreana were the highest in fruit extracts at 77.32 and 51.91 mg/g, respectively. As a result of confirming the NO production inhibitory effect, the root extracts showed an inhibitory effect of 29.6%, and iNOS significantly decreased as the concentration of the extracts increased compared to the LPS treatment group. As a result of checking the mRNA expression level of MMP1, it was found to be effective in the root and stem extracts. Through the efficacy evaluation of B. Koreana the possibility of use as a cosmetic material was confirmed.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.49 no.2
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• pp.61-74
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• 2012

In this paper, we propose a illumination-robust face recognition system using MCS-LBP and 2D-PCA algorithm. A binary pattern transform which has been used in the field of the face recognition and facial expression, has a characteristic of robust to illumination. Thus, this paper propose MCS-LBP which is more robust to illumination than previous LBP, and face recognition system fusing 2D-PCA algorithm. The performance evaluation of proposed system was performed by using various binary pattern images and well-known face recognition features such as PCA, LDA, 2D-PCA and ULBP histogram of gabor images. In the process of performance evaluation, we used a YaleB face database, an extended YaleB face database, and a CMU-PIE face database that are constructed under varying lighting condition, and the proposed system which consists of MCS-LBP image and 2D-PCA feature show the best recognition accuracy.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.44 no.4 s.316
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• pp.48-54
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• 2007

Hand shape is one of important elements in Korean Sign Language (KSL), which is a communication method for the deaf. To express sign motion in a virtual reality environment based on OpenGL, we need an editor which can insert and modify sign motion data. However, it is very difficult that people, who lack knowledge of sign 1anguage, exactly edit and express hand shape using the existing editors. We also need a program to efficiently construct and store the hand shape data because the number of data is very large in a sign word dictionary. In this paper we developed a KSL hand shape editor to easily construct and edit hand shape by a graphical user interface (GUI), and to store it in a database. Hand shape codes are used in a sign word editor to synthesize sign motion and decreases total amount of KSL data.

• JSTS:Journal of Semiconductor Technology and Science
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• v.11 no.4
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• pp.262-271
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• 2011

In this work, we present a unified analytical surface potential model, valid for both PD and FD SOI MOSFETs. Our model is based on a simplified one dimensional and purely analytical approach, and builds upon an existing model, proposed by Yu et al. [4], which is one of the most recent compact analytical surface potential models for SOI MOSFETs available in the literature, to improve its accuracy and remove its inconsistencies, thereby adding to its robustness. The model given by Yu et al. [4] fails entirely in modeling the variation of the front surface potential with respect to the changes in the substrate voltage, which has been corrected in our modified model. Also, [4] produces self-inconsistent results due to misinterpretation of the operating mode of an SOI device. The source of this error has been traced in our work and a criterion has been postulated so as to avoid any such error in future. Additionally, a completely new expression relating the front and back surface potentials of an FD SOI film has been proposed in our model, which unlike other models in the literature, takes into account for the first time in analytical one dimensional modeling of SOI MOSFETs, the contribution of the increasing inversion charge concentration in the silicon film, with increasing gate voltage, in the strong inversion region. With this refinement, the maximum percent error of our model in the prediction of the back surface potential of the SOI film amounts to only 3.8% as compared to an error of about 10% produced by the model of Yu et al. [4], both with respect to MEDICI simulation results.