• KSBB Journal
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• v.19 no.3
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• pp.174-177
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• 2004

A method of collection and long-term storage of viable lily (Lilium longiflorum) pollen grains were developed for their in vitro growth and transformation in consistency. Petroleum ether, n-heptane, cyclohexane and benzene, as pollen collection medium, were determined less toxic to pollen growth in vitro than others tested. Pollen grains, however, lost their growth activity if stored in these solvents more than a week, So, a serial performance, that is, pollen grain collection in these solvents, air-drying and immediate transfer to low temperature condition was determined desirable for keeping the viability much longer. Pollen grains from this storage showed a successful transformation in vitro with a cDNA encoding tissue plasminogen activator (TPA) protein using Agrobacterium via vacuum infiltration according to western blotting analysis.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.181-181
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• 2017

Low impact development (LID) practices has become important to mitigate the damage from natural disasters in urban areas. Thereby many hydrological simulation models can simulate the hydrological impact of LID practices. However, commonly used models are not able to provide specific information to most users such as where LIDs should be placed and what kind of LID should be designed. In this study, a decision support system which can be used with the EPA's SWMM was developed for the determination of LID types and locations of LID practices, named Water Management Prioritization Module (WMPM), was applied to a urbanized university campus. Eight sub-catchments were selected as feasible candidate areas for the planning of LID practices. Pre-designated infiltration trenches and permeable pavements were applied to each selected sub-catchments, followed by peak and total runoffs comparison between before/after planning of LIDs. Moreover, TOPSIS, one of a multi-criteria decision analysis method was used in the procedure of selecting target sub-catchment areas and final prioritization of LID types and locations. As a result, sub-catchments S4 with permeable pavements and S16 with infiltration trenches has shown the most decrease in total and peak runoffs, respectively. Therefore, WMPM was found to be effective in determining the best alternative among various scenarios generated and simulated.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.23 no.1
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• pp.1-7
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• 2010

Objective : Mercuric chloride is excreted in the urine and stool. Bangpungtongseong-san(BT) has been used commonly skin disease and has diuresis and excretion effect. This study is aimed to find out effects of Kami- bangpungtongseong-san(KBT) on the skin disease toxicated by mercury. Method : Experiment was conducted with No treated group(Normal group), Mercuric chloride subcutaneous injection group(Control group) and Kami-bangpungtongseong-san-treated group (Sample group). KBT Extracts were delivered orally in 7 days in sample group. We observed epithelial cell hyperplastic, angiogenesis, inflammatory cell infiltration of skin. For the charting the results, image analysis was taken. The result of image analysis was verified significance by Sigmaplot 2000(P<0.05). Result : This study shows an relieving epithelial cell hyperplastic, angiogenesis, inflammatory cell infiltration of exposure skin on mercuric chloride. Conclusion : According to the result of study, we can expect to the effect of KBT extracts' therapeutic action to tissue injuries of the mice' skin on acute mercurial toxication.

• Journal of Korea Water Resources Association
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• v.37 no.3
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• pp.249-255
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• 2004

To investigate the return flow ratio of irrigation water, lots of observations were made during the irrigation periods in 2003 crop year. This Area is a portion of Dae-Am pumping station basin which is located in Changryung-gun, Gyeongnam province. A water balance analysis was performed for a paddy field in Dae-Am pumping station in the Nakdong river basin, which is constructed for irrigation water supply. Daily rainfall data in the this area were collected and irrigation water flow rate, drainage water flow rate, infiltration and evaportranspiration were measured in field area. Irrigation water flow rate and drainage water flow rate were continuously observed by water level logger(GTDL-L10) during the growing season. The infiltration and evaportranspiration were measured by cylindrical 300mm depletion meter and cylindrical 200mm infiltrometer, respectively. Total irrigation and drainage flows were 654.7mm and 281.2mm in 2003. Total infiltration and evaportranspiration were 36.0mm and 160.0mm respectively. The mean of the daily evaportranspiration rate was 4.3mmm/d. The prompt return flow and retard return flow ratio were 43.0％ and 5.5％, respectively. Total return flow ratio was 48.5％. Therefore, it can be concluded that the amount of irrigation water was much higher than design standard or reference in this study. It means that this was caused by the inadequate water management practice in the area where water was oversupplied on farmers' request rather than following sound water management principles, and design standard should be changed in the future.

• Journal of Advanced Marine Engineering and Technology
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• v.30 no.2
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• pp.267-274
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• 2006

There are no solar loads through windows and heat transfer from outer walls of the building to the interior This study analyzes indoor air temperature and air flow distribution for the interior of buildings or vessels occupying space. Numerical method is adopted to visualize the indoor side environmental situation. that is without heat transmission to outside the building in various cooling load conditions; supply ventilation lighting. occupancy and infiltration in conditioned spaces. Reaching time of an air conditioner is predicted theoretically by cooling load variations. Theoretical modelling of the reaching time shows good agreement with experimental results.

• Journal of the Korea Institute of Military Science and Technology
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• v.3 no.2
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• pp.244-249
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• 2000

Thermal gradient chemical vapor infiltration (TG-CVI) process, which is one of the CVI techniques to densify a porous fiber preform, requires for a heater to have uniform surface temperature distribution. Thus, it is essential to design the shape of the heater and to predict the temperature distribution when the heater has a profile which is not a simple cylinder. In this study, an analytical method has been used to design the inner profile of a conical heater showing uniform temperature distribution, if its outer shape is specified. Temperature distribution on the heater surface has been calculated with the finite difference method and compared with the experimental results. When a heater had a combined profile with a large cone and a small cylinder, temperature was higher in the cylindrical part. To reduce the temperature difference between these areas, a hole-machining method has been proposed including other possible ones. A shape design and optimization program has been made to improve the temperature uniformity of the TG-CVI heater better than that designed with the analytical method.

• Journal of the Korean Society of Groundwater Environment
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• v.6 no.1
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• pp.23-32
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• 1999

A new numerical method using solution-adaptive grids (SAG) is developed to solve the Richards' equation (RE) for unsaturated flow in porous media. Using a grid generation technique, the SAG method automatically redistributes a fixed number of grid points during the flow process, so that more grid points are clustered in regions of large solution gradients. The method uses the coordinate transformation technique to employ a new transformed RE, which is solved with the standard finite difference method. The movement of grid points is incorporated into the transformed RE, and therefore all computation is performed on fixed grid points of the transformed domain without using any interpolation techniques. Thus, numerical difficulties arising from the movement of the wetting front during the infiltration process have been substantially overcome by the new method. Numerical experiments for an one-dimensional infiltration problem are presented to compare the SAG method to the modified Picard method using a fixed grid. Results show that accuracy of a SAG solution using 41 nodes is comparable with the solution of the fixed grid method using 201 nodes, while it requires only 50% of the CPU time. The global mass balance and the convergence of SAG solutions are strongly affected by the time step size (Δt) and the weighting parameter (${\gamma}$) used for generating solution-adaptive grids. Thus, the method requires automated readjustment of Δt and ${\gamma}$ to yield mass-conservative and convergent solutions, although it may increase computational costs. The method can be effective especially for simulating unsaturated flow and other transport problems involving the propagation of a sharp-front.

• Journal of Korea Foundry Society
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• v.20 no.5
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• pp.300-306
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• 2000

The objective of this work was to investigate the effects of SiC particle size(50, 100 ${\mu}m$) and additional elements such as Si, Cu and Ti on aging behavior in Al-5Mg-X(Si,Cu,Ti)/SiCp composites fabricated by pressureless infiltration method using hardness and wear test, scanning electron microscopy(SEM) and differential scanning calorimetry(DSC). The peak aging time in Al-5Mg-X(Si, Cu, Ti)/SiCp(50, 100 ${\mu}m$) composites is shorter than Al-5Mg-0.3Si alloy.The peak aging time of 50 ${\mu}m$ SiC particle reinforced Al-5Mg-X(Si,Cu,Ti) composites is shorter than those of 100 ${\mu}m$ SiC particle reinforced of Al-5Mg-X(Si,Cu,Ti) composites. The Al-5Mg-0.3Si-0.1Cu-0.1Ti/SiCp(50 ${\mu}m$) composites aged at $180^{\circ}C$ has higher hardness and better wear resistance than any other aged composite.The aging effect is promoted by the addition of Si and Cu in Al-5Mg/SiCp composites, so the wear resistance of Al-5Mg/SiCp composites with Si and Cu elements is enhanced by the aging treatment.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.4
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• pp.968-980
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• 1995

Al/Al$_{2}$O$_{3}$/C hybrid metal matrix composites are fabricated by the direct squeeze infiltration method. From the microstructure of Al/Al$_{2}$O$_{3}$/C composites, uniform distribution of reinforcements and good bondings are found. Optimum processing conditions for preforms and squeeze castings are suggested. Mechanical properties, such as elastic modulus, elongation, 0.2% offset yield strength and ultimate tensile strength are obtained. Through the abrasive were test and wear surface analsis, wear behavior and its mechanism of AC2B aluminum and Al/Al$_{2}$O$_{3}$/C composites can be characterized under various sliding speed conditions. Tensile strenght elongation of Al/Al$_{2}$O$_{3}$/C composites are decreased with increasing the addition of carbon fiber. On the contrary, elastic modulus of Al/Al$_{2}$O$_{3}$/C composites is slightly improved compared with that of the unreinforced matrix alloy. The addition of carbon fiber to al/al$_{2}$O$_{3}$/C composites gives rise to improvement of the wear resistance. Specially, carbon chopped fibers play an important role in interfering sticking between the counter material and metal matirix composites. Al/Al$_{2}$O$_{3}$/C composites are suitable to high speed due to solid lubication of carbon. And wear model of Al/Al$_{2}$O$_{3}$/C composites is suggested by the examination of worn surfaces.

• Applied Microscopy
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• v.28 no.3
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• pp.315-328
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• 1998

The present study was carried out to investigate the effect of ultrasonic bath in tissue preparation for transmission electron microscopy. The method used standard reagents and media, and employed ultrasonic bath agitation to accelerate fluid exchange. The liver kidney, stomach and cardiac muscle tissues of male Sprague-Dawley rats were used for the experiment, and the experimental design was divided into 4 groups; The control group using rotators (Traditional method, 1,625 mins) and the three experimental groups using ultrasonic bath (UB) in the primary fixation through the infiltration processes (UB I; 62.5 mins, UB II; 125 mins, UB III; 250 mins). The results were as follows; 1. In the control group, tissues were easily sectioned, and showed well preserved intact membranes, and cell organelles such as mitochondria, lysosome, peroxisome, rough endoplasmic reticulum and smooth endoplasmic reticulum. 2. In the UB treated group I, tissues showed holes due to the inadequate removal of both water and fluids used in the dehydration process. Also the mitochondria of cell organelles, especially, showed swollen intracristal spaces and dense matrices due to poor fixation. 3. In the UB treated group II, tissues showed good preservation of cell organelles and specimen slice sections. Also, no holes were observed. 4. In the UB treated group III, tissues showed leaching of structural components in the cytoplasm, but no holes were observed. In conclusion, the ultrasonic bath procedure takes approximately 120 minutes from specimen fixation to resin infiltration and gives excellent results.