For the detection of Cwptospori,mum oocysts, fecal samples were collected from 201 calves which showed diarrhea. Among the 201 samples, 29 samples (14.4%) were positive for Cwptosporinium spry. by the DMSO-modified acid-fast stain (MAFS) , 23 samples (11.4%) were positive by commercial kit (Meridian Diagnostics, Cincinnati, Ohiol and 23 by the indirect immunofluorescence antibody (IFA )assay employing the monoclonal antibody (mAb C6). When tested by both IFA and MAFS, 20 fecal samples were positive for Cwptosporinium oocysts whereas 169 fecal samples were negative. If the MAFS is considered a standard method for oocyst detection, the IFA showed 69% of sensitivity and 98% of specificity. When tested by both IFA and commercial kit, 22 fecal samples were positive for Cwptospori,mum oocysts while 177 samples were negative. One sample tested by IFA was found to be false negative, when compared with the results by commercial kit. The sensitivity of IFA was calculated as high as 96%; the specificity as 99% and the predictive value was also 99%. In the present study, IFA employing the nAb C6 revealed that 23 samples (11.4%) were positive among the 201 calves showing diarrhea. Of 23 IFA positive samples, 4 samples (5%) showed cryptosporidial oocysts more than 105 OPG Therefore. it is concluded that the calves showing cryptosporidial oocysts more than 105 OPG in the feces were highly associated with clinical cryptosporidiosis.
Hand washing is an important component of hygiene program for food handlers. Hands can be a source of direct or indirect contamination of foods with pathogenic microorganisms. In this study, the effectiveness of hand washing methods and the use of 70% alcohol solution against transient skin bacteria was tested in an university foodservice facility. 70% alcohol solution is sprayed for 5 seconds automatically when hands are placed in the dispenser. Samples were taken using swab technique in meat cutting area, vegetable trimming area, and vegetable cutting area: before and after washing hands according to planned methods, and after being sprayed with 70% alcohol solution after washing hands. The bacteriological analysis of total plate counts, coliform, fecal coliform of food handlers' hands was done. Statistical data analysis was completed with Mann-Whitney U test and Kruskal-Wallis model using the SPSS program. The levels of initial contamination of workers' hand were significantly different by the work areas($x^2$=9.156, p<0.01). Workers in the vegetable trimming area had more heavily soiled hands than in the other work areas. Mean of TPC counts and coliform was 8.97×$10^5$ CFU/12.4$cm^2$, 2.93×$10^2$ MPN/12.4$cm^2$ respectively, but fecal coliform was not detected. Transient bacteria were removed from hands after washing and using 70% alcohol solution but were not removed completely. Mean reduction percentage in TPC varied among work areas and ranged from 93.19% to 94.99%, and in coliforms from 97.31% to 100%. A significant difference in TPC was found between before and after hand disinfection (Z=-2.714, p<.01) and between standardized hand washing procedures and un-standardized hand washing procedures(z=-2.301, p<.01). Subjects using the hand sanitizer showed a great elimination of TPC(99.45% reduction), but this effect was valid only after following proper washing procedures. Based on the results, the most effective hand washing method was recommended as the combination of the standardized hand washing procedure with warm-water and use of the 70% alcohol solution. The results can be used to develop hand hygiene programs and training strategies for enhancing hand hygiene practices for food handlers in foodservice operations.
One of water and/or methanol extracts from 14 herbal deugs which were screened using murine splenocytes showed immunosuppressive activities previously. After water extract from Xanthium strumarium was treated with chloroform. $100 \mu{g/ml}$ of water layer (XS-WCI) has very strong immunosimulating activities tested by $^3H$-thmidine incorporation (control as $100 \mu{g/ml}$, 26345 cpm was 69515 cpm). MLR also appears to be simulated strongly (control vs $100 \mu{g/ml}$, 4962 cpm vs 78688 cpm). When $100 \mu{g/ml}$ of XS-WCI and $0.8 \mu{g/ml}$ of concanavalin a (ConA) were added. more $^3H$-thymidine were incorporated significantly, compared with $0.8 \mu{g/ml}$ of ConA only. In contrast with ConA. results from $5 \mu{/ml}$ of lipopolysaccharide (LPS) and $100 \mu{g/ml}$ of XS-WCI were not different. compared with $5\mu{/ml}$of LPS only. These results indicated the responses of XS-WCI to B cell and T cell may be different. XS-WCI was injected intraperitoneally (10 mg/kg. 50mg/kg/ 100 mg/kg) for 4 days or 10 days and tested secretion of IgM or IgG by direct and indirect hemolytic plaque-forming cell assays, respectively. Numbers of hemolytic plaques for both IgM and IgG were increased significantly. Especially, secretion of IgGs was increased more than 10 times. After administration of XS-WCI for 7 days (50 mg/kg. 100 mg/kg) splenomegaly deu to graft vs host reaction was observed. Human lymhocytes separated from whole blood by Ficoll-Hypaque method were also proliferated after treatment of $10 \mu{g/ml}$ and $50 \mu{g/ml}$ of XS-WCI. As seen in murine lymphocytes, human lymphocyte proliferation was increased synergistically after treatment with both of XS-WCI and phytohemagglutinin (PHA). It appears that XS-WCI may have potential immunosimulating activities and that it remains to be purified further for isolation of active components.
Journal of rehabilitation welfare engineering & assistive technology
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v.9
no.4
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pp.275-283
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2015
This study was to find out the relationship between the rehabilitation service and the life satisfaction of industrially injured workers and how medical rehabilitation service, psycho-social rehabilitation service, and occupational rehabilitation service influence in this relation. For this, it analyzed 977 people who were using rehabilitation service with industrial disaster disability among 2,000 research subjects using the first year materials of industrial disaster insurance panel of Workers' Compensation & Welfare Service. For the relationship of variables on the suggested 3 models, the mediating effect of the occupational rehabilitation service in the relation of medical rehabilitation of the industrially injured workers and life satisfaction by applying SPSS Macro and Sobel method and did the regression analysis to find out the control effect of psycho-social rehabilitation service by the mean centering of the material. The result of the analysis showed; when the satisfaction on the medical rehabilitation service was increased the satisfaction on the voacational rehabilitation was increased, when the satisfaction on the occupational rehabilitation service was increased, the satisfaction on the life satisfaction was increased, and when the satisfaction on the psycho-social rehabilitation service was increased the medical rehabilitation service had a indirect effect on the daily life satisfaction.
Journal of the Society of Cosmetic Scientists of Korea
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v.46
no.3
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pp.307-317
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2020
The barrier structure of the skin's epidermis is a key structure to prevent the loss of water inside the body and the invasion of foreign substances, and is composed of keratinocytes and intercellular lipids. At this time, the intercellular lipids of the skin barrier has the strongest structure when packed in an orthorhombic structure. However, it is damaged by various external causes and changes to a hexagonal structure. This change in physical structure can be analyzed non-invasively by analyzing the signal of the CH2-CH2 scissoring band of lipids using FT-IR. In this study, SDS was treated on porcine skin to construct a skin barrier damage model, and the degree of change in packing structure was quantified by analyzing FT-IR signals. We then judged whether the barrier of the damage model was recovered according to the treatment of the cosmetic formulation. From these results, an indirect method of measuring the water evaporation of the skin barrier to date can be supplemented. In addition, physical changes in the structure of the skin barrier can be utilized in a direct and efficient manner to identify the function and verify the formulation of various materials.
Im, Seon yeong;Jeon, Young ji;Mun, Se young;Han, Kyu dong;Ahn, Tae young;Lee, Dong jin
Proceedings of the Korean Society of Crop Science Conference
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2017.06a
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pp.350-350
/
2017
The study was conducted to evaluate differences of growth characteristics of rice cultivated in two different regions (Cheonan and Jeonju). It focused on nutritional composition and anti-nutritional factors of rice straw produced from 21 rice varieties including GM rice (Iksan 483). The range of general nutrition ingredient is that crude was 0.97 ~ 3.2 %, carbohydrate was 67.45 ~ 80.01 %, crude protein was 1.46 ~ 4.81 %, crude ash was 6.52 ~ 18.96 %, crude fiber was 25.77 ~ 40.02 %, NDF was 51.84 ~ 67.77 %, ADF was 27.11 ~ 40.44 %, calcium was 0.49 ~ 5.18 mg/g and phosphorous was 0.26 ~ 2.77 mg/g. The general nutritional contents of GM rice were included above range. The range of phytic acid of rice straws cultivated in Cheonan and Jeonju was 0 ~ 0.056 mg/ml and 0 ~ 0.059 mg/ml, respectively. The phytic acid content of GM was 0.033 mg/ml, which was in the range of the content of rice straw in Cheonan and Jeonju. The range of trypsin inhibitor of rice straws cultivated in Cheonan and Jeonju was 0.061 ~ 0.461 TIU/mg and 0 ~ 1.278 TIU/mg, respectively. The trypsin acid content of GM was 0.461 TIU/mg, which was in the range of the content of rice straw in Cheonan and Jeonju. In addition, we investigated microbial community from each soil sample by using metagenomics sequencing based on rRNA microbial diversity in order to inspect indirect changes of soil environment with cultivation of GM rice. Metagenomics analysis was carried out using soil samples cultivated with GM and non-GM rice for before transplanting, young panicle differentiation stage, heading stage, and ripening stage. Beta diversity of microbial community in both soil environments were calculated by using Bray-Curtis distance method and showed low value with an average of 0.24 (dissimilarity = 1). As a result, it was confirmed that the cultivation of GM does not give a significant effect on the change of microbial composition in soil. Therefore, Our study demonstrates that there is no difference in the composition of soil microorganism due to GM and non-GM rice.
This study was conducted to manufacture a good qualified organic fertilizer with cow dung through rapid composting process, and the proposal guideline of the degree of maturity could be estimated with the measurement of final product. It included total nitrogen content of above 2% on an oven-dry material basis, C/N ratio of below 20, CEC of more than about 60 me/100g, ratio of carbon in reducing sugar to the total carbon of below 35%, and temperature in pile of above $60^{\circ}C$. The total nitrogen content, the C/N ratio, and the Paper Chromatographic method couldn't be the guideline to evalute the maturity of cow dung compost. CEC was increased in increased fermentation and it was high in the high fermented temperature plots which were cow dung+ shredded bark in 1988, cow+dung+wood chips in 1989, and cow dung+rice straws in 1990. The ratio of carbon in reducing sugar to total carbon in 1990 was lower in cow dung+saw dust than cow dung+rice straws that was the highest temperature in pile. Generally cow dung was mixed well with saw dust and thus the total carbon of the product was high. The measurement of the temperature in pile seems to be a indirect guideline of maturity.
The OCB(organizational citizenship behavior), spontaneous extra-role behavior of social workers, plays very important role in the provision of qualitative service for the clients and the organizational performance of social welfare agencies. Starting from the importance of OCB for the effective organizational management, this study focuses on the effects of antecedents such as organizational justice(procedural and distributional) and trust in supervisor on the OCB. This study collected data from 360 social workers and 59 their supervisors working in the community welfare centers, paired the self-reported data and the supervisor-rated data, and analyzed the data through the SEM (Structural Equation Modeling) method. The findings of the study show that organizational justice(procedural and distributional) has no direct effect on the OCB, but indirect effect on the OCB through the mediator of trust in supervisor, and that procedural justice has more effect than distributional justice. This study suggests that the management of spontaneous behavior of social workers is not a separated fragmentary phenomenon but should be understood and managed comprehensively in the continuation of the perception of organizational members for the organizational management, the resulting affectivity toward their supervisor, and their behavior in the organizational life.
Kim, Eun-Jeong;Bae, Kwang-Soo;Ahn, Gye-Hyeong;Lee, Chul-Ki
The Journal of The Korea Institute of Intelligent Transport Systems
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v.9
no.6
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pp.13-21
/
2010
Road Traffic Authority and National Police Agency is currently managing the "development of an integrated metropolitan traffic information infrastructure" that installs of facilities for a traffic information infrastructures, such as Local Traffic Information Center, UTIS(Urban Traffic Information System), CCTV, VMS and etc. CTIC was established in 2005 to act as an traffic information hub and to provide integrated UTIS traffic information without regional barriers. This study was carried out to seek for solution to improve quality of UTIS traffic information service for the Central Traffic Information Center(CTIC). In this study, the Evaluation index for UTIS traffic information service was developed and the implementation plan was established by using requirement analysis method, case study and AHP(Analytic Hierarchy Process) technique. The Evaluation index consist of 5 fields and 20 index, it make possible evaluation of direct/indirect effect for UTIS traffic information service. In conclusion, It is expected that quality of UTIS traffic information service will be improved by using developed evaluation index and also can be applied for performance evaluation of other traffic information systems.
Asia-Pacific Journal of Business Venturing and Entrepreneurship
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v.15
no.5
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pp.65-76
/
2020
This study analyzed the impact of women's self-preservation on their intention to start a business by using self-efficacy as a mediation, reflecting the importance of women's self-preservation to adapt to the new environment of start-up. In particular, in addition to the overall self-preservation, the sub-factors of behavioral delay, compensatory natural strategy, and constructive thinking strategy were also validated. As a research method, 198 people who responded to the online survey were analyzed using Model 4 of the PROCESS macro proposed by Hayes(2018), and indirect effects were analyzed by designating 5,000 bootstrapping samples. The findings were as follows. First, self-efficacy has been shown to have a mediating effect in which women's self-preservation influences their intention to start a business. At this time, the medium effect was shown as a partial medium. Second, the sense of self-efficacy was found to have a mediating effect on women's behavior-oriented strategies affecting their intention to start a business. At this time, the medium effect was shown as a partial medium. Third, self-efficacy was found to have a mediating effect in which women's natural compensatory strategies affect their intention to start a business. At this time, the medium effect was shown as a partial medium. Fourth, self-efficacy was found to have a mediating effect in which women's constructive thinking strategies affect their intention to start a business. At this time, the medium effect was shown as a complete medium. This shows that the higher a woman's self-preservation, the higher her self-efficacy and willingness to start a business. Based on the results of these studies, he made a developed proposal for women's start-ups.
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