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Diversity of Aspergillus, Penicillium, and Talaromyces Species Isolated from Freshwater Environments in Korea

  • Heo, Inbeom;Hong, Kyeongyeon;Yang, Hyejin;Lee, Hyang Burm;Choi, Young-Joon;Hong, Seung-Beom
    • Mycobiology
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    • v.47 no.1
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    • pp.12-19
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    • 2019
  • In order to elucidate the fungal diversity and community structure in freshwater environments, numerous fungal strains were isolated from freshwater, submerged soils, twigs, dead insects, etc. Among them, the present study has focused specifically on Aspergillus, Penicillium, and Talaromyces species, which produce diverse useful metabolites in general. Twelve strains of Aspergillus isolated were identified as A. japonicus (n = 5), A. tubingensis (3), A. niger (2), and A. flavus (2), 10 strains of which belong to Aspergillus section Nigri, named black Aspergillus. Eight strains of Penicillium were identified as P. brasilianim (n = 3), P. oxalicum (2), P. crustosum (1), P. expansum (1), and P. piscarium (1). Two different strains of Talaromyces were identified as T. pinophilus and T. versatilis. Thus far, Penicillium piscarium and Talaromyces versatilis have been unrecorded in Korea, for which we provide detailed morphological and molecular characteristics.

Current Taxonomical Status of Korean Auricularia Species

  • Cho, Sung-Eun;Kwag, Young-Nam;Lee, Dong-Hyeon;Han, Jae-Gu;Kim, Chang Sun
    • The Korean Journal of Mycology
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    • v.49 no.1
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    • pp.21-31
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    • 2021
  • Auricularia (Auriculariaceae, Basidiomycota) is well-known jelly fungi, and most species are cultivated or utilized medicinally around the world. A total of 127 Auricularia specimens were collected during Korean mushroom forays by the Korea National Arboretum from 2008 to 2020. In this investigation, fungal barcode analysis and morphological examinations of 33 specimens were conducted and five Korean Auricularia species were identified. Of the species identified, A. americana, A. heimuer, and A. villosula were new to Korea, while A. auricula-judae which was previously identified in Korea, was not found. Herein, we discuss the Korean Auricularia species and provide a taxonomic key for their morphological identification.

A novel RET mutation identified in a patient with pheochromocytoma and renal cell carcinoma

  • Kwon, Jae Wan;Jung, Eui Dal;Jeon, Eon Ju;Park, Jung Kyu;Lee, Joon Kee;Cho, Chang Ho
    • Kosin Medical Journal
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    • v.33 no.3
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    • pp.446-453
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    • 2018
  • Pheochromocytomas might be sporadic or genetic. Genetic pheochromocytoma is associated with multiple endocrine neoplasia (MEN) type 2A, MEN type 2B, and von Hippel-Lindau (VHL) disease. RET mutations are identified in more than 90% of index cases of MEN2 and familial medullary thyroid cancer and in about 4-12% of apparent sporadic cases. Here, we report a 54-year-old man presenting with pheochromocytoma and renal cell carcinoma, who was identified as having a novel missense RET mutation.

New record of three oxytrichid ciliates (Ciliophora: Oxytrichidae) from South Korea

  • Kyu-Seok, Chae;Gi-Sik, Min
    • Journal of Species Research
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    • v.11 no.4
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    • pp.287-295
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    • 2022
  • Three oxytrichid ciliates, Oxytricha lithofera Foissner, 2016, Pleurotricha curdsi (Shi et al., 2002) Gupta et al., 2003 and Sterkiella tetracirrata Kumar et al., 2015, were isolated from soils and confirmed as new to South Korea. Oxytricha lithofera was identified based on lithosomes, cortical granules, 21-33 adoral zone membranelles, one left (14-21 cirri) and one right (15-18) marginal rows and 5 transverse cirri. Pleurotricha curdsi was identified based on the possession of 2 macronuclear nodules, 2-3 micronuclei, 46-53 adoral zone membranelles, 3 frontal cirri, 5 frontoventral cirri, 5-7 postoral ventral cirri, 2-3 right marginal rows and 5 transverse cirri. Sterkiella tetracirrata was identified with respect 4 macronuclear nodules, 3-6 micronuclei, 25-40 adoral zone membranelles, 3 frontal cirri, 3 postoral ventral cirri, 2 pretransverse cirri, one left (21-30 cirri) and one right (24-30) marginal row and 4 transverse cirri. On the basis of 18S rDNA sequence analyses, we describe the phylogenetic positions of the three species.

Comparison of detective ranavirus with major capsid protein gene from infected frogs (Pelophylax nigromaculatus and Lithobates catesbeianus) in South Korea

  • Jongsun, Kim;Nam-Ho, Roh;Jaejin, Park;Daesik, Park
    • Journal of Ecology and Environment
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    • v.46 no.4
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    • pp.276-281
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    • 2022
  • Ranaviruses are a primary cause of amphibian extinctions. More consistent ranavirus-infection reports and genetic characterizations of identified viruses are urgently needed, particularly from Asian countries. The objectives of this study were to obtain the partial major capsid protein (MCP) gene sequences (506 bp) of the ranavirus responsible for infecting frogs in South Korea, as our previous research had confirmed using qPCR, and to evaluate their genetic relationships with other previously reported ranavirus sequences. Three different ranavirus MCP sequences were obtained from Pelophylax nigromaculatus and Lithobates catesbeianus. All six different types of MCP sequence from the ranavirus identified in South Korea to date belonged to the Frog virus 3 (FV3)-like virus group in the genus Ranavirus. To better understand the origin and spread of ranaviruses in South Korea, further infection reports and full genome analyses of the identified ranaviruses are needed.

Possibility and Challenge of Using Internet for International Exchange - Focused on Korean Students' Views -

  • Shin-hye, Heo
    • International Journal of Internet, Broadcasting and Communication
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    • v.16 no.1
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    • pp.55-62
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    • 2024
  • International exchange implementing the Internet began inevitably due to the pandemic, but it provided Digital Nomads with new experiences in their lives using the Internet. This study the possibility and challenge of international exchange implementing the Internet identified. To this end, we explored its strengths and weaknesses through interviews and descriptions of students who participated in international exchange. As a result, we identified that students were positive in the diverse aspects of communication tools and ways, whereas they were negative because they felt difficulties in the physical environment, inaccessible physical conditions especially. They were also negative in the emotional exchange, an ice-breaking which needed much more time than offline exchange. Therefore, we identified in the case of designing or conducting student activities implementing the Internet, including international exchange, the possibility of activities implementing the Internet could be much more extended if their developer various programs considered in the introduction step.

Whole-Genome Analysis of Salmonella Enterica subsp. Enterica serovar Gallinarum biovar Gallinarum Strain IJES3-1 Isolated from a Retail Chicken Shell Egg in Korea

  • Beom Soon Jang;Kun Taek Park
    • Journal of Food Hygiene and Safety
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    • v.39 no.4
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    • pp.353-355
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    • 2024
  • Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum causes fowl typhoid in poultry. In this study, we isolated Salmonella from a Korean retail chicken shell egg and performed whole-genome sequencing, from which we identified one chromosome (4,659,977-bp) and two plasmids (plasmid_1: 87,506 bp and plasmid_2: 2,331 bp). The isolate serotype was confirmed to be Gallinarum, with a biovar type of Gallinarum, which was finally identified as Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum. Multilocus sequence typing confirmed that the isolate was that of sequence type 78. The antimicrobial resistance gene, aac(6')-laa, was identified on the chromosome, and 166 virulence genes were detected on the chromosome and plasmid_1.

Floristic Study of Sangwangsan Mt. and Its Adjacent Areas(Wando-gun) (완도 상왕산 일대의 식물상 연구)

  • Gwang-Il Kim;Chan-jin Oh;Sun-jin Lee;Soon-Ho Shin;Kyoung-Pae Yun
    • Korean Journal of Environment and Ecology
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    • v.37 no.2
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    • pp.100-139
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    • 2023
  • This study was intended to identify the distribution and characteristics of plants such as native plants, rare plants, and endemic plants through a flora survey in Sangwangsan Mt. (644m), Wando-gun, Jeollanam-do, a group habitat of warm temperate forests in Korea, and use the data for the conservation of plant species diversity and the study of climate and distribution changes in warm-temperate forests. A total of 32 field surveys were conducted from 2018 to 2022. The survey identified 785 taxa, including 8 forms, 53 varieties, 16 subspecies, 708 species, 473 genera, and 132 families. The endangered wild plants designated by the Ministry of Environment included 6 taxa: Woodwardia japonica, Metanarthecium luteoviride, Bulbophyllum inconspicuum, Dendrobium moniliforme, Pelatantheria scolopendrifolia, and Cymbidium macrorhizon. Rare plants designated by the Korea Forest Service were identified as 26 taxa. The red list designated by the Korea National Arboretum was identified as 7 taxa, the red list designated by the Ministry of Environment was identified as 29 taxa, and endemic plants in Korea were identified as 17 taxa. Floristic target species in Korea were identified as 200 taxa, specifically 6 taxa of grade V, 13 taxa of grade IV, 73 taxa of grade III, 29 taxa of grade II, and 79 taxa of grade I. Naturalized plants were identified as 73 taxa, and invasive alien plants were identified as 6 taxa. Target plants adaptable to climate change in Korea were identified as 55 taxa, specifically 8 taxa of endemic plants, 46 taxa of southern plants, and 1 taxon of northern plants.

Availability of MADLDI-TOF MS for Identification of Gram Positive Bacilli Isolated from Blood Culture

  • Choi, Jin-Un;Kim, Sang-Ha;Hwang, Su-Jeong;Yu, Young-Bin;Kim, Sunghyun;Kim, Young-Kwon
    • Biomedical Science Letters
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    • v.24 no.2
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    • pp.108-115
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    • 2018
  • In the present study, results of the identification of Gram-positive bacilli (GPB) were analyzed by using the MALDI-TOF MS technique to score each 2-year blood culture at a university hospital. In addition, 16S rRNA sequence analyses and MALDI-TOF MS results are compared to targeting strains that had been isolated two or more times within the same patient, to evaluate the usefulness of MALDI-TOF MS in GPB identification. According to the cut-off (${\geq}1.7$) criteria, there were 410 (57.5%) reliable strains and 303 (42.5%) non-identified strains among the GPB identification results of 713 strains, using a microflex MALDI Biotyper (Bruker Daltonik GmbH, Bremen, Germany). The isolation appeared most often in the following order: Corynebacterium striatum, Bacillus cereus, Bacillus subtilis, Paenibacillus urinalis, and Listeria monocytogenes. Nearly three-fourths, 66 out of 89 (74.2%) of the strains for Corynebacterium striatum; 44 out of 60 (73.3%) strains for Bacillus cereus; and all (25 out of 25, 100%) Listeria monocytogenes strains were identified by their high scores of 2.0 or higher. Most (293 strains out of 303) non-identified strains were strains isolated only once and not significant as infectious bacilli. A total of 43 out of 50 (86.0%) strains matched and were able to be identified based on the 16 rRNA sequencing comparison results of strains that were isolated twice or more within the same patient and significant as infection bacilli. Non-matching among 5 out of 7 strains was not identified, even with MALDI-TOF MS. In conclusion, GPB can be identified in blood cultures using MALDI-TOF MS. This can be done accurately with ease, rapidly, and at a low cost. It is also thought to be helpful in GPB diagnosis and treatment.

The Flora of Mt. Biseul in Daegu (대구광역시 비슬산 일대의 식물상)

  • Kim, Jung-Hyun;Kim, Yong-Hyun;Yoon, Chang-Young;Kim, Joo-Hwan
    • Korean Journal of Environment and Ecology
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    • v.22 no.5
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    • pp.481-504
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    • 2008
  • This study was carried out to investigate the flora of Mt. Biseul. The vascular plants collected 10 times(from May 2006 to October 2007) were identified as 468 taxa in total, including 89 families, 302 genera, 397 species, 1 subspecies, 59 varieties and 11 forms. Korean endemic plants of this area were identified as 17 taxa including Thalictrum uchiyamai, Coreanomecon hylomeconoides, Lonicera subsessilis, etc.; the Ministry of Environment-designated plants, which should be protected by the wildlife protection law, were identified as one taxon including Aconitum austrokoreense; the Office of Forestry-designated rare & endangered plants were identified as 6 taxa including Viola albida, Lloydia triflora, Streptopus ovalis, etc.; the naturalized plants were identified as 34 taxa, and their naturalization ratio and urban index were found to be 7.3%, and 12.2% respectively. This study newly identified about 160 taxa of the plants, among which 17 taxa were newly added to the list of naturalized plants, such as Phytolacca americana, Dicentra spectabilis, Lepidium apetalum, Medicago lupulina, Euphorbia supina, Ambrosia artemisiifolia, Cosmos bipinnatus, Crassocephalum crepidioides, Galinsoga ciliata, Rudbeckia bicolor, Sonchus oleraceus, Tagetes minuta, Taraxacum officinale, Chloris virgata, Festuca arundinacea, Lolium perenne, Tradescantia reflexa, etc. There existed many kinds of plants resources having conservational value like Aconitum austrokoreense etc. in this area, but there remains a fear of nature destruction's acceleration due to indiscriminate human development and access of a lot of visitors, so it is judged that there should be a management plan, such as a limit on the number of visitors or rest-year-system for restoration of nature.