• 한국임상수의학회지
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• 제14권2호
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• pp.177-184
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• 1997

Canine parvovirus(CPV) is a very highly contagious virus causing hemorrhagic enteritis and myocarditis mainly in young dogs. The diseases were first recognized in 1978, and then spread throughout the world by 1980. The main source of the infection seems to be the feces of infected dogs, at the same time feces are suitable materials for detection of virus in the enteric form exactly for the same reasons. Recently, a new technique of in vitro DNA amplification, Known as the polymerase chain reaction (PCR), has been widely applied to clinical viral diagnosis because of its sensitivity, specificity and rapidity. In this research, we attemped to set up the PCR for the detection of CPV in fecal samples and conformed the canine parvpviral enteritis by PCR. To increase the sensitivity and specificity of a PCR, the nested PCR (two-step PCR) was performed. We also surveyed the contamination status of CPV in the research using fecal specimen was highly sensitive and specific. Of the 100 fecal specimens suspected canine parvoviral enteritis, 45 fecal specimens were positive in HA test, 64 fecal specimens were positive in the first PCR, and 87 fecal specimens were positive in the second PCR. CPV contamination status of animal clinics and breeding centers was serious, wo hygienic management of environment in which dogs are reared is required. The nested PCR described here seems to be a rapid, sensitive and specific for the detection of canine parvovirus.

• 한국동물위생학회지
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• 제40권2호
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• pp.119-131
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• 2017

Among different types of spoilage, microbial contamination can cause feed decomposition, which results in decreases in feed intake and productivity, infection, and breeding disorder. During the storage time, various microbes have a chance to inoculate with depreciation of feed and to infect the animals. We investigated bacteria that inhabit diverse feed ingredients and complete feed which have been stored for a few months. We isolated and identified 30 genera and 62 species of bacteria. Among these 62 species, 21 species were of non-pathogenic bacteria, 18 species were of pathogenic bacteria, 9 species were of opportunistic pathogens, and 14 species were of unknown bacteria. Pantoea allii and 24 species showed proteolytic enzyme activity. We also confirmed that 6 species including Pseudomonas psychrotolerans showed ${\alpha}$-amylase activity, and 29 species including Burkholderia vietnamiensis showed cellulase activity. Microbacterium testaceum and 3 species showed resistance to Ampicillin, Kanamycin, Streptomycin, Gentamicin, Carbenicillin, and Erythromycin ($50{\mu}g/mL$). Using mealworm larvae (Tenebrio molitor L.) as a model for pathogenicity, we confirmed that 8 species including Staphylococcus xylosus had pathogenicity for mealworm larvae. Especially, Enterobacter hormaechei, Staphylococcus xylosus, and Staphylococcus hominis were reported as being pathogenic for humans. This research suggests that hygienic management of animal feed is essential because beneficial and harmful bacteria can inhabit animal feed differently during storage and distribution.

• 한국환경보건학회지
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• 제35권1호
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• pp.36-44
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• 2009

Hand-washing has been recognized as a critical factor in controlling infection and cross-contamination. This study was performed to investigate hygienic behavior of male university students focusing on awareness of hand-washing, their hand washing practices, and the difference between their awareness and practices. Both a self-administered questionnaire survey and a separate direct observation in restrooms were carried out at a university campus over a four week period. In the survey, 93.6% of the responded students claimed to wash their hands after using toilet. However, just 16.9% of the observed students actually did so, according to a separate and unnoticed observational study. Among the observed students who washed their hands, 25.0% of them used soap, only 5.0% of them washed four parts of their hands, and 15.0% of them washed their hands for more than 10 seconds. Paper towel was the most common hand drying method in the direct observation and also in the survey. Significant differences between the questionnaire survey and the direct observation (p<0.0l) were found in hand-washing practices, duration of hand-washing, using soap, washing parts of the hand, and hand-drying method after using the toilet. This study indicates that there is a remarkable difference between the male university students' awareness of hand-washing and their hand-washing practices. This study reveals that there is the need for programs or campaigns to increase hand-washing practices of male university students.

• 농촌계획
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• 제9권2호
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• pp.13-17
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• 2003

A purpose of this study is to develop practical indexes for improving a village environment in Kyongbuk, Korea. From a questionnaire survey of the public employees (71 persons) who take charge of the environment in Kyongbuk province, the indexes were produced and the contents of questionnaire consisted of preferential and supplementary indexes to apply. The preferential indexes which apply to an agricultural village and a stock raising village are environmental sanitation facilities, a hygienic check of livestock and a purge of livestock shed's surroundings. In case of mountainous village, and natural-ecological village the preferential indexes are habitat reservation for wildlife, poaching prohibition (monitoring for it), and food supplement for livestock. To the traditional-cultural village, prevention of noise pollution, environmental sanitation facilities, and a monitoring or water quality change are the prior indexes in order. For the village near city and the industrial village the preferential indexes are patrol removing of garbage, pollution index planting and monitoring for noise pollution. For a fishing village and a village fronting waterside excess uses of agricultural chemicals, monitoring for a water quality change, and realignment of green house with vinyl and warehouse. In conclusion the research presents the practical and preferential index for residents to improve their environment in accordance with village settings and suggests guidelines for further research.

• 융합정보논문지
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• 제8권3호
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• pp.53-62
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• 2018

본 연구의 목적은 치주질환자의 치주질환에 대한 인식도 및 생활 스트레스와 구강건강관련 삶의 질과의 관련성을 파악하여 치주건강의 증진에 긍정적 기여를 할 수 있는 프로그램을 모색하고자 시도하였다. 연구대상은 치과 병 의원에 내원한 치주질환 환자 201명으로 하였고, 구조화된 무기명 자기기입식 설문조사를 실시하였다. 구강건강관련 삶의 질에 영향을 미치는 유의한 변수는 생활 스트레스, 치주질환 인식도, 구강위생용품, 수면량, 연령으로 나타났다. 이에 치주질환자들의 스트레스 관리능력 및 대처법의 중요함과 올바른 치주질환 인식도를 정립하기 위해 환자 상태별 적합한 상담이 필요할 것으로 사료된다.

• Journal of Periodontal and Implant Science
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• 제51권3호
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• pp.179-188
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• 2021

Purpose: Due to the difficulty of the hygienic care and sanitary management of abutment teeth and subpontic areas associated with fixed dental prostheses (FDPs), intrabony defects occur and accelerate due to the accumulation of plaque and calculus. This study aimed to evaluate the efficacy of regenerative periodontal surgery for intrabony defects associated with FDPs. Methods: The study inclusion criteria were met by 60 patients who underwent regenerative treatment between 2016 and 2018, involving a total of 82 intrabony defects associated with FDPs. Periodontal osseous lesions were classified as 1-, 2-, and 3-wall intrabony defects and were treated with an enamel matrix derivative in combination with bone graft material. The changes in clinical (pocket probing depth [PPD] and clinical attachment level [CAL]) and radiographic (defect depth and width) outcomes were measured at baseline and at 6, 12, and 24 months. Results: Six months after regenerative treatment, a significant reduction was observed in the PPD of 1-wall (P<0.001), 2-wall (P<0.001), and 3-wall (P<0.001) defects, as well as a significant reduction in the CAL of 2-wall (P<0.001) and 3-wall (P<0.001) intrabony defects. However, there was a significant increase in the CAL of 1-wall intrabony defects (P=0.003). Radiographically, a significant reduction in the depth of the 3-wall (P<0.001) defects and a significant reduction in the width of 2-wall (P=0.008) and 3-wall (P<0.001) defects were observed. The depth decreased in 1-wall defects; however, this change was not statistically significant (P=0.066). Conclusions: Within the limitations of the current study, regenerative treatment of 2- and 3-wall intrabony defects associated with FDPs improved clinical and radiological outcomes. Additional prospective studies are necessary to confirm our findings and to assess long-term outcomes.

• 대한영양사협회학술지
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• 제28권3호
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• pp.205-217
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• 2022

School meals are prepared based using the HACCP system, which is designed to ensure the physical and mental health of students. However, operational recommendations in school cafeterias have changed due to COVID-19 and include delays in serving time to restrict the number of meals and the installation of screens to prevent droplet transmission. Unfortunately, these changes may have detrimentally affected hygiene practices and generated new food poisoning sources. This study aimed to determine the hygiene state of school cafeterias in the Gyeongnam area from December 2020 to September 2021 based on the monitoring of total aerobic and coliform bacteria. Kitchen floors were the most contaminated areas with an average number of total aerobic bacteria of 4.3 log CFU/100 cm², whereas counts in dining areas were relatively low at 0.1~3.5 log CFU/100 cm². Newly installed partitioned areas had the lowest contamination level of 0.1~2.8 log CFU/100 cm². Escherichia coli was not detected on any surface, while coliform was detected on workbenches and floors. In conclusion, hygienic practices appear to have been adequately managed in school cafeterias despite COVID-19 driven changes. Nonetheless, continuous monitoring is recommended to ensure prompt response to changing environments.

• 대한치위생과학회지
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• 제4권2호
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• pp.1-7
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• 2021

Background: To identify the factors that affect the current status and satisfaction of people with disabilities at community oral care centers. Methods: A structured self-administered survey, including five questions on facility environment, five on usage procedure, four on medical skill, four on care cost, three on friendliness, and three on satisfaction, was administered to 218 residents of the G-disabled community care center. It comprised a Likert 5-point scale (strongly agree, 5 points; agree, 4 points; moderate, 3 points; disagree, 2 points; not at all, 1 point). The reliability of the measurement tool was 0.932 for Cronbach's α. Results: The evaluation of community oral care centers for the disabled showed that the environment was hygienic (4.42±0.73), reservation system was well maintained (4.18±0.95), and the dentist-in-charge was satisfied with the treatment (4.37±0.62). The participants agreed that the details were sufficiently explained (4.29±0.71). However, in terms of medical expenses, the score of "have fully heard the explanation of medical expenses and reductions" was 3.88±0.92. The factors affecting satisfaction were sex, final educational background in the facility environment, usage procedure, and medical skill. Conclusion: To increase the satisfaction of people with disabilities at community oral care centers, it is necessary to establish a facility environment and service according to the patients' need and increase the reduction or exemption benefits between different treatment cost categories. Oral health management policies for the disabled should be developed based on these factors, so that the oral care of vulnerable groups in blind spots can be maintained.

• 한국식품영양과학회지
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• 제40권7호
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• pp.1032-1042
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• 2011

본 연구는 군장병과 급양관을 대상으로 군 급식에 대한 이용실태, 인식 및 급식품질에 대한 중요도 및 수행도와 전반적인 만족도를 분석함으로써 향후 군 급식의 질 향상에 기초가 되는 자료를 제공하고자 하였다. 조사대상 군장병의 계급은 일병 32.6%, 상병 116명 29.3%, 병장 22.5%이었고, 연령은 20~25세 미만이 96.0%로 가장 많았으며 학력은 대부분(84.6%)이 대학 재학 중인 것으로 나타났다. 조사대상 급양관 중 남성이 92.3%, 여성은 1명 7.7%이었고 조사대상군 급식소는 직영으로 운영되고 있었으며, 조사대상 급양관은 영양사 자격증을 소유하고 있지 않았다. 식단계획은 지역 급식회의를 통해 메뉴선정이 되었고 군급식소의 배식방법은 부분적 자율배식을 실시하는 곳이 53.8%, 자율배식을 실시하는 곳이 38.5%이었으며, 메뉴작성 주기는 한 달에 1번작성하는 곳이 69.2%이었다. 조사대상 군장병의 식사시간은 10~20분 이내가 58.3%로 나타났고, 잔반이 많은 끼니는 아침(62.4%)이, 많이 남기는 잔반으로는 국(66.9%)과 반찬(20.2%)으로 나타났다. 급양관 대상 군 급식 운영에 대한 의견 조사 결과 급식 운영 시 어려운 사항으로 급식시설의 부족 및 노후화(28.6%)가 가장 높은 응답률이었고, 취사병수의 부족(14.3%), 숙달된 취사병 부족(10.7%) 등이 있었다. 취사병관련 개선사항에 대해 작업량에 적절한 취사병 수의 배치(46.2%)가 가장 높은 응답률이었고 작업 활동의 능률성향상(30.8%)과 취사병의 청결한 복장 및 외모(23.1%) 등이 있었다. 군장병의 급식의 질 향상을 위한 고려사항으로는 음식의 맛이 40.6%, 배식량 증가가 30.4%, 위생상태 개선 13.6% 등이 요구되는 것으로 나타났다. 군장병이 군 급식으로 선호하는 조리법은 볶음류(45.7%)와 튀김류(25.8%)였으며, 선호하는 식재료의 종류로는 육류(78.5%)가 가장 높은 응답률이었다. 식단에 대한 희망사항으로는 양식메뉴의 증가(25.7%), 한식메뉴의 증가(21.5%)로 나타나 식단메뉴의 다양화를 요구하는 것으로 나타났다. 군 급식의 39개 속성의 품질특성과 이를 구분한 5개 영역으로 군 급식에서 제공되는 급식의 품질특성에 대한 중요도와 수행도를 평가한 결과 속성과 영역 모두에서 중요도가 수행도보다 높게 평가되어 모든 품질에 대해 개선이 요구되는 것으로 나타났다. 중요도 점수가 가장 높은 속성은 위생영역의 음식위생(평균 4.59점)이었고, 음식영역의 '음식의 외관'이 평균 3.70점으로 가장 낮았다. 수행도 점수가 가장 높은 속성은 시설영역의 '식단의 사전 공지'(평균 4.09점)이었고, 메뉴영역의 '다양한 메뉴'가 평균 3.28점으로 가장 낮았다. 또한 '음식의 신선도', '음식의 간', '냉 난방의 적절성', '음식의 맛', '환기'로 중요하다고 판단되지만 잘 수행되지 않는 것으로 나타났다. 전반적인 만족도는 평균 3.43점(5점 만점)으로 보통 수준의 만족도 점수를 나타냈다. 따라서 음식의 맛 개선, 메뉴관리의 체계화, 위생관리 향상 등을 통한 군 급식의 질적 향상을 위해 전문적인 지식과 자격을 갖춘 영양관리직 군무원의 인력배치가 요구된다. 또한, 식단의 조리법을 다양화하고 음식의 맛 향상과 조리시간 단축을 위해 급식 시설부족 및 노후화의 개선이 요구되며, 중요하게 생각하지만 군대 급식소에 의해 잘 수행되지 않는 것으로 나타나고 전반적인 만족도에 영향을 미치는 '음식의 맛' 속성 질적 개선을 위해 식단의 질적 개선이 요구된다. 또한 군 급식비는 국방부 전체 예산의 4.3%로 타 예산 항목대비 감액 편성되고 있으나 군장병의 기호도를 반영한 영양적으로 균형 있는 급식제공을 위해서는 군장병의 급식 재원의 확대가 필요할 것으로 판단된다.

• 한국방사선학회논문지
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• 제8권7호
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• pp.467-477
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• 2014

본 연구의 목적은 교육용 치과 엑스선 발생장치를 설치 및 가동 중인 전국 치위생(학)과를 대상으로 방사선 작업종사자의 방사선 안전 관리 실태를 설문 조사하고 이를 체계적으로 추이 분석하여 교육용 치과 엑스선 발생장치의 안전한 이용 기반을 확립하기 위한 기초 자료로 제공하고자 하였다. 이를 위하여 교육용 치과 엑스선 발생장치의 방사선 안전 관리와 관계되는 원자력 관계 법령 및 국내 방사선 안전성 평가 및 규제 기술 개발 현황에 대한 용역 보고서를 참고하여 포괄적인 문헌 조사를 하였으며 이를 토대로 응답자의 일반적 특성, 방사선안전관리자의 위상, 방사선 안전관리 현황과 지식 및 의식 수준에 대한 설문지를 작성하였다. 본 연구는 국내 교육기관 내 방사선안전관리자와 교육용 치과 엑스선 발생장치를 운전 가능한 학사 및 실습 조교, 전임 교원 224명을 대상으로 설문 조사를 시행하여 설문 응답 누락 및 불충분한 응답을 제외한 95부를 회수하여 분석 자료로 이용하였다. 분석 방법은 일반적 특성 및 방사선안전관리자의 위상은 빈도와 백분율을 구하였으며 방사선 안전 관리 현황과 지식 및 의식 수준은 빈도 분석과 설문 문항별 연관성 분석을 위한 ${\chi}^2$ 검정(chi-square test)과 수준간 연관성을 구하기 위한 피어슨 상관분석(Pearson correlation analysis)을 하였다. 결과적으로 교육용 치과 엑스선 발생장치의 운영은 대부분 대학 이상의 사회적 교육 수준이 높고 치위생학을 전공한 20대에서 40대의 여성이 담당하고 있었으며 남성과 비교하여 방사선 안전 관리에 대한 의식 수준이 높게 나타났으며 통계적으로 유의한 선형적 관계를 보였다(${\chi}^2$ >5, 0.1${\chi}^2$ >5, 0.3${\chi}^2$ >5, 0.3