• Korean Journal of Poultry Science
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• v.27 no.2
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• pp.155-161
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• 2000

Unfortunately, there is no technique which is stable and repetitive to produce transgenic chicken, although various ways of gene transfer including PGC-and embryonic cell-mediated gene transfer, DNA microinjection, virus inoculation and sperm cells have been employed. The aims of this study were 세 develop and establish such a stable, repetitive and efficient way of gene transfer giving a faithful gene expression during development after the reconstruction of embryo in an UV-irradiated egg. A dual reporter plasmid (pJJ9), a fusion gene containing lacZ and GFP driven by a CMV promoter was used to exploit either merits of both reporting markers. lacZ with strong signal or GFP with vital marking. Electroporated embryonic blastodermal cells (EBCs) in the presence of the pJJ9 DNA faithfully showed 377 bp PCR product and lacZ or GFP expressions in the identical cells in vitro of in vivo. Furthermore, analyses of expression pattern of the foreign DNA demonstrated that microinjected EBCs cells into the UV-irradiated recipient egg should participate in normal developmental process, for example, proliferation and differentiation into various tissues. Thirty percentages of the manipulated eggs showed lacZ expression in their tissues. These results together with the specific procedures used in this study should facilitate avian transgenesis.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.6
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• pp.831-837
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• 2013

An experiment was conducted to investigate the effects of dietary supplementation of Rhodobacter capsulatus KCTC-2583 on egg-yolk and serum cholesterol, egg production and quality parameters during the late laying periods in hens. A total of 160 Hy-Line Brown layers (54 wk-old) were randomly allotted to 4 treatment groups on the basis of laying performance. Each treatment had 4 replicates with 10 birds each (40 birds per treatment). Two hens were confined individually with cage size $35{\times}35{\times}40$ cm and each 10 birds (5 cages) shared a common feed trough between them forming one experimental unit. Dietary treatments were; basal diet supplemented with 0 (control), 0.05, 0.10 and 0.15% R. capsulatus KCTC-2583. Experimental diets were fed in meal form for 56 d. Dietary supplementation of increasing levels of R. capsulatus KCTC-2583 reduced (linear, p<0.05) egg-yolk cholesterol and triglycerides (d 28, 42 and 56) concentrations. Also, serum cholesterol and triglycerides (d 21, 42 and 56) concentrations were linearly reduced (p<0.05) with increasing dietary R. capsulatus KCTC-2583. Laying hens fed a diet supplemented with increasing levels of R. capsulatus KCTC-2583 had increased (linear; p<0.05) overall egg production, egg weight, egg mass and feed efficiency. However, dietary treatments had no effect (linear or quadratic; p>0.05) on feed intake of laying hens. At d 28 and 56, breaking strength and yolk colour of eggs were linearly improved (p<0.05) in laying hens fed dietary increasing levels of R. capsulatus KCTC-2583. Dietary treatment had no effects (linear or quadratic; p>0.05) on albumin height, shell thickness and shell weight at any period of experiment. These results indicate that dietary supplementation of R. capsulatus KCTC-2583 has the potential to improve the laying hen performance and lead to the development of low cholesterol eggs during late laying period in Hy-Line Brown hens.

• Korean Journal of Poultry Science
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• v.14 no.1
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• pp.1-8
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• 1987

Three experiments were conducted to investigate effects of dietary energy and protein levels on performance of laying hens. A total of 360 hens each of 352-day old Manina Brown (Exp.1), 280-day old Brown Warren (Exp.2) or 3174ay old Brown Warren (Exp.3) was divided into 20 pens of 18 birds. Each pen was assigned to one of the four dietary treatments with 5 replications according to a 2${\times}$2 factorial design, consisting of all possible cominations of two levels of metabolizable energy (2,500 and 2,800 kcal/kg of diet) and two levels of crude Protein (13 and 16%). All hens were housed two birds per cage. Exp.1 and 2 were conducted for four weeks and hens were fed experimental diets ad libitum, and Exp.3 lasted two weeks and feed consumption was restricted to 130g/hen/day. In Exp.1 and 2, increasing either energy or protein level in the diet numberically improved egg production. However, in Exp. 3, where feed consumption was restricted, egg Production was affected significantly 〈0.05) by the energy levies and numerically by the protein levels. Neither protein nor energy level influenced egg weight in Exp. 1 and 2, but in Exp. 3 the higher level of energy improved egg weight numerically, In all three experiments increasing either protein or energy level increased egg mass. Higher levels of either protein and energy tended to decrease feed consumption and improve feed conversion rate numerically. It should also be noted that the higher level of energy improved egg mass produced per unit intake of protein and the higher level of Protein improved egg mass produced per unit intake of energy.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.1
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• pp.32-39
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• 2000

Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of glucose-xylose mixture was carried out by the yeast Saccharomyces cerevisiae in the presence of a compatible xylose isomerase. The enzyme converted xylose to xylulose and S. cerevisiae fermented xylulose, along with glucose, to ethanol at pH 5.0 and $30^{\circ}C$. This compatible xylose isomerase from Candida boidinii, having an optimum pH and temperature range of 4.5-5.0 and $30-35^{\circ}C$ respectively, was partially purified and immobilized on an inexpensive, inert and easily available support, hen egg shell. An immobilized xylose isomerase loading of 4.5 IU/(g initial xylose) was optimum for SIF of xylose as well as SICF of glucose-xylose mixture to ethanol by S. cerevisiae. The SICF of 30 g/L glucose and 70 g xylose/L gave an ethanol concentration of 22.3 g/L with yield of 0.36 g/(g sugar consumed) and xylose conversion efficiency of $42.8\%$.

• Journal of Animal Environmental Science
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• v.12 no.1
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• pp.21-28
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• 2006

This study was carried out to measure and analyze the characteristics of noise and vibration, and to analyze their effects on the productivity of layers, mechanical troubles, and abnormal wear-out failure of facilities and equipment of the layer house. The measurements of noise and vibration were taken at 13 layer farms nationwide for the operations of feed supplier system, feed distribution system, automatic egg collection system, ventilation system, blot conveyer for layer feces, and fur the case of with and without their operation by a sound level meter and a vibration measuring system in the layer house equipped with upright multi-tier cages. Measurement results showed that normal times were noise(N) 82 dB and vibration(V) 0.2072 cm/s, feed supplier system were 90 dB(N) and 2.8560 cm/s(V), feed distribution system were 90 dB(N) and 2.0222 cm/s(V), automatic egg collection system were 87 dB(N) and 0.1865 cm/s(V), ventilation system 88 dB(N) and 2.5364 cm/s(V), belt conveyer fur layer feces were 88 dB(N) and 0.2387 cm/s(V), and then maximum values of noise and vibration were 90 dB and 2.8560 cm/s, respectively, when feeding systems(feed supplying system and feed distribution system) were operated. Based on these results, an experiment is being conducted to find out the effect of noise and vibration on the productivity of layers in the layer house equipped with upright multi-tier cages.

• Korean Journal of Poultry Science
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• v.32 no.2
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• pp.143-147
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• 2005

This study was conducted to evaluate the feeding value of the Flammulina veluipes media by-product(FMB) in laying hens(Hy-Line Brown). A total of three hundreds sixty were fed corn-soy based experimental diets containing 0(control), 5.0(T1), and $10\%(T2)$ FMB for 12 weeks. There were no significant differences among the treatments in egg production, egg weight, egg mass, feed conversion and viability during the experimental period. Feed intake was significantly(P<0.05) lowered in control(120.5g) than 72(123.9g). There were no significant differences among the treatments eggshell breaking strength, thickness and haugh unit, whereas the yolk color of T1 and T2 were significantly love. than control(p<0.05). In conclusion, the FMB can be used as resource of feed in laying hen feed at $5.0\%$ level without effect on performance and egg qualify. However, dietary pigments must be added when the FMB was used as a feed ingredients more than $10\%$ in diets.

• Korean Journal of Poultry Science
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• v.35 no.1
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• pp.1-7
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• 2008

Three dietary herbs, Coptis japonica Makino (48.5%), Lonicera japonica Thunb (48.5%), and Morus alba L. (3%) were mixed and used as additives in hen's feed. One hundred-eight, 28-wk-old Lohmann Brown hens were assigned randomly with three diet treatments (0, 0.3, and 1% of herb mix). Hens were fed for 6 wks to investigate the effect of herb mix on the physicochemical quality of eggs during storage at $4^{\circ}C$ for 0, 3, 7, and 14 days. Proximate composition including the content of water, protein, fat, and ash had no difference among 3 diet treatments. Objective color of cooked whole egg from hens fed herb mix did not show any effect compared with control except for the color $a^*$- and $b^*$-values at day 3. However, color $a^*$- and $b^*$-values increased during storage regardless of treatment. From the texture analysis, eggs from hens fed with the herb mix were not different compared with control remove for springness at day 14. However, it was observed that springness and gumminess were decreased as storage period increased. Therefore, a dietary supplementation of herb mix to hen may not affect on proximate composition, color and texture of eggs.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.2
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• pp.313-322
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• 2020

Objective: An experiment was conducted to investigate the response of laying hens fed corn distiller's dried grains with solubles (DDGS) that are naturally contaminated with deoxynivalenol (DON). Methods: One hundred and sixty 52-week-old Lohmann Brown Lite hens were randomly allotted to five dietary treatments with 8 replicates per treatment. The dietary treatments were formulated to provide a range of corn DDGS contaminated with DON from 0% to 20% (i.e., 5% scale of increment). All laying hens were subjected to the same management practices in a controlled environment. Body weight, feed intake and egg production were measured biweekly for the entire 8-week experiment. The egg quality was measured biweekly for 8 weeks. On weeks 4 and 8, visceral organ weights, blood metabolites, intestinal morphology, and blood cytokine concentrations were measured. Results: The inclusion of corn DDGS contaminated with DON in the diet did not alter (p>0.05) the body weight, feed intake, hen-day egg production, egg mass and feed efficiency of the laying hens. No difference was found (p>0.05) in the egg quality of hens that were fed the dietary treatments. Furthermore, hens that were fed a diet containing corn DDGS contaminated with DON showed no change (p>0.05) in the visceral organ weights, the blood metabolites, and the cytokine concentrations. The crypt depth increased (p<0.05) as the amount of corn DDGS contaminated with DON increased. Proportionately, the villus height to crypt depth ratio of the laying hens decreased (p<0.05) with the increasing level of corn DDGS contaminated with DON in the diet. Conclusion: The inclusion of corn DDGS contaminated with DON up to 20% in layer diets did not cause changes in egg production performance and egg quality, which indicates that DON is less toxic at the concentration of 1.00 mg DON/kg.

• Korean Journal of Poultry Science
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• v.7 no.1
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• pp.1-11
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• 1980

About 6 to 8% of the eggs annually produced are broken or cracked between the hen and the consumer's carton. The majority of this breakage is due to interrelationships between egg shell quality and the many biological, environmental and managerial factors which have been individually identified as affecting breakage. Some of the factors associated with shell damage discussed in this review include age of hens, temperature ana humidity of the laying house, design of the cage systems and of the cage floors, type of material used to manufacture the cages frequency of daily egg collection in the laying house and, Probably most important, the frequency and quality of handling equipment maintenance. Age and genetic constitution of the hens, environmental temperature, and design and quality of equipment maintenance are the major factors that influence shell breakage. There is a curvilinear relationship between shell quality and shell breakage which explains why small changes in shell quality may be associated with large changes in the incidence of breakage. Published data indicate the incidence of breakage may range from as low as 1 to 2% to 35% or more for other egg producers.

• Korean Journal of Poultry Science
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• v.33 no.3
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• pp.189-194
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• 2006

This study was conducted to investigate the effects of natural mineral complex supplementation on egg production and characteristic in laying hens. A total of two hundred forty laying hens were randomly allocated into four treatments with ten replications for six weeks. Dietary treatments included 1) CON (control, basal diet) 2) M0.5 (basal diet + 3% chitosan + 0.5% natural mineral complex), 3) M1.0 (basal diet + 3% chitosan + 1.0% natural mineral complek) and 4) M1.5 (basal diet + 3% chitosan + 1.5% natural mineral complex). In the egg production, the M1.5 treatment was significantly higher than other treatments(P<0.05). However, egg weight was significantly higher in M0.5 treatment than CON and M1.0 treatments(P<0.05). Egg shell breaking strength was higher in M1.5 treatment than M0.5 treatment. Egg shell thickness was the highest in M1.5 treatment compared to other treatments(P<0.05). The hens 134 M0.5 diet were improved egg yolk color compared to those fed other diets(P<0.05). The Haugh unit, CON and M1.0 treatments showed significantly different results compared to those of M1.5 treatment(P<0.05). The M1.5 treatment had higher Ca and Fe concentration in blood and higher K concentration in yolk than CON(P<0.05). In conclusion, supplementation of natural mineral complex in laying hen diets influenced on egg production, egg shell qualify and mineral concentration in blood and yolk.