Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
권호 표지

Recolonization of Transfected Blastodermal Cells in Developing Embryos after Transferring into UV-irradiated Fertilized Hen′s Egg

UV-조사 수정란 내로 이식한 유전자 변화 배반엽 세포의 재구성

  • Lee, K.S. (Division of Life Sciences and Graduate School of Biotechnology, Korea University) ;
  • Lee, H. (Division of Life Sciences and Graduate School of Biotechnology, Korea University) ;
  • Kim, K.D. (Division of Life Sciences and Graduate School of Biotechnology, Korea University) ;
  • Park, S.S. (Division of Life Sciences and Graduate School of Biotechnology, Korea University) ;
  • Lee, S.H. (Division of Life Sciences and Graduate School of Biotechnology, Korea University)
  • 이기석 (고려대학교 생명과학부, 생명공학원) ;
  • 이황 (고려대학교 생명과학부, 생명공학원) ;
  • 김기동 (고려대학교 생명과학부, 생명공학원) ;
  • 박성수 (고려대학교 생명과학부, 생명공학원) ;
  • 이상호 (고려대학교 생명과학부, 생명공학원)
  • Published : 2000.08.01
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Abstract

Unfortunately, there is no technique which is stable and repetitive to produce transgenic chicken, although various ways of gene transfer including PGC-and embryonic cell-mediated gene transfer, DNA microinjection, virus inoculation and sperm cells have been employed. The aims of this study were 세 develop and establish such a stable, repetitive and efficient way of gene transfer giving a faithful gene expression during development after the reconstruction of embryo in an UV-irradiated egg. A dual reporter plasmid (pJJ9), a fusion gene containing lacZ and GFP driven by a CMV promoter was used to exploit either merits of both reporting markers. lacZ with strong signal or GFP with vital marking. Electroporated embryonic blastodermal cells (EBCs) in the presence of the pJJ9 DNA faithfully showed 377 bp PCR product and lacZ or GFP expressions in the identical cells in vitro of in vivo. Furthermore, analyses of expression pattern of the foreign DNA demonstrated that microinjected EBCs cells into the UV-irradiated recipient egg should participate in normal developmental process, for example, proliferation and differentiation into various tissues. Thirty percentages of the manipulated eggs showed lacZ expression in their tissues. These results together with the specific procedures used in this study should facilitate avian transgenesis.

Keywords

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