• Title/Summary/Keyword: h-stable

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Isolation of Aspergillus niger K-25 Prroducing Acid-stable ${\alpha}-amylase$ (내산성(耐酸性) 아밀라제를 생산하는 Aspergillus niger 균주의 분리)

  • Cho, Myung-Hwan
    • The Korean Journal of Mycology
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    • v.17 no.3
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    • pp.149-153
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    • 1989
  • One strain of Aspergillus niger K-25 producing an acid-stable ${\alpha}-amylase$ was isolated from the soil. The optimum culture conditions were investigated. The production of the acid-stable ${\alpha}-amylase$ was enhanced when the strain was incubated in a medium containing soluble starch 3.5%, peptone 2%, $KH_2PO_4$ 0.5%, $MaSO_4{\cdot}7H_2O$ 0.25% and $FeCI_3$ 1.0% at pH 3 for 7 days. However, higher activity of acid-stable ${\alpha}-amylase$ was demonstrated on wheat bran culture. Amylase production was doubled when A. niger K-25 was incubated on the wheat bran supplemented with fumaric acid buffer (pH 3).

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Intramolecular Hydrogen Bonding in 2-Fluorocyclopropanemethanol and 2-Chlorocyclopropanemethanol as Studied by ab Initio Calculation (2-Fluorocyclopropanemethanol과 2-Chlorocyclopropanemethanol의 분자 내 수소결합 가능성에 대한 이론연구)

  • Kwon, Min-Kyeong;Sung, Eun-Mo
    • Journal of the Korean Chemical Society
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    • v.54 no.3
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    • pp.275-282
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    • 2010
  • 2-Fluorocyclopropanemethanol and 2-chlorocyclopropanemethanol have been studied with MP2 and B3LYP methods with 6-311++G(d,p) basis set. The optimized structures show several stable conformers. The most stable conformer show the possibility of intramolecular hydrogen bonding, but the distance between $H{\cdots}F$, or $H{\cdots}Cl$ is longer than van der Waals radii and it may not be strong covalent bonding. Rather the second stable conformer has optimum structure for intramolecular hydrogen bonding but the energy of the conformer is 5 ~ 7 kJ higher than the most stable conformer. When the methanol group and the F or Cl atom have opposite direction, the conformers are less stable than the most stable conformer.

SOME CURIOSITIES OF THE ALGEBRA OF BOUNDED DIRICHLET SERIES

  • Mortini, Raymond;Sasane, Amol
    • Bulletin of the Korean Mathematical Society
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    • v.53 no.1
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    • pp.153-161
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    • 2016
  • It is shown that the algebra $\mathfrak{H}^{\infty}$ of bounded Dirichlet series is not a coherent ring, and has infinite Bass stable rank. As corollaries of the latter result, it is derived that $\mathfrak{H}^{\infty}$ has infinite topological stable rank and infinite Krull dimension.

H$\"{O}$LDER CONTINUITY OF H-SSSI S$\alpha$S PROCESSES

  • Kim, Joo-Mok
    • Communications of the Korean Mathematical Society
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    • v.15 no.1
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    • pp.123-131
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    • 2000
  • Let {X(t) : t $\geq$B 0} be a Symmetric $\alpha$ Stable and H-Self-similar process with stationary increments. We examine a.s. Holder unboundedness of S$\alpha$S H-sssi Chentsov processes and H-sssi Chentsov fields for order ${\gamma}$>H. Finally, we prove a.s. Holder continuity of S$\alpha$S H-sssi processes with ergodic seating transformations for the case of H>1/$\alpha$.

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Production of Bleach-Stable and Halo-Tolerant Alkaline Protease by an Alkalophilic Bacillus pumilus JB05 Isolated from Cement Industry Effluents

  • Johnvesly, B.;Naik, Gajanan R.
    • Journal of Microbiology and Biotechnology
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    • v.11 no.4
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    • pp.558-563
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    • 2001
  • A new alkalophilic strain of Bacillus pumilus JB¬05 producing bleach-stable and halo-tolerant alkaline protease was isolated from cement industry effluents in Karnataka, India. The effects of carbon and nitrogen sources on protease production by this alkalophilic strain were observed after a 30-h incubation. A high level of alkaline protease activity was obtained in the presence of starch as the carbon and peptone as the nitrogen sources. The partially purified enzyme showed an optimum temperature and pH activity at $58^{\circ}C$ and 10.5, respectively. The enzyme was completely inhibited by PMSF (95.0%) indicating it as a serine protease. It is bleach-stable as it retained 35% original activity in the presence of 10% (v/v) hydrogen peroxide at $30^{\circ}$C after 2 h and is halo-tolerant as it retained 70% original activity in the presence of 2.5 M sodium chloride at $30^{\circ}C$ after 2 h incubation.

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The Effect of Temperature on the Stable Region of Magnesium Ion in Aqueous System (수중 마그네슘이온의 안정영역 변화에 대한 온도효과)

  • Kim, Hee-Jin;Kim, Dong-Su
    • Journal of Korean Society on Water Environment
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    • v.27 no.4
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    • pp.438-444
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    • 2011
  • Magnesium is one of the abundant natural resources in the earth crust and seawater, which is directly related to various organisms activities interconnecting with water-rock system. In aqueous system, magnesium is known to predominantly exist in the form of $Mg^{2+}$ ion which is verified in its $E_h-pH$ diagram. When it is at equilibrium in aqueous system, temperature takes an essential role to complete equilibrium states. This study represents the change of the stable region of magnesium ion according to temperature, and how the consequences would affect aquatic organisms. It was revealed that there is a noticeable tendency shrinking the stable region of magnesium ion in a diagram as temperature increases, and as a result, aquatic bio-species presumably have difficulties to absorb the nutrient. Also, it was considered that the water system would be acidified by decreasing alkalinity.

Variation of Optimum Operational pH in Partial Nitritation (암모니아 폐수의 부분아질산화에서 최적 운전 pH의 변동)

  • Bae, Wookeun;Khan, Hammad
    • Journal of Korean Society of Environmental Engineers
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    • v.38 no.5
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    • pp.228-235
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    • 2016
  • Nitrite accumulation is essential for constructing an anammox process. As the pH in the reactor exerts a complicated and strong influence on the reaction rate, we investigated its effects upon treatment of an ammonic wastewater (2,000 mgN/L) through modeling and experiment. The modeling results indicated that the reaction stability is strongly affected by pH, which results in a severe reduction of the 'stable region' of operation under alkaline environments. On a coordinate of the total ammonia nitrogen (TAN) concentration vs. pH, the maximal stable reaction rates and the maximal nitrite accumulation potentials could be found on the 'stability ridge' that separates the stable region from the unstable region. We achieved a stable and high ammonia oxidation rate (${\sim}6kgN/m^3-d$) with a nitrite accumulation ratio of ~99% when operated near the 'stability ridge'. The optimum pH that can be observed in experiments varies with the TAN concentrations utilized, although the intrinsic optimum pH is fixed. The direction of change is that the optimum operational pH falls as the TAN concentration increases, which is in excellent accordance with the observations in the literature. The optimum operational pH for 95% nitritation was predicted to be ~8.0, whereas it was ~7.2 for 55% partial nitritation to produce an anammox feed in our experimental conditions.

Regulation of Heat-Stable Enterotoxin Production in Escherichia coli -1. Effeets of Phosphate, Ammonia, Glucose, and Glucose Metabolites on the Heat-Stable Toxin Production by Enterotoxigenic Escherichia coli- (대장균의 내열성장독소 생산조절기전 -I. 장독성대장균의 내열성장독소생산에 인산염, 암모니아, 포도당 및 포도당 대사산물이 미치는 영향-)

  • Kim, Ik-Sang;Hong, Tae-Yee;Lee, Woo-Kon;Chang, Woo-Hyun
    • The Journal of the Korean Society for Microbiology
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    • v.20 no.1
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    • pp.55-63
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    • 1985
  • Phosphate, ammonia, glucosamine, glucose, pyruvate, succinate, fumarate, malate and acetate were examined for their ability to control the heat-stable enterotoxin (ST) production in succinate salts medium or in M9 medium. The results obtained were summerized as follows. 1. When the initial phosphate concentration was adjusted to 1.0mM, ST production was decreased to 80u/ml or less. But when the initial phosphate concentration was adjusted to 64mM or 100mM, enterotoxin production was 320u/ml. 2. When the initial ammonia concentration in the medium was adjusted to 1.0mM, no ST production and cell growth were observed. But when ammonia concentration was adjusted to 10mM, 19mM, 38mM or 76mM, enterotoxin production was 320u/ml. 3. Among carbon sources, glucosamine, glucose, pyruvate, succinate, fumarate, malate and acetate, acetate supported the highest specific production (928 unit/O.D.) of heat-stable enterotoxin. From this results, we could assume that heat-stable enterotoxin production is controlled by stringent control mechanism. 4. When the pH of the succinate salts medium was kept between 6.2 to 6.5, no heat-stable enterotoxin production was observed, but when the pH of the medium was kept between pH 6.2 to 6.5, 267 unit/O.D. of heat-stable enterotoxin was produced. 5. Glucose inhibited the heat-stable enterotoxin production and the mechanism was assumed due to its capacity to lower the pH of the medium during catabolysis and its high metabolic energy.

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Assessment of bovine blood sample stability for complete blood count and blood gases and electrolytes analysis during storage

  • Espiritu, Hector M.;Faruk, Shohel Al;Lee, Gyeong-jae;Lopez, Bryan Irvine M.;Lee, Sang-suk;Cho, Yong-il
    • Korean Journal of Veterinary Service
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    • v.42 no.4
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    • pp.265-274
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    • 2019
  • Delayed arrival of blood samples from the field and a large number of samples delivered often causes delay in sample analysis leading to inaccurate measurements. Therefore, this study aimed to assess whether prolonged storage in refrigerator could influence the stability of cattle blood samples and to establish an optimal time limit for complete blood count (CBC) parameters and blood gas and electrolyte (BGE) parameters analyses. Samples collected from healthy cows were tested immediately for CBC and BGE using automated hematology, blood gas and electrolyte analyzers. Samples were kept in refrigerator at 4℃ and analyzed after 6 h, 12 h, 24 h, 48 h, 72 h, 120 h, and 192 h of storage. Mean differences between observations were assessed at 5% significance level using ANOVA and Duncan's multiple range test. Total CBC parameters and the platelet profile remained stable for 192 h, except for MCHC. Among leukocyte-related counts, NEU and EOS remained stable for 192 hours. WBC and LYM, and MONO values produced inconsistent measurements which recovered its initial measurement after 12 h and 24 h of storage, respectively, then remained stable until 120 h. Among the blood gas indices, PCO2, PO2, tCO2, and BE showed declining and significant changes over time, but pH, tHb, and SO2 remained stable for 192 h. Electrolyte status in the blood showed that ions are unstable and tend to change in as early as 6 h of storage. This study established that cattle blood specimens for CBC analysis can be stored for 120 h at 4℃, but specimens for BGE analyses must be tested within 6 to 24 h.