• Title/Summary/Keyword: growth conditions

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Activation of Multiple Transcriptional Regulators by Growth Restriction in Pseudomonas aeruginosa

  • Yeom, Doo Hwan;Im, Su-Jin;Kim, Soo-Kyoung;Lee, Joon-Hee
    • Molecules and Cells
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    • v.37 no.6
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    • pp.480-486
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    • 2014
  • Growth restriction by antibiotics is a common feature that pathogenic bacteria must overcome for survival. The struggle of bacteria to escape from growth restriction eventually results in development of antibiotic-resistance through the expression of a set of genes. Here we found that some physiologically important transcriptional regulators of Pseudomonas aeruginosa including QscR, a quorum sensing (QS) receptor, SoxR, a superoxide sensor-regulator, and AntR, a regulator of anthranilate-related secondary metabolism, are activated by various growth-restricted conditions. We generated the growth-restricted conditions by various methods, such as overexpression of PA2537 and treatment with antibiotics or disinfectants. The overexpression of PA2537, encoding an acyltransferase homologue, tightly restricted the growth of P. aeruginosa and significantly activated QscR during the growth restriction. Similarly, treatments with gentamycin, tetracycline, and ethanol also activated QscR near their minimal inhibitory concentrations (MICs). Some non-QS regulators, such as AntR and SoxR, were also activated near the MICs in the same conditions. However, LasR and PqsR, other QS receptors of P. aeruginosa, were not activated, suggesting that only a specific set of transcriptional regulators is activated by growth restriction. Since paraquat, a superoxide generator, significantly activated QscR and AntR, we suggest that the oxidative stress generated by growth restriction may be partly involved in this phenomenon.

Optimization of Growth Environments Based on Meteorological and Environmental Sensor Data (기상 및 환경 센서 데이터 기반 생육 환경 최적화 연구)

  • Sook Lye Jeon;Jinheung Lee;Sung Eok Kim;Jeonghwan Park
    • Journal of Sensor Science and Technology
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    • v.33 no.4
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    • pp.230-236
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    • 2024
  • This study aimed to analyze the environmental factors affecting tomato growth by examining the correlation between weather and growth environment sensor data from P Smart Farm located in Gwangseok-myeon, Nonsan-si, Chungcheongnam-do. Key environmental variables such as the temperature, humidity, sunlight hours, solar radiation, and daily light integral (DLI) significantly affect tomato growth. The optimal temperature and DLI conditions play crucial roles in enhancing tomato growth and the photosynthetic efficiency. In this study, we developed a model to correct and predict the time-series variations in internal environmental sensor data using external weather sensor data. A linear regression analysis model was employed to estimate the external temperature variations and internal DLI values of P Smart Farm. Then, regression equations were derived based on these data. The analysis verified that the estimated variations in external temperature and internal DLI are explained effectively by the regression models. In this research, we analyzed and monitored smart-farm growth environment data based on weather sensor data. Thereby, we obtained an optimized model for the temperature and light conditions crucial for tomato growth. Additionally, the study emphasizes the importance of sensor-based data analysis in dynamically adjusting the tomato growth environment according to the variations in weather and growth conditions. The observations of this study indicate that analytical solutions using public weather data can provide data-driven operational experiences and productivity improvements for small- and medium-sized facility farms that cannot afford expensive sensors.

Effects of thermal boundary conditions and microgravity environments on physical vapor transport of $Hg_2Cl_2-Xe$ system

  • Kim, Geug-Tae;Kwon, Moo-Hyun;Lee, Kyong-Hwan
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.19 no.4
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    • pp.172-183
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    • 2009
  • For the effects of the nonlinear temperature profiles and reduced-gravity conditions we conduct a two-dimensional numerical modeling and simulations on the physical vapor transport processes of $Hg_2Cl_2-Xe$ system in the horizontal orientation position. Our results reveal that: (1) A decrease in aspect ratio from 5 to 2 leads to an increasingly nonuniform interfacial distribution and enhances the growth rate by one-order magnitude for normal gravity and linear wall temperature conditions. (2) Increasing the molecular weight of component B, Xenon results in a reduction in the effect of solutal convection. (3) The effect of aspect ratio affects the interfacial growth rates significantly under normal gravity condition rather than under reduced gravitational environments. (4) The transition from the convection-dominated regime to the diffusion-dominated regime ranges arises near at 0.1g$_0$ for operation conditions under consideration in this study.

Growth Conditions of Natural Monument Old Big Trees in Gyeongsangnamdo, Korea (경상남도 천연기념물 노거수의 생육환경 연구)

  • Kim, Hyo-Jeong
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.14 no.5
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    • pp.103-112
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    • 2011
  • Old big tree transcends the simple meaning of trees as they are the natural monuments that embody the people's history and culture of this land. The Cultural Heritage Administration of Korea(CHA) defines and protects old big tree based not only on the size of the tree but also on its definitive cultural and natural factors such as value, implications, and originality. This research aims to identify and analyze the growth conditions, soil conditions and location character of 20 old big tree in Gyeongsangnamdo korea. The research examined the soundness of the arboreal form, the degree of damage on the bark, as well as the quantity of leafs levels to evaluate the overall condition of growth and development. Also, 9 elements such as soil texture, nitrogen and organic matter content, soil pH, phosphoric acid and EC were further analyzed The research analyzed in correlation of Growth condition and soil. Tree health related positivity that total nitrogen and organic matter. The result which analyzes location character, With natural monument old big trees raising a hand the area where is contiguous appeared with the fact that the farming village style where the rice field and the arable land of field etc. This research aimed at generating some foundational reference data for the analysis of the habitation and management conditions of natural monument old big tree within the Gyeongsangnamdo korea.

A Study on the Growth of Savory According to the Composition of the Soil Inside a Green House (온실을 중심으로 토양 성분에 따른 세이보리의 생육현황에 대하여)

  • Shin, Gyung soon;Cho, Tae-Dong
    • Journal of Environmental Science International
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    • v.30 no.1
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    • pp.87-96
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    • 2021
  • Herb has been categorized as a special plants from the beginning of human history and used in different medical systems in different cultures. This research has classified soil into 6 kinds that have diverse elements to see to which various kinds of savory(satureja hortensis) adapt well, experimenting from sowing to flowering for around 13 weeks, and also divided indoor conditions to get the result below. In conclusion, growth status of savory depending on the kinds of soil suggest that in indoor conditions the savory if planted in ⑤ bed soil compounded with saprolite and poultry manure grew better than any other condition. On the other hand, the growth status was bad in ① masato, ② clay, ④ bed soil mixed with saprolite, and ⑥ bed soil mixed with clay conditions. Though you can see the immediate effect of soil on the growth of savory, I'd like to reveal the details of how elements of savory operate in which kind of soil and outdoor conditions the goal of this research, in the next research.

Rice (Oryza sativa L.) Growth Promotion by Various Plant Extracts Produced Using Different Extraction Methods

  • Ei Ei;Hyun Hwa Park;Yong In Kuk
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.53-53
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    • 2022
  • Modem agricultural production needs to provide sustainable management practices that are eco-friendly and low cost. Plant extracts are a cost-effective and environmentally friendly alternative to synthetic plant growth regulators. This study was therefore carried out to investigate the effects of various plant extracts produced using different extraction methods on the vegetative growth of rice under laboratory and greenhouse conditions. For this study, seventeen plant extracts were made from plant species such as leaves of M. arvense, C. asiatica, M. oleifera, V. radiata, V. unguiculate, P. guajava, A. vera, and A. tuberosum, aboveground plant parts of C. rotundus, M. sativa, and P. frutescens, roots of R. undulatum, tubers of A. sativum, leaves and stems of G. max (cv. Taegwang) as well as rice straw and hulls (cv. Hopyeong). As a test crop, we applied these extracts to rice plants. For the purpose of making our extracts, some plant materials and species were collected in fields and others were purchased from Chonnam Hanyaknonghyup Cooperation (South Korea). Leaves, roots, and aboveground plant parts of plant species were dried, ground, extracted (water, boiling water and ethanol) and fermented. Rice growth promotion effects were determined using plant extracts at 0, 0.05, 0.1, 0.5, and 1% concentrations under petri dish conditions. Seven selected plant extracts were applied to rice seeds with soil drench application or seedling at 3-4 leaf stages with soil and foliar applications under greenhouse conditions. For comparison with extracts, we used urea at 0.6%. Of the 17 water extracts used in this study, 10 extracts reduced rice growth, but the other 7 extracts (P. guajava, A. vera, A. tuberosum, M. sativa, A. sativum, and G. max) increased growth by 40-60% on compared to the control in Petri dish bioassay. Thus, these 7 extracts were selected for further study. Under greenhouse conditions, rice growth also increased by 20-40% when the same 7 extracts were applied to rice seeds using soil drench application. Furthermore, at the 3-4 leaf stage rice growth also increased 30-80% or 30-60% when the same 7 extracts were applied using soil and foliar applications. Overall, the 7 extracts produced higher rates of growth promotion when soil drench application was used than when foliar application was used. In the case of boiling water and ethanol extracts, rice growth increased only 20% in response to both soil drench and foliar application of the same 7 extracts. Rice growth promotion was greater when extracts were produced using water extraction method than boiling water and ethanol extraction methods. Most notably, the 7 water extracts used in this study produced higher rates of growth promotion than urea at 0.6% which is typically used for crop growth promotion. Overall, the 7 water extracts when applied using soil drenching method can be used as effective growth promotors of rice in organic agriculture.

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Morphological Characterization and Culture Conditions of A White Mutant of Ganoderma Iucidum (영지버섯 백색 변이주의 형태적 특성 및 최적 배양조건)

  • 조수묵;서건식;유승헌;유익동;신관철
    • Microbiology and Biotechnology Letters
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    • v.21 no.6
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    • pp.520-526
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    • 1993
  • A morphologically different form of Ganoderma lucidum was isolated from a cultivator's farm, and its optimum growth conditions were determined. A major difference in their morphology was color of fruit bodies. Fruit bodies of the mutant were white wherase those of normal Ganoderma lucidum were red. Spores of the mutant were global and mycelia were thin. Mycelial growth of this white mutant was favorable on potato sucrose agar medium, and optimum pH of the medium was 5.5.

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Growth and fatty Acid Composition with Growth Conditions for Spirulina platensis platensis (배양 조건에 따른 Spirulina platensis의 성장 및 지방산 조성)

  • Joo Dong-Sik;CHO Man-Gi;Buchholz Rainer;LEE Eung-H
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.3
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    • pp.409-416
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    • 1998
  • Owing to their high growth rate, marine microalgae such as Chlorella, Spirulina, Porphpidium and Dunaliella have been believed to be potentially useful for the production of foods, drugs and energy from light, $CO_2$ and minerals. In this study, we investigated the relationship between the growth and fatty acid composition in the blue green alga Spirulina platensis when the temperature and light intensity of culture conditions were changed. The optimal growth conditions for Spirulina platensis from the biomass and lipid contents were $30^{\circ}C$ on 6391 $\mu$E/$m^3$/sec and $35^{\circ}C$ on 4235 $\mu$E/$m^3$/sec. The difference of lipid contents between exponential phase and stationary phase were very large according to growth conditions. According to growth conditions the fatty acid compositions of Spirulina platensis differed, but regardless of growth conditions the main fatty acids were C16 : 0 and C20 : 0 in saturated fatty acid, C16 : 1, C18 : 1 and C 18 : 2 in unsaturated fatty acid.

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The Effects of Temperature and Nutritional Conditions on Mycelium Growth of Two Oyster Mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus)

  • Hoa, Ha Thi;Wang, Chun-Li
    • Mycobiology
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    • v.43 no.1
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    • pp.14-23
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    • 2015
  • The influences of temperature and nutritional conditions on the mycelium growth of oyster mushroom Pleurotus ostreatus (PO) and Pleurotus cystidiosus (PC) were investigated in laboratory experiment during the summer season of 2014. The results of the experiment indicated that potato dextrose agar (PDA) and yam dextrose agar (YDA) were the most suitable media for the mycelium growth of oyster mushroom PO while four media (PDA, YDA, sweet potato dextrose agar, and malt extract agar medium) were not significantly different in supporting mycelium growth of oyster mushroom PC. The optimal temperature for mycelium growth of both oyster mushroom species was obtained at $28^{\circ}C$. Mycelium growth of oyster mushroom PO was improved by carbon sources such as glucose, molasses, and at 1~5% sucrose concentration, mycelium colony diameter of mushroom PO was achieved the highest value. Whereas glucose, dextrose, and sucrose as carbon sources gave the good mycelium growth of oyster mushroom PC, and at 1~3% sucrose concentration, mycelium colony diameter of PC was achieved the maximum value. Ammonium chloride concentrations at 0.03~0.09% and 0.03~0.05% also gave the greatest values in mycelium colony diameter of mushroom PO and PC. Brown rice was found to be the most favourable for mycelium growth of two oyster mushroom species. In addition, sugarcane residue, acasia sawdust and corn cob were selected as favourable lignocellulosic substrate sources for mycelium growth of both oyster mushrooms.

One Alternative Process to Vapor Pressure Control for the Bulk Crystal Growth of GaAs

  • Oh, Myung-Hwan;Joo, Seung-Ki
    • Proceedings of the Korea Association of Crystal Growth Conference
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    • 1998.06a
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    • pp.149-156
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    • 1998
  • In this work, aiming at improvement of growth processes for the bulk GaAs single crystals, efforts have been made first in investigate thermodynamic properties of the Ga and As system and second to suggest that bulk GaAs crystals could be grown in principle with the single temperature zone only by determining the excess arsenic charge as a function of growth conditions. During crystal growth, this will be evaporized inside the growth chamber to induce the required inner pressure, instead of aesenic vapor pressure in the double temperature zone method, so as to be in equilibrium with the method, growth experiments have been prepared and carried out for dopes and undoped GaAs crystals with the newly built Bridgman system which was designed according to this principle. To compare the results to those of the double temperature zone method, the same numbers of GaAs crystals have been grown with both processes and all of them were characterized in single crystallinity, lattice defects and electrical properties. Especially, the relationship between growth conditions and crystal quality was discussed from the viewpoint of growth peculiarities with this method.

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