• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 심포지엄 논문집 정보 및 제어부문
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• pp.12-14
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• 2005

In this paper, we propose a new architecture of Self-Organizing Fuzzy Polynomial Neural Networks(SOFPNN) by means of genetically optimized fuzzy polynomial neuron(FPN) and discuss its comprehensive design methodology involving mechanisms of genetic optimization, especially genetic algorithms(GAs). The conventional SOFPNNs hinges on an extended Group Method of Data Handling(GMDH) and exploits a fixed fuzzy inference type in each FPN of the SOFPNN as well as considers a fixed number of input nodes located in each layer. The design procedure applied in the construction of each layer of a SOFPNN deals with its structural optimization involving the selection of preferred nodes (or FPNs) with specific local characteristics (such as the number of input variables, the order of the polynomial of the consequent part of fuzzy rules, a collection of the specific subset of input variables, and the number of membership function) and addresses specific aspects of parametric optimization. Therefore, the proposed SOFPNN gives rise to a structurally optimized structure and comes with a substantial level of flexibility in comparison to the one we encounter in conventional SOFPNNs. To evaluate the performance of the genetically optimized SOFPNN, the model is experimented with using two time series data(gas furnace and chaotic time series).

• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 학술대회 논문집 정보 및 제어부문
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• pp.146-148
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• 2005

In this paper, we propose a genetically optimized identification of information granulation(IG)-based fuzzy model. To optimally design the IG-based fuzzy model we exploit a hybrid identification through genetic alrogithms(GAs) and Hard C-Means (HCM) clustering. An initial structure of fuzzy model is identified by determining the number of input, the seleced input variables, the number of membership function, and the conclusion inference type by means of GAs. Granulation of information data with the aid of Hard C-Means(HCM) clustering algorithm help determine the initial paramters of fuzzy model such as the initial apexes of the membership functions and the initial values of polyminial functions being used in the premise and consequence part of the fuzzy rules. And the inital parameters are tuned effectively with the aid of the genetic algorithms and the least square method. And also, we exploite consecutive identification of fuzzy model in case of identification of structure and parameters. Numerical example is included to evaluate the performance of the proposed model.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 학술대회 논문집 정보 및 제어부문
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• pp.152-154
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• 2005

In this paper, we propose a new architecture of Fuzzy Polynomial Neural Networks(FPNN) by means of genetically optimized Fuzzy Polynomial Neuron(FPN) and discuss its comprehensive design methodology involving mechanisms of genetic optimization, especially Genetic Algorithms(GAs). The design of the network exploits the extended Group Method of Data Handling(GMDH) with some essential parameters of the network being provided by the designer and kept fixed throughout the overall development process. This restriction may hamper a possibility of producing an optimal architecture of the model. The proposed FPNN gives rise to a structurally optimized network and comes with a substantial level of flexibility in comparison to the one we encounter in conventional FPNNs. It is shown that the proposed genetic algorithms-based Fuzzy Polynomial Neural Networks is more useful and effective than the existing models for nonlinear process. We experimented with Medical Imaging System(MIS) dataset to evaluate the performance of the proposed model.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제6권1호
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• pp.33-38
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• 2006

In this paper, we propose a new architecture of Fuzzy Polynomial Neural Networks(FPNN) by means of genetically optimized Fuzzy Polynomial Neuron(FPN) and discuss its comprehensive design methodology involving mechanisms of genetic optimization, especially Genetic Algorithms(GAs). The conventional FPNN developed so far are based on mechanisms of self-organization and evolutionary optimization. The design of the network exploits the extended Group Method of Data Handling(GMDH) with some essential parameters of the network being provided by the designer and kept fixed throughout the overall development process. This restriction may hamper a possibility of producing an optimal architecture of the model. The proposed FPNN gives rise to a structurally optimized network and comes with a substantial level of flexibility in comparison to the one we encounter in conventional FPNNs. It is shown that the proposed advanced genetic algorithms based Fuzzy Polynomial Neural Networks is more useful and effective than the existing models for nonlinear process. We experimented with Medical Imaging System(MIS) dataset to evaluate the performance of the proposed model.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 하계학술대회 논문집 D
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• pp.2633-2635
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• 2003

In this study, we propose a fuzzy polynomial neural networks (FPNN) and a genetically optimized fuzzy polynomial neural networks(GoFPNN) for identification of non-linear system. GoFPNN architecture is designed by a FPNN based on fuzzy set and its structure and parameters are optimized by genetic algorithms. A fuzzy neural networks(FNN) based on fuzzy set divide into two structures that is simplified inference structure and linear inference structure. The proposed FPNN is resulted from integration and extension of simplified and linear inference structure of FNN. The consequence structure of the FPNN consist of polynomials represented by networks using connection weights for rules. The networks comprehend simplified(Type 0), linear (Type 1), and quadratic(Type 3) inferences. The proposed FPNN can select polynomial type of consequence part for each rule. Therefore, proposed scheme can offer flexible structure design capability for a system characteristics. Moreover, GAs is applied to networks structure and parameters tuning of proposed FPNN, and its efficient application method is discussed, these subjects are result in GoFPNN that is optimal FPNN. To evaluate proposed model performance, a numerical experiment is carried out.

• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.14-19
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• 2011

A cyclic undecapeptide-family natural product, cyclosporin A (CyA), which is one of the most valuable immunosuppressive drugs, is produced nonribosomally by a multifunctional cyclosporin synthetase enzyme complex in a filamentous fungal strain named Tolypocladium niveum. Previously, structural modifications of cyclosporins such as a regionspecific hydroxylation at the $4^{th}$ N-methyl leucine in a rare actinomycetes called Sebekia benihana were reported to lead to dramatic changes in their bioactive spectra. However, the reason behind this change could not be determined since a system to genetically manipulate S. benihana has not yet been developed. To address this limitation, in this study, we utilized the most commonly practiced gene manipulation techniques including conjugation-based foreign gene transfer-and-expression as well as targeted gene disruption to genetically manipulate S. benihana. Using these optimized genetic manipulation systems, a putative cytochrome P450 hydroxylase (CYP) gene named CYP506, which is involved in CyA hydroxylation in S. benihana, was specifically disrupted and genetically complemented. The S. benihana${\Delta}$CYP506 exhibited a significantly reduced CyA hydroxylation yield as well as considerable yield restoration by functional complementation of the S. benihana CYP506 gene, suggesting that the genetically manipulated S. benihana CYP mutant strains may serve as a more efficient bioconversion host for various valuable metabolites including CyA.

• 한국지능시스템학회논문지
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• 제14권5호
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• pp.660-665
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• 2004

In this study, a new architecture and comprehensive design methodology of genetically optimized Multi-layer Fuzzy Neural Networks (gMFNN) are introduced and a series of numeric experiments are carried out. The gMFNN architecture results from a synergistic usage of the hybrid system generated by combining Fuzzy Neural Networks (FNN) with Polynomial Neural Networks (PNN). FNN contributes to the formation of the premise part of the overall network structure of the gMFNN. The consequence part of the gMFNN is designed using PNN. The optimization of the FNN is realized with the aid of a standard back-propagation learning algorithm and genetic optimization. The development of the PNN dwells on the extended Group Method of Data Handling (GMDH) method and Genetic Algorithms (GAs). To evaluate the performance of the gMFNN, the models are experimented with the use of a numerical example.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2002년도 하계학술대회 논문집 D
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• pp.2176-2179
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• 2002

This paper introduces a hybrid induction motor control using a genetically optimized pseudo-on-line method. Optimization results from the use of a look-up table based on genetic algorithms to find the global optimum of a un-constraint optimization problem. The approach to induction motor control includes a pseudo-on-line procedure that optimally estimates parameters of a fuzzy PID (FPID) controller. The proposed hybrid genetic fuzzy PID (GFPID) controller is applied to speed control of a 3-phase induction motor and its computer simulation is carried out. Simulation results show that the proposed controller is performs better than conventional FPID and PID controllers. The contribution of this paper is the introduction of a high performance hybrid form of induction motor control that makes on-line and real-time control of the drive system possible.

• 대한의생명과학회지
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• 제25권4호
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• pp.398-406
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• 2019

Cancer immunotherapy using gene-modified tumor cells is safe and customized cancer treatment method. In this study, we made gene-modified tumor cells by transferring costimulatory molecules, 4-1BBL and OX40L, into tumor cells using lentivirus vector, and identified anti-cancer effect of gene-modified tumor cells in CT26 mouse colorectal tumor model. We construct pLVX-puro-4-1BBL, -OX40L vector for lentivirus production and optimized the transfection efficiency and transduction efficiency. The transfection efficiency is maximal at DNA:cationic polymer = 1:0.5 and DNA 2 ㎍ for lentivirus production. Then, the lentiviral including 4-1BBL and OX40L was used to deliver CT26 mouse tumor cells to establish optimal delivery conditions according to the amount of virus. The transduction efficiency is maximal at 500 μL volume of lentiviral stock without change in cell shape or growth rate. CT26-4-1BBL, CT26-OX40L significantly inhibited the tumor growth compare with CT26-WT or CT26-β-gal cell line. These data showed the possibility the use of genetically modified tumor cells with costimulatory molecule as cancer immunotherapy agent.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2004년도 학술대회 논문집 정보 및 제어부문
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• pp.346-348
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• 2004

In this rarer, we introduce a new Fuzzy Polynomial Neural Networks (FPNNs)-like structure whose neuron is based on the Fuzzy Set-based Fuzzy Inference System (FS-FIS) and is different from that of FPNNs based on the Fuzzy relation-based Fuzzy Inference System (FR-FIS) and discuss the ability of the new FPNNs-like structurenamed Fuzzy Set-based Polynomial Neural Networks (FSPNN). The premise parts of their fuzzy rules are not identical, while the consequent parts of the both Networks (such as FPNN and FSPNN) are identical. This difference results from the angle of a viewpoint of partition of input space of system. In other word, from a point of view of FS-FIS, the input variables are mutually independent under input space of system, while from a viewpoint of FR-FIS they are related each other. In considering the structures of FPNN-like networks such as FPNN and FSPNN, they are almost similar. Therefore they have the same shortcomings as well as the same virtues on structural side. The proposed design procedure for networks' architecture involves the selection of appropriate nodes with specific local characteristics such as the number of input variables, the order of the polynomial that is constant, linear, quadratic, or modified quadratic functions being viewed as the consequent part of fuzzy rules, and a collection of the specific subset of input variables. On the parameter optimization phase, we adopt Information Granulation (IG) based on HCM clustering algorithm and a standard least square method-based learning. Through the consecutive process of such structural and parametric optimization, an optimized and flexible fuzzy neural network is generated in a dynamic fashion. To evaluate the performance of the genetically optimized FSPNN (gFSPNN), the model is experimented with using gas furnace process dataset.