• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.4
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• pp.96-102
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• 2019

When GGBFS(Ground Granulated Blast Furnace Slag) with high blaine is incorporated in concrete, compressive strength in the initial period is improved, but several engineering problems arise such as heat of hydration and quality control. In this paper, compressive strength and durability performance of concrete with 3,000 Grade-low fineness slag are evaluated. Three conditions of concrete mixtures are considered considering workability, and the related durability tests are performed. Although the strength of concrete with 3,000 Grade slag is slightly lower than the OPC(Ordinary Portland Cement) concrete at the age of 28 days, but insignificant difference is observed in long-term compressive strength due to latent hydration activity. The durability performances in concrete with low fineness slag show that the resistances to carbonation and freezing/thawing action are slightly higher than those of concrete with high fineness slag, since reduced unit water content is considered in 3,000 Grade slag mixture. For the long-term age, the chloride diffusion coefficient of the 3000-grade slag mixture is reduced to 20% compared to the OPC mixture, and the excellent chloride resistance are evaluated. Compared with concrete with OPC and high fineness GGBFS, concrete with lower fineness GGBFS can keep reasonable workability and durability performance with reduced water content.

• Korean Journal of Remote Sensing
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• v.35 no.2
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• pp.327-336
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• 2019

The caldera lake "Chun-ji" is located at the summit of Baekdu volcano, which is in the border of China and North Korea. Chun-ji Lake has altitude 2,189 m above sea level. The Chun-ji is freezing in the winter when the water temperature goes down to zero for a year, and it melts in the season when the water temperature goes up again. However,since it is located at a high altitude, there are many cloudy days, and it is difficult to observe with optical images. For this reason, radar images, which are less influenced by weather than optical images, are more effective for observing the ice of heaven and earth. In this study, 75 TerraSAR-X images from chun-ji area were used for analysis from 2015 to 2017, and the calculated ice area and temperature changes were analyzed. As a result, the ice of the caldera lake formed was formed in early December and slowly melted until mid-April. During this period, temperatures in the Samjiyeon area were about $-10^{\circ}C$ when ice was produced, and the temperature was about $0^{\circ}C$ in mid-April when it was thawing. Correlation coefficients between ice surface area and temperature in winter 2015 and 2016, where global ice is produced,show a high correlation of -0.82 and -0.75. In addition to the results of this study, it can be used as an indicator to monitor the volcanic activity by comparing the result of the recent volcanic activity with the result of the increase in water temperature using various imagery.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.3
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• pp.611-619
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• 2021

Porous asphalt pavement (PAP) has many functions, such as reducing accidents and decreasing noise. On the other hand, vulnerability is inevitable because PAP contains approximately 20% porosity. This study evaluated the effects of the maximum aggregate size (MAS), temperature, and porosity on the PAP durability. The indirect tensile strength measures durability. This study tested the samples that stayed dry and were moisturized by freezing and thawing for mixtures having the same porosity of 20% and MAS of 13mm, 10mm, and 8mm. The same test was performed on a mixture of 20% and 22% voids made of the same material with a MAS of 10mm. As a result, for 20% porosity, significant differences in the changes in MAS and temperature were found. A clear difference was observed between 8mm and 13mm under dry conditions, but there were no other significant differences in the MAS change. Furthermore, there was a clear difference in temperature for the change in porosity and temperature, but the gap in 2% porosity at 20% did not show a clear difference. Therefore, it is necessary to develop a more durable PAP through quantitative evaluations of the factors affecting the PAP durability.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.42 no.5
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• pp.607-616
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• 2022

Corrosion and degradation of reinforced structures due to abnormal climates and natural disasters further accelerate the aging of structures. Coping with the decrease in structure performance, many old structures are being repaired and reinforced with low-weight and high-strength materials such as glass fiber composite material (GFRP). To further contribute, this paper focus on a more economical and eco-friendly material, basalt fiber composite (BFRP), which provide a more effective lateral constraint effect for seismic reinforcement. The main variables considered in this study are the curing temperature during the manufacturing of BFRP and the material characteristics of the target concrete member. The lateral constraint reinforcement effect was investigated through the evaluation of the performance of normal concrete and those with improved durability through fiber reinforcement. The reinforcement effect was 3.15 times for normal concrete and 3.72 times for fiber reinforced concrete, and the difference in reinforcement effect due to the improvement of the durability characteristics of the compression member was not significant. Lastly, the performance of the BFRP was compared with the results of the GFRP reinforcement from the previous study. The effect of the BFRP reinforcement was 1.18 times better than that of the GFRP reinforcement.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.187-192
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• 2022

The repeated monthly or weekly subculture of plant callus is labor intensive and increases the risk of somaclonal variation from the parental callus line. The most effective method for preserving plant callus is cryopreservation, which involves storage in liquid nitrogen. However, this method cannot be applied to the callus of different plant species in the same manner, so it is difficult to develop a standardized cryopreservation method. In addition, the survival rate of the frozen callus after thawing and the regeneration rate after survival are uncertain. Therefore, it is necessary to develop a method to extend the subculture interval of plant callus in an active state. In this study, active plant calli of various species without freezing was incubated at 15℃ for 4 to 12 weeks without subculture. After 12 weeks, 8 lines of plant callus grew less than 2-fold when cultured at 25℃, but at least 2 times as much when cultured at 15℃. Moreover, total antioxidant activity did not differ significantly between plant callus recovered at 25℃ after culturing at 15℃ or at 25℃. These results show that the subculture interval can be extended at a temperature of 15℃ without need for modified medium composition or additional processes. In addition, positive results in all calli of several plant species are expected to reduce labor as well as somaclonal variation by increasing the subculture.

• Journal of Conservation Science
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• v.36 no.6
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• pp.494-504
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• 2020

Ten historic sites (denoted as A- J in this study) of a tomb were found during the construction of the east-west expressway in District 2 of Hyangnam, Hwaseong, which is implemented by the Gyeonggi-do headquarters of the Korea Land & Housing Corporation. Thetombswere first detected at siteH, and further investigations revealed various tombs from the Three Kingdoms period; artifacts such as gilt-bronze shoes and caps were excavated from wooden coffins in the tombs. The pottery examined in this study was the only pottery artifact excavated from the site. Its raw clay was soft and loose, reddish brown, and had quartz and feldspar particles of < 1 mm, which appeared to have been added as reinforcing agents. The firing temperature of the pottery was estimated to be under 800-870 ℃ as the mica remains and tridimite, which is the phase transition mineral of quartz, was not produced; a slight endothermic peak was also detected because of the hydration of sericite at 800 ℃. The condition of the artifact was severely weakened because of various factors, such as soil pressure from the stratum formed over the site and repeated freezing and thawing. The artifact could not be collected alone, and thus, surrounding soil that had attached to the artifact was also collected; the artifact was transported to the laboratory and conservation treatment was conducted in a safe and systematic manner.

• Animal Bioscience
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• v.35 no.12
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• pp.1827-1838
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• 2022

Objective: The semen quality of stallions including sperm motility is an important target of selection as it has a high level of individual variability. However, effects of the molecular architecture of the genome on the mechanisms of sperm formation and their preservation after thawing have been poorly investigated. Here, we conducted a genome-wide association study (GWAS) for the sperm motility of cryopreserved semen in stallions of various breeds. Methods: Semen samples were collected from the stallions of 23 horse breeds. The following semen characteristics were examined: progressive motility (PM), progressive motility after freezing (FPM), and the difference between PM and FPM. The respective DNA samples from these stallions were genotyped using Axiom Equine Genotyping Array. Results: We performed a GWAS search for single nucleotide polymorphism (SNP) markers and potential genes related to motility properties of frozen-thawed semen in the stallions of various breeds. As a result of the GWAS analysis, two SNP markers, rs1141327473 and rs1149048772, were identified that were associated with preservation of the frozen-thawed stallion sperm motility, the relevant putative candidate genes being NME/NM23 family member 8 (NME8), olfactory receptor family 2 subfamily AP member 1 (OR2AP1), and olfactory receptor family 6 subfamily C member 4 (OR6C4). Potential implications of effects of these genes on sperm motility are herein discussed. Conclusion: The GWAS results enabled us to localize novel SNPs and candidate genes for sperm motility in stallions. Implications of the study for horse breeding and genetics are a better understanding of genomic regions and candidate genes underlying stallion sperm quality, and improvement in horse reproduction and breeding techniques. The identified markers and genes for sperm cryotolerance and the respective genomic regions are promising candidates for further studying the biological processes in the formation and function of the stallion reproductive system.

• Journal of The Geomorphological Association of Korea
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• v.17 no.4
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• pp.71-83
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• 2010

In this study, the volume of shoreline retreat at sea-cliffs in the Taean peninsula(West Coast of Korea) was estimated and their erosion and seasonal landforms characteristics changes were investigated through multi-temporal aerial photographs and field survey. Based on the analysis of aerial photographs through ortho-correction, the results show that the length of shoreline and erosion area increase as erosion at sea-cliffs occurs in Pado-li and Dundu-li. To obtain the seasonal quantitative landforms changes and retreat of sea-cliffs, we marked top, middle, and bottom datum-points, from which the distance to the nearest bedrock was repeatedly measured. In these regions, the retreat of sea-cliffs gradually increases in spring to summer, but gradually decreases in autumn. In particular, the typhoon that has a great influence on the Korean peninsula in July to September in summer would drastically increase the retreat of sea-cliffs in comparison with other seasons. As the outcrop of sea-cliffs repeats freezing and thawing in winter, the retreat of sea-cliffs increases a little due to active mechanical weathering. To know the erosion and seasonal landforms changes of sea-cliffs, we took pictures of them in every month and then analyze their condition. The retreat of sea-cliffs was repeatedly occurred by the circulation of the erosion of sea-cliff base, landslides, the formation of slope sediment debris and their erosion, in that order.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.72-72
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• 2003

The present study examined the possibility of cryopreservation of the D-shaped and umbo larvae of arkshell (Scapharca broughtonii), in terms of the survival rates after freezing and thawing. D-shaped and umbo larvae of arkshells were obtained from a shellfish farming on Yosu city. The average shell lengths were $93.3 \pm 10.1 \mu$m and $201.7 \pm 13.5 \mu$, respectively. Five cryoprotectants (CPAs), dimethyl sulfoxide (DMSO), glycerol, ethylene glycol (EG), propylene glycol (PG), and methanol, were tested at the concentrations of 1.5, 2.0 and 2.5 M. After larvae suspended in CPAs, cryoprotectants were loaded in 0.5 ml straws at a larval density of 50-100 larvae per straw, and epuilibrated for 10 and 20 minute at room temperature ($23^{\circ}C$), repectively. Straws were cooled at a rate of $1^{\circ}C$/min from $0^{\circ}C$ to $-12^{\circ}C$, held for 5 min at $-12^{\circ}C$, and then cooled at $2^{\circ}C$/min to $-35^{\circ}C$ and equilibrated for 5 min followed by plunging in liquid nitrogen. After storage in liquid nitrogen for 1 day, straws were thawed in a $30^{\circ}C$ water. As soon as straws were observed to melt, larvae were diluted with an equal volume of ASW and then washed twice with a large volume of ASW at an interval of 2 min to unload the CPAs. The results showed that after equilibration for 10 and 20 minute at room temperature, no larvae survived using methanol as CPAs, and it was observed that larval shells all open slightly, and larval flesh broke down and slopped over the shells. The highest survival rates (D-shaped larvae: 77.6%, umbo larvae: 59.3%) were obtained with 2M DMSO, and 1.5M glycerol yielded survival rates of 53.8% for D-shaped larvae and 37.5% for umbo larvae. The surviving D-shaped larvae showed active rotary motion and perfect membrane integrity and cytoplasmic normality, and the vigorous movement of veliger cilia was observed inside the closed shells. The breakdown of tissue occurred in the abnormal larvae, and the isolated cell often run out of shells.

• Journal of the Korean Geotechnical Society
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• v.22 no.10
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• pp.77-91
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• 2006

Domestic practices in shotcrete use have developed in many respects even now, but it still has issues about material, construction, quality standard and so on. In overseas, the construction using high strength shotcrete with $39.2{\sim}58.8 MPa$ of compressive strength is becoming common based on the shotcrete technology of high strength and durability. However, domestic shotcrete design strength is low at around 20.6 MPa of compressive strength and a long term durability is also insufficient. In this paper, field tests using high-quality additives and accelerators were performed to obtain the improvement of shotcrete strength and EFNARC standard was used to evaluate the field test results. In addition, deterioration test combined with the freezing-thawing and carbonation was also performed in order to investigate a long-term durability of high-strength shotcrete. As a result of the field test, the promotion ratio of early strength was $90{\sim}97%$ in case of using alkali-free accelerators. And the compressive strength of the shotcrete using Micro-silica fume was $45.2{\sim}55.8MPa$ and flexible strength was $5.01{\sim}6.66MPa$, so the promotion ratio of strength was $37{\sim}79%$ and $17{\sim}61%$ respectively. The promotion effect of strength by silica fine additives ratio of $7.5{\sim}10%$ for cement mass was much superior to the other cases. It was especially examined that using Micro-silica fume reduced deterioration due to mixed steel fiber and improved a long-term durability of shotcrete.