• Title/Summary/Keyword: fragment

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Specific DNA fragment analysis of Salmonella pullorum and S gallinarum by subtraction PCR (RDA method(Subtraction PCR) 기법을 이용한 닭의 Salmonella pullorum과 S gallinarum의 specific DNA fragment 분리 연구)

  • Park Jae-Myoung;Lee Jong-Jin;Choi Hae-Yeon;Jo Woo-Yeong;Lee Kyung-Hyeon;Song Jae-Chan
    • Korean Journal of Veterinary Service
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    • v.28 no.1
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    • pp.1-21
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    • 2005
  • Pullorum disease and Fowl typhoid are kind of poultry specific disease for poultry. The peculiar character of these poultry specific diseases is that it can be infected by transmitting vertically and horizontally, also it is hard to be discovered by clinical sign, and pathology or immunology. So, to develop the PCR method which distinguishes these two genetically similar diseases of separated the specific DNA fragment from each strain and use it for differential diagnosis by subtraction PCR method. Standard strain of S gallinarum and S pullorum, and field isolation strain were verified by biochemistry, It confirmed existence of plasmid by using the PFGE. Then, Isolated DNA from it and used it as materials for the experiment. After cutting genomic DNA of two strains by using Sau 3Al, It ligated primer to tester DNA for PCR amplification and separated specific DNA fragment bacteria with method of subtraction PCR. And, It confirmed that it is a piece of unique DNA in every bacteria using base sequence of separated DNA fragment. 1. The six specific DNA fragment were separated from the DNA of S gallinarum and S pullorum by the subtraction PCR method. 2. In the result of comparison after setting base sequence of each fragment, each separated base sequence of DNA fragment they did not correspond to each other 3. As the result of each DNA fragment is derived from the each strain of DNA, and there was no homology of genomic DNA level in mutual. 4. The fragment originated in plasmid and includes S pullorum did not separate. 5. In the result of searching base sequence in Genebank, it partially shows homology in Salmonella enterica, S typhimurium, S dublin, Escherichia coli, Shigella flexneri, Yersinia pestis, Klebsiella pneumoniae. 6. Primer design by S gallinarum DNA 2, 3 fragment used PCR, They are positive reaction in only S gallinarum at 276, 367 bp position.

Estimation of the Terminal Velocity of the Worst-Case Fragment in an Underwater Torpedo Explosion Using an MM-ALE Finite Element Simulation (MM-ALE 유한요소 시뮬레이션을 이용한 수중 어뢰폭발에서의 최악파편의 종단속도 추정)

  • Choi, Byung-Hee;Ryu, Chang-Ha
    • Explosives and Blasting
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    • v.37 no.3
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    • pp.13-24
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    • 2019
  • This paper was prepared to investigate the behavior of fragments in underwater torpedo explosion beneath a frigate or surface ship by using an explicit finite element analysis. In this study, a fluid-structure interaction (FSI) methodology, called the multi-material arbitrary Lagrangian-Eulerian (MM-ALE) approach in LS-DYNA, was employed to obtain the responses of the torpedo fragments and frigate hull to the explosion. The Euler models for the analysis were comprised of air, water, and explosive, while the Lagrange models consisted of the fragment and the hull. The focus of this modeling was to examine whether a worst-case fragment could penetrate the frigate hull located close (4.5 m) to the exploding torpedo. The simulation was performed in two separate steps. At first, with the assumption that the expanding skin of the torpedo had been torn apart by consuming 30% of the explosive energy, the initial velocity of the worst-case fragment was sought based on a well-known experimental result concerning the fragment velocity in underwater bomb explosion. Then, the terminal velocity of the worst-case fragment that is expected to occur before the fragment hit the frigate hull was sought in the second step. Under the given conditions, the possible initial velocities of the worst-case fragment were found to be very fast (400 and 1000 m/s). But, the velocity difference between the fragment and the hull was merely 4 m/s at the instant of collision. This result was likely to be due to both the tremendous drag force exerted by the water and the non-failure condition given to the frigate hull. Anyway, at least under the given conditions, it is thought that the worst-case fragment seldom penetrate the frigate hull because there is no significant velocity difference between them.

Techniques of XML Fragment Stream Organization for Efficient XML Query Processing in Mobile Clients (이동 클라이언트에서 효율적인 XML 질의 처리를 위한 XML 조각 스트림 구성 기법)

  • Ryu, Jeong-Hoon;Kang, Hyun-Chul
    • The Journal of Society for e-Business Studies
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    • v.14 no.4
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    • pp.75-94
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    • 2009
  • Since XML emerged as a standard for data exchange on the web, it has been established as a core component in e-Commerce and efficient query processing over XML data in ubiquitous computing environment has been also receiving much attention. Recently, the techniques were proposed whereby an XML document is fragmented into XML fragments to be streamed and the mobile clients receive the stream while processing queries over it. In processing queries over an XML fragment stream, the average access time significantly depends on the order of fragments in the stream. As such, for query performance, an efficient organization of XML fragment stream is required as well as the indexing for energy-efficient query processing due to the reduction of tuning time. In this paper, a technique of XML fragment stream organization based on query frequencies, fragment size, fragment access frequencies, and an active XML-based indexing scheme are proposed. Through implementation and performance experiments, our techniques were shown to be efficient compared with the conventional XML fragment stream organizations.

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Wordsworth's Re-Formation of Individuality: "Spots of Time," the Fragment and the Autobiography

  • Park, Mikyung
    • Journal of English Language & Literature
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    • v.56 no.6
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    • pp.1361-1378
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    • 2010
  • This paper argues that it is possible to construct an analogy of the literary fragment to an organic individual on the basis of an autonomous system of organic unity by reading William Wordsworth's ways of self-writing in The Prelude. The organicity of a fragment is borrowed from Friedrich Schlegel's theoretic and literary approach to the Romantic fragment. Focusing primarily on the two "spots of time" in Book Twelfth, I attempt to formulate a reciprocal relationship between a work of art and a literary autobiographer in terms of the self-generativity of the fragment. To be precise, both the fragment and the autobiographical project presuppose and at the same time depend on the engendering force of an organic unity and its resistance to discontinuity, which ironically affirms the persistent threat of disruption and death. Rewriting traumatic childhood experiences as rites of passage into adulthood, the two specified "spots of time" show the dominant mode of memory operative in the poem. Asserting the prominence of the individual as the very vehicle of realizing universal humanity, Wordsworth tries to re-form his individuality grounded in his childhood memories in a literary fashion. Under the premise that the poet is remembered by his posterity, The Prelude is constituted and reconstituted in conjunction with different versions of each memory. The poem also marks the poet's unachieved project of writing a philosophical poem, namely, The Recluse; for this very reason, The Prelude, which is complete in itself, points to an eternal work in progress, turning the truth of every fragment complete in its incompletion. As a trope of fragmentation, an autobiographical individuality is reformed in the poet's process of writing and re-visioning while simultaneously being dispersed once again between words, sentences, and pages.

Secure Fragmentation Technique for Content-Centric Networking (콘텐츠 중심 네트워크를 위한 안전한 패킷 단편화 기술)

  • Hyun, Sangwon
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.27 no.4
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    • pp.831-841
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    • 2017
  • This paper presents a secure and DoS-resistant fragment authentication technique for Content-Centric Networking (CCN). Our approach not only guarantees the authenticity of each fragment, but also provides a high resistance to DoS attacks through the immediate verification of fragment authenticity at interim nodes on the routing path. Our experimental results demonstrate that the proposed approach provides much stronger security than the existing approach, without imposing a significant overhead.

A New Approach to Fragment Assembly in DNA Sequencing

  • Pevzner, Pavel-A.;Tang, Haixu;Waterman, Micheal-S.
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • 2001.08a
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    • pp.11-35
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    • 2001
  • For the last twenty years fragment assembly in DNA sequencing followed the "overlap - layout - consensus"paradigm that is used in all currently available assembly tools. Although this approach proved to be useful in assembling clones, it faces difficulties in genomic shotgun assembly: the existing algorithms make assembly errors and are often unable to resolve repeats even in prokaryotic genomes. Biologists are well-aware of these errors and are forced to carry additional experiments to verify the assembled contigs. We abandon the classical “overlap - layout - consensus”approach in favor of a new Eulerian Superpath approach that, for the first time, resolves the problem of repeats in fragment assembly. Our main result is the reduction of the fragment assembly to a variation of the classical Eulerian path problem. This reduction opens new possibilities for repeat resolution and allows one to generate error-free solutions of the large-scale fragment assemble problems. The major improvement of EULER over other algorithms is that it resolves all repeats except long perfect repeats that are theoretically impossible to resolve without additional experiments.

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Reattachment procedure for treatment of fractured maxillary anterior teeth: two case reports (치관부 파절편 재부착술식을 이용한 상악전치부 치관파절의 치료에 대한 2건의 증례보고)

  • Choi, Yoorina
    • The Journal of the Korean dental association
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    • v.54 no.7
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    • pp.492-500
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    • 2016
  • Crown fractures are a common type of dental injuries and very frequently occurred on maxillary anterior teeth, area of requiring a rapid aesthetic recovery. Crown fragment reattachment is a simple and conservative method to restore the fractured teeth. The technique promotes esthetic outcomes as utilizing natural contour, shade, surface texture of teeth and gives mechanical similarity in terms of wear-resistance. Also it gives emotional positive responses to patients and requires less of chair time and costs. This case report presents two cases of crown fragment reattachments on maxillrary anterior teeth, including one complicated crown fracture and one uncomplicated crown fracture. If the fragment is available in cases of crown fractures, reattachment of fragment can be regarded as a predictable alternative. However, it is all the time important that a close conversation informing the patients about the limitations and prognoses of this treatment option.

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The Development of 20 mm Test Barrel with Replaceable Powder Chamber Type (약실교환방식의 20 mm 시험용 총열 개발)

  • Lee, Jin-Sung;Lee, Young-Shin
    • Journal of the Korea Institute of Military Science and Technology
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    • v.14 no.4
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    • pp.630-638
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    • 2011
  • A new smooth bore test barrel was developed to be used in sensitivity assessment test for explosives and fragment impact test. The bore diameter of the barrel is 20 mm, and the powder chamber is designed to be replaceable with the 12.7 mm, 20 mm and 30 mm type chamber. The test results showed the wide range of fragment velocity from 400 to 2000 m/s, included the fragment velocity requirement of the fragment impact test(alternate procedure #1) in MIL-STD-2105B. The stability of the bullet trajectory was checked by test shots and the structural safety of the system has been confirmed through the stress analysis and the interior ballistics analysis of the barrel.

HU Threshold Value for IV Catheter Fragment in Peripheral Vein of Volume Rendering 3D MDCT Imaging (정맥 내의 IV 카테터 조각을 3D MDCT 볼륨렌더링 영상으로 구현하기 위한 HU 임계치)

  • Jang, Keun-Jo;Kweon, Dae-Cheol
    • The Journal of the Korea Contents Association
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    • v.7 no.4
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    • pp.206-212
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    • 2007
  • To evaluate the HU value of the IV catheter fragment of CT on the accuracy and size in the peripheral vein. Pilot study of profile and table functions on PC by software was calculated of HU value of IV catheter fragment. This study demonstrates the utility of volume rendering technique to localize a small, subtle IV catheter, which can easily be reformatted of MDCT reformations. IV catheter fragment optimal image described as threshold range. Volume rendering of HU using a MDCT is an excellent method for evaluation the IV catheter fragment in three dimension.

RESTORATION OF A FRACTURED INCISOR USING ORIGINAL TOOTH FRAGMENT : A CASE REPORT (치아 파절편 재부착을 이용한 수복의 임상증례 보고)

  • Kim, Ji-Yeon;Park, Ki-Tae
    • Journal of the korean academy of Pediatric Dentistry
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    • v.24 no.2
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    • pp.475-483
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    • 1997
  • Fracture of the crown in a permanent incisor is relatively common. When it occurs with pulp exposure, it presents both restorative and endodontic problems. In the restoration of a fractured incisor, reattachment of the original fragment or restoration with a composite resin is preferred over a temporary crown. If fractured fragment is intact, the tooth can be restored with reattachment of the fragment. An exposed pulp in a young crown-fractured incisor is usually treated with either pulp capping or pulpotomy depending on the size of an exposure and time elapsed since injury. However, in teeth showing vital and/or hyperplastic pulp tissue at the exposure, only superficial layers of the pulp and surrounding dentin should be removed : i.e. partial pulpotomy can be performed in immature as well as mature teeth. This paper reports 2 cases of crown-fractured permanent incisors with pulp exposure that had been treated by reattachment of original fragment followed by partial pulpotomy or partial pulpectomy. The following results are obtained. ; 1. Fragment reattachment is an acceptable semi-permanent restoration of crown fractured young permanent incisor. 2. Partial pulpotomy is recommended as the treatment of choice in crown-fractured permanent teeth with pulp exposure.

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