• 생약학회지
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• 제54권1호
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• pp.27-37
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• 2023

This research was conducted to investigate the comprehensive effects of methanol extract of Coptidis rhizoma (MECR) against oral pathogen. We studied the antibacterial, anti-biofilm, anti-gingipain and anti-inflammatory activity of MECR. The minimum bactericidal concentration (MBC) of MECR was 100 ㎍/mL against several oral pathogens. The formation of biofilm of Streptococcus mutans was reduced to 8.93~24.12% in the presence of 25 ㎍/mL of MECR. The gingipain activity of Porphyromonas gingivalis were reduced to 3.91~6.23% in case of Kgp and 5.73~7.78% in case of Rgp in the presence of 10 mg/mL of MECR. The expression of fadA mRNA, virulence factor of Fusobacterium nucleatum (F. nucleatum) was 3 folds decreased in the presence of 25 ㎍/mL of MECR. In case of YD-38 cells challenged with F. nucleatum, RQ values of IL-8 and IL-6 were reduced about 12 folds and 5.45 folds in the presence of 2 ㎍/mL of MECR. In case of RAW 264.7 murine cell challenged with F. nucleatum, RQ values of IL-1β and IL-6 were 2.52 folds and 2.55 folds reduced in the presences of 2 ㎍/mL of MECR. Conclusively, MECR showed potent antibacterial and anti-inflammatory effects against oral pathogenic bacteria.

• Food Science and Biotechnology
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• 제18권5호
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• pp.1155-1160
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• 2009

Poly(lactide) (PLA)-coated paperboards were prepared by solution coating and thermo-compression coating methods and their effect of coating on the packaging properties such as tensile, water resistance, water vapor barrier, and heat sealing properties was tested. Compared with uncoated control paperboard, tensile strength (TS) of PLA-coated paperboard increased profoundly (2.2-2.6 folds) with slight increase in elongation at break (E). Water absorptiveness (WA) of the paperboard decreased 74-170 folds and water vapor permeability (WVP) decreased 6.3-22.1 folds by coating with PLA, which indicates an increase in the hydrophobicity of the surface of paperboard. Compared with polyethylene (PE)-coated paperboard, both PLA-coated paperboard exhibited 2.3 time higher heat sealing strength. In addition, the PLA-coated paperboards showed equal or higher wet TS than PE-coated paperboard. All the test results showed that the paperboard coated by the thermo-compression coating method was similar to or better than those of coated by the solution coating method.

• 자원환경지질
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• 제15권1호
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• pp.41-47
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• 1982

삼척탄전서부지역(三陟炭田西部地域)의 양단부(兩端部)에 해당하는 본연구지역(本硏究地域)은 태백산통(太白山統)을 기저(基底)로 하여 "조선-평안계(朝鮮-平安系)"의 층서(層序)가 해방전(解放前)에 수립(樹立)되었고 정창희(鄭昌熙)에 의하여 "평안계(平安系)"는 대폭 수정(修正)되었다. 특히 이 지역의 지질구조(地質構造)는 층서확립(層序確立)과 함께 "백운산대향사(白雲山大向斜)"구조(構造)가 이미 오래전에 인지(認知)되었으나 동서성(東西性)의 대습곡구조(大褶曲構造)와 남북성(南北性)의 교차습곡구조(交叉褶曲構造)와의 생성시기(生成時期)의 선후관계(先後關係)에 대해서는 다른 견해(見解)가 대립(對立)되어 왔다. 이 지역(地域)을 대향사(大向斜)의 남익부(南翼部)인 녹전구(碌田區), 북익부(北翼部)인 문곡구(文曲區), 비부(鼻部)인 예미구(禮美區)로 나누어 구조요소(構造要素)를 관측해석(觀測解析)하였던 바, 세 지역의 층이면(層理面)에 대한 ${\pi}$도(圖)에서 얻은 대원(大圓)의 극(極)과 선구조(線構造)의 최대치(最大値)는 비교적 잘 일치하는데 남북성(南北性) 교차습곡(交叉褶曲)의 경향(傾向)과 같다. 이는 남북성교차습곡(南北性交叉褶曲)의 형성과정(形成過程)에서 제구조요소(諸構造要素)가 생성(生成)되었고 동서성대향사(東西性大向斜) 구조(構造)와는 무관(無關)하다는 것을 입증(立證)하므로 선남북성교차습곡후동서성대향사(先南北性交叉褶曲後東西性大向斜)의 습곡작용(褶曲作用)이 있었음을 뒷받침하고 있다. 다시 말하면 본역(本域)에 발달(發達)하는 소구조요소(小構造要素)는 대체로 교차습곡(交叉褶曲)에 수반(隨伴)된 것으로 후기(後期)의 백운산향사(白雲山向斜)에 의해서 담란(擔亂)되고 있다. 기반암층(基盤岩層)에는 동사습곡(同斜褶曲)이, 하부고생층(下部古生層) 중의 규암층중(硅岩層中)에는 심한 단열(斷裂)이, 니질암층(泥質岩層) 중에는 각상습곡(角狀褶曲)이, 탄산염암층(炭酸鹽岩層)중에는 조합벽개(組合劈開)가, 상부고생층(上部古生層)중에는 평행습곡(平行褶曲)이 우세하여 층서(層序)에 따른 변형양식(變形樣式)의 차이(差異)를 보이고 있어 구조계층(構造階層)이 성립(成立)한다.

• 한국환경과학회지
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• 제24권1호
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• pp.55-64
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• 2015

The dry weight of mother plants' leaves had the highest increase rate in both NS (single-use) and NS+EM (mixed-use) mixed with NS 0.8 (customary use). In seafood amino acid fertilizer (SAF) application, the increase rate was highest in SAF solution at a 300-fold dilution. Mother plants' crown diameter, plant height, leaf length, leaf width, petiole length and leaf number showed the greatest growth amount when NS 0.8 (customary use) was mixed to NS (single-use) or NS+EM (mixed-use) solution. The growth was highest in SAF solution diluted 300 folds, but lowest in SAF solution diluted 100 folds. Of all inorganic nutrients, excluding sulfur, total amount of nitrogen, available phosphorus, potassium, calcium and magnesium had the highest increase rate in both NS (single-use) and NS+EM (mixed-use) with the treatment of NS 0.8 (customary use). Total nitrogen, in particular, was increased by 3.1% in NS 0.4, 6.0% in NS 0.8, and 4.5% in NS 0.8 with the application of NS+EM at a 500-fold dilution compared to NS alone. Total nitrogen amount showed the highest increase rate in SAF solution diluted 300 folds. Total nitrogen, available phosphorus, calcium, magnesium and EC in soils applied with culture solutions (NS, NS+EM) had increasing tendencies after fertilizer application. The results were comparable to those of SAF treatment. The increase rate of each inorganic nutrient composition declined in soils applied with NS+EM solution diluted 500 folds compared to NS alone.

• KSBB Journal
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• 제16권6호
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• pp.626-631
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• 2001

본 연구에서 L. crispatus KLB46을 저온 전처리함으로서 제제화 과정에 받게 되는 얼림과 녹임, 건조 스트레스뿐만 아니라, 여러 다른 환경 스트레스에 대한 내성이 증진된다는 것을 확인하였다. 그리고 내성 증진에 신규 단백질 합성이 필요함을 확인하였으며 나아가, 저온 충격 유전자 (csp)를 확인하였다. 따라서 이 균주를 제제화 하기 위한 방법으로 저온 전처리를 이용할 경우 생균력 유지에 큰 효과를 얻을 수 있다고 사료된다.

• 대한후두음성언어의학회지
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• 제11권1호
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• pp.76-80
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• 2000

Tongue-tip trill is a sound made by the tongue tip making contract with the alveolar ridge and oscillating rapidly as sound is produced. It is an exercise used by many singers to warm up the voice and used as one of the methods of voice rehabilitation for patients who have the vocal folds scarred postoperatively and also who present with a variety of disorders, particularly hypofunction and presbyphonia. We intended to investigate the mucosal vibration of the true vocal folds on tongue-tip trill by electroglottography and to find e effective methods of tongue-tip trill. One adult male volunteer participated. Spectrography and electroglottography were checked repeatedly 15 times, more than 5 second in each times, at same pitch, in three conditions of phonation : sustained /a/ vowel, anterior trill in which tongue-tip vibrated at anterior portion of alveolar ridge just behind the anterior tooth, and posterior trill in which at palatal crest behind the transverse palatine fold We measured the first and second formant to determine indirectly the position of tongue and calculated speed quotient and the ratio of closing phase to closed phase. Speed quotients of posterior trill were higher than sustained /a/ vowel and anterior trill in 14 times. The ratio of closing phase to dosed phase of posterior trill were lower than the others in 14 times. Mucosa of true vocal folds is vibrated more effectively on posterior trill rather than sustained /a/ vowel and anterior trill. So, when tongue-tip trill is used as a method of voice rehabilitation, we suggest that posterior trill is better in producing effective mucosal vibration

• 대한수의학회지
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• 제24권1호
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• pp.69-77
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• 1984

Gross and histological findings were obtained on cysts formed in the reproductive organs, particularly in the mesosalpinx, mesovarium, and fimbria, of 144 gilts and 37 sows. Of the 181 gilts and sows, 63 (34.8%) were found to have cysts which totaled 89. The number of cysts totaled 38 for the gilts and 51 for the sows, with relative frequencies of 18.1% and 48.6%, respectively. The total number of cysts and the incidence were greater in the sows than in the gilts. With regard to the location of cysts, the highest incidence was found in the mesosalpinx (59.6%). The incidence of cysts in the mesovarium and fimbria was relatively low, with 16.9% and 23.6%, respectively. Most cysts were spherical or oval in shape, and below 5.9mm in diameter. A pedunculated cyst was also seen. The bilateral, incidence of the cysts was lower than unilateral, although the incidence was approxmately the same between the gilts and the sows. With regard to the sexual cycle, the high incidence of cysts was found in the follicular phase than in the luteal phase. The cysts putting pressure upon the oviduct were classified into 3 types according to the shape of epithelium and lamina propria. In type I the epithelium had ciliated cells and the lamina propria formed folds. I type 2 ciliated cells existed, but no folds were formed. In type 3, neither ciliated cells nor folds were seen, the histological change, including partial atrophy and lack of folds in the tubal mucosa were seen.

• 암석학회지
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• 제6권1호
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• pp.45-51
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• 1997

중생대 시기의 조구조 운동은 중국 동부 서태평양지역의 구조 발달사에 중요한 역할을 한 운동이다. 연산지역은 중생대의 전형적인 구조가 형성, 노출된 곳이며 쥬라기-백악기에 발생한 연산운동의 명명지이다. 과거에는 이 지역 대부분의 구조들은 연산운동에 의해 형성된 것으로 일반적으로 인정되어 왔지만, 다수의 습곡들은 삼첩기 후반에 발생한 인지운동에 의해 형성되었고 동시에 많은 대다수 단층들을 형성한 강렬한 활동이 있었음이 최근의 종합적 연구에 의해 밝혀졌다. 인지 운동이 발생한 조구조의 동역학적 환경은, 중·한지괴(Sino-Korean Massif)가 북쪽으로 이동하면서 대체로 E-W방향인 흥안-몽골 습곡대에 부딛쳐 생성된 N-S 방향의 압축응력 체제하에서 닫침 습곡(그 중 일부는 역전 습곡)이 형성되었으며, 연산운동의 몇차례 변형이 비공축(non-coaxial)으로 중첩(surperposed)되었다. 연산지역에서 중생대의 단층활동의 주요한 특성으로 인지운동시기에는 압축응력 작용에 의한 드러스트(thrust)가 주로 형성되었고, 연산운동시기에는 압축전단응력에 의한 사교 드러스트(oblique-thrust)가 주로 형성되었다.

• 대한후두음성언어의학회지
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• 제31권2호
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• pp.66-70
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• 2020

Background and Objectives Presbyphonia is characterized by hoarse, breathy, weak vocal intensity. Extracellular matrix (ECM) in lamina propria (LP) of the vocal folds play an important role in voice production, and change of ECM according to the aging leads to the presbyphonia. The aim of this study was to investigate the histologic analysis of aging vocal fold of rat. Materials and Method Six and twenty two months old Sprague-Dawley rats (n=8, each group) were used and classified into young (six months old rats) and old (twenty two months old rats) group. Histologic analysis and immunohistochemical staining for ECM of LP were performed. Results Overall cellular density was significantly decreased in old rat group. Elastin fibers of LP were significantly decreased in old rat group. Type I collagen was significantly increased in old rat group. Type III collagen did not show significant difference. Hyaluronic acids did not show significant difference in Alcian blue staining and immunohistochemical staining. Conclusion Decreased general cellular density and elastin fiber and increased type I collagen were observed in the LP of vocal folds of aging rats. These ECM changes might to contribute the aging voice.

• Journal of Ginseng Research
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• 제45권4호
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• pp.473-481
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• 2021

Background: Mitochondrial dysfunction is one of the significant reasons for Alzheimer's disease (AD). Ginsenosides, natural molecules extracted from Panax ginseng, have been demonstrated to exert essential neuroprotective functions, which can ascribe to its anti-oxidative effect, enhancing central metabolism and improving mitochondrial function. However, a comprehensive analysis of cellular mitochondrial bioenergetics after ginsenoside treatment under Aβ-oxidative stress is missing. Methods: The antioxidant activities of ginsenoside Rb₁, Rd, Re, Rg₁ were compared by measuring the cell survival and reactive oxygen species (ROS) formation. Next, the protective effects of ginsenosides of mitochondrial bioenergetics were examined by measuring oxygen consumption rate (OCR) in PC12 cells under Aβ-oxidative stress with an extracellular flux analyzer. Meanwhile, mitochondrial membrane potential (MMP) and mitochondrial dynamics were evaluated by confocal laser scanning microscopy. Results: Ginsenoside Rg₁ possessed the strongest anti-oxidative property, and which therefore provided the best protective function to PC12 cells under the Aβ oxidative stress by increasing ATP production to 3 folds, spare capacity to 2 folds, maximal respiration to 2 folds and non-mitochondrial respiration to 1.5 folds, as compared to Aβ cell model. Furthermore, ginsenoside Rg1 enhanced MMP and mitochondrial interconnectivity, and simultaneously reduced mitochondrial circularity. Conclusion: In the present study, these results demonstrated that ginsenoside Rg₁ could be the best natural compound, as compared with other ginsenosides, by modulating the OCR of cultured PC12 cells during oxidative phosphorylation, in regulating MMP and in improving mitochondria dynamics under Aβ-induced oxidative stress.