• Parasites, Hosts and Diseases
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• 제49권2호
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• pp.173-175
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• 2011

Ophthalmomyiasis rarely occurs worldwide, and has not been reported in Korea. We present here a case of ophthalmomyiasis caused by Phormia sp. fly larva in an enucleated eye of a patient. In June 2010, a 50-year-old man was admitted to Dankook University Hospital for surgical excision of a malignant melanoma located in the right auricular area. He had a clinical history of enucleation of his right eye due to squamous cell carcinoma 5 years ago. During hospitalization, foreign body sensation developed in his right eye, and close examination revealed a fly larva inside the eye, which was evacuated. The larva was proved to be Phormia sp. based on the morphology of the posterior spiracle. Subsequently, no larva was found, and the postoperative course was uneventful without any complaints of further myiasis. This is the first case of ophthalmomyiasis among the literature in Korea, and also the first myiasis case caused by Phormia sp. in Korea.

• Animal Systematics, Evolution and Diversity
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• 제33권1호
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• pp.56-59
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• 2017

The external anatomy of the immature stages (last instar larva and pupa) of the crane fly, Tipula (Nippotipula) sinica Alexander, 1935 (Diptera: Tipulidae) is described and illustrated from specimens first collected in Korean peninsula (South Korea). Comments concerning natural history and microhabitats of larvae are provided. This is the first detailed description with illustrations for the last instar larva and pupa of Tipula (Nippotipula) sinica. Pupal characteristics resemble those of most species from advanced lineages within the subfamily Tipulinae. And, also it described with habitats and biological notes of Tipula (Nippotipula) sinica. Crane fly larvae, categorization as found in this survey indicate a taxonomic stream and are expected to help.

• 한국균학회지
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• 제44권4호
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• pp.355-359
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• 2016

During a survey of fungal diversity in insect guts in Korea, two fungal strains, EML-PGH2 and EML-PUKI88, were isolated from the gut of grasshopper and soldier fly larvae inhabiting the bulrush plants at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. Based on their morphological characteristics and a phylogenetic analysis of the internal transcribed spacer (ITS1 and ITS2) and 5.8S rDNA sequences, the strains were identified as Blakeslea trispora. To our knowledge, the zygomycete species B. trispora has not been previously described in Korea.

• Mycobiology
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• 제44권4호
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• pp.310-313
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• 2016

While surveying the diversity of fungi of the order Mucorales, two isolates, EML-PUKI12-1 and EML-PUKI06-1, were obtained from the gut of soldier fly larvae inhabiting the bulrush at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. The isolates were confirmed as Mucor irregularis and Mucor fragilis species, respectively, based on the morphological characteristics and phylogenetic analysis of rDNA internal transcribed spacer region. Such mucoralean species belonging to undiscovered taxa has not previously been described in Korea.

• 대한의생명과학회지
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• 제26권3호
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• pp.217-225
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• 2020

Scuttle fly which moves abruptly after standing for a while and stop suddenly to rush off again, is a fly species in the Phoridae family. This species like rotten organic materials and it is known to proliferate even in the industrial materials including organic solvents. These characteristic behaviors of the scuttle fly seem to be related to muscular and nervous system or neurotransmitters. Thus, we focused at the neurotransmitter, corazonin (Crz) that is known to be related to resistance to stress and investigated the developmental process of the neurons in the scuttle fly. Corazonin is a neuropeptide being expressed in the central nervous system (CNS) and is known to control mainly physiological functions and behaviors. Its many functions that have been proposed are still in controversy. In this studies, we found that there are three groups of corazoninergic neurons in the larval CNS of the scuttle fly and these neurons undergo distinguishable changes through metamorphic process compared to different fly species. Larva has 3 pairs of Crz neurons at the dorsolateral area of the brain, 1 pair at the dorsomedial brain and 8 pairs at the ventral nerve cord.

• 대한의생명과학회지
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• 제24권2호
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• pp.125-129
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• 2018

Megaselia scalaris, also known as the scuttle fly, has a humpbacked morphology and was categorized as an insect undergoing complete metamorphosis. Megaselia scalaris was reported to be found in diverse environments. Recently, the scuttle fly has been a focus of studies by forensic scientists, geneticists, and developmental biologists. However, detailed research into scuttle fly development is still unclear. Therefore, the current study has been performed to elucidate the development of Megaselia scalaris. The scuttle fly undergoes four distinct stages of development: egg, larva, pupa and adult fly. After mating, the scuttle fly eggs hatch within one day and become larvae. The newly generated larvae go through 1, 2, and 3 instar stages within a period of 5~10 days. The instar stage 1 takes 1~2 days, instar stage 2 takes 1~2 days, and instar stage 3 takes 3~5 days. Depending on the environmental temperature, the pupae stage takes approximately 10~15 days to complete. The pupa stage can be divided into five sub-stages: 1~3 days, 4~6 days, 7~9 days, 10~12 days and 13~15 days. After molting, the newly formed adult scuttle fly can fly after one day. The morphological characteristics of the scuttle fly during each stage of development was detailed within this study.

• 대한의생명과학회지
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• 제29권4호
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• pp.330-335
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• 2023

The corazonin (Crz) gene showed two subtypes of different length at laval and pupal stage. The long subtype fade out in adult central nerve system (CNS) but the short one survive through all the life cycle from larva to adult. The short subtype has the same base sequences with mature Crz mRNA and detected in both brain and ventral nerve cord (VNC). The long one, on the contrary, was detected only in the brain tissue. As observed in above results, Crz neurons develop in different pattern in the CNS of scuttle fly and the Crz gene expresses two different subtypes. These results suggest that this neurotransmitter may perform differential neurophysiological functions in the scuttle fly. Variation in the amino acid composition of the final active undecapeptide supports in strong those possibilities. We expect further studies on the relationship between neurophysiological functions of Crz and behavioral characteristics of the scuttle fly.

• 대한의생명과학회지
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• 제27권4호
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• pp.239-247
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• 2021

The scuttle fly is a fly species in the Phoridae family. Scuttle fly which moves abruptly after standing for a while and stop suddenly to rush off again. These characteristic behaviors of the scuttle fly seem to be related to muscular and nervous system or neurotransmitters. Thus, we focused at the neurotransmitter, corazonin (Crz) that is known to be related to resistance to stress and investigated the developmental process of the neurons in the scuttle fly. In a previous studies, we found that there are three groups of corazoninergic neurons in the larval CNS of the scuttle. Larva has 3 pairs of Crz neurons at the dorsolateral area of the brain, 1 pair at the dorsomedial brain and 8 pairs at the ventral nerve cord. In this studies, among these neurons, 1 pair of dorsomedial brain and 8 pairs of ventral nerve cord disappear in early pupal stage after metamorphosis. Only the 3 pairs of dorsolateral brain persist expression of Crz gene through all the period of pupa stage. This group of neurons converge gradually to frontal center of the brain and situated at the medial region. These pairs of corazoninergic neurons keep their number and location in adult stage. In the future, we expect further studies on the histological characteristics of corazonin-expressing cells and the expression of corazonin gene.

• 한국잠사곤충학회지
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• 제52권2호
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• pp.142-146
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• 2014

최근 곤충자원을 이용하여 식약용 및 사료로 활용하기 위한 많은 연구가 진행되고 있으며 미래의 자원으로 발전할 수 있는 가능성이 충분하다고 본다. 특히, 대량으로 생산이 용이하고 유충과 번데기의 조단백질 및 무기물 함량이 우수한 아메리카동애등에는 양계 및 양어사료의 원료로써 사용 가능성이 높게 평가되고 있다. 본 연구는 아메리카동애등에의 유충 및 번데기의 항산화 활성을 비교 평가하였다. 아메리카동애등에 번데기의 물 추출물의 경우 DPPH 라디칼 소거능이 $147.8{\pm}1.6%$로 양성 대조군으로 사용된 Vitamin C(5 mg/mL)의 $109.0{\pm}0.15%$와 메뚜기의 $109.5{\pm}28%$ 보다 높은 활성을 나타내었다. FRAP을 통한 환원력 측정에서도 DPPH 결과와 마찬가지로 아메리카동애등에 번데기의 물 추출물에서 가장 높은 환원력을 나타내었다. 위의 결과로 보아, 아메리카동애등에 유충 및 번데기의 추출물은 라디칼 소거능 및 산화환원작용을 통하여 항산화 활성을 가지는 것으로 사료되며, 특히 번데기의 추출물은 천연 항산화 소재로서의 활용가능성이 높은 것으로 기대되었다. 추후 추출물의 산업적인 활용가능성을 보다 명확히 확인하기 위하여 보다 많은 연구가 필요할 것으로 본다.

• International Journal of Industrial Entomology and Biomaterials
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• 제21권2호
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• pp.185-187
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• 2010

Most studies of the black soldier fly, Hermitia illucens, widely distributed throughout Southeast Asia and America focused on the use of larvae for recycling various organic materials and feed for fish and livestock. To recognize the instars of the soldier fly's larvae, we examined the number of exuviae originated from each larva from 1st instar to pupa. The weight and the head capsule width of the black soldier fly larvae also confirmed that the black soldier fly was passed through 6th instars. In addition, we found that the black soldier fly larvae had a well developed mandibular-maxillary complex, similar to those reported from the larvae of scavengers including Odontomyia cincta, Neopachygaster maculicornis, Ptecticus brunettii and Stratiomys ruficornis.