Mycobiology

The Korean Society of Mycology (한국균학회)
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Characterization of Two New Records of Mucoralean Species Isolated from Gut of Soldier Fly Larva in Korea

  • Nguyen, Thi Thuong Thuong (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Duong, Tham Thi (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University)
  • Received : 2016.10.31
  • Accepted : 2016.11.16
  • Published : 2016.12.30
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Abstract

While surveying the diversity of fungi of the order Mucorales, two isolates, EML-PUKI12-1 and EML-PUKI06-1, were obtained from the gut of soldier fly larvae inhabiting the bulrush at a pond located in the Chonnam National University Arboretum, Gwangju, Korea. The isolates were confirmed as Mucor irregularis and Mucor fragilis species, respectively, based on the morphological characteristics and phylogenetic analysis of rDNA internal transcribed spacer region. Such mucoralean species belonging to undiscovered taxa has not previously been described in Korea.

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References

  1. Micheli PA. Nova plantarum genera juxta Tournefortii methodum disposita. Florence: Typis Bernardi Paperinii; 1729.
  2. Schipper MA. On certain species of Mucor with a key to all accepted species. Stud Mycol 1978;17:1-52.
  3. Benny GL. Methods used by Dr. R. K. Benjamin, and other mycologists, to isolate zygomycetes. Aliso 2008;26:37-61. https://doi.org/10.5642/aliso.20082601.08
  4. Li GJ, Hyde KD, Zhao RL, Hongsanan S, Abdel-Aziz FA, Abdel-Wahab MA, Alvarado P, Alves-Silva G, Ammirati JF, Ariyawansa HA, et al. Fungal diversity notes 253-366: taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers 2016;78:1-237. https://doi.org/10.1007/s13225-016-0366-9
  5. Nguyen TT, Lee SH, Bae S, Jeon SJ, Mun HY, Lee HB. Characterization of two new records of zygomycete species belonging to undiscovered taxa in Korea. Mycobiology 2016;44:29-37. https://doi.org/10.5941/MYCO.2016.44.1.29
  6. Jacobs K, Botha A. Mucor renisporus sp. nov., a new coprophilous species from Southern Africa. Fungal Divers 2008;29:27-35.
  7. Walther G, Pawlowska J, Alastruey-Izquierdo A, Wrzosek M, Rodriguez-Tudela JL, Dolatabadi S, Chakrabarti A, de Hoog GS. DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia 2013;30:11-47. https://doi.org/10.3767/003158513X665070
  8. Alves MH, Campos-Takaki GM, Porto AL, Milanez AI. Screening of Mucor spp. for the production of amylase, lipase, polygalacturonase and protease. Braz J Microbiol 2002;33:325-30.
  9. Kendrick A, Ratledge C. Desaturation of polyunsaturated fatty acids in Mucor circinelloides and the involvement of a novel membrane-bound malic enzyme. Eur J Biochem 1992;209:667-73. https://doi.org/10.1111/j.1432-1033.1992.tb17334.x
  10. Ahn GR, Kwon HW, Ko HK, Kim SH. Unrecorded fungal species isolated from greenhouses used for shiitake cultivation in Korea. Kor J Mycol 2016;44:8-15. https://doi.org/10.4489/KJM.2016.44.1.8
  11. Lee YS, Jung HY, Lee HB, Kim SH, Shin KS, Eom AH, Kim C, Lee SY; Korean Society of Mycology. National list of species of Korea. Ascomycota, Glomeromycota, Zygomycota, Myxomycota, Oomycota. Incheon: National Institute of Biological Resources; 2015.
  12. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego (CA): Academic Press; 1990. p. 315-22.
  13. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82. https://doi.org/10.1093/nar/25.24.4876
  14. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95-8.
  15. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  16. Lu XL, Najafzadeh MJ, Dolatabadi S, Ran YP, Gerrits van den Ende AH, Shen YN, Li CY, Xi LY, Hao F, Zhang QQ, et al. Taxonomy and epidemiology of Mucor irregularis, agent of chronic cutaneous mucormycosis. Persoonia 2013;30:48-56. https://doi.org/10.3767/003158513X665539
  17. Bainier M. Nouvelles observations sur les zygospores des Mucorinees. Ann Sci Nat Bot 1884;19:200-14.
  18. Christenberry GA. A taxonomic study of the Mucorales in the Southeastern United States. J Elisha Mitchell Sci Soc 1940;56:333-66.