• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.114-114
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• 2018

Among the various volatile organic compounds (VOCs) emitted by the plant, floral scent plays a key role in attracting pollinators for reproduction and mediates ecological interactions. Floral scent is an important trait and industry drives the competition for flowers with novel scents. Chrysanthemum is one of the well-known ornamental plants and is a popular cut flower across the world. Floral scent and the genes responsible for the floral scent emission are poorly studied in chrysanthemum. In the present study, floral scent and the expression pattern of floral scent genes were analyzed in two chrysanthemum cultivars 'Golden Egg' and 'Gaya Glory'. Initially, intensity of the floral scent in five developing stages of flower including 'budding (B), bud developing (BD), initial blooming (IB), almost open (AO) and open flower (OF)' was analyzed using electronic nose (E-nose) with six metal oxide sensors. Based on the distance analysis, different stages of flower showed different relative intensity of scent according to the sensory evaluation. Although the scent pattern differed by stage, scent intensity was strongest in the OF stage in the completely opened flower in both the cultivars. Further, expression pattern of six genes in the floral scent pathway including FDS, IDI, ISPH, TPS2, TPS5 and TPS6 was observed in all the five stages of the flower in both the cultivars. The expression pattern of all the six genes differed by stage and the terpene synthase genes TPS2, TPS5 and TPS6 showed good expression levels in the $5^{th}$ flower stage compared to other stages. This study provides a preliminary data for understanding the regulation of floral scent in chrysanthemum.

• Journal of Ecology and Environment
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• v.46 no.1
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• pp.18-30
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• 2022

Background: Bees and flowering plants associations were initially began during the early Cretaceous, 120 million years ago. This coexistence has led to a mutual relationship where the plant serves as food and in return, the bee help them their reproduction. Animals pollinate about 75% of food crops worldwide, with bees as the world's primary pollinator. In general, bees rely on flower scents to locate blooming flowers as visual clue is limited and also their host plants from a distance. In this review, an attempt is made to collect some relevant 107 published papers from three scientific databases, Google Scholar, Scopus, and Web of Science database, covering the period from 1959 to 2021. Results: Flowering plants are well documented to actively emit volatile organic compounds (VOCs). However, only a few of them are important for eliciting behavioral responses in bees. In this review, fifty-three volatile organic compounds belonging to different class of compounds, mainly terpenoids, benzenoids, and volatile fatty acid derivatives, is compiled here from floral scents that are responsible for eliciting behavioral responses in bees. Bees generally use honest floral signals to locate their host plants with nectar and pollen-rich flowers. Thus, honest signaling mechanism plays a key role in maintaining mutualistic plant-pollinator associations. Conclusions: Considering the fact that floral scents are the primary attractants, understanding and identification of VOCs from floral scent in plant-pollinator networks are crucial to improve crop pollination. Interestingly, current advances in both VOCs scent gene identification and their biosynthetic pathways make it possible to manipulate particular VOCs in plant, and this eventually may lead to increase in crop productivity.

• Horticultural Science & Technology
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• v.34 no.1
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• pp.163-171
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• 2016

We investigated the intensity and pattern of the scent produced by diploid and tetraploid Cymbidium flowers, using an electronic nose with 6 metal oxide sensors (MOS). The MOS responses were evaluated by principal component analysis, discriminant function analysis, and sensor data. These analyses revealed that tetraploid flowers had a stronger scent than diploid flowers in Cymbidium Golden Elf 'Sundust'. Furthermore, among the different flower parts-column, lip, and petals-the column produced the strongest scent. There was no significant difference between the flowering periods of diploid and tetraploid potted Cymbidium Golden Elf 'Sundust' and Cymbidium Elma 'Orient Toyo' grown in a greenhouse. Moreover, there were no significant differences between the number of flowers per flower stem and the length of flower stems on the diploid and tetraploid plants of these two Cymbidium cultivars. This study provides potentially useful information for the breeding of polyploidy Cymbidium in the floriculture industry.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.1-11
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• 2010

Floral scent emitted from many plants is the key factor for pollinator attraction and defense for survival in nature and is important industrial materials for perfumery as well. It is a complex mixture of various organic molecules with a high volatility or a high vapor pressure. In general, floral scents are divided into three categories, aliphatics, terpenoids, and phenylpropanoids/benzenoids, based on its origin. About 1,700 scent compounds have been identified and their biochemistry and molecular biology also have elucidated their biosynthesis from various flowering plants during the last ten years. In addition to improvement of vase life, flower color and shape, and/or disease resistance, floral scent is coming up to the major breeding target for improvement of marketability. Therefore, metabolic engineering can be an important tool in near future and may be able to facilitate the breeding program for novel cultivar selection and improvement of marketability of floricultural crops.

• Journal of Practical Agriculture & Fisheries Research
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• v.17 no.1
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• pp.21-43
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• 2015

Korean floricultural industry has grown under the full backing of government, as it placed itself as the main export item since 2000s. Despite its high-speed growth after the domestic production scale of trillion won, the floricultural industry lost its pace after 2005, due to the market-opening of agricultural product and global economic depression. Although the national income is growing and the level of civilized living is following, spending on flowers is faltering. As of such circumstances, necessity for analyzing flower consumption trend and behavior has came out, along with the calls for expansion plan of flower consumption, under the basis of result analysis on consumer reports. The result of the research contains the trend/consumption behavior analysis on domestic and foreign flower industry to boost floricultural industry, along with reviews on various studies of the developmental process of domestic and foreign cut-flowers/pot-plants consumption trend. Also this study has reached to the various recognition of people toward flower, through a public survey. Through such results, this study would like to propose the measures for diffusion of flower-consumption culture, achieving the improvement of life-quality of public along. First, efforts on reviewing the customer accessibility is essential to create flower- culture and the consumption. Second, to eliminate the obstacles that prevents flowers from public's daily life is essential. Third is the effective public-relations on flowers. To create consuming culture and to adhere the positive images, it is essential to research and systematically organize categories of color, scent, and elements of flower and utilize them. Last proposal is the organization of flower-concerned personnels and the leading groups. The change is affected by the economical, social environment, along with the emotions of consumers. Therefore the necessity for the leading group to be the control-tower of such changes are very clear.

• Horticultural Science & Technology
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• v.30 no.6
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• pp.617-625
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• 2012

Tree peony is a traditional famous flower of China, and plays an important role in Chinese traditional culture. But the floral scent of tree peony in vivo is little known. In this study, in order to explore the floral composition of tree peony, floral volatiles of six cultivars, including Paeonia suffruticosa 'Zhaofen' (ZF), P. suffruticosa 'Luoyanghong' (LYH), P. ostii 'Fengdanbai' (FDB), P. ${\times}$ lemonei 'High noon' (HN), P. ${\times}$ lemonei 'Renown' (R), and P. rockii 'Gaoyuanshenghuo' (GYSH) were collected by dynamic headspace and then identified by Automated Thermal Desorption-Gas Chromatography/Mass Spectometry. The results showed that floral fragrances of the six cultivars were qualitatively and quantitatively distinct. A total of 105 volatiles involving ten categories were detected. But not all volatile categories were emitted from these cultivars. The six peony cultivars emitted some shared compounds and peculiar compounds. The total released amounts of volatiles emitted from six cultivars were found significantly different, which was greatest for 'GYSH'. The most abundant volatile compounds detected from 'ZF', 'LYH', 'FDB', 'HN', 'R', and 'GYSH' were respectively ${\alpha}$-pinene, 2,3-dihydroxy propanal, 3-methyl-1-butanol, 2-ethyl-1-hexanol, acetic acid 1-methylethyl ester, and 5-ethyl-2,2,3-trimethyl heptane. This result may contribute to exploring the biosynthesis and emission mechanism of floral scent in tree peony.

• Journal of the HCI Society of Korea
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• v.10 no.1
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• pp.29-41
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• 2015

Technologies for video reality (e.g., 3D displays, vibration, surround sound, etc.) utilize various sensory input and many of them are now commercialized. However, when it comes to the use of olfaction for video reality, there has not been much progress in both practical and academic respects. Because olfactory sense is tightly associated with human emotion, proper use of this sense is expected to help to achieve a high degree of video reality. This research tested the effects of a video's color-temperature related scent on reality improvement when the video does not have apparent object (e.g., coffee, flower, etc.) which suggest specific smell. To this end, we had participants to rate 48 scents based on a color-temperature scale of 1,500K (warm)-15,000K (cold) and chose 8 scents (4 warm scents, 4 cold scents) which showed clear correspondence with warm or cold color-temperatures (Expt. 1). And then after applying warm (3,000K), neutral (6,500K), or cold (14,000K) color-temperatures to images or videos, we presented warm or cold scents to participants while they rate reality improvement on a 7-point scale depending on relatedness of scent vs. color-temperature (related, unrelated, neutral) (Expts. 2-3). The results showed that participants experienced greater reality when scent and color-temperature was related than when they were unrelated or neutral. This research has important practical implications in demonstrating the possibility that provision of color-temperature related scent improves video reality even when there are no concrete objects that suggest specific olfactory information.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.590-597
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• 2013

Principle component and emission localization of volatile compounds were investigated according to scent intensity of rose flower. Scent intensity in cultivars and bred-line of Rosa hybrida was divided into three levels; light ('Feel Lip', 'Venus Berry'), medium ('GR07-135'), strong ('Honey Blue'). The major volatile compounds were different depending on the cultivars and selected line; 3,5-dimethoxytoluene (DMT), benzene, 1,3,5-trimethoxy ('Feel Lip'), megastigma-4,6(Z),8(E)-triene ('Venus Berry'), DMT, benzene,1-ethenyl-4-methoxyand, phenylethylalcohol ('GR07-135') and germacrene-D, DMT ('Honey Blue'). The adaxial epidermal cells were conical papillate shape, whereas the abaxial epidermal cells were flat shape. The adaxial epidermal cells of 3 cultivars and 1 selected line were surrounded by thick cell wall and covered by waxy cuticle of 2 cultivars and 1 selected line (except 'Honey Blue'). The adaxial epidermal cells contained starches in 'Feel Lip', osmiophlic droplets in 'Venus Berry', starchs, plastids, vacuoles in 'GR07-135' and plastoglobules, plastids, vacuoles in 'Honey Blue'. Based on these results, it appears that plastids and vacuoles in adaxial epidermal cells with conical papillate shape are associated production and emission of volatile compounds in scent R. hybrida.

• Journal of Ecology and Environment
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• v.46 no.1
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• pp.62-75
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• 2022

Background: Pollinators help plants to reproduce and support economically valuable food for humans and entire ecosystems. However, declines of pollinators along with population growth and increasing agricultural activities hamper this mutual interaction. Nectar and pollen are the major reward for pollinators and flower morphology and volatiles mediate the specialized plant-pollinator interactions. Limited information is available on the volatile profiles attractive to honey bees and bumblebees. In this study we analyzed the volatile organic compounds of the flowers of 9 different plant species that are predominantly visited by honey bees and bumblebees. The chemical compositions of the volatiles were determined using a head space gas chromatography-mass spectrometry (GC-MS) method, designed to understand the plant-pollinator chemical interaction. Results: Results showed the monoterpene 1,3,6-octatriene, 3,7-dimethyl-, (E) (E-𝞫-ocimene) was the dominating compound in most flowers analyzed, e.g., in proportion of 60.3% in Lonicera japonica, 48.8% in Diospyros lotus, 38.4% Amorpha fruticosa and 23.7% in Robinia pseudoacacia. Ailanthus altissima exhibited other monoterpenes such as 3,7-dimethyl-1,6-octadien-3-ol (𝞫-linalool) (39.1%) and (5E)-3,5-dimethylocta-1,5,7-trien-3-ol (hotrienol) (32.1%) as predominant compounds. Nitrogen containing volatile organic compounds (VOCs) were occurring principally in Corydalis speciosa; 1H-pyrrole, 2,3-dimethyl- (50.0%) and pyrimidine, 2-methyl- (40.2%), and in Diospyros kaki; 1-triazene, 3,3-dimethyl-1-phenyl (40.5%). Ligustrum obtusifolium flower scent contains isopropoxycarbamic acid, ethyl ester (21.1%) and n-octane (13.4%) as major compounds. In Castanea crenata the preeminent compound is 1-phenylethanone (acetophenone) (46.7%). Conclusions: Olfactory cues are important for pollinators to locate their floral resources. Based on our results we conclude monoterpenes might be used as major chemical mediators attractive to both honey bees and bumblebees to their host flowers. However, the mode of action of these chemicals and possible synergistic effects for olfaction need further investigation.

• FLOWER RESEARCH JOURNAL
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• v.26 no.4
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• pp.216-220
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• 2018

Freesia (Freesia hybrida Hort.) 'Sweet Lemon' was developed for the cut flowers in National Institute of Horticultural Herbal Science, Rural Development Administration, Korea in 2015. This hybrid was crossed and selected from 'Teresa' and 'Yvonne' in 2007. Morphological characteristics of the selected freesia hybrid were investigated for 5 years from 2009 to 2014, and then it was named 'Sweet Lemon' in 2015. 'Sweet Lemon' has double and lemon petals (RHS color chart Y2B). The average flower diameter was 6.8 cm and the average yield is 6.7. The growth of the plant is vigorous and the average plant height is 99.0cm, which is 34.7cm higher than the standard cultivar 'Yvonne'. The average floret number per stalk of 'Sweet Lemon' was 10.7, and stalk length was 7.7 cm. The average days to first flowering of 'Sweet Lemon', 126 days, was approximately 20 days earlier than the control cultivar. It's average vase life and yield is 8.7 days and 5.6 cormlets per plant, respectively. Totally 58 of volatile compounds were identified using gas chromatography-mass spectrometry (GC-MS), the major components were linalool, alpha-terpineol, alpha-selinene, and limonene.