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Scent Analysis Using an Electronic Nose and Flowering Period of Potted Diploid and Tetraploid Cymbidium

심비디움 2배체, 4배체의 분화수명 조사 및 전자코를 이용한 향기패턴분석

  • Hwang, Sook-Hyun (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Mi-Seon (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Pue-Hee (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, So-Young (Department of Horticultural Science, Chungbuk National University)
  • 황숙현 (국립원예특작과학원 화훼과) ;
  • 김미선 (국립원예특작과학원 화훼과) ;
  • 박부희 (국립원예특작과학원 화훼과) ;
  • 박소영 (충북대학교 축산.원예.식품공학부)
  • Received : 2015.03.31
  • Accepted : 2015.08.14
  • Published : 2016.02.29

Abstract

We investigated the intensity and pattern of the scent produced by diploid and tetraploid Cymbidium flowers, using an electronic nose with 6 metal oxide sensors (MOS). The MOS responses were evaluated by principal component analysis, discriminant function analysis, and sensor data. These analyses revealed that tetraploid flowers had a stronger scent than diploid flowers in Cymbidium Golden Elf 'Sundust'. Furthermore, among the different flower parts-column, lip, and petals-the column produced the strongest scent. There was no significant difference between the flowering periods of diploid and tetraploid potted Cymbidium Golden Elf 'Sundust' and Cymbidium Elma 'Orient Toyo' grown in a greenhouse. Moreover, there were no significant differences between the number of flowers per flower stem and the length of flower stems on the diploid and tetraploid plants of these two Cymbidium cultivars. This study provides potentially useful information for the breeding of polyploidy Cymbidium in the floriculture industry.

배수성에 따른 심비디움 꽃 향기의 변화를 알아보기 위해 전자코를 이용하여 C. Golden Elf 'Sundust'의 2배체와 4배체 꽃을 PCA(주성분분석)와 DFA(판별함수분석)로 분석한 결과, 꽃 한 송이의 향기는 2배체에 비해 4배체에서 강하게 나타났고, 화기 조직별 향기는 예주가 가장 강하게 나타났으며 꽃잎과 설판은 근접한 패턴을 보였다. 배수성에 따른 심비디움의 분화수명의 차이를 알아보기 위해 C. Golden Elf 'Sundust'와 C. Elma 'Orient Toyo' 분화의 화경을 조사한 결과 개화기간뿐 만 아니라 화경당 화수, 화경장에서 모두 차이가 나타나지 않았다. 본 연구는 심비디움을 비롯한 다른 종류의 난과식물 배수체 육종에 있어 기초자료로 유용하게 활용 될 것이다.

Keywords

References

  1. Alpha M.O.S. 1998. Intelligent electronic nose. FOX user manual. 2nd ed. SA, France. 1:25-31.
  2. Byun, M.S., M.J. Kim, M.K. Kwon, Y.Y. Han, and K.W. Kim. 2007. Characteristic comparison of the flower scent in different flowering stages and floral organs of Lilium using an electronic nose. Flower Res. J. 15:1-8.
  3. Dudareva, N. and E. Pichersky. 2006. Biology of floral scent, Taylor & Francis Group. Boca Raton. FL, USA. p. 346.
  4. Gardner, H.W., D.L., Dornbos Jr., and A.E., Desjardins. 1990. Hexanal, trans-2-hexenal, and trans-2-nonenal inhibit soybean, Glycine max, seed germination. J. Agric. Food Chem. 38:1316-1320. https://doi.org/10.1021/jf00096a005
  5. Gross, K. and F.P. Schiestl. 2015. Are tetraploids more successful? Floral signals, reproductive success and floral isolation in mixed-ploidy populations of a terrestrial orchid. Ann. Bot. 15: 263-273.
  6. Guterman, I., S.M. Menda, D. Piestun, M. Dafny-Yelin, G. Shalev, E. Bar, O. Davydov, M. Ovadis, M. Emanuel, J. Wang, Z. Adam, E. Pichersky, E. Lewinsohn, D. Zamir, A. Vainstein, and D. Weiss. 2002. Rose scent: genomics approach to discovering novel floral fragrance–related genes. Plant Cell 14:2325-2338. https://doi.org/10.1105/tpc.005207
  7. Inoue, K., T. Kato, A. Nobukuni, H. Kunitake, and T. Yabuya. 2006. Characterization of tetraploid plants regenerated via protoplast culture of Iris fulva and their crossability with Japanese irises. Sci. Hortic. 10:334-339
  8. Kim, H.S. 2006. Effects of the floral arts program on psychology and brain EEG of middle - aged women. J. Korean Floral Art Res. Assn. 8:56-69.
  9. Kim, M.S., J.Y. Won, Y.R. Lee, J.Y. Kim, B.H. Kim, and J.S. Eun. 1998. Induction of polyploidy in vitro of Cymbidium by colchicine treatment. Korean J. Hortic. Sci. Technol. 16: 115. (Abstr.)
  10. Kim, M.S., J.Y. Kim, and J.S. Eun. 2003. Chromosome doubling of a Cymbidium hybrid with colchicine treatment in meristem culture. Proc. Nagoya Intl. Orchid Congr. Nagoya, Japan. p. 37-40.
  11. Kim, M.S., Y.R. Lee, and J.S. Eun. 2006. Induction of polyploidy of Cymbidium Golden Elf 'Sundust' by colchicine treatment. 27th Intl. Hortic. Cong. Seoul, Korea. p. 157. (Abstr.)
  12. Kim, M.S., J.Y. Kim, and J.S. Eun. 2008. Duplication of the chromosome number of diploid Cymbidium Silky 'Wind' using colchicines. Proc. 1st Asian Hortic. Cong. Jeju, Korea. p. 110-111. (Abstr.)
  13. Lee, K.H., P.H. Park, B.W. Yea, Y.R. Lee, and P.M. Park. 2012. Analysis of flower tissues, collection time, and flowering day in fragrant Cymbidium 'Hwanhee' by electronic nose. Korean J. Hortic. Sci. Technol. 30:154. (Abstr.)
  14. Lee, Y.R., J.W. Chung, and S.R. Kim. 2003. Analysis of fragrance patterns of Rosa hybrida using electronic nose system. Flower Res. J. 11:1-4.
  15. Lee, Y.R., S.R. Choi, B.W. Yae, and D.S. Lee. 2012 Analysis of cut flowers vase life to pot Cymbidium cultivars. Flower Res. J. 20:40-44
  16. Liu, S., S. Chen, Y. Chen, Z. Guan, D. Yin, and F. Chen. 2011. In vitro induced tetraploid of Dendranthema nankingense (Nakai) Tzvel. shows an improved level of abiotic stress tolerance. Sci. Hortic. 27:411-419.
  17. Menninger, E.D. 1963. Diary of a colchicine-induced tetraploid Cymbidium. Am. Orchid Soc. Bull. 32:885-887.
  18. MAF. 2013. Flower cultivation status. (http://www.mafra.go.kr)
  19. Noh, B.S., J.W. Ko, S.Y. Kim, and S.J. Kim. 1998. Application of electronic nose in discrimination of the habitat for special agricultural products. Korean J. Food Sci. Technol. 30:1051-1057.
  20. Park, B.H., B.W. Yae, M.S. Kim, Y.R. Lee, P.M. Park, and D.S. Lee. 2011. Fragrance analysis using GC-MS and electronic nose in Phalaenopsis. Flower Res. J. 19:219-224.
  21. Park, H. 1999. Expression mechanism and bioactivity of flower fragrance. Flower Color Fragrance 1:49-68.
  22. Ranney, T.G. 2006. Polyploidy: from evolution to new plant development. Proc. Intl. Plant Prop. Soc. 56:137-142.
  23. Shin, J.A., J.H. Gwon, and K.T. Lee. 2003. Aroma analysis by the electronic nose on red ginseng powder treated with gamma radiation, methyl bromide and phosphine. Korean J. Food Sci. Technol. 35:825-829.
  24. Staples, E.J. 1999. Electronic nose simulation of olfactory response containing 500 orthogonal sensors in 10 seconds. Proc. 1999 IEEE Ultrasonics frequency control and ferroelectrics symp. Lake tahoe, CA. p. 417-423.
  25. Tang, Z.Q., D.L. Chen, Z.J. Song, Y.C. He, and D.T. Cai. 2010. In vitro induction and identification of tetraploid plants of Paulownia tomentosa. Plant Cell Tissue Organ Cult. 102:213-220. https://doi.org/10.1007/s11240-010-9724-6
  26. Yae, B.W., I.K. Lee, M.S. Kim, P.H. Park, Y.R. Lee, B.N. Jung, K.S. Han, T.J. Kang, and D.S. Lee. 2011. Standard farming handbook: Cymbidium. Rural Development Administration. Suwon. p. 9-185.
  27. Wimber, D.E. and A. Van Cott. 1966. Artificially induced polyploidy in Cymbidiums. Proc. 5th World Orchid Conf. California, USA. p. 27-31.
  28. Wimber, D.E., S. Watrous, and A.J. Mollahan. 1987. Colchicine induced polyploidy in orchids. Proc. 5th World Orchid Conf. California, USA. p. 65-69.