• Journal of The Korean Society of Agricultural Engineers
• /
• v.47 no.3
• /
• pp.77-86
• /
• 2005

A monitoring study has been conducted to identify hydrologic conditions, water quality and nutrient loading characteristics of small watershed in Juam Lake. Climate data of the watershed were collected; flow rate was measured and water quality sampling was conducted at the watershed outlet for this study. Water quality data revealed that T-P concentrations meet I grade of lake water quality standard during non-storm period, but degraded up to II-III grade of lake water quality standard during storm period. The observed T-N concentrations always exceeded lake water quality standard. Therefore, T-P was identified as limiting chemical constituent for eutrophication of Juam Lake. T-P concentration of non-storm period also revealed that point source pollution is not serious in the watershed. Three year monitoring results showed that the observed T-N losses were $10.85\~18.88$ kg/ha and T-P losses were $0.028\~0.323$ kg/ha during six month (Mar. - Oct.), respectively. Major portion of runoff amount discharged by a few storm events a year and nutrient load showed apparent seasonal variation. Huge runoff amounts were generated by intense storms, which make application of water treatment or detention facilities ineffective. Monitoring results confirmed that water quality improvement by abating nonpoint source pollution in rural watershed of monsoon climate should be focused on source control. T-P losses from paddy field seemed to consist of significant amount of total load from study watershed. Therefore, management of drainage from paddy field is considered to be important for preventing algal blooming problem in Juam Lake.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.12
• /
• pp.2135-2145
• /
• 2015

VP7, an outer capsid protein of grass carp reovirus (GCRV), was expressed and displayed on the surface of Saccharomyces cerevisiae for developing an efficient vaccine against hemorrhagic disease of grass carp. The result of flow cytometry analysis indicated that protein VP7 could be displayed on the surface of yeast cells after inducing with galactose. The expression of VP7 was confirmed by western blot analysis and further visualized with confocal microscopy. The specific antibodies against VP7 generated from mice were detectable from all immune groups except the control group, which was immunized with untransformed yeast cells. The displaying VP7 on glycosylation-deficient strain EBYΔMnn9 was detected to induce a relatively low level of specific antibody amongst the three strains. However, the antiserum of EBYΔM9-VP7 showed relative high capacity to neutralize GCRV. Further neutralization testing assays indicated that the neutralizing ability of antiserum of the EBYΔM9-VP7 group appeared concentration dependent, and could be up to 66.7% when the antiserum was diluted to 1:50. This result indicates that appropriate gene modification of glycosylation in a yeast strain has essential effect on the immunogenicity of a yeast-based vaccine.

• Journal of Microbiology and Biotechnology
• /
• v.27 no.6
• /
• pp.1098-1105
• /
• 2017

Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral, and artificial viral vectors. The objective of this study was to establish a potential approach for large-scale production of VSV-G. To this end, VSV-G was cloned with an N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones ($Mut^s$) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, whereas the proliferation was reduced significantly under induction conditions. VSV-G protein was detected in cell lysates by western blot analysis, and the highest expression level was observed at 96 h post induction. VSV-G could also be obtained from the condition medium of yeast protoplasts. Furthermore, VSV-G could be incorporated into Ad293 cells and was able to induce cell fusion, leading to the transfer of cytoplasmic protein. Finally, VSV-G-mediated DNA transfection was assayed by flow cytometry and luciferase measurement. Incubation of VSV-G lysate with the pGL3-control DNA complex increased the luciferase activity in Ad293 and HeLa cells by about 3-fold. Likewise, incubation of VSV-G lysate with the pCMV-DsRed DNA complex improved the transfection efficiency into Ad293 by 10% and into HeLa cells by about 1-fold. In conclusion, these results demonstrate that VSV-G could be produced from P. pastoris with biofunctionalities, demonstrating that large-scale production of the viral glycoprotein is feasible.

• The Korean Journal of Physiology and Pharmacology
• /
• v.21 no.5
• /
• pp.465-474
• /
• 2017

The aim of this study was to determine the raising anticancer effects of resveratrol (Res) on paclitaxel (PA) in non-small cell lung cancer (NSCLC) cell line A549. The $10{\mu}g/ml$ of Res had no effect on human fetal lung fibroblast MRC-5 cells or on A549 cancer cells and the 5 or $10{\mu}g/ml$ of PA also had no effect on MRC-5 normal cells. PA-L ($5{\mu}g/ml$) and PA-H ($10{\mu}g/ml$) had the growth inhibitory effects in NSCLC cell line A549, and Res increased these growth inhibitory effects. By flow cytometry experiment, after Res ($5{\mu}g/ml$)+PA-H ($10{\mu}g/ml$) treatment, the A549 cells showed the most apoptosic cells compared to other group treatments, and after additional treatment with Res, the apoptosic cells of both two PA concentrations were raised. Res+PA could reduce the mRNA and protein expressions of COX-2, and Res+PA could reduce the COX-2 related genes of VEGF, MMP-1, MMP-2, MMP-9, $NF-{\kappa}B$, Bcl-2, BclxL, procollagen I, collagen I, collagen III and CTGF, $TNF-{\alpha}$, $IL-1{\beta}$, iNOS and raise the TIMP-1, TIMP-2, TIMP-3, $I{\kappa}B-{\alpha}$, p53, p21, caspase-3, caspase-8, caspase-9, Bax genes compared to the control cells and the PA treated cells. From these results, it can be suggested that Res could raise the anticancer effects of PA in A549 cells, thus Res might be used as a good sensitizing agent for PA.

• Korean Journal of Cognitive Science
• /
• v.12 no.1_2
• /
• pp.55-63
• /
• 2001

Speaking generally, agent-based technology is a kind of technology to handle the flood of information resulted from the popularization of the internet. Agent system is a multi-distributed system which consists of both homogeneous and heterogeneous agents. Generally there is a coordination agent in between which is in charge of control and m message flow among the application agents. The purpose of this thesis is to propose a coordination method among agents, some of which provide informations and some of which request them. In multi-agent system, the Information Providing Agent(IPA) registers its capabilities to Coordination Agent(CA) and the Information Requesting Agent(lRA) requests CA what it needs. To coordinate them with satisfactory results the coordination agent ought to have an ability to return a relatively proper data to the requester which is supposed to be similar even though it is not so exact as was intended. For this, this thesis proposes a scheme for an coordination agent to find an IPA which provides an information which correlates most closely with that of IRA.

• Journal of Korea Multimedia Society
• /
• v.13 no.2
• /
• pp.279-286
• /
• 2010

Unbalanced traffic load offered to the nodes making up a path in the network guaranteeing quality-of-service has been known as a main cause deteriorating the capability of the path in admitting traffic flows. Several path load balancing methods have been developed to resolve this problem which used a feedback control scheme that adjusts the delay budget of a flow allocated to each node according to the conditions of available resource in the path. Because of no consideration about the probabilistic characteristics of the service quality, it is impossible for them to prevent in advance a bottleneck on the path which leads to a native restriction in the improvement of the capability being deteriorated. This paper proposes a preventive intra-path load balancing method applicable to the RSVP system which is based on the probabilistic characteristics of the quality-of-service of the offered load. The results of the simulation of the proposed method on a simple evaluation network showed that it provides the gain of 4~22% compared to the legacy one in terms of the number of admitted flows.

• Journal of Korea Multimedia Society
• /
• v.13 no.2
• /
• pp.308-315
• /
• 2010

User Generated contents(UGC) interchange with internet users actively in Web2.0 environment. According to growth of content sharing site, the number of non-expert's contents increased. But non-expert's contents have a simple media just recorded. For providing actual feeling like effects and actions to non-expert's contents, we suggest Fusion Media Middleware(FMM). The FMM can increase user satisfaction by providing actual feeling. Furthermore, The content changes advanced media that has emotional impression. The FMM for providing actual feeling classify the inputted media as a scene based on MPEG-7. The FMM provide an actual feeling to simple media by inserting effects like a sound, image and text among the classified media. Using the BSD code of MPEG-21, the FMM can link up with inputted media and effects. Through the mapping BSD code the FMM control synchronization between media and effects. In this paper, Using the Fusion Media Middleware, the non-expert's contents express value as multimedia that has an actual feeling. Futhermore, the FMM creates flow of new media circulation.

• Journal of KIISE:Information Networking
• /
• v.27 no.1
• /
• pp.98-107
• /
• 2000

This paper compares the performance of feedback consolidation algorithms with or without a fast overload indication function which has been recently proposed in the ATM Forum for multicast ABR (Available Bit Rate) services in ATM networks. We use the performance criteria such as the ACR (Allowed Cell Rate) of a source, queue length at a switch, link utilization, fairness, and BRM (Backward Resource Management) cell overhead. Simulation results show that performance of the feedback consolidation algorithms with a fast overload indication function can be improved sign.ificantly than that of algorithms without this function in terms of ramp-down delay, particularly in initial start-up period or under a severe overload situation. The fast overload indication function can be combined with any feedback consolidation algorithm, whereas its performance is highly dependent on an underlying basic feedback consolidation algorithm.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.20 no.3
• /
• pp.549-557
• /
• 2016

It is important for effective visualization to summarize not only a large amount of debugging information but also the mental models of abstract ideas. This paper presents an abstract visualization tool which provides effective visualization of thread structure and race information for OpenMP programs with critical sections and nested parallelism, using a partial order execution graph which captures logical concurrency among threads. This tool is supported by an on-the-fly trace-filtering technique to reduce space complexity of visualization information, and a graph abstraction technique to reduce visual complexity of nested parallelism and critical sections in the filtered trace. The graph abstraction of partial-order relation and race information is effective for understanding program execution and detecting to eliminate races, because the user can examine control flow of program and locations of races in a structural fashion.

• Journal of the Korean Society of Hazard Mitigation
• /
• v.10 no.4
• /
• pp.81-88
• /
• 2010

As there occurs recently and frequently a flash flood due to the climate change, a sudden local flood of great volume and short duration caused by heavy or excessive rainfall in a short period of time over a small area, it is increasing that significant danger and loss of life and property in Korea as well as the whole world. Since a flash flood usually occurs as the result of intense rainfall over small steep slope regions and has rapid runoff and debris flow, a flood rises quite quickly with little or no advance warning to prevent flood damage. The aim of this study is to quantify the severity of flash food by estimation of a flash flood index(FFI) from probability rainfall data in a study basin. FFI-D-F(FFI-Duration-Frequency) curves that present the relative severity of flash flood are developed for a study basin to provide regional basic information for the local flood forecasting and warning system particularly in ungauged catchments. It is also expected that FFI-D-F curves can be utilized for evaluation on flash flood mitigation ability and residual flood risk of both existing and planned flood control facilities.