• Natural Product Sciences
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• 제20권3호
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• pp.170-175
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• 2014

Twenty five methanolic plant extracts were investigated to determine the anticancer activity against sonic hedgehog (shh)/Gli signaling pathway dependent cancer, PANC-1 human pancreatic cancer cells, through three screening programs. All extracts were inspected their inhibitory properties on sonic hedgehog-conditioned medium (shh-CM) induced alkaline phosphatase (ALP) activity in C3H10T1/2 mouse mesenchymal stem cells to examine whether the plant extracts affect the shh/Gli signaling pathway. Next, plant extracts were screened the ability to suppress the cell proliferation of PANC-1 human pancreatic cancer cells. Finally, active plant extracts from the two screening systems were evaluated for the suppressive effect on Gli-mediated transcriptional activity in PANC-1 cells. Among active plants, Cinnamomum cassia suppressed Gli-mediated transcriptional activity leading to the down-regulated expression of Gli-target genes such as Gli-1 and Patched-1 (Ptch-1). This study provides the consideration for the important role of natural products in drug discovery process as well as the basis for the further analysis of active plant and potential identification of novel bioactive compounds as inhibitors of Gli and therapeutic candidates against shh/Gli signaling pathway dependent cancers.

• The Plant Pathology Journal
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• 제38권6호
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• pp.685-691
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• 2022

Plants produce chemicals of immense diversity that provide great opportunities for development of new antifungal compounds. In search for environment-friendly alternatives to the fungicide of current use, we screened plant extracts obtained from more than eight hundred plant materials collected in Korea for their antifungal activity against the model plant pathogenic fungus, Magnaporthe oryzae. This initial screening identified antifungal activities from the eleven plant extract samples, among which nine showed reproducibility in the follow-up screening. These nine samples were able to suppress not only M. oryzae but also other fungal pathogens. Interestingly, the plant extracts obtained from Actinostemma lobatum comprised five out of eight samples, and were the most effective in their antifungal activity. We found that butanol fraction of the A. lobatum extract is the most potent. Identification and characterization of antifungal substances in the A. lobatum extracts would provide the promising lead compounds for new fungicide.

• 생약학회지
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• 제46권1호
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• pp.84-92
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• 2015

Tyrosinase is a key enzyme to control the biosynthesis of melanin pigments and has two enzyme activities, namely of 1-tyrosine hydroxylase and of 1-dopa oxidase. Thus, tyrosinase is regarded as a target in skin-whitening and therapeutic intervention of local hyperpigmentation diseases. We have tested tyrosinase inhibitory activity on the water extracts of 50 species oriental medicinal plant. Among them, five medicinal plants, Linderae Radix, Clematidis Radix, Cinnamomi Cortex Spissus, Fritillariae Thunbergii Bulbus and Bulbus Fritillariae Cirrhosae were investigated strong inhibition effect. Five medicinal plants were fractionated using organic solvents (methylene chloride, ethyl acetate, n-butanol, water). Cinnamomi Cortex Spissus (ethyl acetate fraction) was investigated strong inhibition effect. Tyrosinase inhibitory activity below $IC_{50}\;40{\mu}g/ml$ is confirmed in five herbal plants that are Linderae Radix, Clematidis Radix, Cinnamomi Cortex Spissus, Fritillariae Thunbergii Bulbus and Bulbus Fritillariae Cirrhosae. Tyrosinase inhibitory levels ($IC_{50}\;{\mu}g/ml$) of each plants were 15.56, 35.02, 25.14, 15.20 and 39.77. We also investigate the effect of effective plant's fraction. in dose of $100{\mu}g/ml$, Cinnamomi Cortex Spissus (P-36) EtOAc fraction significant inhibitory effect over 50%. Clematidis Radix (P-35) and Cinnamomi Cortex Spissus (P-36) MC fraction inhibit tyrosinase each 36.60% and 43.21%. inhibitory rates of Fritillariae Thunbergii Bulbus (P-40) EtOAc and $H_2O$ fraction are 31.40% and 31.51%. Bulbus Fritillariae Cirrhosae (P-45) BuOH fraction regulate tyrosinase activity to 37.71%. We examined tyrosinase inhibitory activity of natural products and these results suggest that several herbs have potential as a new whitening material.

• Natural Product Sciences
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• 제19권2호
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• pp.192-200
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• 2013

As part of the research for the natural products about prostate-related disease, this study screened 159 plant species from 46 families, which included a total of 213 different kinds of local native plants and these plants were tested for the ability to inhibit LNCaP proliferation, an androgen-sensitive prostate cancer cell line, and DU145 proliferation, which is a more aggressive androgen-insensitive prostate cancer cell line. The results indicated that nineteen of 213 types of plants exhibited antiproliferative activity (cell viability < 30%, $500{\mu}g/mL$) on the growth of androgen-sensitive LNCaP cell lines, and five of them exhibited DU145 cell antiproliferative activity (cell viability < 30%, $500{\mu}g/mL$). The methanol extracts of Eurya emarginata (stems), Gleditsia japonica var. koraiensis (leaves), Photinia glabra (leaves) and Elaeagnus macrophylla (leaves) showed antiproliferative activity on both the androgen-sensitive LNCaP cells (cell viability < 30%) and androgen-insensitive DU145 cells (cell viability > 100%). The study also found that the methanol extracts of Styrax japonica (fruits), Aralia continentalis (leaves), Fagus crenata var. multinervis (stems), Thuja orientalis (stems) and Poncirus trifoliate (branches) presented the strongest activity and demonstrated potent antiproliferative activity on both cell lines (LNCaP and DU145 cell viability < 30%).

• 한국응용곤충학회지
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• 제61권2호
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• pp.369-376
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• 2022

본 연구에서는, 5가지 식물의 메탄올 추출물을 이용하여 모기기피 효과 실험을 진행하고 누드 쥐를 모델로 하여 흰줄숲모기에 대한 모기기피제의 유효용량 및 유효시간을 평가하였다. 그리고 식물의 휘발성분을 GC-MS로 분석하였다. 유효용량은 개박하가 10, 50, 100, 200 ㎍/mL에서 72.9, 83.7, 86.4, 97.3%의 효율로 가장 높았다. 누드 쥐를 대상으로 한 유효시간 연구에서는 5가지 식물의 메탄올 추출물 중 개박하, 개다래 및 개똥쑥이 90.5%로, 30분까지만 지속하였다. 각 식물의 휘발성분을 분석한 결과, 애플민트 28개 성분, 개다래 19개 성분, 개똥쑥 27개 성분, 개박하 26개 성분, 민들레 19개 성분이 확인 되었다. 특히 모기기피제의 유효 성분으로 알려진 네페탈락톤(nepetalactone)이 개박하의 휘발성분 중 한 성분으로 확인 되었다(27.95 mg/Kg). 본 연구결과는 다양한 휘발성분을 함유한 식물을 이용한 모기기피제 재료로서 가능성을 제시한다.

• Toxicological Research
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• 제26권4호
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• pp.321-327
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• 2010

The ability of 80% ethanol extracts from five medicinal plants, Aralia continentalis, Paeonia suffruticosa, Magnolia denudata, Anemarrhena asphodeloides, and Schizonepeta tenuifolia, to neutralize hydroxyl radical, peroxyl radical and peroxynitrite was examined using the total oxyradical scavenging capacity (TOSC) assay. Peroxyl radical was generated from thermal homolysis of 2,2'-azobis(2-methylpropionamidine) dihydrochloride (ABAP); hydroxyl radical by an iron-ascorbate Fenton reaction; peroxynitrite by spontaneous decomposition of 3-morpholinosydnonimine N-ethylcarbamide (SIN-1). The oxidants generated react with $\alpha$-keto-$\gamma$-methiolbutyric acid (KMBA) to yield ethylene, and the TOSC of the substances tested is quantified from their ability to inhibit ethylene formation. Extracts from P. suffruticosa, M. denudata, and S. tenuifolia were determined to be potent peroxyl radical scavenging agents with a specific TOSC (sTOSC) being at least six-fold greater than that of glutathione (GSH). These three plants also showed sTOSCs toward peroxynitrite markedly greater than sTOSC of GSH, however, only P. suffruticosa revealed a significant hydroxyl radical scavenging capacity. Seven major active constituents isolated from P. suffruticosa, quercetin, (+)-catechin, methyl gallate, gallic acid, benzoic acid, benzoyl paeoniflorin and paeoniflorin, were determined for their antioxidant potential toward peroxynitrite, peroxyl and hydroxyl radicals. Quercetin, (+)-catechin, methyl gallate, and gallic acid exhibited sTOSCs 40~85 times greater than sTOSC of GSH. These four components also showed a peroxynitrite scavenging capacity higher than at least 10-fold of GSH. For antioxidant activity against hydroxyl radical, methyl gallate was greatest followed by gallic acid and quercetin. Further studies need to be conducted to substantiate the significance of scavenging a specific oxidant in the prevention of cellular injury and disease states caused by the reactive free radical species.

• 한국환경농학회지
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• 제5권2호
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• pp.85-94
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• 1986

수도체(水稻體)와 토양(土壤)에서 carbofuran의 거취(去就)를 구명(究明)하기 위하여 C-14 표식(標識) carbofuran을 수도유묘(水稻幼苗)에 담수처리(湛水處理)하고 방사능(放射能)의 분포(分布), 수도체내(水稻體內)에서의 대사(代謝), 토양(土壤)에서의 화학적 전환(化學的 轉換)을 경시적(經時的)으로 조사(調査)하였다. 토양(土壤)에 처리(處理)한 C-14 표식(標識) carbofuran은 쉽게 경근흡수(經根吸收)되어 줄기로 이동(移動), 잎의 첨단부위(尖端部位)에 축적(蓄積)되었으며, 수도체(水稻體)의 근부(根部)에 비해 더 많은 양(量)의 방사능(放射能)이 줄기에 분배(分配)되었다. 수도(水稻) 줄기와 뿌리에서 aqueous phase extracts와 non-extractable fraction의 방사능(放射能)이 경제적(經時的)으로 증가(增加)하는 것은 phase I 및 phase II 반응(反應)이 진행(進行)하는 것으로 해석(解釋)하였다. 수도체(水稻體)와 토양(土壤)의 organic phase extracts에는 현재(現在)까지 알려진 carbofuran과 이의 5종(種) 대사물(代謝物)이 모두 관찰(觀察)되었으며 3-hydroxycarbofuran이 주요(主要) 대사물(代謝物)로 확인(確認)되었다. aqueous phase extracts는 HCl 또는 cellulase 가수분해(加水分解)로 상기(上記)한 6종(種) 화합물(化合物)이 양적차이(量的 差異)는 있었으나, 모두 aglycone으로 확인(確認)되었으며 7-benzofuranol과 3-hydroxycarbofuran이 많이 분리(分離)되었다. 일반토양(一般土壤)에서는 살균토양(殺菌土壤)에 비해 non-extractable fraction의 방사능(放射能)이 크게 증가(增加)하였고, organic phase extrac 방사능(t放射能)은 감소(減少)하였다.

• 생약학회지
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• 제25권4호통권99호
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• pp.382-387
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• 1994

Antineoplastic activity against human gastric and colon carcinoma cell lines was measured in 49 extracts from 46 plants using MTT (3-[4, 5-dimethyl thiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) method. Six extracts from five plants have been reported to have antineoplastic effect. Extracts from remaining 41 plants failed to show significant cytotoxic effect at the concentration of less than $230\;{\mu}g/ml$.

• 생태와환경
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• 제33권2호통권90호
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• pp.136-144
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• 2000

팔당호 주변에서 자생하는 식물체를 이용하여 실험실 내에서 배양된 남조류에 투여하여 조류증식 억제 효율을 조사하였다. 조류 성장 억제 효과에 있어 육상식물을 이용하는 경우 조류 밀도가 10$^{4}$ cells/ml 범위에서의 0.5g/l 투여량은 전혀 효과를 나타내지 못했던 반면, 2.5 g/l 투여량에서는 95% 이상의 효과를 보였다. 조류 밀도가 10$^{6}$ cells/ml 범위에서의 육상식물 투여량은 2.5 g/l 이상이 필요했다. 조류성장 억제에 육상식물은 잣잎, 수생식물은 애기마름이 큰 효과를 보였다. 애기마름 투여조는 조류 밀도가 10$^{6}$ cells/ml 범위에서도 2.5 g/l 투여량으로 50%의 억제 효율이 나타났다. 식물을 이용한 조류 증식 억제 효과는 식물 투여 후 7일째 후 가장 효과가 좋은 것으로 나타났다. 습체, 건체, 추출물 비교시 추출물이 가장 효과가 좋은 것으로 육상식물 투여조의 경우 1.25 g/l 투여량에 비해 2.5 g/l 투여량에서 현저한 효과를 보였고, 애기마름은 1.25 g/l 투여량에서도 99%의 효과를 보였다. 건체식물은 습체식물에 비해 초기에도 높은 효과를 보였다. 조류의 비증식속도로 보았을 때도 식물체 추출물을 투여했을 때 가장 효과가 좋았고 다음으로 건체, 습체식물 투여순으로 조류 증식억제 효과를 나타내었다.

• 생약학회지
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• 제27권4호
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• pp.383-388
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• 1996

The objective of this reseach is to find new antitumoral substances from natural products. Several of natural products have been used as food that were isolated into hexane(Hex.) and/or ethylacetate(EtOAc) extracts. we have tested cytotoxicities of these plants against human solid tumor cells. The cytotoxic activity of these plants were tested using Sulforhodamin B(SRB) assay. Hexane extracts of Chrysanthemum sinense, Allium tubersum. Beta vulgaris, Ixeris dentata have revealed cytotoxicities against five human solid tumor cells, and its cytotoxicities of each cell line were $10-100\;{\mu}l/ml$ ED50 (Effective dose that cause 50% inhibition of cell growth in vitro)