• 한국발생생물학회지:발생과생식
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• 제26권1호
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• pp.23-36
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• 2022

Pharyngeal pouches, a series of outgrowths of the pharyngeal endoderm, are a key epithelial structure governing facial skeleton development in vertebrates. Pouch formation is achieved through collective cell migration and rearrangement of pouch-forming cells controlled by actin cytoskeleton dynamics. While essential transcription factors and signaling molecules have been identified in pouch formation, regulators of actin cytoskeleton dynamics have not been reported yet in any vertebrates. Cofilin1-like (Cfl1l) is a fish-specific member of the Actin-depolymerizing factor (ADF)/Cofilin family, a critical regulator of actin cytoskeleton dynamics in eukaryotic cells. Here, we report the expression and function of cfl1l in pouch development in zebrafish. We first showed that fish cfl1l might be an ortholog of vertebrate adf, based on phylogenetic analysis of vertebrate adf and cfl genes. During pouch formation, cfl1l was expressed sequentially in the developing pouches but not in the posterior cell mass in which future pouch-forming cells are present. However, pouches, as well as facial cartilages whose development is dependent upon pouch formation, were unaffected by loss-of-function mutations in cfl1l. Although it could not be completely ruled out a possibility of a genetic redundancy of Cfl1l with other Cfls, our results suggest that the cfl1l expression in the developing pouches might be dispensable for regulating actin cytoskeleton dynamics in pouch-forming cells.

• Journal of Microbiology and Biotechnology
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• 제33권3호
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• pp.339-347
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• 2023

Transforming growth factor-β is a key factor in regulating adhesion formation during tendon healing. We investigated the effectiveness of SMAD family members, SMAD7 and SMAD3, in the TGF-β/Smad signaling during flexor tendon repair. Mouse flexor toe deep tendon rupture anastomosis models were made. On days 3, 7, 14, 21, and 28, the expressions of smad7 and smad3 in flexor tendon tissues were detected by RT-qPCR and western blot. Furthermore, postoperative intraperitoneal injections of SMAD7 agonists or SMAD3 antagonists were given. The degree of tendon healing was evaluated by adhesion testing and biomechanical experiments. Hematoxylin and eosin (HE) staining was used to observe the pathological changes. Immunohistochemistry was used to evaluate the expressions of collagen III, SMAD3, and SMAD7. The mRNA levels of matrix metalloproteinases, Mmp2 and Mmp9, and scleraxis (SCX) in flexor tendon tissue were detected by RT-qPCR. Smad3 expression increased and Smad7 expression decreased in flexor tendon tissue after injury. In addition, the SMAD7 agonist blocked SMAD3 phosphorylation. SMAD7 agonist and SMAD3 antagonist both improved adhesion formation during flexor tendon healing, and decreased the expressions of collagen III, Mmp9, and SCX, while increasing Mmp2 expression. This study provides a possible theoretical basis for the SMAD7-SMAD3 signal cascade during flexor tendon adhesion healing.

• 디지털융복합연구
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• 제16권1호
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• pp.399-408
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• 2018

본 연구는 자녀가치의 매개효과를 중심으로 아버지의 원가족 경험이 양육태도와 양육참여에 미치는 영향을 알아보는데 목적이 있다. 이를 위하여 만3-5세 자녀를 둔 아버지 247명을 대상으로 원가족 경험, 자녀가치, 양육태도, 양육참여 정도를 조사하였다. 수집된 자료는 SPSS 21 program을 이용하여 기술통계, 상관분석, 회귀분석 및 Sobel Tast를 실시하였다. 연구결과, 원가족 경험과 자녀가치 중 정서적 자녀가치, 양육태도 및 양육참여는 유의한 정적 상관관계가 있었다. 매개효과를 확인하기 위하여 단계별로 회귀분석과 Sobel Test를 실시하였다. 연구 결과 아버지의 원가족 경험은 양육태도와 양육참여에 정(+)의 방향으로 영향을 미치는 것으로 확인하였고, 자녀가치 중 정서적 자녀가치가 이들 관계를 매개하는 것으로 나타났다. 이를 바탕으로 본 연구는 아버지의 긍정적 자녀가치 형성을 위한 다각적 고찰과 체계적인 지원이 필요하며 공동양육자로서의 아버지에 대한 후속연구의 방향을 제안하였다.

• 한국사회복지학
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• 제53권
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• pp.51-73
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• 2003

최근 가족해체의 급격한 증가는 한국사회에 모자가정의 빈곤화라는 심각한 사회문제를 던져주고 있다. 그러나 불행히도 한국사회에서 가족해체로 인한 모자가정의 빈곤화에 대한 사회정책의 가시적 대응은 전무한 실정이다. 이러한 현실에 근거하여 본 연구는 가족구조의 변화 즉, 이혼 및 별거 등으로 야기되는 가족해체로 인한 모자가정의 빈곤문제에 대한 정책적 대응으로서 사회안전망(social safety net)의 역할에 대해 검토하고자 하였다. 현실적으로 한국에서 종단적 자료에 대한 접근이 불가능한 상황 속에서 미국의 자료를 이용하여 미국 사회안전망의 역할을 검토하고 이에 대한 한국적 함의를 전달하고자 하였다. 미국사회에서 가족해체로 인한 모자가정의 빈곤화의 주된 원인인 가족해체로 인하여 많은 수의 모자 가정이 새로운 빈곤층으로 편입되고 있는 현실에 반해 이에 대회 사회안전망의 역할의 부재를 지적할 수 있을 것이다. 구체적으로 모자가정의 긴급한 요구에 대한 공공부조 개입의 지체, 낮은 급여수준, 불충분한 아동양육비 수준 등과 같은 부적절하고 비효율적인 사회안전망으로 인해 모자가정의 빈곤화가 가족해체이후 심화되는 것으로 나타났다. 이러한 미국의 실패가 한국에 주는 함의는 모자가정 빈곤 문제를 접근함에 있어 사회구조적 접근과 함께 가족해체로 인한 요인을 고려하여 해체가족을 위한 사회안전망의 수립이 요구된다 하겠다.

• Journal of Microbiology and Biotechnology
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• 제8권5호
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• pp.471-477
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• 1998

Taq DNA polymerase from Thermus aquaticus is very useful in the polymerase chain reaction. Taq DNA polymerase is classified in the pol I family, represented by E. coli DNA polymerase I. The three-dimensional structural alignment of 3'-5'exonuclease domains from the pol I family DNA polymerases explains why Taq DNA polymerase does not carry out proofreading in polymerase chain reactions. Three sequence motifs, Exo I, II, and III, must exist to carry out 3'-5'exonuclease activity for proof- reading by a 3'-5'exonuclease reaction, but these are abolished in Taq DNA polymerase. The key catalytic module in 3'-5'exonuclease is two metal ions chelated by four active-site carboxylic amino acids. Taq DNA polymerase was mutagenized to construct the catalytic module in the active site. The circular dichroism technique supported the formation of the catalytic module, and the radioactive assay showed that the 3'-5'exonuclease activity doubled in the mutant Taq DNA polymerase.

• Genomics & Informatics
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• 제8권4호
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• pp.201-205
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• 2010

The H1 histone family, member N, testis-specific (H1FNT) is exclusively expressed in the testis, and had its possible role for sperm chromatin formation. The purpose of this study is to investigate any genetic association of H1FNT gene with male infertility, especially at the promoter region. We examined the promoter single nucleotide polymorphisms (SNP) of H1FNT gene which is located within transcription factor binding site for its association with male infertility. The statistical analysis showed that the -1129A>T polymorphism was present at a statistically significance in male infertility (p=0.0059 and 0.0349 for hetero and risk type, respectively). The dual-luciferase promoter assay was performed to examine the polymorphic effect of this promoter SNP by the cloning of promoter region (1700bp fragment) into pGL3-basic vector. In our plasmid based reporter system, there is no big difference between wild and risk type. In conclusion, H1FNT -1129A>T promoter SNP is statistically significant with male infertility, especially with subfertile (non-azoospermia) group. Further analysis of its functional polymorphic effect in vivo may provide the biological significance of testis-specific histone with spermatogenesis.

• Molecules and Cells
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• 제37권6호
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• pp.441-448
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• 2014

Inflammasomes are specialized signaling platforms critical for the regulation of innate immune and inflammatory responses. Various NLR family members (i.e., NLRP1, NLRP3, and IPAF) as well as the PYHIN family member AIM2 can form inflammasome complexes. These multiprotein complexes activate inflammatory caspases (i.e., caspase-1) which in turn catalyze the maturation of select pro-inflammatory cytokines, including interleukin (IL)-$1{\beta}$ and IL-18. Activation of the NLRP3 inflammasome typically requires two initiating signals. Toll-like receptor (TLR) and NOD-like receptor (NLR) agonists activate the transcription of pro-inflammatory cytokine genes through an NF-${\kappa}B$-dependent priming signal. Following exposure to extracellular ATP, stimulation of the P2X purinoreceptor-7 ($P2X_7R$), which results in $K^+$ efflux, is required as a second signal for NLRP3 inflammasome formation. Alternative models for NLRP3 activation involve lysosomal destabilization and phagocytic NADPH oxidase and /or mitochondria-dependent reactive oxygen species (ROS) production. In this review we examine regulatory mechanisms that activate the NLRP3 inflammasome pathway. Furthermore, we discuss the potential roles of NLRP3 in metabolic and cognitive diseases, including obesity, type 2 diabetes mellitus, Alzheimer's disease, and major depressive disorder. Novel therapeutics involving inflammasome activation may result in possible clinical applications in the near future.

• 대한가정학회지
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• 제38권10호
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• pp.53-68
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• 2000

Children team about their roles through literature, receive the information about the roles of family members and come to accept them. Especially, father's roles in biographies are so diverse, not consistent, compared to mother's roles, that it world be judged to be difficult for the children who had read to form the concept of the father's roles. As the father's roles in the fairy tales have important effect upon the formation of the children's role concept toward their fathers, the purpose of this study is to investigate the father' role appeared in Korean biographies. The method used for this study is the content analysis of the context related with fathering in Korean biographies. It is found that the most frequent father' role appeared in Korean biographies was a friend and companion such as to talk with child about the child's future. And the second frequent father's role in Korean biographies was a family ancestor, such as, to pray for a son's birth, to name the child, to arrange the child's marriage. The third role was that of a loaming helper. These results have been discussed in the light of former studies, and the limits of study method also have been discussed.

• 한국조리학회지
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• 제4권
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• pp.147-166
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• 1998

Following the developing tendency of the food service industry, people are showing a high interest in cooking and the number of applicant trying to learn how to cook is increasing gradually. In this research, we have concluded that developing a menu, we are to standardize its cost accounting correctly so as to reduce problems which can occur during the process to commercialize food. We have to research on the quality of food changing so fast and to develop proper food to suit both the oriental and occidental taste. Since fresh ingredients such as olive oil which has much unsaturated fatty acid, tomato, and fish etc. is usually used in making Italian food, especially the moderns love it very much. The reason is that eating fatty food such as meat and fish with tomato, we can digest it well in our stomach. Tomato play an important role to neutralize the effects of acid food. It has Vitamin B1 dissolving fat, rutin strengthening capillary vessels and controlling high blood pressure, amino acid developing mental activity, iron essential to blood formation, and calcium etc. These ingredients play important roles to control geriatric diseases. The new style of the propensity to consume in the class of new generation and new family, the trend towards the nuclear family, and the rapid expansion of aging have influences on westernizing diet and on spreading new sense of values such as rapidness, convenience, cleanliness, and simplicity for diet. Following the change of the times, the food service industry will be developed more and more.

• Molecules and Cells
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• 제43권2호
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• pp.99-106
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• 2020

Cells are constantly exposed to endogenous and exogenous stresses that can result in DNA damage. In response, they have evolved complex pathways to maintain genomic integrity. RUNX family transcription factors (RUNX1, RUNX2, and RUNX3 in mammals) are master regulators of development and differentiation, and are frequently dysregulated in cancer. A growing body of research also implicates RUNX proteins as regulators of the DNA damage response, often acting in conjunction with the p53 and Fanconi anemia pathways. In this review, we discuss the functional role and mechanisms involved in RUNX factor mediated response to DNA damage and other cellular stresses. We highlight the impact of these new findings on our understanding of cancer predisposition associated with RUNX factor dysregulation and their implications for designing novel approaches to prevent cancer formation in affected individuals.