• BMB Reports
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• 제50권3호
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• pp.126-131
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• 2017

P48 Ebp1 is expressed in rapidly proliferating cells such as cancer cells and accelerates cell growth and survival. However, its expression pattern and role in central nervous system development have not been studied. Here, we demonstrated the spatiotemporal expression pattern of p48 Ebp1 during embryonic development and the postnatal period. During embryonic development, p48 Ebp1 was highly expressed in the brain. Expression gradually decreased after birth but was still more abundant than p42 expression after birth. Strikingly, we found that p48 Ebp1 was expressed in a cell type specific manner in neurons but not astrocytes. Moreover, p48 Ebp1 physically interacted with beta tubulin but not alpha tubulin. This fits with its accumulation in distal microtubule growth cone regions. Furthermore, in injured hippocampal slices, p48 Ebp1 introduction promoted axon regeneration. Thus, we speculate that p48 Ebp1 might contribute to microtubule dynamics acting as an MAP and promotes CNS axon regeneration.

• BMB Reports
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• 제41권7호
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• pp.529-536
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• 2008

Cinnamate-4-hydroxylase (C4H) is a key enzyme in the phenylpropanoid pathway, which is responsible for synthesizing a variety of secondary metabolites that participate in development and adaptation. In this study, we isolated a full-length cDNA of the C4H gene from the Korean black raspberry (Rubus sp.) and found that this gene existed as a single gene. By comparing the deduced amino acid sequence of Rubus sp. C4H with other sequences reported previously we determined that this sequence was highly conserved among widely divergent plant species. In addition, quantitative real time PCR studies indicated that the C4H gene had a differential expression pattern during fruit development, where gene expression was first detected in green fruit and was then remarkably reduced in yellow fruit, followed by an increase in red and black fruit. To investigate the two peaks in expression observed during fruit development and ripening, we measured the flavonoid content. The content of the major flavanol of Korean black raspberry fruits was determined to be highest at the beginning of fruit development, followed by a gradually decrease according to the developmental stages. In contrast, the content of anthocyanins during the progress of ripening was dramatically increased. Our results suggest that the C4H gene in Korean black raspberry plays a role during color development at the late stages of fruit ripening, whereas the expression of C4H gene during the early stages may be related to the accumulation of flavanols.

• Journal of Gastric Cancer
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• 제3권1호
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• pp.38-43
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• 2003

Purpose: Caspase 2, a member of the family of ICE-like proteases, is activated by the Fas pathway and induces apoptosis by triggering the caspase cascade. The purpose of this study was to determine whether the expression pattern of caspase 2 might be associated with gastric cancer development and if so, to determine to which pathologic parameter it is linked. Materials and Methods: For the construction of the gastric cancer tissue microarray, 78 paraffin-embedded tissues containing gastric cancer areas were cored 3 times and transferred to the recipient master block. The expression pattern of caspase 2 was examined on tissue microarray slides by using immunohistochemistry and was compared with pathologic parameters, including histologic type, depth of invasion, lymph node metastasis, and peritoneal dissemination. Results: Caspase 2 was expressed on superficial and foveolar epithelial cells and lymphocytes in the gastric mucosa, mainly in cytoplasm. We found loss of caspase 2 expression in 41 ($52.6\%$) of the 78 gastric cancer tissues. Statistically, histologic type and other pathologic parameters were not related with loss of caspase 2 expression Conclusion: Our findings provide enough evidence that loss of caspase 2 expression may contribute to the development of Korean gastric cancer and that it might be one of the possible escape mechanisms from apoptosis in gastric cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제14권8호
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• pp.4883-4889
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• 2013

Background: The most common incident cancer and cause of cancer-related deaths in women is breast cancer. The Myc gene is upregulated in many cancer types including breast cancer, and it is considered as a potential anti-cancer drug target. The present study was conducted to evaluate the Myc (gene and protein) expression pattern in an experimental mammary tumour model in rats. Materials and Methods: Thirty six Sprague Dawley rats were divided into: Experimental group (26 animals), which received the chemical carcinogen N-methyl nitrosourea (MNU) and a control group (10 animals), which received vehicle only. c-Myc oncoprotein and its mRNA expression pattern were evaluated using immunohistochemistry (IHC) and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), respectively, in normal rat mammary tissue and mammary tumours. The rat glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as internal control for semi-quantitative RT-PCR. Results: Histopathological examination of mammary tissues and tumours from MNU treated animals revealed the presence of premalignant lesions, benign tumours, in situ carcinomas and invasive carcinomas. Immunohistochemical evaluation of tumour tissues showed upregulation and heterogeneous cellular localization of c-Myc oncoprotein. The expression levels of c-Myc oncoprotein were significantly elevated (75-91%) in all the tumours. Semi-quantitative RT-PCR revealed increased expression of c-Myc mRNA in mammary tumours compared to normal mammary tissues. Conclusions: Further large-scale investigation study is needed to adopt this experimental rat mammary tumour model as an in vivo model to study anti-cancer strategies directed against Myc or its downstream partners at the transcriptional or post-transcriptional level.

• 한국멀티미디어학회논문지
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• 제16권12호
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• pp.1357-1367
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• 2013

최근 얼굴 표정 인식에 있어 영상 기반의 방법의 하나로서 ULBP 블록 히스토그램 피쳐와 SVM을 분류기로 사용한 연구가 수행되었다. Ojala 등에 의해 소개된 LBP는 높은 식별력과 조명의 변화에 대한 내구성과 간단한 연산 때문에 영상 인식 분야에 많이 사용되고 있다. 본 논문에서는 ULBP 블록 히스토그램을 계산함에 있어 분할 영역의 이동, 크기 변화에 더하여 미세한 특징 요소를 표현할 수 있도록 $LBP_{8,2}$과 $LBP_{8,1}$를 결합하였다. $LBP_{8,1}$ 660개, $LBP_{8,2}$ 550개의 분할 창으로부터 1210개의 ULBP 히스토그램 피쳐를 추출하고 이로부터 AdaBoost를 이용하여 50개의 약 분류기를 생성하였다. $LBP_{8,1}$와 $LBP_{8,2}$가 결합된 하이브리드 형태의 ULBP 블록 히스토그램 피쳐와 SVM 분류기를 이용함으로써 표정 인식률을 향상시킬 수 있었으며 다양한 실험을 통하여 이를 확인하였다. 본 논문에서 제안한 하이브리드 Boosted ULBP 히스토그램의 경우에 표정의 인식률이 96.3%로 가장 높은 결과를 보였으며 제안한 방법의 우수성을 확인하였다.

• 한국정보과학회논문지:소프트웨어및응용
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• 제32권6호
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• pp.570-579
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• 2005

표정인식 연구는 맨$\cdot$머신 인터페이스 개발, 개인 식별, 가상모델에 의한 표정복원 등 응용가치의 무한한 가능성과 함께 다양한 분야에서 연구되고 있다 본 논문에서는 인간의 기본정서 중 행복, 분노, 놀람, 슬픔에 대한 4가지 표정을 얼굴의 강체 움직임이 없는 얼굴동영상으로부터 간단히 표정인식 할 수 있는 방법을 제안한다. 먼저, 얼굴 및 표정을 결정하는 요소들과 각 요소의 특징영역들을 색상, 크기 그리고 위치정보를 이용하여 자동으로 검출한다. 다음으로 Gradient Method를 이용하여 추정한 광류 값으로 특징영역들에 대한 방향패턴을 결정한 후, 본 연구가 제안한 방향모델을 이용하여 방향패턴에 대한 매칭을 행한다. 각 정서를 대표하는 방향모델과의 패턴 매칭에서 그 조합 값이 최소를 나타내는 부분이 가장 유사한 정서임을 판단하고 표정인식을 행한다. 마지막으로 실험을 통하여 본 논문의 유효성을 확인한다.

• Fisheries and Aquatic Sciences
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• 제15권3호
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• pp.221-231
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• 2012

The marine medaka Oryzias dancena choriogenin H gene (odChgH) and its mRNA expression during estradiol-$17{\beta}$ (E2) exposure were characterized. At the amino acid level, the choriogenin H protein is predicted to possess the conserved repetitive N-terminal region, as well as zona pellucida (ZP) and Trefoil factor family (TFF) domains. At the genomic level, odChgH has an eight-exon organization with a distribution pattern of transcription factor binding sites in the 5'-upstream region, which is commonly found in other estrogen-responsive genes. The tissue distribution pattern of odChgH mRNA was found to be gender-specific, whereby females showed a higher expression level and wider tissue distribution than did males. During embryonic development, odChgH mRNA was robustly detected from the stage of visceral blood vessel formation. Experimental E2 exposure of males resulted in odChgH mRNA being induced not only in the liver, but also in other several tissues. The E2-mediated induction was fairly dose-dependent. The basal expression levels of hepatic odChgH mRNA were lower in males that were acclimated to 30 ppt salinity than in those acclimated to 0 or 15 ppt salinity. In contrast, the inducibility of odChgH mRNA during E2 exposure was greater in seawater-acclimated fish than in brackish water- or freshwater-acclimated fish.

• 전자공학회논문지CI
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• 제46권6호
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• pp.44-55
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• 2009

최근 활발히 연구가 진행 중인 마이크로어레이로부터 얻어지는 유전자 발현 데이터를 이용한 질병 진단은, 데이터를 직접적으로 분석하기 힘들기 때문에 일반적으로 기계 학습 알고리즘을 사용하여 이루어져왔다. 그러나 유전자 발현 데이터를 분석함에 있어서 유전자들 간의 상호작용을 고려하는 분석이 필요하다는 최근의 연구 결과들은 기존 기계 학습 알고리즘들을 이용한 분석에 한계가 있음을 의미한다고 볼 수 있다. 본 논문에서는 특징들 사이의 고차원 상관관계를 고려 가능한 하이퍼네트워크 모델을 이용하여 유전자 발현 데이터의 분류를 수행하고 기존의 기계 학습 알고리즘들과 분류 성능을 비교한다. 또한 기존 하이퍼네트워크 모델의 단점을 개선 한 모델을 제안하고, 이를 병렬 프로세서 상에서 구현하여 처리 성능을 비교한다. 실험 결과 제안 된 모델은 기존의 기계 학습 방법들과의 비교에서도 경쟁력 있는 분류 성능을 보여주었고, 기존 하이퍼네트워크 모델 보다 안정적이고 향상된 분류 성능을 보여주었다. 또한 이를 병렬 프로세서 상에서 구현 할 경우 처리 성능을 극대화 할 수 있음을 보였다.

• 한국콘텐츠학회논문지
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• 제13권8호
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• pp.466-473
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• 2013

유전자 염기서열의 직접적인 변화 대신 염기의 수정 또는 변형을 통해 유전자 발현이 조절되는 후성유전은 크게 DNA 메틸화(methylation), 히스톤 변형(modification), ncRNA(non-coding RNA)에 의해 제어가 가능하다. 본 연구에서는 후성유전을 이해하기 위해 노화 관련 유전자를 대상으로 데이터베이스를 구축하고, DNA 메틸화를 중심으로 후성 유전학적 특성을 분석하였다. 유전자의 upstream 부위와 프로모터(promoter) 부위에 있는 CpG island(CGI)에 메틸화가 될 경우 유전자 발현을 억제하기 때문에 CGI를 중심으로 전체 유전자 그룹과 노화 관련 유전자 그룹간의 분포도를 비교 분석하였다. 또한 메틸화와 관련된 CGI로부터 얻은 메틸화 관련 motif 패턴을 이용하여 노화 유전자와의 관계를 분석하였다. 노화 관련 유전자의 CGI 분포는 전사인자 결합자리의 분포와 일치하였다. 본 연구에서 제공하는 DNA 메틸화 중심의 후성유전학적 정보는 노화 관련 유전자의 조절과 노화를 이해하는데 도움이 될 것으로 사료된다.

• 한국패류학회지
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• 제31권3호
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• pp.233-241
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• 2015

Toll-like receptors (TLRs) are a major pattern recognition receptor that recognize the structure of invading pathogen and play key roles by triggering immune response. In this study, we identified a sequence of TLR homolog and characterized at molecular level from the abalone (Haliotis discus hannai). Multiple alignments and phylogenetic analysis of abalone TLR protein belongs to the TLR 2/6. Expression level of abalone TLR 2/6 in the tissue was comparatively high in the mantle, gill, digestive duct, and hemocytes, but lowest in the muscle. Expression level of abalone TLR 2/6 mRNA in the mantle, gill, digestive duct, and hemocytes was 20-fold, 60-fold, 115-fold, 112-fold higher than in the muscle, respectively. Expression level of abalone TLR 2/6 mRNA in the mantle was steadily increased until 12 h and decreased post-infection with Vibrio parahemolyticus. While the expression level of abalone TLR 2/6 mRNA in the gill and hemocytes was drastically increased at 6 and 9 h post-infection with Vibrio parahemolyticus, respectively. These results suggest that abalone TLR 2/6 is conserved through evolution and may play roles similar to its mammalian counterparts.