The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Molecular Characterization and Expression Analysis of a Toll-like receptor 2/6 gene from Abalone (Haliotis discus hannai)

북방전복 (Haliotis discus hannai) 에서 분리한 Toll-like receptor 2/6 유전자의 분자생물학적 특성 및 발현분석

  • 문지영 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 박은희 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 공희정 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 김영옥 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 김동균 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 안철민 (국립수산과학원 전략양식연구소 생명공학과) ;
  • 남보혜 (국립수산과학원 전략양식연구소 생명공학과)
  • Received : 2015.09.11
  • Accepted : 2015.09.30
  • Published : 2015.09.30
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Abstract

Toll-like receptors (TLRs) are a major pattern recognition receptor that recognize the structure of invading pathogen and play key roles by triggering immune response. In this study, we identified a sequence of TLR homolog and characterized at molecular level from the abalone (Haliotis discus hannai). Multiple alignments and phylogenetic analysis of abalone TLR protein belongs to the TLR 2/6. Expression level of abalone TLR 2/6 in the tissue was comparatively high in the mantle, gill, digestive duct, and hemocytes, but lowest in the muscle. Expression level of abalone TLR 2/6 mRNA in the mantle, gill, digestive duct, and hemocytes was 20-fold, 60-fold, 115-fold, 112-fold higher than in the muscle, respectively. Expression level of abalone TLR 2/6 mRNA in the mantle was steadily increased until 12 h and decreased post-infection with Vibrio parahemolyticus. While the expression level of abalone TLR 2/6 mRNA in the gill and hemocytes was drastically increased at 6 and 9 h post-infection with Vibrio parahemolyticus, respectively. These results suggest that abalone TLR 2/6 is conserved through evolution and may play roles similar to its mammalian counterparts.

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References

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